Published in J Mol Biol on October 24, 2003
Biogenesis, architecture, and function of bacterial type IV secretion systems. Annu Rev Microbiol (2005) 6.16
Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility. Structure (2007) 2.42
Type IV pili: paradoxes in form and function. Curr Opin Struct Biol (2008) 2.03
The type II secretion system: biogenesis, molecular architecture and mechanism. Nat Rev Microbiol (2012) 1.96
Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems. Biochim Biophys Acta (2004) 1.78
Hexameric structures of the archaeal secretion ATPase GspE and implications for a universal secretion mechanism. EMBO J (2007) 1.77
Distinct docking and stabilization steps of the Pseudopilus conformational transition path suggest rotational assembly of type IV pilus-like fibers. Structure (2014) 1.52
Disparate subcellular localization patterns of Pseudomonas aeruginosa Type IV pilus ATPases involved in twitching motility. J Bacteriol (2005) 1.47
PilB and PilT are ATPases acting antagonistically in type IV pilus function in Myxococcus xanthus. J Bacteriol (2008) 1.41
Structural and dynamic properties of bacterial type IV secretion systems (review). Mol Membr Biol (2005) 1.25
Molecular analysis of the Vibrio cholerae type II secretion ATPase EpsE. J Bacteriol (2005) 1.23
Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids. EMBO J (2006) 1.21
On the path to uncover the bacterial type II secretion system. Philos Trans R Soc Lond B Biol Sci (2012) 1.15
Type IV pili in Gram-positive bacteria. Microbiol Mol Biol Rev (2013) 1.14
XpsE oligomerization triggered by ATP binding, not hydrolysis, leads to its association with XpsL. EMBO J (2006) 1.12
Structure of the minor pseudopilin EpsH from the Type 2 secretion system of Vibrio cholerae. J Mol Biol (2007) 1.12
Protein Secretion Systems in Pseudomonas aeruginosa: An Essay on Diversity, Evolution, and Function. Front Microbiol (2011) 1.12
P. aeruginosa PilT structures with and without nucleotide reveal a dynamic type IV pilus retraction motor. J Mol Biol (2010) 1.12
Periplasmic protein-protein contacts in the inner membrane protein Wzc form a tetrameric complex required for the assembly of Escherichia coli group 1 capsules. J Biol Chem (2005) 1.11
Insights into FlaI functions in archaeal motor assembly and motility from structures, conformations, and genetics. Mol Cell (2013) 1.09
The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the type 2 secretion system of Vibrio vulnificus. J Mol Biol (2007) 1.07
In vivo cross-linking of EpsG to EpsL suggests a role for EpsL as an ATPase-pseudopilin coupling protein in the Type II secretion system of Vibrio cholerae. Mol Microbiol (2011) 1.07
Structural insights into the Type II secretion nanomachine. Curr Opin Struct Biol (2012) 1.06
Functional dissection of a conserved motif within the pilus retraction protein PilT. J Bacteriol (2005) 1.04
Membrane association and multimerization of TcpT, the cognate ATPase ortholog of the Vibrio cholerae toxin-coregulated-pilus biogenesis apparatus. J Bacteriol (2007) 0.97
The dimer formed by the periplasmic domain of EpsL from the Type 2 Secretion System of Vibrio parahaemolyticus. J Struct Biol (2009) 0.94
Oligomerization of EpsE coordinates residues from multiple subunits to facilitate ATPase activity. J Biol Chem (2011) 0.93
The three-dimensional structure of the cytoplasmic domains of EpsF from the type 2 secretion system of Vibrio cholerae. J Struct Biol (2009) 0.91
Identification and characterization of a unique, zinc-containing transport ATPase essential for natural transformation in Thermus thermophilus HB27. Extremophiles (2011) 0.90
Hexamers of the type II secretion ATPase GspE from Vibrio cholerae with increased ATPase activity. Structure (2013) 0.89
Cysteine scanning mutagenesis and disulfide mapping analysis of arrangement of GspC and GspD protomers within the type 2 secretion system. J Biol Chem (2012) 0.89
Systematic Identification of Cyclic-di-GMP Binding Proteins in Vibrio cholerae Reveals a Novel Class of Cyclic-di-GMP-Binding ATPases Associated with Type II Secretion Systems. PLoS Pathog (2015) 0.88
Structure and mechanism of the PilF DNA transformation ATPase from Thermus thermophilus. Biochem J (2013) 0.88
Regulation of the type IV secretion ATPase TrwD by magnesium: implications for catalytic mechanism of the secretion ATPase superfamily. J Biol Chem (2012) 0.87
Functional analysis of PilT from the toxic cyanobacterium Microcystis aeruginosa PCC 7806. J Bacteriol (2006) 0.86
