Published in Biochem Biophys Res Commun on September 24, 2004
Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. Microb Cell Fact (2008) 1.89
Comparison of SUMO fusion technology with traditional gene fusion systems: enhanced expression and solubility with SUMO. Protein Sci (2005) 1.73
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors. Microb Cell Fact (2005) 1.59
A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. BMC Biotechnol (2006) 1.48
Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii. Plant Biotechnol J (2010) 1.39
Native folding of aggregation-prone recombinant proteins in Escherichia coli by osmolytes, plasmid- or benzyl alcohol-overexpressed molecular chaperones. Cell Stress Chaperones (2005) 1.32
Engineering inclusion bodies for non denaturing extraction of functional proteins. Microb Cell Fact (2008) 1.16
The TadV protein of Actinobacillus actinomycetemcomitans is a novel aspartic acid prepilin peptidase required for maturation of the Flp1 pilin and TadE and TadF pseudopilins. J Bacteriol (2006) 1.12
Structure of Smad1 MH1/DNA complex reveals distinctive rearrangements of BMP and TGF-beta effectors. Nucleic Acids Res (2010) 1.06
Production of recombinant disulfide-rich venom peptides for structural and functional analysis via expression in the periplasm of E. coli. PLoS One (2013) 1.00
Phospholipase Cζ rescues failed oocyte activation in a prototype of male factor infertility. Fertil Steril (2012) 0.95
Structure and stereospecificity of the dehydratase domain from the terminal module of the rifamycin polyketide synthase. Biochemistry (2013) 0.93
Self-assembly of virus-like particles of porcine circovirus type 2 capsid protein expressed from Escherichia coli. Virol J (2010) 0.90
Interaction of asymmetric ABCC9-encoded nucleotide binding domains determines KATP channel SUR2A catalytic activity. J Proteome Res (2008) 0.89
Recombinant production of human interleukin 6 in Escherichia coli. PLoS One (2013) 0.86
Galectin-1 as a fusion partner for the production of soluble and folded human beta-1,4-galactosyltransferase-T7 in E. coli. Biochem Biophys Res Commun (2010) 0.85
Loop interactions and dynamics tune the enzymatic activity of the human histone deacetylase 8. J Am Chem Soc (2013) 0.85
Sequence differences in the IQ motifs of CaV1.1 and CaV1.2 strongly impact calmodulin binding and calcium-dependent inactivation. J Biol Chem (2008) 0.84
Tenascin C promiscuously binds growth factors via its fifth fibronectin type III-like domain. PLoS One (2013) 0.84
Post-production protein stability: trouble beyond the cell factory. Microb Cell Fact (2011) 0.84
Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattle. Vet Res (2013) 0.83
Construction of an Expression Vector Containing Mtb72F of Mycobacterium tuberculosis. Cell J (2012) 0.81
High yield production of a soluble human interleukin-3 variant from E. coli with wild-type bioactivity and improved radiolabeling properties. PLoS One (2013) 0.81
Crystal optimization and preliminary diffraction data analysis of the Smad1 MH1 domain bound to a palindromic SBE DNA element. Acta Crystallogr Sect F Struct Biol Cryst Commun (2009) 0.80
Cloning, soluble expression and purification of high yield recombinant hGMCSF in Escherichia coli. Int J Mol Sci (2011) 0.79
Tracing Metabolite Footsteps of Escherichia coli Along the Time Course of Recombinant Protein Expression by Two-Dimensional NMR Spectroscopy. Bull Korean Chem Soc (2012) 0.78
Prokaryotic ubiquitin-like ThiS fusion enhances the heterologous protein overexpression and aggregation in Escherichia coli. PLoS One (2013) 0.78
Ubiquitin-like prokaryotic MoaD as a fusion tag for expression of heterologous proteins in Escherichia coli. BMC Biotechnol (2014) 0.75
Toxin Fused with SUMO Tag: A New Expression Vector Strategy to Obtain Recombinant Venom Toxins with Easy Tag Removal inside the Bacteria. Toxins (Basel) (2017) 0.75
Relationship between recombinant protein expression and host metabolome as determined by two-dimensional NMR spectroscopy. PLoS One (2017) 0.75
A fusion protein strategy for soluble expression of Stevia glycosyltransferase UGT76G1 in Escherichia coli. 3 Biotech (2017) 0.75
Immunity-related genes and gene families in Anopheles gambiae. Science (2002) 5.47
Evolutionary dynamics of immune-related genes and pathways in disease-vector mosquitoes. Science (2007) 4.03
Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP. Mol Cell (2003) 2.00
