Published in J Mol Biol on June 13, 2003
Thermodynamic system drift in protein evolution. PLoS Biol (2014) 2.10
How fast is protein hydrophobic collapse? Proc Natl Acad Sci U S A (2003) 2.01
Lessons in stability from thermophilic proteins. Protein Sci (2006) 1.88
Role of residual structure in the unfolded state of a thermophilic protein. Proc Natl Acad Sci U S A (2003) 1.27
Exploring protein-folding ensembles: a variable-barrier model for the analysis of equilibrium unfolding experiments. Proc Natl Acad Sci U S A (2004) 1.21
Conserved quantitative stability/flexibility relationships (QSFR) in an orthologous RNase H pair. Proteins (2006) 1.16
The efficiency of different salts to screen charge interactions in proteins: a Hofmeister effect? Biophys J (2004) 1.13
Entropic stabilization of proteins and its proteomic consequences. PLoS Comput Biol (2005) 1.11
Electrostatic interactions in the denatured state ensemble: their effect upon protein folding and protein stability. Arch Biochem Biophys (2007) 1.01
Changes in Lysozyme Flexibility upon Mutation Are Frequent, Large and Long-Ranged. PLoS Comput Biol (2012) 0.96
Structure, stability, and folding of ribonuclease H1 from the moderately thermophilic Chlorobium tepidum: comparison with thermophilic and mesophilic homologues. Biochemistry (2009) 0.94
The unfolded state of the C-terminal domain of the ribosomal protein L9 contains both native and non-native structure. Biochemistry (2009) 0.93
Native state energetics of the Src SH2 domain: evidence for a partially structured state in the denatured ensemble. Protein Sci (2006) 0.92
A designed protein as experimental model of primordial folding. Proc Natl Acad Sci U S A (2009) 0.87
Large-scale modulation of thermodynamic protein folding barriers linked to electrostatics. Proc Natl Acad Sci U S A (2008) 0.86
Increasing protein stability: importance of DeltaC(p) and the denatured state. Protein Sci (2010) 0.85
Rational modification of protein stability by targeting surface sites leads to complicated results. Proc Natl Acad Sci U S A (2013) 0.84
Explanation of the stability of thermophilic proteins based on unique micromorphology. Biophys J (2006) 0.84
Energetically significant networks of coupled interactions within an unfolded protein. Proc Natl Acad Sci U S A (2014) 0.83
Direct observation of the three-state folding of a single protein molecule. Science (2005) 4.48
Experimental identification of downhill protein folding. Science (2002) 2.43
Pulse proteolysis: a simple method for quantitative determination of protein stability and ligand binding. Nat Methods (2005) 2.18
Probing the chemistry of thioredoxin catalysis with force. Nature (2007) 2.17
Protein folding: defining a "standard" set of experimental conditions and a preliminary kinetic data set of two-state proteins. Protein Sci (2005) 1.91
The folding cooperativity of a protein is controlled by its chain topology. Nature (2010) 1.76
A Ras-induced conformational switch in the Ras activator Son of sevenless. Proc Natl Acad Sci U S A (2006) 1.57
Probing the high energy states in proteins by proteolysis. J Mol Biol (2004) 1.51
A course-based research experience: how benefits change with increased investment in instructional time. CBE Life Sci Educ (2014) 1.37
Estimating free-energy barrier heights for an ultrafast folding protein from calorimetric and kinetic data. J Phys Chem B (2008) 1.34
Stepwise protein folding at near amino acid resolution by hydrogen exchange and mass spectrometry. Proc Natl Acad Sci U S A (2013) 1.34
Single-molecule paleoenzymology probes the chemistry of resurrected enzymes. Nat Struct Mol Biol (2011) 1.32
Hyperstability and substrate promiscuity in laboratory resurrections of Precambrian β-lactamases. J Am Chem Soc (2013) 1.32
Protein-DNA chimeras for single molecule mechanical folding studies with the optical tweezers. Eur Biophys J (2008) 1.29
Role of residual structure in the unfolded state of a thermophilic protein. Proc Natl Acad Sci U S A (2003) 1.27
Exploring protein-folding ensembles: a variable-barrier model for the analysis of equilibrium unfolding experiments. Proc Natl Acad Sci U S A (2004) 1.21
Exploring subdomain cooperativity in T4 lysozyme II: uncovering the C-terminal subdomain as a hidden intermediate in the kinetic folding pathway. Protein Sci (2007) 1.21
L-phenylalanine binding and domain organization in human phenylalanine hydroxylase: a differential scanning calorimetry study. Biochemistry (2002) 1.20
Destabilization of the Escherichia coli RNase H kinetic intermediate: switching between a two-state and three-state folding mechanism. J Mol Biol (2004) 1.19
Robustness of downhill folding: guidelines for the analysis of equilibrium folding experiments on small proteins. Biochemistry (2005) 1.18
Protein folding drives disulfide formation. Cell (2012) 1.18
