Published in Nat Rev Immunol on August 01, 2004
Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science (2011) 15.45
SPICE: exploration and analysis of post-cytometric complex multivariate datasets. Cytometry A (2011) 5.78
Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses. J Exp Med (2007) 5.22
Automated high-dimensional flow cytometric data analysis. Proc Natl Acad Sci U S A (2009) 4.91
Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators. Nat Biotechnol (2012) 3.65
Multiple-cytokine-producing antiviral CD4 T cells are functionally superior to single-cytokine-producing cells. J Virol (2007) 3.23
Immunisation with BCG and recombinant MVA85A induces long-lasting, polyfunctional Mycobacterium tuberculosis-specific CD4+ memory T lymphocyte populations. Eur J Immunol (2007) 3.18
Bone marrow-derived endothelial progenitor cells are a major determinant of nascent tumor neovascularization. Genes Dev (2007) 2.60
Transient regulatory T-cells: a state attained by all activated human T-cells. Clin Immunol (2006) 2.56
Data reduction for spectral clustering to analyze high throughput flow cytometry data. BMC Bioinformatics (2010) 2.46
Elucidation of seventeen human peripheral blood B-cell subsets and quantification of the tetanus response using a density-based method for the automated identification of cell populations in multidimensional flow cytometry data. Cytometry B Clin Cytom (2010) 2.38
Single-Cell Genomics Unveils Critical Regulators of Th17 Cell Pathogenicity. Cell (2015) 2.30
Bacillus Calmette-Guérin vaccination of human newborns induces T cells with complex cytokine and phenotypic profiles. J Immunol (2008) 2.27
A chromatic explosion: the development and future of multiparameter flow cytometry. Immunology (2008) 2.17
Origins of regulated cell-to-cell variability. Nat Rev Mol Cell Biol (2011) 2.15
Systems biology in immunology: a computational modeling perspective. Annu Rev Immunol (2011) 2.15
Optimizing a multicolor immunophenotyping assay. Clin Lab Med (2007) 2.12
Flow cytometric identification and functional characterization of immature and mature circulating endothelial cells. Arterioscler Thromb Vasc Biol (2012) 2.07
Superior T memory stem cell persistence supports long-lived T cell memory. J Clin Invest (2013) 2.06
Polyfunctional responses by human T cells result from sequential release of cytokines. Proc Natl Acad Sci U S A (2011) 2.00
Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving. Lab Chip (2010) 1.97
Modern flow cytometry: a practical approach. Clin Lab Med (2007) 1.89
Polymer-based elemental tags for sensitive bioassays. Angew Chem Int Ed Engl (2007) 1.88
Single-cell technologies for monitoring immune systems. Nat Immunol (2014) 1.78
A model for harmonizing flow cytometry in clinical trials. Nat Immunol (2010) 1.70
Identification, isolation and in vitro expansion of human and nonhuman primate T stem cell memory cells. Nat Protoc (2012) 1.63
EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia (2012) 1.60
Histo-cytometry: a method for highly multiplex quantitative tissue imaging analysis applied to dendritic cell subset microanatomy in lymph nodes. Immunity (2012) 1.56
Detailed analysis of immunologic effects of the cytotoxic T lymphocyte-associated antigen 4-blocking monoclonal antibody tremelimumab in peripheral blood of patients with melanoma. J Transl Med (2008) 1.56
A survey of flow cytometry data analysis methods. Adv Bioinformatics (2009) 1.51
High-dose oral N-acetylcysteine, a glutathione prodrug, modulates inflammation in cystic fibrosis. Proc Natl Acad Sci U S A (2006) 1.47
Cholecalciferol supplementation alters calcitriol-responsive monocyte proteins and decreases inflammatory cytokines in ESRD. J Am Soc Nephrol (2009) 1.45
Institutional shared resources and translational cancer research. J Transl Med (2009) 1.41
Cytometry: today's technology and tomorrow's horizons. Methods (2012) 1.38
Identification of myeloid cell subsets in murine lungs using flow cytometry. Am J Respir Cell Mol Biol (2013) 1.37
The VLDL receptor promotes lipotoxicity and increases mortality in mice following an acute myocardial infarction. J Clin Invest (2011) 1.36
RchyOptimyx: cellular hierarchy optimization for flow cytometry. Cytometry A (2012) 1.35
Effector Memory T cells Are Associated With Atherosclerosis in Humans and Animal Models. J Am Heart Assoc (2012) 1.29