Malectin: a novel carbohydrate-binding protein of the endoplasmic reticulum and a candidate player in the early steps of protein N-glycosylation. Mol Biol Cell (2008) 1.94
Determinants of conformational dimerization of Mad2 and its inhibition by p31comet. EMBO J (2006) 1.89
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors. Microb Cell Fact (2005) 1.59
In vivo identification of novel regulators and conserved pathways of phagocytosis in A. gambiae. Immunity (2005) 1.52
Salmonella engineered to express CD20-targeting antibodies and a drug-converting enzyme can eradicate human lymphomas. Blood (2013) 1.49
NMR structure of the first PHD finger of autoimmune regulator protein (AIRE1). Insights into autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) disease. J Biol Chem (2005) 1.47
Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates. EMBO J (2005) 1.39
Characterization of the aggregates formed during recombinant protein expression in bacteria. BMC Biochem (2005) 1.38
Solution structure and ligand recognition of the WW domain pair of the yeast splicing factor Prp40. J Mol Biol (2002) 1.36
Meeting Report from the Second "Minimum Information for Biological and Biomedical Investigations" (MIBBI) workshop. Stand Genomic Sci (2010) 1.35
Crystal structure of the catalytic domain of Haspin, an atypical kinase implicated in chromatin organization. Proc Natl Acad Sci U S A (2009) 1.31
Screening of fusion partners for high yield expression and purification of bioactive viscotoxins. Protein Expr Purif (2008) 1.31
Unsupervised classification of single-particle X-ray diffraction snapshots by spectral clustering. Opt Express (2011) 1.27
The binding of NCAM to FGFR1 induces a specific cellular response mediated by receptor trafficking. J Cell Biol (2009) 1.23
Efficient protein depletion by genetically controlled deprotection of a dormant N-degron. Mol Syst Biol (2009) 1.12
Mosaic genome architecture of the Anopheles gambiae species complex. PLoS One (2007) 1.12
Biochemical and NMR analyses of an SF3b155-p14-U2AF-RNA interaction network involved in branch point definition during pre-mRNA splicing. RNA (2006) 1.09
Femtosecond free-electron laser x-ray diffraction data sets for algorithm development. Opt Express (2012) 1.07
Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60. J Biol Chem (2008) 1.06
Bacterial alpha2-macroglobulins: colonization factors acquired by horizontal gene transfer from the metazoan genome? Genome Biol (2004) 1.05
Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library. Protein Eng Des Sel (2009) 1.04
Knock-in of oncogenic Kras does not transform mouse somatic cells but triggers a transcriptional response that classifies human cancers. Cancer Res (2007) 1.01
Structure and phosphatidylinositol-(3,4)-bisphosphate binding of the C-terminal PH domain of human pleckstrin. Structure (2005) 0.98
Impairment of cytoplasmic eIF6 activity restricts lymphomagenesis and tumor progression without affecting normal growth. Cancer Cell (2011) 0.98
Domain substructure of HPV E6 oncoprotein: biophysical characterization of the E6 C-terminal DNA-binding domain. Biochemistry (2003) 0.97
The osmolyte betaine promotes protein misfolding and disruption of protein aggregates. Proteins (2009) 0.92
Genetically encoded FRET probe for PKC activity based on pleckstrin. J Am Chem Soc (2004) 0.91
Structure and dynamics of the human pleckstrin DEP domain: distinct molecular features of a novel DEP domain subfamily. Proteins (2005) 0.88
Improved quantitative and qualitative production of single-domain intrabodies mediated by the co-expression of Erv1p sulfhydryl oxidase. Protein Expr Purif (2011) 0.88
Comparative analysis of protein aggregates by blue native electrophoresis and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis in a three-dimensional geometry gel. Proteomics (2005) 0.88
Thioredoxin as a fusion tag for carrier-driven crystallization. Protein Sci (2008) 0.87
Single domain antibodies with VH hallmarks are positively selected during panning of llama (Lama glama) naïve libraries. Dev Comp Immunol (2011) 0.86
Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains. BMC Biotechnol (2007) 0.84
Screening optimized protein purification protocols by coupling small-scale expression and mini-size exclusion chromatography. Protein Expr Purif (2010) 0.84
