Published in Proc Natl Acad Sci U S A on June 01, 1992
Characteristics of nearly dry enzymes in organic solvents: implications for biocatalysis in the absence of water. Philos Trans R Soc Lond B Biol Sci (2004) 1.06
Conformational sampling of peptides in cellular environments. Biophys J (2007) 1.04
Modeling structure and flexibility of Candida antarctica lipase B in organic solvents. BMC Struct Biol (2008) 1.00
What can we learn by studying enzymes in non-aqueous media? Philos Trans R Soc Lond B Biol Sci (2004) 0.88
Water dynamics and salt-activation of enzymes in organic media: mechanistic implications revealed by NMR spectroscopy. Proc Natl Acad Sci U S A (2006) 0.86
Biocatalyst activity in nonaqueous environments correlates with centisecond-range protein motions. Proc Natl Acad Sci U S A (2008) 0.85
Conformational changes of alpha-chymotrypsin in a fibrillation-promoting condition: a molecular dynamics study. Biophys J (2008) 0.82
Role of α-helical structure in organic solvent-activated homodimer of elastase strain K. Int J Mol Sci (2011) 0.81
Hydrogen/deuterium exchange study of subtilisin Carlsberg during prolonged exposure to organic solvents. Biotechnol Bioeng (2009) 0.81
Eliminating hydrolytic activity without affecting the transglycosylation of a GH1 β-glucosidase. Appl Microbiol Biotechnol (2016) 0.80
Predicting enzyme behavior in nonconventional media: correlating nitrilase function with solvent properties. J Ind Microbiol Biotechnol (2008) 0.78
Synthesis of fatty acid esters and diacylglycerols at elevated temperatures by alkalithermophilic lipases from Thermosyntropha lipolytica. J Ind Microbiol Biotechnol (2009) 0.76
Lipase in aqueous-polar organic solvents: activity, structure, and stability. Protein Sci (2013) 0.75
To Keep or Not to Keep? The Question of Crystallographic Waters for Enzyme Simulations in Organic Solvent. Mol Simul (2016) 0.75
Biocatalytic synthesis of acrylates in supercritical fluids: tuning enzyme activity by changing pressure. Proc Natl Acad Sci U S A (1993) 0.75
Examination of the quality of various force fields and solvation models for the equilibrium simulations of GA88 and GB88. J Mol Model (2016) 0.75
Calculations of electrostatic interactions in biological systems and in solutions. Q Rev Biophys (1984) 5.18
Electrostatic effects in proteins. Science (1978) 5.02
Molecular dynamics simulations in biology. Nature (1990) 2.95
Enzymatic catalysis in nonaqueous solvents. J Biol Chem (1988) 2.23
Structure of alpha-chymotrypsin refined at 1.68 A resolution. J Mol Biol (1985) 2.08
Enzymatic catalysis in organic media at 100 degrees C. Science (1984) 2.00
Enzymatic catalysis and dynamics in low-water environments. Proc Natl Acad Sci U S A (1992) 1.66
Mapping protein dynamics by X-ray diffraction. Prog Biophys Mol Biol (1985) 1.49
Inelastic neutron scattering analysis of picosecond internal protein dynamics. Comparison of harmonic theory with experiment. J Mol Biol (1988) 1.46
Protein structure determination in solution by nuclear magnetic resonance spectroscopy. Science (1989) 1.22
Enzymatic oxidation of cholesterol aggregates in supercritical carbon dioxide. Science (1988) 1.09
The modelling of electrostatic interactions in the function of globular proteins. Prog Biophys Mol Biol (1986) 1.05
Time-domain reflectrometry studies of water binding and structural flexibility in chymotrypsin. Biochim Biophys Acta (1987) 0.97
Spin-labeled sulfonyl fluorides as active site probes of protease structure. I. Comparison of the active site environments in alpha-chymotrypsin and trypsin. J Biol Chem (1974) 0.86
Spin-labeled sulfonyl fluorides as active site probes of protease structure. II. Spin label syntheses and enzyme inhibition. J Biol Chem (1974) 0.82
Electron paramagnetic resonance studies of immobilized chymotrypsin. Methods Enzymol (1987) 0.81
ESR probing of macromolecules: spin label study of the conformational integrity and transitions in modified alpha-chymotrypsin. Arch Biochem Biophys (1972) 0.81
Comparison of pour and surface plate methods for determination of bacterial counts. Can J Microbiol (1967) 4.48
