Published in Acta Crystallogr D Biol Crystallogr on December 18, 2003
A general protocol for the generation of Nanobodies for structural biology. Nat Protoc (2014) 1.85
Structural basis for UTP specificity of RNA editing TUTases from Trypanosoma brucei. EMBO J (2005) 1.61
Biochemical and crystallographic analysis of substrate binding and conformational changes in acetyl-CoA synthetase. Biochemistry (2007) 1.49
Structure of PA1221, a nonribosomal peptide synthetase containing adenylation and peptidyl carrier protein domains. Biochemistry (2012) 1.19
The closed MTIP-myosin A-tail complex from the malaria parasite invasion machinery. J Mol Biol (2007) 1.15
Structural basis for antagonism of human interleukin 18 by poxvirus interleukin 18-binding protein. Proc Natl Acad Sci U S A (2008) 1.13
Structural and functional studies on the interaction of GspC and GspD in the type II secretion system. PLoS Pathog (2011) 1.12
Epigenetic epidemiology: promises for public health research. Environ Mol Mutagen (2014) 1.06
Structure of a Trypanosoma brucei alpha/beta-hydrolase fold protein with unknown function. Acta Crystallogr Sect F Struct Biol Cryst Commun (2008) 0.99
Structures of substrate- and inhibitor-bound adenosine deaminase from a human malaria parasite show a dramatic conformational change and shed light on drug selectivity. J Mol Biol (2008) 0.98
Crystallization screening: the influence of history on current practice. Acta Crystallogr F Struct Biol Commun (2014) 0.98
A CC' loop decoy peptide blocks the interaction between Act1 and IL-17RA to attenuate IL-17- and IL-25-induced inflammation. Sci Signal (2011) 0.98
Identification of the citrate-binding site of human ATP-citrate lyase using X-ray crystallography. J Biol Chem (2010) 0.90
Environment sensing and response mediated by ABC transporters. BMC Genomics (2011) 0.89
Energetic coupling between an oxidizable cysteine and the phosphorylatable N-terminus of human liver pyruvate kinase. Biochemistry (2013) 0.89
The 2.1 A crystal structure of an acyl-CoA synthetase from Methanosarcina acetivorans reveals an alternate acyl-binding pocket for small branched acyl substrates. Proteins (2009) 0.87
Monoolein lipid phases as incorporation and enrichment materials for membrane protein crystallization. PLoS One (2011) 0.85
Crystal structures and proposed structural/functional classification of three protozoan proteins from the isochorismatase superfamily. Protein Sci (2005) 0.85
Prediction of protein crystallization outcome using a hybrid method. J Struct Biol (2010) 0.82
Surface ligand effects on metal-affinity coordination to quantum dots: implications for nanoprobe self-assembly. Bioconjug Chem (2010) 0.82
Structure-function analysis of vaccinia virus H7 protein reveals a novel phosphoinositide binding fold essential for poxvirus replication. J Virol (2014) 0.80
Crystal structure of the pilotin from the enterohemorrhagic Escherichia coli type II secretion system. J Struct Biol (2013) 0.80
Functional and structural characterization of Vibrio cholerae extracellular serine protease B, VesB. J Biol Chem (2014) 0.80
Protein production for structural genomics using E. coli expression. Methods Mol Biol (2014) 0.80
Physical and functional interaction between the ID1 and p65 for activation of NF-κB. Am J Physiol Cell Physiol (2012) 0.80
ADP-Mg2+ bound to the ATP-grasp domain of ATP-citrate lyase. Acta Crystallogr Sect F Struct Biol Cryst Commun (2011) 0.79
Biochemical and structural characterization of the GTP-preferring succinyl-CoA synthetase from Thermus aquaticus. Acta Crystallogr D Biol Crystallogr (2012) 0.79
Structural and bioinformatic characterization of an Acinetobacter baumannii type II carrier protein. Acta Crystallogr D Biol Crystallogr (2014) 0.79
Structure of the unique SEFIR domain from human interleukin 17 receptor A reveals a composite ligand-binding site containing a conserved α-helix for Act1 binding and IL-17 signaling. Acta Crystallogr D Biol Crystallogr (2014) 0.78
The structure of a prophenoloxidase (PPO) from Anopheles gambiae provides new insights into the mechanism of PPO activation. BMC Biol (2016) 0.78
Structural basis for antagonizing a host restriction factor by C7 family of poxvirus host-range proteins. Proc Natl Acad Sci U S A (2015) 0.77
