Published in Chem Commun (Camb) on September 27, 2004
Structural and enzymatic analysis of MshA from Corynebacterium glutamicum: substrate-assisted catalysis. J Biol Chem (2008) 1.59
Chemical approaches to perturb, profile, and perceive glycans. Acc Chem Res (2009) 1.33
The crystal structures of the open and catalytically competent closed conformation of Escherichia coli glycogen synthase. J Biol Chem (2009) 1.18
Structure of Streptomyces maltosyltransferase GlgE, a homologue of a genetically validated anti-tuberculosis target. J Biol Chem (2011) 1.16
Structural and functional analysis of a new subfamily of glycosyltransferases required for glycosylation of serine-rich streptococcal adhesins. J Biol Chem (2011) 0.98
Substrate-induced conformational changes in the essential peripheral membrane-associated mannosyltransferase PimA from mycobacteria: implications for catalysis. J Biol Chem (2009) 0.93
Substrate-induced conformational changes and dynamics of UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferase-2. J Mol Biol (2007) 0.88
Rice Os9BGlu31 is a transglucosidase with the capacity to equilibrate phenylpropanoid, flavonoid, and phytohormone glycoconjugates. J Biol Chem (2013) 0.87
Glycosyltransferase mechanisms: impact of a 5-fluoro substituent in acceptor and donor substrates on catalysis. Biochemistry (2007) 0.86
A conserved acidic motif is crucial for enzymatic activity of protein O-mannosyltransferases. J Biol Chem (2011) 0.86
Structure-function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferases. Biochem J (2006) 0.83
N-acetylglucosaminidases from CAZy family GH3 are really glycoside phosphorylases, thereby explaining their use of histidine as an acid/base catalyst in place of glutamic acid. J Biol Chem (2014) 0.82
Structural analysis of Thermus thermophilus HB27 mannosyl-3-phosphoglycerate synthase provides evidence for a second catalytic metal ion and new insight into the retaining mechanism of glycosyltransferases. J Biol Chem (2010) 0.81
Microbial alkyl- and aryl-sulfatases: mechanism, occurrence, screening and stereoselectivities. Appl Microbiol Biotechnol (2013) 0.80
Regioselective glucosidation of trans-resveratrol in Escherichia coli expressing glucosyltransferase from Phytolacca americana. Biotechnol Lett (2011) 0.75
Breakdown of oligosaccharides by the process of elimination. Curr Opin Chem Biol (2006) 2.38
Pharmacological enhancement of beta-hexosaminidase activity in fibroblasts from adult Tay-Sachs and Sandhoff Patients. J Biol Chem (2004) 1.88
High-throughput screening for human lysosomal beta-N-Acetyl hexosaminidase inhibitors acting as pharmacological chaperones. Chem Biol (2007) 1.85
High-throughput screening methodology for the directed evolution of glycosyltransferases. Nat Methods (2006) 1.81
Using substrate engineering to harness enzymatic promiscuity and expand biological catalysis. Nat Chem Biol (2006) 1.63
Mechanism-based covalent neuraminidase inhibitors with broad-spectrum influenza antiviral activity. Science (2013) 1.61
Crystallographic structure of human beta-hexosaminidase A: interpretation of Tay-Sachs mutations and loss of GM2 ganglioside hydrolysis. J Mol Biol (2006) 1.59
Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog. Nat Struct Mol Biol (2004) 1.50
Engineering of glycosidases and glycosyltransferases. Curr Opin Chem Biol (2006) 1.49
The donor subsite of trehalose-6-phosphate synthase: binary complexes with UDP-glucose and UDP-2-deoxy-2-fluoro-glucose at 2 A resolution. J Biol Chem (2003) 1.43
Structural insights into the catalytic mechanism of Trypanosoma cruzi trans-sialidase. Structure (2004) 1.42
The search for novel human pancreatic alpha-amylase inhibitors: high-throughput screening of terrestrial and marine natural product extracts. Chembiochem (2008) 1.27
Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. J Biol Chem (2002) 1.26
Emerging methods for the production of homogeneous human glycoproteins. Nat Chem Biol (2009) 1.26
Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates. J Biol Chem (2003) 1.21
Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosis. J Biol Chem (2004) 1.20
The structure of Clostridium perfringens NanI sialidase and its catalytic intermediates. J Biol Chem (2008) 1.19
Intermediate trapping on a mutant retaining alpha-galactosyltransferase identifies an unexpected aspartate residue. J Biol Chem (2004) 1.16
Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state. Angew Chem Int Ed Engl (2002) 1.14
Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate. J Biol Chem (2004) 1.13
Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis. Structure (2004) 1.11
Structural and kinetic analysis of two covalent sialosyl-enzyme intermediates on Trypanosoma rangeli sialidase. J Biol Chem (2005) 1.11
Detailed comparative analysis of the catalytic mechanisms of beta-N-acetylglucosaminidases from families 3 and 20 of glycoside hydrolases. Biochemistry (2005) 1.09
Covalent inhibitors of glycosidases and their applications in biochemistry and biology. Glycobiology (2008) 1.08
Trypanosoma cruzi trans-sialidase operates through a covalent sialyl-enzyme intermediate: tyrosine is the catalytic nucleophile. J Am Chem Soc (2003) 1.06
Ultrahigh-throughput FACS-based screening for directed enzyme evolution. Chembiochem (2009) 1.06
Structural insight into mammalian sialyltransferases. Nat Struct Mol Biol (2009) 1.05
Conformational changes induced by binding UDP-2F-galactose to alpha-1,3 galactosyltransferase- implications for catalysis. J Mol Biol (2007) 1.05
Designer enzymes for glycosphingolipid synthesis by directed evolution. Nat Chem Biol (2009) 1.05
Structure and mechanism of chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum. Biochemistry (2006) 1.05
Imaging of enzyme replacement therapy using PET. Proc Natl Acad Sci U S A (2010) 1.03
Mechanistic analyses of catalysis in human pancreatic alpha-amylase: detailed kinetic and structural studies of mutants of three conserved carboxylic acids. Biochemistry (2002) 1.02
Iminoalditol-amino acid hybrids: synthesis and evaluation as glycosidase inhibitors. Carbohydr Res (2007) 1.01
An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima. J Am Chem Soc (2004) 1.01
Kinetic and mechanistic analysis of Trypanosoma cruzi trans-sialidase reveals a classical ping-pong mechanism with acid/base catalysis. Biochemistry (2008) 1.01
Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase. J Mol Biol (2004) 0.99
Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi. FEBS J (2006) 0.99
Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds α-maltose 1-phosphate and forms a maltosyl-enzyme intermediate. Biochemistry (2014) 0.98
Teaching old enzymes new tricks: engineering and evolution of glycosidases and glycosyl transferases for improved glycoside synthesis. Biochem Cell Biol (2008) 0.98
Glycosidase profiling with immobilised glycosidase-inhibiting iminoalditols--a proof-of-concept study. Bioorg Med Chem Lett (2008) 0.98
Structural and mechanistic analyses of endo-glycoceramidase II, a membrane-associated family 5 glycosidase in the Apo and GM3 ganglioside-bound forms. J Biol Chem (2007) 0.97
A new generation of specific Trypanosoma cruzi trans-sialidase inhibitors. Angew Chem Int Ed Engl (2008) 0.96
Thioglycoligases: mutant glycosidases for thioglycoside synthesis. Angew Chem Int Ed Engl (2003) 0.96
Structural analysis of the alpha-2,3-sialyltransferase Cst-I from Campylobacter jejuni in apo and substrate-analogue bound forms. Biochemistry (2007) 0.96