The secretion ATPase ComGA is required for the binding and transport of transforming DNA. Mol Microbiol (2011) 0.84
Crystal structure of the full-length ATPase GspE from the Vibrio vulnificus type II secretion system in complex with the cytoplasmic domain of GspL. J Struct Biol (2014) 0.82
Functional characterization of EpsC, a component of the type II secretion system, in the pathogenicity of Vibrio vulnificus. Infect Immun (2011) 0.81
The Type IV Pilus Assembly ATPase PilB of Myxococcus xanthus Interacts with the Inner Membrane Platform Protein PilC and the Nucleotide-binding Protein PilM. J Biol Chem (2016) 0.81
Zinc and ATP binding of the hexameric AAA-ATPase PilF from Thermus thermophilus: role in complex stability, piliation, adhesion, twitching motility, and natural transformation. J Biol Chem (2014) 0.81
Type IV pilus biogenesis, twitching motility, and DNA uptake in Thermus thermophilus: discrete roles of antagonistic ATPases PilF, PilT1, and PilT2. Appl Environ Microbiol (2013) 0.79
Zinc coordination is essential for the function and activity of the type II secretion ATPase EpsE. Microbiologyopen (2016) 0.76
Cyclic Di-GMP Binding by an Assembly ATPase (PilB2) and Control of Type IV Pilin Polymerization in the Gram-Positive Pathogen Clostridium perfringens. J Bacteriol (2017) 0.75
Appendages of the cyanobacterial cell. Life (Basel) (2015) 0.75
Construction of a Vibrio cholerae prototype vaccine strain O395-N1-E1 which accumulates cell-associated cholera toxin B subunit. Vaccine (2008) 0.75
Characterization and localization of the Campylobacter jejuni transformation system proteins CtsE, CtsP, and CtsX. J Bacteriol (2014) 0.75
Tissue-type plasminogen activator induces opening of the blood-brain barrier via the LDL receptor-related protein. J Clin Invest (2003) 3.41
Two sensor kinases contribute to the hypoxic response of Mycobacterium tuberculosis. J Biol Chem (2004) 2.71
Type II secretion: from structure to function. FEMS Microbiol Lett (2006) 2.51
Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes. Mol Biochem Parasitol (2006) 2.45
Toxoplasma gondii calcium-dependent protein kinase 1 is a target for selective kinase inhibitors. Nat Struct Mol Biol (2010) 2.34
Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system. Nat Struct Mol Biol (2008) 2.19
The two GAF domains in phosphodiesterase 2A have distinct roles in dimerization and in cGMP binding. Proc Natl Acad Sci U S A (2002) 2.10
The type II secretion system: biogenesis, molecular architecture and mechanism. Nat Rev Microbiol (2012) 1.96
Crystal structure of the N-terminal domain of the secretin GspD from ETEC determined with the assistance of a nanobody. Structure (2009) 1.89
Sites of interaction between aldolase and thrombospondin-related anonymous protein in plasmodium. Mol Biol Cell (2003) 1.83
The 1.6-A crystal structure of the class of chaperones represented by Escherichia coli Hsp31 reveals a putative catalytic triad. Proc Natl Acad Sci U S A (2003) 1.72
High resolution crystal structure of a key editosome enzyme from Trypanosoma brucei: RNA editing ligase 1. J Mol Biol (2004) 1.67
Structural basis for the activation of cholera toxin by human ARF6-GTP. Science (2005) 1.66
Structural basis for UTP specificity of RNA editing TUTases from Trypanosoma brucei. EMBO J (2005) 1.61
Secretins: dynamic channels for protein transport across membranes. Trends Biochem Sci (2011) 1.58
The crystal structure of human tyrosyl-DNA phosphodiesterase, Tdp1. Structure (2002) 1.51
An improved protocol for rapid freezing of protein samples for long-term storage. Acta Crystallogr D Biol Crystallogr (2003) 1.49
Crystal structure of a transition state mimic for Tdp1 assembled from vanadate, DNA, and a topoisomerase I-derived peptide. Chem Biol (2003) 1.47
Regulation of seizure spreading by neuroserpin and tissue-type plasminogen activator is plasminogen-independent. J Clin Invest (2002) 1.44
Structure of the cholera toxin secretion channel in its closed state. Nat Struct Mol Biol (2010) 1.44
Insights into substrate binding and catalytic mechanism of human tyrosyl-DNA phosphodiesterase (Tdp1) from vanadate and tungstate-inhibited structures. J Mol Biol (2002) 1.37
Selective inhibitors of methionyl-tRNA synthetase have potent activity against Trypanosoma brucei Infection in Mice. Antimicrob Agents Chemother (2011) 1.37
Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria. Biochem Biophys Res Commun (2011) 1.35