Linkage studies in familial Alzheimer disease: evidence for chromosome 19 linkage. Am J Hum Genet (1991) 4.19
Factors affecting rate of methane formation from acetic acid by enriched methanogenic cultures. Can J Microbiol (1976) 2.84
Crystal structure of enteropathogenic Escherichia coli intimin-receptor complex. Nature (2000) 2.21
Characterization of a strain of Methanospirillum hungatti. Can J Microbiol (1976) 2.16
Enzymatic catalysis and dynamics in low-water environments. Proc Natl Acad Sci U S A (1992) 1.66
Torsades de pointes associated with high dose levomethadyl acetate (ORLAAM). J Addict Dis (2001) 1.65
Binding of the cellulose-binding domain of exoglucanase Cex from Cellulomonas fimi to insoluble microcrystalline cellulose is entropically driven. Proc Natl Acad Sci U S A (1996) 1.51
Pressure and Temperature Effects on Growth and Methane Production of the Extreme Thermophile Methanococcus jannaschii. Appl Environ Microbiol (1988) 1.51
Surface diffusion of cellulases and their isolated binding domains on cellulose. J Biol Chem (1997) 1.50
Improvement of spray gun method of estimating bacterial populations on surfaces. Can J Microbiol (1965) 1.44
Binding specificity and thermodynamics of a family 9 carbohydrate-binding module from Thermotoga maritima xylanase 10A. Biochemistry (2001) 1.42
Analysis of binding of the family 2a carbohydrate-binding module from Cellulomonas fimi xylanase 10A to cellulose: specificity and identification of functionally important amino acid residues. Protein Eng (2000) 1.39
Examination of primary metabolic pathways in a murine hybridoma with carbon-13 nuclear magnetic resonance spectroscopy. Biotechnol Bioeng (1994) 1.37
A nuclear magnetic resonance technique for determining hybridoma cell concentration in hollow fiber bioreactors. Biotechnology (N Y) (1990) 1.30
Conformational properties of the complexes formed by proteins and sodium dodecyl sulfate. Biochemistry (1976) 1.29
Interaction of polysaccharides with the N-terminal cellulose-binding domain of Cellulomonas fimi CenC. 1. Binding specificity and calorimetric analysis. Biochemistry (1996) 1.29
Effect of cobra venom-induced inhibition of complement activity on allograft and xenograft rejection reactions. Transplantation (1967) 1.25
Quantitation of bleeding symptoms in children with von Willebrand disease: use of a standardized pediatric bleeding questionnaire. J Thromb Haemost (2010) 1.21
Characterization and affinity applications of cellulose-binding domains. J Chromatogr B Biomed Sci Appl (1998) 1.16
Effects of hyperbaric pressure on a deep-sea archaebacterium in stainless steel and glass-lined vessels. Appl Environ Microbiol (1991) 1.11
Cadmium removal by a new strain of Pseudomonas aeruginosa in aerobic culture. Appl Environ Microbiol (1997) 1.09
Enzymatic oxidation of cholesterol aggregates in supercritical carbon dioxide. Science (1988) 1.09
Long-range acoustic detection and localization of blue whale calls in the northeast Pacific Ocean. J Acoust Soc Am (1998) 1.03
Pressure stabilization of proteins from extreme thermophiles. Appl Environ Microbiol (1994) 1.02
High-pressure-temperature bioreactor for studying pressure-temperature relationships in bacterial growth and productivity. Biotechnol Bioeng (1988) 1.01
Inhibitory effect of zinc ions on platelet aggregation and serotonin release reaction. Life Sci (1975) 1.00
Humoral factors in canine renal allograft rejection. Lancet (1968) 0.99
Optimizing the salt-induced activation of enzymes in organic solvents: effects of lyophilization time and water content. Biotechnol Bioeng (1999) 0.99
Evidence for two platelet populations in xenograft rejection. Surg Forum (1966) 0.97
Normal range of bleeding scores for the ISTH-BAT: adult and pediatric data from the merging project. Haemophilia (2014) 0.97
Studies on the bacterial flora of vacuum-packaged fresh beef. Can J Microbiol (1972) 0.96
Structure and binding specificity of the second N-terminal cellulose-binding domain from Cellulomonas fimi endoglucanase C. Biochemistry (2000) 0.96
Testing for diffusion limitations in salt-activated enzyme catalysts operating in organic solvents. Biotechnol Bioeng (1998) 0.95
Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows. J Dairy Sci (2007) 0.94
Four-directional-development thin-layer chromatography of lipids using trimethyl borate. J Lipid Res (1971) 0.92
Continuous culture of Methanococcus jannaschii, an extremely thermophilic methanogen. Biotechnol Bioeng (1994) 0.92
Multinuclear NMR study of enzyme hydration in an organic solvent. Biotechnol Bioeng (1998) 0.92
Effect of lactobacilli and carbon dioxide on the growth of Microbacterium thermosphactum on fresh beef. Can J Microbiol (1975) 0.92
Aerobic sulfide production and cadmium precipitation by Escherichia coli expressing the Treponema denticola cysteine desulfhydrase gene. Appl Microbiol Biotechnol (2001) 0.91
Cholesterol aggregation and interaction with cholesterol oxidase in supercritical carbon dioxide. Proc Natl Acad Sci U S A (1988) 0.91
Pressure effects on the composition and thermal behavior of lipids from the deep-sea thermophile Methanococcus jannaschii. J Bacteriol (1995) 0.91
Driving forces for phase separation and partitioning in aqueous two-phase systems. J Chromatogr B Biomed Sci Appl (1998) 0.91
Effect of ferrocyanide on growth and citric acid production by Aspergillus niger. Can J Microbiol (1966) 0.90
Molecular cloning of extremely thermostable esterase gene from hyperthermophilic archaeon Pyrococcus furiosus in Escherichia coli. Biotechnol Bioeng (1998) 0.89
Combinatorial biocatalysis: a natural approach to drug discovery. Trends Biotechnol (1998) 0.89
Engineering considerations for the application of extremophiles in biotechnology. Crit Rev Biotechnol (1991) 0.89
High-pressure, high-temperature bioreactor for comparing effects of hyperbaric and hydrostatic pressure on bacterial growth. Appl Environ Microbiol (1992) 0.89
Quantitative in vivo nuclear magnetic resonance studies of hybridoma metabolism. Biotechnol Bioeng (1994) 0.89
Cell retention-chemostat studies of hybridoma cells-analysis of hybridoma growth and metabolism in continuous suspension culture in serum-free medium. Biotechnol Bioeng (1993) 0.88
Intrinsic effects of solvent polarity on enzymic activation energies. Biotechnol Bioeng (2000) 0.88
Towards more active biocatalysts in organic media: increasing the activity of salt-activated enzymes. Biotechnol Bioeng (2001) 0.88
Metabolic engineering of an aerobic sulfate reduction pathway and its application to precipitation of cadmium on the cell surface. Appl Environ Microbiol (2000) 0.87
Effector mechanisms in renal graft rejection. Surgery (1967) 0.87
Studies on the mechanism of canine renal allograft rejection. Transplant Proc (1969) 0.87
Binding site analysis of cellulose binding domain CBD(N1) from endoglucanse C of Cellulomonas fimi by site-directed mutagenesis. Biochemistry (2000) 0.86
Effect of azone and propylene glycol on penetration of trifluorothymidine through skin and efficacy of different topical formulations against cutaneous herpes simplex virus infections in guinea pigs. Antimicrob Agents Chemother (1984) 0.86
Performance of succinylacetone assays and their associated proficiency testing outcomes. Clin Biochem (2012) 0.86
Early dialysis in the treatment of acute renal failure. Surg Gynecol Obstet (1966) 0.86
Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli. Appl Environ Microbiol (2000) 0.85
In vitro and in vivo stability of diluted recombinant factor VIII for continuous infusion use in haemophilia A. Haemophilia (2009) 0.84
Pressure-enhanced activity and stability of a hyperthermophilic protease from a deep-sea methanogen. Appl Environ Microbiol (1997) 0.84
Calcium binding by the N-terminal cellulose-binding domain from Cellulomonas fimi beta-1,4-glucanase CenC. Biochemistry (1998) 0.84
Active-site titration of serine proteases in organic solvents. Biotechnol Bioeng (1996) 0.84
Pressure effects on enzyme activity and stability at high temperatures. Adv Protein Chem (1996) 0.83
Using isotopomer path tracing to quantify metabolic fluxes in pathway models containing reversible reactions. Biotechnol Bioeng (2001) 0.83
Transient responses of hybridoma cells in continuous culture to step changes in amino acid and vitamin concentrations. Biotechnol Bioeng (1994) 0.83