Polychromatic flow cytometric high-throughput assay to analyze the innate immune response to Toll-like receptor stimulation. J Immunol Methods (2008) 1.28
Short- and long-term effects of anti-CD20 treatment on B cell ontogeny in bone marrow of patients with rheumatoid arthritis. Arthritis Res Ther (2009) 1.26
Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers. Cytometry A (2008) 1.26
DNA gag/adenovirus type 5 (Ad5) gag and Ad5 gag/Ad5 gag vaccines induce distinct T-cell response profiles. J Virol (2008) 1.25
Single-cell analysis tools for drug discovery and development. Nat Rev Drug Discov (2015) 1.23
The development of quantum dot calibration beads and quantitative multicolor bioassays in flow cytometry and microscopy. Anal Biochem (2007) 1.22
Application of polychromatic flow cytometry to identify novel subsets of circulating cells with angiogenic potential. Cytometry A (2010) 1.22
Separating stem cells by flow cytometry: reducing variability for solid tissues. Cell Stem Cell (2009) 1.20
Immunogenic profiling in mice of a HIV/AIDS vaccine candidate (MVA-B) expressing four HIV-1 antigens and potentiation by specific gene deletions. PLoS One (2010) 1.20
Mice chronically fed high-fat diet have increased mortality and disturbed immune response in sepsis. PLoS One (2009) 1.18
Flow: Statistics, visualization and informatics for flow cytometry. Source Code Biol Med (2008) 1.17
At-risk and recent-onset type 1 diabetic subjects have increased apoptosis in the CD4+CD25+ T-cell fraction. PLoS One (2007) 1.15
A robust method for production of MHC tetramers with small molecule fluorophores. J Immunol Methods (2006) 1.15
Publishing flow cytometry data. Am J Physiol Lung Cell Mol Physiol (2009) 1.14
SWIFT-scalable clustering for automated identification of rare cell populations in large, high-dimensional flow cytometry datasets, part 1: algorithm design. Cytometry A (2014) 1.13
Automated analysis of multidimensional flow cytometry data improves diagnostic accuracy between mantle cell lymphoma and small lymphocytic lymphoma. Am J Clin Pathol (2012) 1.12
A candidate HIV/AIDS vaccine (MVA-B) lacking vaccinia virus gene C6L enhances memory HIV-1-specific T-cell responses. PLoS One (2011) 1.10
Modulation of cell signaling networks after CTLA4 blockade in patients with metastatic melanoma. PLoS One (2010) 1.09
NK cells and gamma interferon coordinate the formation and function of hepatic granulomas in mice infected with the Francisella tularensis live vaccine strain. Infect Immun (2008) 1.08
Single-cell profiling reveals the origin of phenotypic variability in adipogenesis. PLoS One (2009) 1.08
Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry. Cytometry A (2006) 1.07
Cell-based screening using high-throughput flow cytometry. Assay Drug Dev Technol (2010) 1.07
Single-cell protein analysis. Curr Opin Biotechnol (2011) 1.05
Formation and characterization of an ideal excitation beam geometry in an optofluidic device. Biomed Opt Express (2010) 1.05
High-throughput screening of large volumes of whole blood using structured illumination and fluorescent on-chip imaging. Lab Chip (2012) 1.05
Computational modeling of mammalian signaling networks. Wiley Interdiscip Rev Syst Biol Med (2010) 1.04
Flow cytometry for drug discovery, receptor pharmacology and high-throughput screening. Curr Opin Pharmacol (2007) 1.04
High throughput T epitope mapping and vaccine development. J Biomed Biotechnol (2010) 1.04
Deep Learning in Label-free Cell Classification. Sci Rep (2016) 1.03
High-throughput identification of potential minor histocompatibility antigens by MHC tetramer-based screening: feasibility and limitations. PLoS One (2011) 1.03
Computational analysis of high-throughput flow cytometry data. Expert Opin Drug Discov (2012) 1.03
Application of user-guided automated cytometric data analysis to large-scale immunoprofiling of invariant natural killer T cells. Proc Natl Acad Sci U S A (2013) 1.03
Phenotype and functional characterization of long-term gp100-specific memory CD8+ T cells in disease-free melanoma patients before and after boosting immunization. Clin Cancer Res (2008) 1.02
Single-cell technologies are revolutionizing the approach to rare cells. Immunol Cell Biol (2015) 1.01
Measuring enzyme activity in single cells. Trends Biotechnol (2011) 1.01
Human b1 cell frequency: isolation and analysis of human b1 cells. Front Immunol (2012) 1.00
Using the transcription factor inhibitor of DNA binding 1 to selectively target endothelial progenitor cells offers novel strategies to inhibit tumor angiogenesis and growth. Cancer Res (2010) 1.00
