Rapid and efficient polymerase chain reaction-based strategies for one-site and two-site mutagenesis.

PubWeight™: 0.83‹?›

🔗 View Article (PMID 15265750)

Published in Anal Biochem on August 15, 2004

Authors

Zhilong Gong1, Hai Zhang, Stephan Gabos, Xing-Fang Li

Author Affiliations

1: Environmental Health Sciences, Department of Public Health Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2G3.

Articles by these authors

Hypothalamic IKKbeta/NF-kappaB and ER stress link overnutrition to energy imbalance and obesity. Cell (2008) 6.83

BIPES, a cost-effective high-throughput method for assessing microbial diversity. ISME J (2010) 4.52

Hypothalamic programming of systemic ageing involving IKK-β, NF-κB and GnRH. Nature (2013) 3.22

Dynamic monitoring of cytotoxicity on microelectronic sensors. Chem Res Toxicol (2005) 2.08

L1/2 regularization: a thresholding representation theory and a fast solver. IEEE Trans Neural Netw Learn Syst (2012) 1.87

SIRT1 overexpression antagonizes cellular senescence with activated ERK/S6k1 signaling in human diploid fibroblasts. PLoS One (2008) 1.58

Characterization of new nitrosamines in drinking water using liquid chromatography tandem mass spectrometry. Environ Sci Technol (2006) 1.55

Neural dysregulation of peripheral insulin action and blood pressure by brain endoplasmic reticulum stress. Proc Natl Acad Sci U S A (2011) 1.52

Arsenic on the hands of children after playing in playgrounds. Environ Health Perspect (2004) 1.52

Neuropeptide exocytosis involving synaptotagmin-4 and oxytocin in hypothalamic programming of body weight and energy balance. Neuron (2011) 1.45

Zinc finger protein ZBTB20 is a key repressor of alpha-fetoprotein gene transcription in liver. Proc Natl Acad Sci U S A (2008) 1.38

Ultrasensitive assays for proteins. Analyst (2007) 1.35

Arsenic binding to proteins. Chem Rev (2013) 1.28

miR-101 inhibits autophagy and enhances cisplatin-induced apoptosis in hepatocellular carcinoma cells. Oncol Rep (2013) 1.18

Sparse logistic regression with a L1/2 penalty for gene selection in cancer classification. BMC Bioinformatics (2013) 1.16

Two-stage clustering (TSC): a pipeline for selecting operational taxonomic units for the high-throughput sequencing of PCR amplicons. PLoS One (2012) 1.15

Microelectronic cell sensor assay for detection of cytotoxicity and prediction of acute toxicity. Toxicol In Vitro (2006) 1.08

Zinc finger protein Zbtb20 is essential for postnatal survival and glucose homeostasis. Mol Cell Biol (2009) 1.07

MicroRNA regulation of STAT4 protein expression: rapid and sensitive modulation of IL-12 signaling in human natural killer cells. Blood (2011) 1.03

Analysis of independent microarray datasets of renal biopsies identifies a robust transcript signature of acute allograft rejection. Transpl Int (2008) 1.01

DNA-mediated homogeneous binding assays for nucleic acids and proteins. Chem Rev (2012) 1.01

Detection of viable but nonculturable Escherichia coli O157:H7 bacteria in drinking water and river water. Appl Environ Microbiol (2008) 0.99

Evidence of hemoglobin binding to arsenic as a basis for the accumulation of arsenic in rat blood. Chem Res Toxicol (2004) 0.99

Selection of aptamers against live bacterial cells. Anal Chem (2008) 0.98

Interaction of trivalent arsenicals with metallothionein. Chem Res Toxicol (2003) 0.98

Scanning electron microscopic evaluation of debris and smear layer remaining following use of ProTaper and Hero Shaper instruments in combination with NaOCl and EDTA irrigation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod (2008) 0.95

Effects of mineral trioxide aggregate on human dental pulp cells after pulp-capping procedures. J Endod (2010) 0.95

Potential factors that may affect acceptance of routine prenatal HIV testing. Can J Public Health (2005) 0.95

Exposure-response analysis and risk assessment for lung cancer in relationship to silica exposure: a 44-year cohort study of 34,018 workers. Am J Epidemiol (2013) 0.95

Modulation of Ca2+ signals by phosphatidylinositol-linked novel D1 dopamine receptor in hippocampal neurons. J Neurochem (2006) 0.94

Arsenic Species in Chicken Breast: Temporal Variations of Metabolites, Elimination Kinetics, and Residual Concentrations. Environ Health Perspect (2016) 0.93

Functional interaction between cellular p100 and the dengue virus 3' UTR. J Gen Virol (2010) 0.93

A multi-function public health surveillance system and the lessons learned in its development: the Alberta Real Time Syndromic Surveillance Net. Can J Public Health (2011) 0.93