Uranyl precipitation by biomass from an enhanced biological phosphorus removal reactor. Biodegradation (2001) 0.82
Pressure stabilization is not a general property of thermophilic enzymes: the adenylate kinases of Methanococcus voltae, Methanococcus maripaludis, Methanococcus thermolithotrophicus, and Methanococcus jannaschii. Appl Environ Microbiol (1995) 0.82
Combinatorial biocatalysis: taking the lead from nature. Curr Opin Biotechnol (1999) 0.82
Mechanisms of protein solubilization in reverse micelles. Biotechnol Bioeng (1992) 0.82
A kinetic analysis of hybridoma growth and metabolism in continuous suspension culture on serum-free medium. Biotechnol Bioeng (1991) 0.82
Very high-level production and export in Escherichia coli of a cellulose binding domain for use in a generic secretion-affinity fusion system. Biotechnol Bioeng (1997) 0.82
Effect of extracellular glutamine concentration on primary and secondary metabolism of a murine hybridoma: an in vivo 13C nuclear magnetic resonance study. Biotechnol Bioeng (1998) 0.82
Analysis of an engineered sulfate reduction pathway and cadmium precipitation on the cell surface. Biotechnol Bioeng (2001) 0.82
Non-penetrating trauma to the carotid artery with secondary thrombosis and embolism: treatment by thrombolysin. Acta Neurochir (Wien) (1977) 0.81
Effect of collagen crosslinking on the rate of resorption of implanted collagen tubing in rabbits. J Biomed Mater Res (1977) 0.81
Cellulose-binding domains. Versatile affinity tags for inexpensive large-scale purification, concentration, and immobilization of fusion proteins. Ann N Y Acad Sci (1996) 0.81
The role of a conserved tyrosine residue in high-potential iron sulfur proteins. Protein Sci (1995) 0.81
Surface-density and orientation effects on immobilized antibodies and antibody fragments. Biotechnology (N Y) (1993) 0.81
Chemical denaturation and elevated folding temperatures are required for wild-type activity and stability of recombinant Methanococcus jannaschii 20S proteasome. Protein Sci (2001) 0.81
Stability and oligosaccharide binding of the N1 cellulose-binding domain of Cellulomonas fimi endoglucanase CenC. Biochemistry (1998) 0.81
Partial Purification and Characterization of Two Hydrogenases from the Extreme Thermophile Methanococcus jannaschii. Appl Environ Microbiol (1990) 0.81
Electron paramagnetic resonance studies of immobilized chymotrypsin. Methods Enzymol (1987) 0.81
Postoperative renal failure. J Lancet (1968) 0.81
Growth of psychrotolerant psudomonads and achromobacter on chicken skin. Poult Sci (1968) 0.80
Pressure effects on activity and stability of hyperthermophilic enzymes. Methods Enzymol (2001) 0.80
Size exclusion chromatography does not require pores. Proc Natl Acad Sci U S A (2000) 0.80
Callogenolytic activity of pigmented pseudomonads. Can J Microbiol (1970) 0.80
Adaptation of proteins from hyperthermophiles to high pressure and high temperature. J Mol Microbiol Biotechnol (1999) 0.80
Analysis of compression of polymer mushrooms using self-consistent field theory. J Chromatogr B Biomed Sci Appl (2000) 0.80
Macromolecular crowding and its consequences. Int Rev Cytol (2000) 0.79
Mechanisms of leukocyte infiltration of allografts. I. The separation and early appearance of two components. Surgery (1969) 0.79
Activation of enzymes for nonaqueous biocatalysis by denaturing concentrations of urea. Biochim Biophys Acta (2001) 0.79
Purification and functional characterization of a chaperone from Methanococcus jannaschii. Syst Appl Microbiol (1998) 0.79
Transition state stabilization of subtilisins in organic media. Biotechnol Bioeng (1994) 0.79
Use of a quantitative pediatric bleeding questionnaire to assess mucocutaneous bleeding symptoms in children with a platelet function disorder. J Thromb Haemost (2010) 0.79
Deactivation of alpha-chymotrypsin and alpha-chymotrypsin-CNBr-Sepharose 4B conjugates in aliphatic alcohols. Biochim Biophys Acta (1984) 0.78
Thermostability and irreversible activity loss of exoglucanase/xylanase Cex from Cellulomonas fimi. Biochemistry (1997) 0.78