The structural basis for T-antigen hydrolysis by Streptococcus pneumoniae: a target for structure-based vaccine design. J Biol Chem (2008) 0.95
Detailed dissection of a new mechanism for glycoside cleavage: alpha-1,4-glucan lyase. Biochemistry (2003) 0.95
Glycoside cleavage by a new mechanism in unsaturated glucuronyl hydrolases. J Am Chem Soc (2011) 0.95
Direct demonstration of the flexibility of the glycosylated proline-threonine linker in the Cellulomonas fimi Xylanase Cex through NMR spectroscopic analysis. J Biol Chem (2006) 0.95
Active-site peptide "fingerprinting" of glycosidases in complex mixtures by mass spectrometry. Discovery of a novel retaining beta-1,4-glycanase in Cellulomonas fimi. J Biol Chem (2005) 0.95
Subsite structure of the endo-type chitin deacetylase from a deuteromycete, Colletotrichum lindemuthianum: an investigation using steady-state kinetic analysis and MS. Biochem J (2003) 0.95
Circular permutation of Bacillus circulans xylanase: a kinetic and structural study. Biochemistry (2010) 0.95
A beta-1,4-galactosyltransferase from Helicobacter pylori is an efficient and versatile biocatalyst displaying a novel activity for thioglycoside synthesis. Chembiochem (2008) 0.94
A homology model for human alpha-l-iduronidase: insights into human disease. Mol Genet Metab (2005) 0.93
Paenibacillus sp. TS12 glucosylceramidase: kinetic studies of a novel sub-family of family 3 glycosidases and identification of the catalytic residues. Biochem J (2004) 0.93
X-ray crystal structures of rabbit N-acetylglucosaminyltransferase I (GnT I) in complex with donor substrate analogues. J Mol Biol (2006) 0.93
Alternative donor substrates for inverting and retaining glycosyltransferases. Chem Commun (Camb) (2006) 0.92
Acarbose rearrangement mechanism implied by the kinetic and structural analysis of human pancreatic alpha-amylase in complex with analogues and their elongated counterparts. Biochemistry (2005) 0.92
Order and disorder: differential structural impacts of myricetin and ethyl caffeate on human amylase, an antidiabetic target. J Med Chem (2012) 0.91
Fluorescent glycosidase inhibiting 1,5-dideoxy-1,5-iminoalditols. Bioorg Med Chem Lett (2006) 0.90
Mechanistic analysis of trehalose synthase from Mycobacterium smegmatis. J Biol Chem (2011) 0.90
NAD+ and metal-ion dependent hydrolysis by family 4 glycosidases: structural insight into specificity for phospho-beta-D-glucosides. J Mol Biol (2005) 0.90
Unambiguous determination of the ionization state of a glycoside hydrolase active site lysine by 1H-15N heteronuclear correlation spectroscopy. J Am Chem Soc (2006) 0.90
Mechanistic studies of a retaining alpha-galactosyltransferase from Neisseria meningitidis. Biochemistry (2002) 0.89
Mechanism of GlvA from Bacillus subtilis: a detailed kinetic analysis of a 6-phospho-alpha-glucosidase from glycoside hydrolase family 4. Biochemistry (2007) 0.89
The mechanism of cellulose hydrolysis by a two-step, retaining cellobiohydrolase elucidated by structural and transition path sampling studies. J Am Chem Soc (2013) 0.88
Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis. J Biol Chem (2003) 0.87
Fluorescently tagged iminoalditol glycosidase inhibitors as novel biological probes and diagnostics. Bioorg Med Chem (2005) 0.87
The structure of the Mycobacterium smegmatis trehalose synthase reveals an unusual active site configuration and acarbose-binding mode. Glycobiology (2013) 0.87
Fluorescence activated cell sorting as a general ultra-high-throughput screening method for directed evolution of glycosyltransferases. J Am Chem Soc (2010) 0.87
Structural and mechanistic studies of chloride induced activation of human pancreatic alpha-amylase. Protein Sci (2005) 0.87
Structure and mechanism of the lipooligosaccharide sialyltransferase from Neisseria meningitidis. J Biol Chem (2011) 0.86