The zinc finger protein ZBTB20 regulates transcription of fructose-1,6-bisphosphatase 1 and β cell function in mice. Gastroenterology (2012) 0.92

Characterization and determination of chloro- and bromo-benzoquinones as new chlorination disinfection byproducts in drinking water. Anal Chem (2010) 0.91

A cell-microelectronic sensing technique for profiling cytotoxicity of chemicals. Anal Chim Acta (2008) 0.91

Formation of N-nitrosamines from eleven disinfection treatments of seven different surface waters. Environ Sci Technol (2008) 0.91

Proliferation-attenuating and apoptosis-inducing effects of tryptanthrin on human chronic myeloid leukemia K562 cell line in vitro. Int J Mol Sci (2011) 0.91

Effect of surface treatments and cyclic loading on the bond strength of acrylic resin denture teeth with autopolymerized repair acrylic resin. J Prosthet Dent (2010) 0.91

Focal adhesion kinase: important to prostaglandin E2-mediated adhesion, migration and invasion in hepatocellular carcinoma cells. Oncol Rep (2009) 0.91

Rapid separation and identification of phenolic and diterpenoid constituents from Radix Salvia miltiorrhizae by high-performance liquid chromatography diode-array detection, electrospray ionization time-of-flight mass spectrometry and electrospray ionization quadrupole ion trap mass spectrometry. Rapid Commun Mass Spectrom (2007) 0.91

Use of quantum dots in the development of assays for cancer biomarkers. Anal Bioanal Chem (2010) 0.90

DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Anal Chem (2011) 0.90

Shp2 controls female body weight and energy balance by integrating leptin and estrogen signals. Mol Cell Biol (2012) 0.90

Synthesis of dihydrofuroaporphine derivatives: identification of a potent and selective serotonin 5-HT 1A receptor agonist. J Med Chem (2010) 0.90

Phosphate: known and potential roles during development and regeneration of teeth and supporting structures. Birth Defects Res C Embryo Today (2008) 0.90

Dynamic DNA assemblies mediated by binding-induced DNA strand displacement. J Am Chem Soc (2013) 0.90

Assembling DNA through affinity binding to achieve ultrasensitive protein detection. Angew Chem Int Ed Engl (2013) 0.89

Ultrasensitive detection of proteins by amplification of affinity aptamers. Angew Chem Int Ed Engl (2006) 0.89

Aptamer-based affinity chromatographic assays for thrombin. Anal Chem (2008) 0.89

Binding of dimethylarsinous acid to cys-13alpha of rat hemoglobin is responsible for the retention of arsenic in rat blood. Chem Res Toxicol (2007) 0.88

Prostaglandin E2 accelerates invasion by upregulating Snail in hepatocellular carcinoma cells. Tumour Biol (2014) 0.88

Reverse transcription-multiplex PCR assay for simultaneous detection of Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi. Clin Chem (2004) 0.88

Comparative pharmacokinetics of prim-O-glucosylcimifugin and cimifugin by liquid chromatography-mass spectrometry after oral administration of Radix Saposhnikoviae extract, cimifugin monomer solution and prim-O-glucosylcimifugin monomer solution to rats. Biomed Chromatogr (2012) 0.87

Inactivation of MARK4, an AMP-activated protein kinase (AMPK)-related kinase, leads to insulin hypersensitivity and resistance to diet-induced obesity. J Biol Chem (2012) 0.87

Comparison of microbial diversity determined with the same variable tag sequence extracted from two different PCR amplicons. BMC Microbiol (2013) 0.87

Interleukin-12 treatment down-regulates STAT4 and induces apoptosis with increasing ROS production in human natural killer cells. J Leukoc Biol (2011) 0.87

Competitive protection of aptamer-functionalized gold nanoparticles by controlling the DNA assembly. Anal Chem (2011) 0.87

Direct contact with mineral trioxide aggregate activates and differentiates human dental pulp cells. J Endod (2011) 0.86

Prostaglandin E2 upregulates survivin expression via the EP1 receptor in hepatocellular carcinoma cells. Life Sci (2009) 0.86

Role of epithelial to mesenchymal transition proteins in gynecological cancers: pathological and therapeutic perspectives. Tumour Biol (2014) 0.86

Prostaglandin E2 receptor EP1 phosphorylate CREB and mediates MMP2 expression in human cholangiocarcinoma cells. Mol Cell Biochem (2013) 0.86

SAD-A kinase controls islet β-cell size and function as a mediator of mTORC1 signaling. Proc Natl Acad Sci U S A (2013) 0.85

Binding-induced DNA assembly and its application to yoctomole detection of proteins. Anal Chem (2011) 0.85

Epigenetics and cervical cancer: from pathogenesis to therapy. Tumour Biol (2014) 0.85

Production of Shiga-like toxins in viable but nonculturable Escherichia coli O157:H7. Water Res (2009) 0.84