Multiple determinants for selective inhibition of apicomplexan calcium-dependent protein kinase CDPK1. J Med Chem (2012) 1.35
Urea-based inhibitors of Trypanosoma brucei methionyl-tRNA synthetase: selectivity and in vivo characterization. J Med Chem (2012) 1.35
The X-ray structure of the type II secretion system complex formed by the N-terminal domain of EpsE and the cytoplasmic domain of EpsL of Vibrio cholerae. J Mol Biol (2005) 1.33
The power of vanadate in crystallographic investigations of phosphoryl transfer enzymes. FEBS Lett (2004) 1.32
Aldolase provides an unusual binding site for thrombospondin-related anonymous protein in the invasion machinery of the malaria parasite. Proc Natl Acad Sci U S A (2007) 1.31
GAF domains: two-billion-year-old molecular switches that bind cyclic nucleotides. Mol Interv (2002) 1.29
Structures of Mycobacterium tuberculosis DosR and DosR-DNA complex involved in gene activation during adaptation to hypoxic latency. J Mol Biol (2005) 1.26
Buffer optimization of thermal melt assays of Plasmodium proteins for detection of small-molecule ligands. J Biomol Screen (2009) 1.25
Molecular analysis of the Vibrio cholerae type II secretion ATPase EpsE. J Bacteriol (2005) 1.23
Characterization of Trypanosoma brucei dihydroorotate dehydrogenase as a possible drug target; structural, kinetic and RNAi studies. Mol Microbiol (2008) 1.22
Compromised outer membrane integrity in Vibrio cholerae Type II secretion mutants. J Bacteriol (2007) 1.21
Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids. EMBO J (2006) 1.21
Crystal structures of the response regulator DosR from Mycobacterium tuberculosis suggest a helix rearrangement mechanism for phosphorylation activation. J Mol Biol (2008) 1.20
Proteomic analysis of the Vibrio cholerae type II secretome reveals new proteins, including three related serine proteases. J Biol Chem (2011) 1.20
Structural genomics of pathogenic protozoa: an overview. Methods Mol Biol (2008) 1.20
Distinct states of methionyl-tRNA synthetase indicate inhibitor binding by conformational selection. Structure (2012) 1.18
Docking and assembly of the type II secretion complex of Vibrio cholerae. J Bacteriol (2009) 1.16
The closed MTIP-myosin A-tail complex from the malaria parasite invasion machinery. J Mol Biol (2007) 1.15
The structure of the transition state of the heterodimeric topoisomerase I of Leishmania donovani as a vanadate complex with nicked DNA. J Mol Biol (2006) 1.14
The structure of the cytoplasmic domain of EpsL, an inner membrane component of the type II secretion system of Vibrio cholerae: an unusual member of the actin-like ATPase superfamily. J Mol Biol (2004) 1.14
Structural and functional studies of EpsC, a crucial component of the type 2 secretion system from Vibrio cholerae. J Mol Biol (2006) 1.13
Structure of the Mycobacterium tuberculosis flavin dependent thymidylate synthase (MtbThyX) at 2.0A resolution. J Mol Biol (2005) 1.13
Structure of the minor pseudopilin EpsH from the Type 2 secretion system of Vibrio cholerae. J Mol Biol (2007) 1.12
Structural and functional studies on the interaction of GspC and GspD in the type II secretion system. PLoS Pathog (2011) 1.12
The role of medical structural genomics in discovering new drugs for infectious diseases. PLoS Comput Biol (2009) 1.11
Crystal structures of an intrinsically active cholera toxin mutant yield insight into the toxin activation mechanism. Biochemistry (2004) 1.09
Structure of the MTIP-MyoA complex, a key component of the malaria parasite invasion motor. Proc Natl Acad Sci U S A (2006) 1.09
Parasitology. Drugs to combat tropical protozoan parasites. Science (2002) 1.08
A protein-protein interaction map of trypanosome ~20S editosomes. J Biol Chem (2009) 1.08
Solution and crystallographic studies of branched multivalent ligands that inhibit the receptor-binding of cholera toxin. J Am Chem Soc (2002) 1.08
Explorations of peptide and oligonucleotide binding sites of tyrosyl-DNA phosphodiesterase using vanadate complexes. J Med Chem (2004) 1.07
In vivo cross-linking of EpsG to EpsL suggests a role for EpsL as an ATPase-pseudopilin coupling protein in the Type II secretion system of Vibrio cholerae. Mol Microbiol (2011) 1.07
The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the type 2 secretion system of Vibrio vulnificus. J Mol Biol (2007) 1.07
Calcium is essential for the major pseudopilin in the type 2 secretion system. J Biol Chem (2009) 1.05