Induced folding of the U2AF35 RRM upon binding to U2AF65. FEBS Lett (2002) 0.84
Expression, purification and preliminary crystallographic characterization of a novel segment from the neurofibromatosis type 1 protein. Acta Crystallogr D Biol Crystallogr (2004) 0.84
Recent contributions in the field of the recombinant expression of disulfide bonded proteins in bacteria. Microb Cell Fact (2012) 0.83
Reagent validation: an underestimated issue in laboratory practice. J Mol Recognit (2011) 0.83
Induced fit of passenger proteins fused to Archaea maltose binding proteins. Biochem Biophys Res Commun (2006) 0.82
Recombinant protein quality evaluation: proposal for a minimal information standard. Stand Genomic Sci (2011) 0.82
Monoclonal antibodies isolated by large-scale screening are suitable for labeling adult zebrafish (Danio rerio) tissues and cell structures. J Immunol Methods (2009) 0.82
Calcium binding by the PKD1 domain regulates interdomain flexibility in Vibrio cholerae metalloprotease PrtV. FEBS Open Bio (2013) 0.82
Single- and dual-parameter FRET kinase probes based on pleckstrin. Nat Protoc (2006) 0.82
Antibody purification-independent microarrays (PIM) by direct bacteria spotting on TiO2-treated slides. Methods (2011) 0.81
A monoclonal antibody against mutated nucleophosmin 1 for the molecular diagnosis of acute myeloid leukemias. Blood (2010) 0.81
The evaluation of the factors that cause aggregation during recombinant expression in E. coli is simplified by the employment of an aggregation-sensitive reporter. Microb Cell Fact (2006) 0.81
Bacterial expression, purification and preliminary X-ray crystallographic characterization of the invertase inhibitor Nt-CIF from tobacco. Acta Crystallogr D Biol Crystallogr (2003) 0.81
Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies. Microb Cell Fact (2014) 0.80
Antibody-mediated purification of co-expressed antigen-antibody complexes. Protein Expr Purif (2010) 0.80
The solubility of recombinant proteins expressed in Escherichia coli is increased by otsA and otsB co-transformation. Biochem Biophys Res Commun (2007) 0.79
Fusion tags and chaperone co-expression modulate both the solubility and the inclusion body features of the recombinant CLIPB14 serine protease. J Biotechnol (2005) 0.79
Crystallization of the actin-binding domain of human alpha-actinin: analysis of microcrystals of SeMet-labelled protein. Acta Crystallogr D Biol Crystallogr (2003) 0.79
Comparison and critical analysis of robotized technology for monoclonal antibody high-throughput production. Biotechnol Prog (2011) 0.78
Automated protein analysis by online detection of laser-induced fluorescence in slab gels and 3-D geometry gels. Electrophoresis (2006) 0.78
Antigenic features of protein carriers commonly used in immunisation trials. Biotechnol Lett (2010) 0.78
Single-domain antibodies that compete with the natural ligand fibroblast growth factor block the internalization of the fibroblast growth factor receptor 1. Biochem Biophys Res Commun (2011) 0.78
Structure and dynamics of the human muscle LIM protein. FEBS Lett (2009) 0.77
The availability of a recombinant anti-SNAP antibody in VHH format amplifies the application flexibility of SNAP-tagged proteins. J Biomed Biotechnol (2010) 0.77
Defining the minimal interacting regions of the tight junction protein MAGI-1 and HPV16 E6 oncoprotein for solution structure studies. Protein Expr Purif (2008) 0.77
Conflict of interests: multiple signal peptides with diverging goals. J Cell Biochem (2013) 0.76
Crystallization and X-ray analysis of a bacterial non-haem iron-containing phenylalanine hydroxylase from the Gram-negative opportunistic pathogen Pseudomonas aeruginosa. Acta Crystallogr D Biol Crystallogr (2003) 0.76
Physical and chemical perturbations induce the formation of protein aggregates with different structural features. Protein Expr Purif (2007) 0.76
Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivo. BMC Biotechnol (2009) 0.75
Purification, crystallization and preliminary data analysis of FocB, a transcription factor regulating fimbrial adhesin expression in uropathogenic Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun (2010) 0.75
A (musical) note on protein purification. FASEB J (2010) 0.75
1H, 13C, and 15N assignment of the muscular LIM protein MLP/CRP3. Biomol NMR Assign (2007) 0.75
Preparation of a naïve library of camelid single domain antibodies. Methods Mol Biol (2012) 0.75