Single dose of morphine produced a prolonged effect on dopamine neuron activities. Mol Pain (2008) 0.84

Prostaglandin E2 promotes liver cancer cell growth by the upregulation of FUSE-binding protein 1 expression. Int J Oncol (2013) 0.84

Cytotoxicity assessment based on the AUC50 using multi-concentration time-dependent cellular response curves. Anal Chim Acta (2013) 0.84

Amorphous calcium phosphate and its application in dentistry. Chem Cent J (2011) 0.84

Electronic deoxyribonucleic acid (DNA) microarray detection of viable pathogenic Escherichia coli, Vibrio cholerae, and Salmonella typhi. Anal Chim Acta (2006) 0.84

L-stepholidine reduced L-DOPA-induced dyskinesia in 6-OHDA-lesioned rat model of Parkinson's disease. Neurobiol Aging (2008) 0.84

A toxic disinfection by-product, 2,6-dichloro-1,4-benzoquinone, identified in drinking water. Angew Chem Int Ed Engl (2010) 0.83

Interaction of oxaliplatin, cisplatin, and carboplatin with hemoglobin and the resulting release of a heme group. Chem Res Toxicol (2004) 0.83

Development of a microelectronic chip array for high-throughput genotyping of Helicobacter species and screening for antimicrobial resistance. J Biomol Screen (2005) 0.83

Laser capture microdissection enables cellular and molecular studies of tooth root development. Int J Oral Sci (2012) 0.83

A molecular translator that acts by binding-induced DNA strand displacement for a homogeneous protein assay. Angew Chem Int Ed Engl (2012) 0.83

Hydrophilic interaction liquid chromatography-tandem mass spectrometry determination of estrogen conjugates in human urine. Anal Chem (2008) 0.82

Tunable aptamer capillary electrophoresis and its application to protein analysis. J Am Chem Soc (2007) 0.82

FGF21 increases cholesterol efflux by upregulating ABCA1 through the ERK1/2-PPARγ-LXRα pathway in THP1 macrophage-derived foam cells. DNA Cell Biol (2014) 0.82

Effects of Mycobacterium tuberculosis ESAT-6/CFP-10 fusion protein on the autophagy function of mouse macrophages. DNA Cell Biol (2011) 0.82

Titanates deliver metal ions to human monocytes. J Mater Sci Mater Med (2009) 0.82

Prostaglandin E₂ receptor EP1-mediated phosphorylation of focal adhesion kinase enhances cell adhesion and migration in hepatocellular carcinoma cells. Int J Oncol (2013) 0.82

Identification of differentially expressed genes related to radioresistance of human esophageal cancer cells. Chin J Cancer (2010) 0.82

Qualitative and quantitative analysis of Fructus Corni using ultrasound assisted microwave extraction and high performance liquid chromatography coupled with diode array UV detection and time-of-flight mass spectrometry. J Pharm Biomed Anal (2011) 0.82

Detection of Escherichia coli O157:H7 using gold nanoparticle labeling and inductively coupled plasma mass spectrometry. Anal Chem (2010) 0.82

Aptamer-linked assay for thrombin using gold nanoparticle amplification and inductively coupled plasma-mass spectrometry detection. Anal Chem (2009) 0.82

Reconstitution of the enzyme AroA and its glyphosate tolerance by fragment complementation. FEBS Lett (2006) 0.82

Protective and therapeutic efficacy of Mycobacterium smegmatis expressing HBHA-hIL12 fusion protein against Mycobacterium tuberculosis in mice. PLoS One (2012) 0.81

Liquid chromatography tandem mass spectrometry in study of the pharmacokinetics of six steroidal saponins in rats. Steroids (2013) 0.81

"One-pot" methylation in glycomics application: esterification of sialic acids and permanent charge construction. Anal Chem (2007) 0.81

Mass spectrometric studies of cisplatin-induced changes of hemoglobin. Rapid Commun Mass Spectrom (2003) 0.81

Tracing intracellular localization and chemical forms of copper in Elsholtzia splendens with cluster analysis. Biol Trace Elem Res (2014) 0.81

Identification of multiple components in Guanxinning injection using hydrophilic interaction liquid chromatography/time-of-flight mass spectrometry and reversed-phase liquid chromatography/time-of-flight mass spectrometry. Rapid Commun Mass Spectrom (2011) 0.81

Yoctomole detection of proteins using solid phase binding-induced DNA assembly. Methods (2013) 0.81

Study of interactions between arsenicals and thioredoxins (human and E. coli) using mass spectrometry. Rapid Commun Mass Spectrom (2007) 0.81

Evaluation of the antipsychotic effect of bi-acetylated l-stepholidine (l-SPD-A), a novel dopamine and serotonin receptor dual ligand. Schizophr Res (2009) 0.81