Structure of a Nudix hydrolase (MutT) in the Mg(2+)-bound state from Bartonella henselae, the bacterium responsible for cat scratch fever. Acta Crystallogr Sect F Struct Biol Cryst Commun (2011) 0.77
The detection and subsequent volume optimization of biological nanocrystals. Struct Dyn (2015) 0.77
The structure of Plasmodium vivax phosphatidylethanolamine-binding protein suggests a functional motif containing a left-handed helix. Acta Crystallogr Sect F Struct Biol Cryst Commun (2007) 0.76
Cellular and viral peptides bind multiple sites on the N-terminal domain of clathrin. Traffic (2016) 0.75
Screening a fragment cocktail library using ultrafiltration. Anal Bioanal Chem (2011) 0.75
Crystallization of interleukin-18 for structure-based inhibitor design. Acta Crystallogr F Struct Biol Commun (2015) 0.75
Two sensor kinases contribute to the hypoxic response of Mycobacterium tuberculosis. J Biol Chem (2004) 2.71
Type II secretion: from structure to function. FEMS Microbiol Lett (2006) 2.51
Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes. Mol Biochem Parasitol (2006) 2.45
Toxoplasma gondii calcium-dependent protein kinase 1 is a target for selective kinase inhibitors. Nat Struct Mol Biol (2010) 2.34
Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system. Nat Struct Mol Biol (2008) 2.19
The two GAF domains in phosphodiesterase 2A have distinct roles in dimerization and in cGMP binding. Proc Natl Acad Sci U S A (2002) 2.10
The type II secretion system: biogenesis, molecular architecture and mechanism. Nat Rev Microbiol (2012) 1.96
Specific Btk inhibition suppresses B cell- and myeloid cell-mediated arthritis. Nat Chem Biol (2010) 1.94
Crystal structure of the N-terminal domain of the secretin GspD from ETEC determined with the assistance of a nanobody. Structure (2009) 1.89
Sites of interaction between aldolase and thrombospondin-related anonymous protein in plasmodium. Mol Biol Cell (2003) 1.83
The 1.6-A crystal structure of the class of chaperones represented by Escherichia coli Hsp31 reveals a putative catalytic triad. Proc Natl Acad Sci U S A (2003) 1.72
High resolution crystal structure of a key editosome enzyme from Trypanosoma brucei: RNA editing ligase 1. J Mol Biol (2004) 1.67
Structural basis for the activation of cholera toxin by human ARF6-GTP. Science (2005) 1.66
Structural basis for UTP specificity of RNA editing TUTases from Trypanosoma brucei. EMBO J (2005) 1.61
Secretins: dynamic channels for protein transport across membranes. Trends Biochem Sci (2011) 1.58
Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae. J Mol Biol (2003) 1.55
The crystal structure of human tyrosyl-DNA phosphodiesterase, Tdp1. Structure (2002) 1.51
Crystal structure of a transition state mimic for Tdp1 assembled from vanadate, DNA, and a topoisomerase I-derived peptide. Chem Biol (2003) 1.47
Structure of the cholera toxin secretion channel in its closed state. Nat Struct Mol Biol (2010) 1.44
Insights into substrate binding and catalytic mechanism of human tyrosyl-DNA phosphodiesterase (Tdp1) from vanadate and tungstate-inhibited structures. J Mol Biol (2002) 1.37
Selective inhibitors of methionyl-tRNA synthetase have potent activity against Trypanosoma brucei Infection in Mice. Antimicrob Agents Chemother (2011) 1.37
Multiple determinants for selective inhibition of apicomplexan calcium-dependent protein kinase CDPK1. J Med Chem (2012) 1.35
Urea-based inhibitors of Trypanosoma brucei methionyl-tRNA synthetase: selectivity and in vivo characterization. J Med Chem (2012) 1.35
The X-ray structure of the type II secretion system complex formed by the N-terminal domain of EpsE and the cytoplasmic domain of EpsL of Vibrio cholerae. J Mol Biol (2005) 1.33
Structure of PolC reveals unique DNA binding and fidelity determinants. Proc Natl Acad Sci U S A (2008) 1.33
The power of vanadate in crystallographic investigations of phosphoryl transfer enzymes. FEBS Lett (2004) 1.32
Aldolase provides an unusual binding site for thrombospondin-related anonymous protein in the invasion machinery of the malaria parasite. Proc Natl Acad Sci U S A (2007) 1.31
GAF domains: two-billion-year-old molecular switches that bind cyclic nucleotides. Mol Interv (2002) 1.29