CD8(+) T-cell effector function and transcriptional regulation during HIV pathogenesis. Immunol Rev (2013) 1.00
Microfluidic CARS cytometry. Opt Express (2008) 1.00
Purification of specific cell population by fluorescence activated cell sorting (FACS). J Vis Exp (2010) 0.99
Comprehensive characterization of mesenchymal stem cells from human placenta and fetal membrane and their response to osteoactivin stimulation. Stem Cells Int (2012) 0.99
Single cell analysis using surface enhanced Raman scattering (SERS) tags. Methods (2012) 0.99
MHC-based detection of antigen-specific CD8+ T cell responses. Cancer Immunol Immunother (2010) 0.98
Modeling flow cytometry data for cancer vaccine immune monitoring. Cancer Immunol Immunother (2010) 0.98
SWIFT-scalable clustering for automated identification of rare cell populations in large, high-dimensional flow cytometry datasets, part 2: biological evaluation. Cytometry A (2014) 0.98
Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry. Nat Protoc (2010) 0.97
Measurement and modeling of signaling at the single-cell level. Biochemistry (2012) 0.96
Dynamic epitope expression from static cytometry data: principles and reproducibility. PLoS One (2012) 0.96
Cellular Composition of Cerebrospinal Fluid in HIV-1 Infected and Uninfected Subjects. PLoS One (2013) 0.96
Learning signaling network structures with sparsely distributed data. J Comput Biol (2009) 0.95
Amine-reactive dyes for dead cell discrimination in fixed samples. Curr Protoc Cytom (2010) 0.95
Single-cell mass cytometry reveals intracellular survival/proliferative signaling in FLT3-ITD-mutated AML stem/progenitor cells. Cytometry A (2015) 0.95
Regioselective 15-bromination and functionalization of a stable synthetic bacteriochlorin. J Org Chem (2007) 0.94
Bone marrow-derived Gr1+ cells can generate a metastasis-resistant microenvironment via induced secretion of thrombospondin-1. Cancer Discov (2013) 0.94
Association of two clones allows for optimal detection of human FOXP3. Cytometry A (2010) 0.94
Cytokine production and dysregulation in HIV pathogenesis: lessons for development of therapeutics and vaccines. Cytokine Growth Factor Rev (2012) 0.93
High-dimensional single-cell cancer biology. Curr Top Microbiol Immunol (2014) 0.92
Biomarkers in T cell therapy clinical trials. J Transl Med (2011) 0.92
Optimization of a highly standardized carboxyfluorescein succinimidyl ester flow cytometry panel and gating strategy design using discriminative information measure evaluation. Cytometry A (2010) 0.92
The intersection of flow cytometry with microfluidics and microfabrication. Lab Chip (2014) 0.91
HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells. Blood (2006) 17.38
Rational design of envelope identifies broadly neutralizing human monoclonal antibodies to HIV-1. Science (2010) 14.91
Massive infection and loss of memory CD4+ T cells in multiple tissues during acute SIV infection. Nature (2005) 13.15
Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation. J Immunol Methods (2003) 11.81
Multifunctional TH1 cells define a correlate of vaccine-mediated protection against Leishmania major. Nat Med (2007) 11.80
T-cell quality in memory and protection: implications for vaccine design. Nat Rev Immunol (2008) 11.65
PD-1 is a regulator of virus-specific CD8+ T cell survival in HIV infection. J Exp Med (2006) 8.18
Focused evolution of HIV-1 neutralizing antibodies revealed by structures and deep sequencing. Science (2011) 7.92
A human memory T cell subset with stem cell-like properties. Nat Med (2011) 7.47
Expression of CD57 defines replicative senescence and antigen-induced apoptotic death of CD8+ T cells. Blood (2002) 6.73
Accelerated vaccination for Ebola virus haemorrhagic fever in non-human primates. Nature (2003) 6.35
Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 candidate vaccine delivered by a replication-defective recombinant adenovirus vector. J Infect Dis (2006) 5.53
Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses. J Exp Med (2007) 5.22
Phenotype and function of human T lymphocyte subsets: consensus and issues. Cytometry A (2008) 4.71
Protection against malaria by intravenous immunization with a nonreplicating sporozoite vaccine. Science (2013) 4.60
Standardization of cytokine flow cytometry assays. BMC Immunol (2005) 4.55
Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry. Nat Med (2006) 4.41