Structures of enterococcal glycerol kinase in the absence and presence of glycerol: correlation of conformation to substrate binding and a mechanism of activation by phosphorylation. Biochemistry (2004) 1.05
The crystal structure of the periplasmic domain of the type II secretion system protein EpsM from Vibrio cholerae: the simplest version of the ferredoxin fold. J Mol Biol (2004) 1.04
Use of thermal melt curves to assess the quality of enzyme preparations. Anal Biochem (2009) 1.02
Biogenesis of peroxisomes and glycosomes: trypanosomatid glycosome assembly is a promising new drug target. FEMS Microbiol Rev (2004) 1.02
Cell envelope perturbation induces oxidative stress and changes in iron homeostasis in Vibrio cholerae. J Bacteriol (2009) 1.01
Functional studies of the Mycobacterium tuberculosis iron-dependent regulator. J Biol Chem (2004) 1.01
Crystal structure of an IdeR-DNA complex reveals a conformational change in activated IdeR for base-specific interactions. J Mol Biol (2004) 1.01
Characterization and crystal structure of a high-affinity pentavalent receptor-binding inhibitor for cholera toxin and E. coli heat-labile enterotoxin. J Am Chem Soc (2002) 1.00
Using fragment cocktail crystallography to assist inhibitor design of Trypanosoma brucei nucleoside 2-deoxyribosyltransferase. J Med Chem (2006) 1.00
Structure of a Trypanosoma brucei alpha/beta-hydrolase fold protein with unknown function. Acta Crystallogr Sect F Struct Biol Cryst Commun (2008) 0.99
Structures of substrate- and inhibitor-bound adenosine deaminase from a human malaria parasite show a dramatic conformational change and shed light on drug selectivity. J Mol Biol (2008) 0.98
Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs. J Mol Biol (2010) 0.97
Crystal structure of a heterodimer of editosome interaction proteins in complex with two copies of a cross-reacting nanobody. Nucleic Acids Res (2011) 0.97
Structural insights into the recognition of peroxisomal targeting signal 1 by Trypanosoma brucei peroxin 5. J Mol Biol (2008) 0.97
Precursor structure of cephalosporin acylase. Insights into autoproteolytic activation in a new N-terminal hydrolase family. J Biol Chem (2001) 0.96
The binding of cholera toxin to the periplasmic vestibule of the type II secretion channel. Channels (Austin) (2011) 0.96
A new native EcHsp31 structure suggests a key role of structural flexibility for chaperone function. Protein Sci (2004) 0.96
Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies. J Struct Biol (2010) 0.95
Crystal structures, metal activation, and DNA-binding properties of two-domain IdeR from Mycobacterium tuberculosis. Biochemistry (2007) 0.95
Nonspanning bivalent ligands as improved surface receptor binding inhibitors of the cholera toxin B pentamer. Chem Biol (2004) 0.95
The role of lysine 532 in the catalytic mechanism of human topoisomerase I. J Biol Chem (2003) 0.95
The dimer formed by the periplasmic domain of EpsL from the Type 2 Secretion System of Vibrio parahaemolyticus. J Struct Biol (2009) 0.94
Structure of Leishmania major methionyl-tRNA synthetase in complex with intermediate products methionyladenylate and pyrophosphate. Biochimie (2010) 0.93
Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major. Acta Crystallogr Sect F Struct Biol Cryst Commun (2006) 0.93
Oligomerization of EpsE coordinates residues from multiple subunits to facilitate ATPase activity. J Biol Chem (2011) 0.93
The linker-loop region of Escherichia coli chaperone Hsp31 functions as a gate that modulates high-affinity substrate binding at elevated temperatures. Proc Natl Acad Sci U S A (2004) 0.92
Crystals of peptide deformylase from Plasmodium falciparum reveal critical characteristics of the active site for drug design. Structure (2002) 0.92
Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins. Eur J Biochem (2003) 0.92
Crystal structure of GAP50, the anchor of the invasion machinery in the inner membrane complex of Plasmodium falciparum. J Struct Biol (2012) 0.92
The three-dimensional structure of the cytoplasmic domains of EpsF from the type 2 secretion system of Vibrio cholerae. J Struct Biol (2009) 0.91
Structure determination of glycogen synthase kinase-3 from Leishmania major and comparative inhibitor structure-activity relationships with Trypanosoma brucei GSK-3. Mol Biochem Parasitol (2010) 0.90
Mapping critical interactive sites within the periplasmic domain of the Vibrio cholerae type II secretion protein EpsM. J Bacteriol (2007) 0.90