Structures of Mycobacterium tuberculosis DosR and DosR-DNA complex involved in gene activation during adaptation to hypoxic latency. J Mol Biol (2005) 1.26
Buffer optimization of thermal melt assays of Plasmodium proteins for detection of small-molecule ligands. J Biomol Screen (2009) 1.25
Characterization of Trypanosoma brucei dihydroorotate dehydrogenase as a possible drug target; structural, kinetic and RNAi studies. Mol Microbiol (2008) 1.22
Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids. EMBO J (2006) 1.21
Crystal structures of the response regulator DosR from Mycobacterium tuberculosis suggest a helix rearrangement mechanism for phosphorylation activation. J Mol Biol (2008) 1.20
Structural genomics of pathogenic protozoa: an overview. Methods Mol Biol (2008) 1.20
Distinct states of methionyl-tRNA synthetase indicate inhibitor binding by conformational selection. Structure (2012) 1.18
Crystal structure of a nitrate/nitrite exchanger. Nature (2013) 1.16
The closed MTIP-myosin A-tail complex from the malaria parasite invasion machinery. J Mol Biol (2007) 1.15
The structure of the cytoplasmic domain of EpsL, an inner membrane component of the type II secretion system of Vibrio cholerae: an unusual member of the actin-like ATPase superfamily. J Mol Biol (2004) 1.14
The structure of the transition state of the heterodimeric topoisomerase I of Leishmania donovani as a vanadate complex with nicked DNA. J Mol Biol (2006) 1.14
Structural and functional studies of EpsC, a crucial component of the type 2 secretion system from Vibrio cholerae. J Mol Biol (2006) 1.13
Structure of the Mycobacterium tuberculosis flavin dependent thymidylate synthase (MtbThyX) at 2.0A resolution. J Mol Biol (2005) 1.13
Structural and functional studies on the interaction of GspC and GspD in the type II secretion system. PLoS Pathog (2011) 1.12
Structure of the minor pseudopilin EpsH from the Type 2 secretion system of Vibrio cholerae. J Mol Biol (2007) 1.12
The role of medical structural genomics in discovering new drugs for infectious diseases. PLoS Comput Biol (2009) 1.11
Crystal structures of an intrinsically active cholera toxin mutant yield insight into the toxin activation mechanism. Biochemistry (2004) 1.09
Structure of the MTIP-MyoA complex, a key component of the malaria parasite invasion motor. Proc Natl Acad Sci U S A (2006) 1.09
Parasitology. Drugs to combat tropical protozoan parasites. Science (2002) 1.08
A protein-protein interaction map of trypanosome ~20S editosomes. J Biol Chem (2009) 1.08
Solution and crystallographic studies of branched multivalent ligands that inhibit the receptor-binding of cholera toxin. J Am Chem Soc (2002) 1.08
Explorations of peptide and oligonucleotide binding sites of tyrosyl-DNA phosphodiesterase using vanadate complexes. J Med Chem (2004) 1.07
The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the type 2 secretion system of Vibrio vulnificus. J Mol Biol (2007) 1.07
In vivo cross-linking of EpsG to EpsL suggests a role for EpsL as an ATPase-pseudopilin coupling protein in the Type II secretion system of Vibrio cholerae. Mol Microbiol (2011) 1.07
Calcium is essential for the major pseudopilin in the type 2 secretion system. J Biol Chem (2009) 1.05
Structures of enterococcal glycerol kinase in the absence and presence of glycerol: correlation of conformation to substrate binding and a mechanism of activation by phosphorylation. Biochemistry (2004) 1.05
The crystal structure of the periplasmic domain of the type II secretion system protein EpsM from Vibrio cholerae: the simplest version of the ferredoxin fold. J Mol Biol (2004) 1.04
Use of thermal melt curves to assess the quality of enzyme preparations. Anal Biochem (2009) 1.02
Biogenesis of peroxisomes and glycosomes: trypanosomatid glycosome assembly is a promising new drug target. FEMS Microbiol Rev (2004) 1.02
Crystal structure of an IdeR-DNA complex reveals a conformational change in activated IdeR for base-specific interactions. J Mol Biol (2004) 1.01
Functional studies of the Mycobacterium tuberculosis iron-dependent regulator. J Biol Chem (2004) 1.01
Characterization and crystal structure of a high-affinity pentavalent receptor-binding inhibitor for cholera toxin and E. coli heat-labile enterotoxin. J Am Chem Soc (2002) 1.00