Exploring subdomain cooperativity in T4 lysozyme I: structural and energetic studies of a circular permutant and protein fragment. Protein Sci (2007) 1.17
Energetics-based protein profiling on a proteomic scale: identification of proteins resistant to proteolysis. J Mol Biol (2007) 1.15
The efficiency of different salts to screen charge interactions in proteins: a Hofmeister effect? Biophys J (2004) 1.13
Natural selection for kinetic stability is a likely origin of correlations between mutational effects on protein energetics and frequencies of amino acid occurrences in sequence alignments. J Mol Biol (2006) 1.12
Electrostatic interactions in ubiquitin: stabilization of carboxylates by lysine amino groups. Biochemistry (2002) 1.11
Diversity of chemical mechanisms in thioredoxin catalysis revealed by single-molecule force spectroscopy. Nat Struct Mol Biol (2009) 1.11
Relation between protein stability, evolution and structure, as probed by carboxylic acid mutations. J Mol Biol (2004) 1.10
The molten globule state is unusually deformable under mechanical force. Proc Natl Acad Sci U S A (2012) 1.07
Quantitative determination of protein stability and ligand binding by pulse proteolysis. Curr Protoc Protein Sci (2006) 1.07
On the precision of experimentally determined protein folding rates and phi-values. Protein Sci (2006) 1.06
Direct observation of a force-induced switch in the anisotropic mechanical unfolding pathway of a protein. Proc Natl Acad Sci U S A (2012) 1.05
Experimental evaluation of topological parameters determining protein-folding rates. Proc Natl Acad Sci U S A (2002) 1.05
Differences between the prion protein and its homolog Doppel: a partially structured state with implications for scrapie formation. J Mol Biol (2002) 1.04
Role of solvation barriers in protein kinetic stability. J Mol Biol (2006) 1.04
Expanding the realm of ultrafast protein folding: gpW, a midsize natural single-domain with alpha+beta topology that folds downhill. J Am Chem Soc (2008) 1.03
Comparison of the folding processes of T. thermophilus and E. coli ribonucleases H. J Mol Biol (2002) 1.01
Limitations of constant-force-feedback experiments. Biophys J (2012) 1.01
Contributions of folding cores to the thermostabilities of two ribonucleases H. Protein Sci (2002) 0.99
Thermodynamic and kinetic characterization of a germ line human lambda6 light-chain protein: the relation between unfolding and fibrillogenesis. J Mol Biol (2009) 0.98
Comparison of proteolytic susceptibility in phosphoglycerate kinases from yeast and E. coli: modulation of conformational ensembles without altering structure or stability. J Mol Biol (2007) 0.97
Direct measurement of barrier heights in protein folding. J Am Chem Soc (2005) 0.97
Human cystathionine β-synthase (CBS) contains two classes of binding sites for S-adenosylmethionine (SAM): complex regulation of CBS activity and stability by SAM. Biochem J (2013) 0.96
Modulation of buried ionizable groups in proteins with engineered surface charge. J Am Chem Soc (2010) 0.95
Hydrogen exchange and ligand binding: ligand-dependent and ligand-independent protection in the Src SH3 domain. Protein Sci (2004) 0.94
Structure, stability, and folding of ribonuclease H1 from the moderately thermophilic Chlorobium tepidum: comparison with thermophilic and mesophilic homologues. Biochemistry (2009) 0.94
Between-species variation in the kinetic stability of TIM proteins linked to solvation-barrier free energies. J Mol Biol (2008) 0.93
Role of low native state kinetic stability and interaction of partially unfolded states with molecular chaperones in the mitochondrial protein mistargeting associated with primary hyperoxaluria. Amino Acids (2010) 0.92
Native state energetics of the Src SH2 domain: evidence for a partially structured state in the denatured ensemble. Protein Sci (2006) 0.92
Dwell time analysis of a single-molecule mechanochemical reaction. Langmuir (2007) 0.91
Engineering proteins with tunable thermodynamic and kinetic stabilities. Proteins (2008) 0.91
On the mechanism of chaperone activity of the small heat-shock protein of Methanococcus jannaschii. Proc Natl Acad Sci U S A (2003) 0.90
Phenotypic comparisons of consensus variants versus laboratory resurrections of Precambrian proteins. Proteins (2014) 0.89
A hinge region cis-proline in ribonuclease A acts as a conformational gatekeeper for C-terminal domain swapping. J Mol Biol (2010) 0.89
Analysis of the stability of multimeric proteins by effective DeltaG and effective m-values. Protein Sci (2004) 0.89
Propagation of a single destabilizing mutation throughout the Escherichia coli ribonuclease HI native state. Protein Sci (2002) 0.87
Conservation of protein structure over four billion years. Structure (2013) 0.87
Structural and kinetic mapping of side-chain exposure onto the protein energy landscape. Proc Natl Acad Sci U S A (2011) 0.87