New approaches to enzymatic glycoside synthesis through directed evolution. Carbohydr Res (2010) 0.86
NMR spectroscopic characterization of the sialyltransferase CstII from Campylobacter jejuni: histidine 188 is the general base. Biochemistry (2009) 0.86
2-Acetamino-1,2-dideoxynojirimycin-lysine hybrids as hexosaminidase inhibitors. Tetrahedron Asymmetry (2009) 0.86
Probing the role of the chloride ion in the mechanism of human pancreatic alpha-amylase. Biochemistry (2002) 0.86
Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis. Chem Commun (Camb) (2009) 0.85
1-Deoxygalactonojirimycin-lysine hybrids as potent D-galactosidase inhibitors. Bioorg Med Chem (2008) 0.85
Anatomy of glycosynthesis: structure and kinetics of the Humicola insolens Cel7B E197A and E197S glycosynthase mutants. Chem Biol (2003) 0.85
Towards universal red blood cells. Nat Biotechnol (2007) 0.85
Synthesis and testing of 2-deoxy-2,2-dihaloglycosides as mechanism-based inhibitors of alpha-glycosidases. J Org Chem (2008) 0.85
Escherichia coli glucuronylsynthase: an engineered enzyme for the synthesis of beta-glucuronides. Org Lett (2008) 0.84
Mechanism of Thermoanaerobacterium saccharolyticum beta-xylosidase: kinetic studies. Biochemistry (2002) 0.84
An atypical approach identifies TYR234 as the key base catalyst in chondroitin AC lyase. Chembiochem (2006) 0.84
Insights into mucopolysaccharidosis I from the structure and action of α-L-iduronidase. Nat Chem Biol (2013) 0.84
Recruitment of both uniform and differential binding energy in enzymatic catalysis: xylanases from families 10 and 11. Biochemistry (2007) 0.84
Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides. Chem Commun (Camb) (2003) 0.84
Evaluation of a new system for developing particulate enzymes based on the surface (S)-layer protein (RsaA) of Caulobacter crescentus: fusion with the beta-1,4-glycanase (Cex) from the cellulolytic bacterium Cellulomonas fimi yields a robust, catalytically active product. Appl Biochem Biotechnol (2005) 0.84
Probing electrostatic interactions along the reaction pathway of a glycoside hydrolase: histidine characterization by NMR spectroscopy. Biochemistry (2007) 0.84
Mechanism, mutagenesis, and chemical rescue of a beta-mannosidase from cellulomonas fimi. Biochemistry (2003) 0.83
The chemical synthesis of 2-deoxy-2-fluorodisaccharide probes of the hen egg white lysozyme mechanism. Carbohydr Res (2005) 0.83
Alternative catalytic anions differentially modulate human alpha-amylase activity and specificity. Biochemistry (2008) 0.83
Structural and biochemical characterization of glycoside hydrolase family 79 β-glucuronidase from Acidobacterium capsulatum. J Biol Chem (2012) 0.83
Trapping covalent intermediates on beta-glycosidases. Methods Enzymol (2002) 0.83
Structural, mechanistic, and computational analysis of the effects of anomeric fluorines on anomeric fluoride departure in 5-fluoroxylosyl fluorides. J Am Chem Soc (2011) 0.83
Solid-phase oligosaccharide and glycopeptide synthesis using glycosynthases. J Org Chem (2002) 0.83
Directed evolution of a glycosynthase from Agrobacterium sp. increases its catalytic activity dramatically and expands its substrate repertoire. J Biol Chem (2004) 0.83
Biomining active cellulases from a mining bioremediation system. J Biotechnol (2013) 0.82
Rapid assembly of a library of lipophilic iminosugars via the thiol-ene reaction yields promising pharmacological chaperones for the treatment of Gaucher disease. J Med Chem (2012) 0.82
Engineering of a thioglycoligase: randomized mutagenesis of the acid-base residue leads to the identification of improved catalysts. Protein Eng Des Sel (2005) 0.82
Structure-based mutagenic analysis of mechanism and substrate specificity in mammalian glycosyltransferases: porcine ST3Gal-I. Glycobiology (2013) 0.82