Using fragment cocktail crystallography to assist inhibitor design of Trypanosoma brucei nucleoside 2-deoxyribosyltransferase. J Med Chem (2006) 1.00
Structure of a Trypanosoma brucei alpha/beta-hydrolase fold protein with unknown function. Acta Crystallogr Sect F Struct Biol Cryst Commun (2008) 0.99
Structures of substrate- and inhibitor-bound adenosine deaminase from a human malaria parasite show a dramatic conformational change and shed light on drug selectivity. J Mol Biol (2008) 0.98
Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs. J Mol Biol (2010) 0.97
Crystal structure of a heterodimer of editosome interaction proteins in complex with two copies of a cross-reacting nanobody. Nucleic Acids Res (2011) 0.97
Structural insights into the recognition of peroxisomal targeting signal 1 by Trypanosoma brucei peroxin 5. J Mol Biol (2008) 0.97
Effects of impurities on membrane-protein crystallization in different systems. Acta Crystallogr D Biol Crystallogr (2009) 0.97
Precursor structure of cephalosporin acylase. Insights into autoproteolytic activation in a new N-terminal hydrolase family. J Biol Chem (2001) 0.96
A new native EcHsp31 structure suggests a key role of structural flexibility for chaperone function. Protein Sci (2004) 0.96
Crystal structure of interleukin-6 in complex with a modified nucleic acid ligand. J Biol Chem (2014) 0.96
The binding of cholera toxin to the periplasmic vestibule of the type II secretion channel. Channels (Austin) (2011) 0.96
Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies. J Struct Biol (2010) 0.95
Crystal structures, metal activation, and DNA-binding properties of two-domain IdeR from Mycobacterium tuberculosis. Biochemistry (2007) 0.95
The role of lysine 532 in the catalytic mechanism of human topoisomerase I. J Biol Chem (2003) 0.95
Nonspanning bivalent ligands as improved surface receptor binding inhibitors of the cholera toxin B pentamer. Chem Biol (2004) 0.95
The dimer formed by the periplasmic domain of EpsL from the Type 2 Secretion System of Vibrio parahaemolyticus. J Struct Biol (2009) 0.94
Structure of Leishmania major methionyl-tRNA synthetase in complex with intermediate products methionyladenylate and pyrophosphate. Biochimie (2010) 0.93
Oligomerization of EpsE coordinates residues from multiple subunits to facilitate ATPase activity. J Biol Chem (2011) 0.93
Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major. Acta Crystallogr Sect F Struct Biol Cryst Commun (2006) 0.93
The linker-loop region of Escherichia coli chaperone Hsp31 functions as a gate that modulates high-affinity substrate binding at elevated temperatures. Proc Natl Acad Sci U S A (2004) 0.92
Crystals of peptide deformylase from Plasmodium falciparum reveal critical characteristics of the active site for drug design. Structure (2002) 0.92
Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins. Eur J Biochem (2003) 0.92
Leveraging structure determination with fragment screening for infectious disease drug targets: MECP synthase from Burkholderia pseudomallei. J Struct Funct Genomics (2011) 0.92
Crystal structure of GAP50, the anchor of the invasion machinery in the inner membrane complex of Plasmodium falciparum. J Struct Biol (2012) 0.92
Identifying regulators for EAG1 channels with a novel electrophysiology and tryptophan fluorescence based screen. PLoS One (2010) 0.91
The three-dimensional structure of the cytoplasmic domains of EpsF from the type 2 secretion system of Vibrio cholerae. J Struct Biol (2009) 0.91
3,5-Substituted phenyl galactosides as leads in designing effective cholera toxin antagonists; synthesis and crystallographic studies. Bioorg Med Chem (2004) 0.90
Structure determination of glycogen synthase kinase-3 from Leishmania major and comparative inhibitor structure-activity relationships with Trypanosoma brucei GSK-3. Mol Biochem Parasitol (2010) 0.90
Fragment-based cocktail crystallography by the medical structural genomics of pathogenic protozoa consortium. Curr Top Med Chem (2009) 0.90
NADP+ expels both the co-factor and a substrate analog from the Mycobacterium tuberculosis ThyX active site: opportunities for anti-bacterial drug design. J Mol Biol (2006) 0.89
Hexamers of the type II secretion ATPase GspE from Vibrio cholerae with increased ATPase activity. Structure (2013) 0.89