Published in Anal Biochem on February 01, 2004
Coordinated effects of distal mutations on environmentally coupled tunneling in dihydrofolate reductase. Proc Natl Acad Sci U S A (2006) 2.37
Effects of a distal mutation on active site chemistry. Biochemistry (2006) 1.59
The role of enzyme dynamics and tunnelling in catalysing hydride transfer: studies of distal mutants of dihydrofolate reductase. Philos Trans R Soc Lond B Biol Sci (2006) 1.39
Effects of the donor-acceptor distance and dynamics on hydride tunneling in the dihydrofolate reductase catalyzed reaction. J Am Chem Soc (2012) 1.37
Extension and limits of the network of coupled motions correlated to hydride transfer in dihydrofolate reductase. J Am Chem Soc (2014) 1.14
Triple isotopic labeling and kinetic isotope effects: exposing H-transfer steps in enzymatic systems. Biochemistry (2011) 1.01
Preservation of protein dynamics in dihydrofolate reductase evolution. J Biol Chem (2013) 1.01
Escherichia coli dihydrofolate reductase catalyzed proton and hydride transfers: temporal order and the roles of Asp27 and Tyr100. Proc Natl Acad Sci U S A (2014) 0.90
Determination of concentration and activity of immobilized enzymes. Anal Biochem (2015) 0.81
Network of remote and local protein dynamics in dihydrofolate reductase catalysis. ACS Catal (2015) 0.79
Engineering the respiratory complex I to energy-converting NADPH:ubiquinone oxidoreductase. J Biol Chem (2011) 0.79
Synthesis of radiolabeled nicotinamide cofactors from labeled pyridines: versatile probes for enzyme kinetics. Anal Biochem (2012) 0.76
The Effect of Protein Mass Modulation on Human Dihydrofolate Reductase. Biochemistry (2016) 0.76
Coordinated effects of distal mutations on environmentally coupled tunneling in dihydrofolate reductase. Proc Natl Acad Sci U S A (2006) 2.37
Tunneling and coupled motion in the Escherichia coli dihydrofolate reductase catalysis. J Am Chem Soc (2004) 2.21
Hydrogen tunneling links protein dynamics to enzyme catalysis. Annu Rev Biochem (2013) 1.76
An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene. Nature (2009) 1.62
Effects of a distal mutation on active site chemistry. Biochemistry (2006) 1.59
Mechanistic studies of a flavin-dependent thymidylate synthase. Biochemistry (2004) 1.55
Vibrationally enhanced hydrogen tunneling in the Escherichia coli thymidylate synthase catalyzed reaction. Biochemistry (2004) 1.49
The role of enzyme dynamics and tunnelling in catalysing hydride transfer: studies of distal mutants of dihydrofolate reductase. Philos Trans R Soc Lond B Biol Sci (2006) 1.39
Effects of the donor-acceptor distance and dynamics on hydride tunneling in the dihydrofolate reductase catalyzed reaction. J Am Chem Soc (2012) 1.37
Hydrogen donor-acceptor fluctuations from kinetic isotope effects: a phenomenological model. Biochemistry (2012) 1.28
Role of Y94 in proton and hydride transfers catalyzed by thymidylate synthase. Biochemistry (2007) 1.26
Temperature dependence of the kinetic isotope effects in thymidylate synthase. A theoretical study. J Am Chem Soc (2011) 1.21
Synthesis of R and S tritiated reduced beta-nicotinamide adenine dinucleotide 2' phosphate. Anal Biochem (2004) 1.19
Purification, analysis, and preservation of reduced nicotinamide adenine dinucleotide 2'-phosphate. Anal Biochem (2003) 1.19
Elusive transition state of alcohol dehydrogenase unveiled. Proc Natl Acad Sci U S A (2010) 1.17
Fast enzyme dynamics at the active site of formate dehydrogenase. J Am Chem Soc (2007) 1.16
Extension and limits of the network of coupled motions correlated to hydride transfer in dihydrofolate reductase. J Am Chem Soc (2014) 1.14
Nonperfect synchronization of reaction center rehybridization in the transition state of the hydride transfer catalyzed by dihydrofolate reductase. J Am Chem Soc (2005) 1.12
Characterizing the dynamics of functionally relevant complexes of formate dehydrogenase. Proc Natl Acad Sci U S A (2010) 1.11
A quantum mechanics/molecular mechanics study of the catalytic mechanism of the thymidylate synthase. Biochemistry (2007) 1.09
A remote mutation affects the hydride transfer by disrupting concerted protein motions in thymidylate synthase. J Am Chem Soc (2012) 1.08
Thymidylate synthase catalyzed H-transfers: two chapters in one tale. J Am Chem Soc (2010) 1.08
Thymidyl biosynthesis enzymes as antibiotic targets. Appl Microbiol Biotechnol (2007) 1.07
Tuning of the H-transfer coordinate in primitive versus well-evolved enzymes. Chemphyschem (2008) 1.07
Microscale synthesis of 2-tritiated isopropanol and 4R-tritiated reduced nicotinamide adenine dinucleotide phosphate. Anal Biochem (2003) 1.05
Examination of enzymatic H-tunneling through kinetics and dynamics. J Am Chem Soc (2009) 1.05
Protein mass-modulated effects in the catalytic mechanism of dihydrofolate reductase: beyond promoting vibrations. J Am Chem Soc (2014) 1.04