T-cell subsets that harbor human immunodeficiency virus (HIV) in vivo: implications for HIV pathogenesis. J Virol (2004) 4.02
Emerging concepts in the immunopathogenesis of AIDS. Annu Rev Med (2009) 3.94
Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistent DNA viruses. J Exp Med (2005) 3.63
Ex vivo identification, isolation and analysis of tumor-cytolytic T cells. Nat Med (2003) 3.60
A new "Logicle" display method avoids deceptive effects of logarithmic scaling for low signals and compensated data. Cytometry A (2006) 3.49
Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 DNA candidate vaccine. J Infect Dis (2006) 3.40
Quality assurance for polychromatic flow cytometry. Nat Protoc (2006) 3.34
Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry. J Immunol Methods (2006) 3.32
Vaccination in humans generates broad T cell cytokine responses. J Immunol (2004) 3.27
Acquisition of direct antiviral effector functions by CMV-specific CD4+ T lymphocytes with cellular maturation. J Exp Med (2006) 3.26
Immunisation with BCG and recombinant MVA85A induces long-lasting, polyfunctional Mycobacterium tuberculosis-specific CD4+ memory T lymphocyte populations. Eur J Immunol (2007) 3.18
A deep profiler's guide to cytometry. Trends Immunol (2012) 3.14
A West Nile virus DNA vaccine induces neutralizing antibody in healthy adults during a phase 1 clinical trial. J Infect Dis (2007) 3.02
Intracellular cytokine optimization and standard operating procedure. Nat Protoc (2006) 2.96
SIV-specific CD8+ T cells express high levels of PD1 and cytokines but have impaired proliferative capacity in acute and chronic SIVmac251 infection. Blood (2007) 2.95
A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles. Nat Med (2005) 2.83
The history and future of the fluorescence activated cell sorter and flow cytometry: a view from Stanford. Clin Chem (2002) 2.71
Toll-like receptor agonists influence the magnitude and quality of memory T cell responses after prime-boost immunization in nonhuman primates. J Exp Med (2006) 2.70
Immunocompetent T-cells with a memory-like phenotype are the dominant cell type following antibody-mediated T-cell depletion. Am J Transplant (2005) 2.70
Phase I clinical evaluation of a six-plasmid multiclade HIV-1 DNA candidate vaccine. Vaccine (2007) 2.68
Role of antigen receptor affinity in T cell-independent antibody responses in vivo. Nat Immunol (2002) 2.64
Immune protection of nonhuman primates against Ebola virus with single low-dose adenovirus vectors encoding modified GPs. PLoS Med (2006) 2.64
Role of BCR affinity in T cell dependent antibody responses in vivo. Nat Immunol (2002) 2.63
HIV Gag protein conjugated to a Toll-like receptor 7/8 agonist improves the magnitude and quality of Th1 and CD8+ T cell responses in nonhuman primates. Proc Natl Acad Sci U S A (2005) 2.61
Vaccination preserves CD4 memory T cells during acute simian immunodeficiency virus challenge. J Exp Med (2006) 2.60
Polyfunctional T cell responses are a hallmark of HIV-2 infection. Eur J Immunol (2008) 2.59
CD4 T follicular helper cell dynamics during SIV infection. J Clin Invest (2012) 2.55
The cytolytic enzymes granyzme A, granzyme B, and perforin: expression patterns, cell distribution, and their relationship to cell maturity and bright CD57 expression. J Leukoc Biol (2008) 2.44
Human immunodeficiency virus type 1 neutralization measured by flow cytometric quantitation of single-round infection of primary human T cells. J Virol (2002) 2.44
A DNA vaccine for Ebola virus is safe and immunogenic in a phase I clinical trial. Clin Vaccine Immunol (2006) 2.39
Toll-like receptor ligands modulate dendritic cells to augment cytomegalovirus- and HIV-1-specific T cell responses. J Immunol (2003) 2.34
Priming immunization with DNA augments immunogenicity of recombinant adenoviral vectors for both HIV-1 specific antibody and T-cell responses. PLoS One (2010) 2.24
Demonstration of cross-protective vaccine immunity against an emerging pathogenic Ebolavirus Species. PLoS Pathog (2010) 2.18
Data analysis in flow cytometry: the future just started. Cytometry A (2010) 2.18
A chromatic explosion: the development and future of multiparameter flow cytometry. Immunology (2008) 2.17
High-throughput quantitative analysis of HIV-1 and SIV-specific ADCC-mediating antibody responses. Cytometry A (2011) 2.14
Optimizing a multicolor immunophenotyping assay. Clin Lab Med (2007) 2.12