pH corrections in chemical denaturant solutions. Anal Biochem (2002) 0.87
Force-clamp spectroscopy detects residue co-evolution in enzyme catalysis. J Biol Chem (2008) 0.87
A designed protein as experimental model of primordial folding. Proc Natl Acad Sci U S A (2009) 0.87
The folding trajectory of RNase H is dominated by its topology and not local stability: a protein engineering study of variants that fold via two-state and three-state mechanisms. J Mol Biol (2009) 0.87
DNA molecular handles for single-molecule protein-folding studies by optical tweezers. Methods Mol Biol (2011) 0.86
Large-scale modulation of thermodynamic protein folding barriers linked to electrostatics. Proc Natl Acad Sci U S A (2008) 0.86
A single mutation at residue 25 populates the folding intermediate of E. coli RNase H and reveals a highly dynamic partially folded ensemble. J Mol Biol (2009) 0.86
Kinetic evidence for a two-stage mechanism of protein denaturation by guanidinium chloride. Proc Natl Acad Sci U S A (2014) 0.85
The effect of charge-introduction mutations on E. coli thioredoxin stability. Biophys Chem (2004) 0.85
A stability pattern of protein hydrophobic mutations that reflects evolutionary structural optimization. Biophys J (2005) 0.84
Protein folding and unfolding under force. Biopolymers (2013) 0.84
Energetic and structural consequences of desolvation/solvation barriers to protein folding/unfolding assessed from experimental unfolding rates. Biophys J (2006) 0.84
Hydrogen-exchange strategies applied to energetics of intermediate processes in protein folding. Methods Enzymol (2004) 0.84
Using multi-objective computational design to extend protein promiscuity. Biophys Chem (2010) 0.83
A simple tool to explore the distance distribution of correlated mutations in proteins. Biophys Chem (2005) 0.82
Identification of residual structure in the unfolded state of ribonuclease H1 from the moderately thermophilic Chlorobium tepidum: comparison with thermophilic and mesophilic homologues. Biochemistry (2010) 0.82
Navigating the downhill protein folding regime via structural homologues. J Am Chem Soc (2010) 0.82
Structural and stability effects of phosphorylation: Localized structural changes in phenylalanine hydroxylase. Protein Sci (2004) 0.82
Propensity for C-terminal domain swapping correlates with increased regional flexibility in the C-terminus of RNase A. Protein Sci (2011) 0.82
Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering. Appl Microbiol Biotechnol (2014) 0.82
Empirical parametrization of pK values for carboxylic acids in proteins using a genetic algorithm. Biophys Chem (2004) 0.81
Do proteins always benefit from a stability increase? Relevant and residual stabilisation in a three-state protein by charge optimisation. J Mol Biol (2004) 0.81
Mechanism of protein kinetic stabilization by engineered disulfide crosslinks. PLoS One (2013) 0.80
Highly anomalous energetics of protein cold denaturation linked to folding-unfolding kinetics. PLoS One (2011) 0.80
The peripheral binding of 14-3-3γ to membranes involves isoform-specific histidine residues. PLoS One (2012) 0.80
Role of conservative mutations in protein multi-property adaptation. Biochem J (2010) 0.80
Proteolytic scanning calorimetry: a novel methodology that probes the fundamental features of protein kinetic stability. Biophys J (2010) 0.80
Probing the mutational interplay between primary and promiscuous protein functions: a computational-experimental approach. PLoS Comput Biol (2012) 0.79
Thermostable and promiscuous Precambrian proteins. Environ Microbiol (2014) 0.79
Different contribution of conserved amino acids to the global properties of triosephosphate isomerases. Proteins (2013) 0.78
Estimation of protein folding free energy barriers from calorimetric data by multi-model Bayesian analysis. Phys Chem Chem Phys (2011) 0.78
Protein-protein binding affinities by pulse proteolysis: application to TEM-1/BLIP protein complexes. Protein Sci (2010) 0.78
Mono and dual cofactor dependence of human cystathionine β-synthase enzyme variants in vivo and in vitro. G3 (Bethesda) (2013) 0.77
How many ionizable groups can sit on a protein hydrophobic core? Proteins (2011) 0.77
Molecular determinants of expansivity of native globular proteins: a pressure perturbation calorimetry study. J Phys Chem B (2014) 0.77
Kinetic analysis and modelling of the allosteric behaviour of liver and muscle glycogen phosphorylases. J Mol Recognit (2006) 0.77
Beyond Lumry-Eyring: an unexpected pattern of operational reversibility/irreversibility in protein denaturation. Proteins (2008) 0.76
Are protein folding intermediates the evolutionary consequence of functional constraints? J Phys Chem B (2015) 0.76
Energetics of heparin binding to human acidic fibroblast growth factor. Int J Biol Macromol (2002) 0.76
Single molecule conformational memory extraction: p5ab RNA hairpin. J Phys Chem B (2014) 0.75