Arrhenius curves of hydrogen transfers: tunnel effects, isotope effects and effects of pre-equilibria. Philos Trans R Soc Lond B Biol Sci (2006) 1.03
Isotope effects as probes for enzyme catalyzed hydrogen-transfer reactions. Molecules (2013) 1.03
Triple isotopic labeling and kinetic isotope effects: exposing H-transfer steps in enzymatic systems. Biochemistry (2011) 1.01
QM/MM study of thymidylate synthase: enzymatic motions and the temperature dependence of the rate limiting step. J Phys Chem A (2009) 1.01
Trapping of an intermediate in the reaction catalyzed by flavin-dependent thymidylate synthase. J Am Chem Soc (2012) 1.01
Preservation of protein dynamics in dihydrofolate reductase evolution. J Biol Chem (2013) 1.01
Microscale synthesis of isotopically labeled R-[6-xH]N5,N10-methylene-5,6,7,8-tetrahydrofolate as a cofactor for thymidylate synthase. Anal Biochem (2004) 0.97
Hydride transfer versus hydrogen radical transfer in thymidylate synthase. J Am Chem Soc (2006) 0.96
QM/MM calculations suggest a novel intermediate following the proton abstraction catalyzed by thymidylate synthase. Biochemistry (2013) 0.95
The effect of active-site isoleucine to alanine mutation on the DHFR catalyzed hydride-transfer. Chem Commun (Camb) (2010) 0.93
The relationships between oxidase and synthase activities of flavin dependent thymidylate synthase (FDTS). Chem Commun (Camb) (2007) 0.92
Folate binding site of flavin-dependent thymidylate synthase. Proc Natl Acad Sci U S A (2012) 0.92
A critical test of the "tunneling and coupled motion" concept in enzymatic alcohol oxidation. J Am Chem Soc (2013) 0.92
Experimental and theoretical studies of enzyme-catalyzed hydrogen-transfer reactions. Adv Protein Chem Struct Biol (2012) 0.91
Mg2+ binds to the surface of thymidylate synthase and affects hydride transfer at the interior active site. J Am Chem Soc (2013) 0.89
Concerted versus stepwise mechanism in thymidylate synthase. J Am Chem Soc (2014) 0.89
3-picolyl azide adenine dinucleotide as a probe of femtosecond to picosecond enzyme dynamics. J Phys Chem B (2011) 0.88
Flavin-dependent thymidylate synthase: a novel pathway towards thymine. Arch Biochem Biophys (2009) 0.88
Oxidase activity of a flavin-dependent thymidylate synthase. FEBS J (2009) 0.88
Exploring the molecular origins of protein dynamics in the active site of human carbonic anhydrase II. J Phys Chem B (2009) 0.88
Mechanisms and inhibition of uracil methylating enzymes. Bioorg Chem (2011) 0.87
Characterization of azido-NAD+ to assess its potential as a two-dimensional infrared probe of enzyme dynamics. Anal Biochem (2010) 0.87
A lag-phase in the reduction of flavin dependent thymidylate synthase (FDTS) revealed a mechanistic missing link. Chem Commun (Camb) (2006) 0.87
Two-dimensional infrared spectroscopy of azido-nicotinamide adenine dinucleotide in water. J Chem Phys (2011) 0.85
Relationship of femtosecond-picosecond dynamics to enzyme-catalyzed H-transfer. Top Curr Chem (2013) 0.85
Substrate activation in flavin-dependent thymidylate synthase. J Am Chem Soc (2014) 0.85
Comparative hydrogen-deuterium exchange for a mesophilic vs thermophilic dihydrofolate reductase at 25 °C: identification of a single active site region with enhanced flexibility in the mesophilic protein. Biochemistry (2011) 0.84
Novel positron emission tomography tracer distinguishes normal from cancerous cells. J Biol Chem (2011) 0.83
Simulations of remote mutants of dihydrofolate reductase reveal the nature of a network of residues coupled to hydride transfer. J Comput Chem (2014) 0.81
Self-assembled enzymatic monolayer directly bound to a gold surface: activity and molecular recognition force spectroscopy studies. J Am Chem Soc (2011) 0.80
Competitive 15N kinetic isotope effects of nitrogenase-catalyzed dinitrogen reduction. J Am Chem Soc (2004) 0.78
Free energy simulations of active-site mutants of dihydrofolate reductase. J Phys Chem B (2014) 0.77
A fast chemoenzymatic synthesis of [11C]-N5,N10-methylenetetrahydrofolate as a potential PET tracer for proliferating cells. Nucl Med Biol (2012) 0.77
A model reaction assesses contribution of H-tunneling and coupled motions to enzyme catalysis. J Org Chem (2012) 0.76
Synthesis of radiolabeled nicotinamide cofactors from labeled pyridines: versatile probes for enzyme kinetics. Anal Biochem (2012) 0.76
The effect of electrostatic shielding on H tunneling in R67 dihydrofolate reductase. Chembiochem (2009) 0.76
Stereospecific multiple isotopic labeling of benzyl alcohol. J Labelled Comp Radiopharm (2013) 0.75
Targeting the de novo biosynthesis of thymidylate for the development of a PET probe for pancreatic cancer imaging. Biochemistry (2015) 0.75
Protein Mass Effects on Formate Dehydrogenase. J Am Chem Soc (2017) 0.75