Characterization of functional and phenotypic changes in anti-Gag vaccine-induced T cell responses and their role in protection after HIV-1 infection. Proc Natl Acad Sci U S A (2005) 2.10
Preferential infection and depletion of Mycobacterium tuberculosis-specific CD4 T cells after HIV-1 infection. J Exp Med (2010) 2.07
Superior T memory stem cell persistence supports long-lived T cell memory. J Clin Invest (2013) 2.06
Selective expansion of polyfunctional pathogen-specific CD4(+) T cells in HIV-1-infected patients with immune reconstitution inflammatory syndrome. Blood (2012) 2.03
Public clonotype usage identifies protective Gag-specific CD8+ T cell responses in SIV infection. J Exp Med (2009) 2.03
The functional profile of primary human antiviral CD8+ T cell effector activity is dictated by cognate peptide concentration. J Immunol (2004) 2.01
Beyond six colors: a new era in flow cytometry. Nat Med (2003) 1.94
Data exploration, quality control and testing in single-cell qPCR-based gene expression experiments. Bioinformatics (2012) 1.93
CD8+ cellular immunity mediates rAd5 vaccine protection against Ebola virus infection of nonhuman primates. Nat Med (2011) 1.83
Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination. J Virol (2010) 1.81
Gating-ML: XML-based gating descriptions in flow cytometry. Cytometry A (2008) 1.78
Resting naive CD4+ T cells are massively infected and eliminated by X4-tropic simian-human immunodeficiency viruses in macaques. Proc Natl Acad Sci U S A (2005) 1.75
The size of the viral inoculum contributes to the outcome of hepatitis B virus infection. J Virol (2009) 1.71
Safety, immunogenicity and efficacy of modified vaccinia Ankara (MVA) against Dryvax challenge in vaccinia-naïve and vaccinia-immune individuals. Vaccine (2006) 1.71
A model for harmonizing flow cytometry in clinical trials. Nat Immunol (2010) 1.70
Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome. Blood (2010) 1.66
The who's who of T-cell differentiation: human memory T-cell subsets. Eur J Immunol (2013) 1.63
Identification, isolation and in vitro expansion of human and nonhuman primate T stem cell memory cells. Nat Protoc (2012) 1.63
Single-cell gene-expression profiling reveals qualitatively distinct CD8 T cells elicited by different gene-based vaccines. Proc Natl Acad Sci U S A (2011) 1.56
Immunologic and virologic events in early HIV infection predict subsequent rate of progression. J Infect Dis (2010) 1.53
Differential association of programmed death-1 and CD57 with ex vivo survival of CD8+ T cells in HIV infection. J Immunol (2009) 1.52
Surface expression patterns of negative regulatory molecules identify determinants of virus-specific CD8+ T-cell exhaustion in HIV infection. Blood (2011) 1.52
Association of HIV-specific and total CD8+ T memory phenotypes in subtype C HIV-1 infection with viral set point. J Immunol (2009) 1.49
The flow cytometry shared resource laboratory: best practices to assure a high-quality, cost-effective partnership with biomedical research laboratories. Cytometry A (2009) 1.48
Early immunologic correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assays. Bioinformatics (2012) 1.46
T-cell dynamics during acute SIV infection. AIDS (2004) 1.46
Relationship between functional profile of HIV-1 specific CD8 T cells and epitope variability with the selection of escape mutants in acute HIV-1 infection. PLoS Pathog (2011) 1.42
Transient and persistent effects of IL-15 on lymphocyte homeostasis in nonhuman primates. Blood (2010) 1.41
Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expression. Nat Protoc (2006) 1.40
Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part I: Panel design by an empiric approach. Cytometry A (2008) 1.40
A SARS DNA vaccine induces neutralizing antibody and cellular immune responses in healthy adults in a Phase I clinical trial. Vaccine (2008) 1.39
Good cell, bad cell: flow cytometry reveals T-cell subsets important in HIV disease. Cytometry A (2010) 1.39
Safety (toxicity), pharmacokinetics, immunogenicity, and impact on elements of the normal immune system of recombinant human IL-15 in rhesus macaques. Blood (2011) 1.39
Cytometry: today's technology and tomorrow's horizons. Methods (2012) 1.38
Ontogeny of gamma delta T cells in humans. J Immunol (2004) 1.37
Brilliant violet fluorophores: a new class of ultrabright fluorescent compounds for immunofluorescence experiments. Cytometry A (2012) 1.37