Building and breeding molecules to spy on cells and tumors.

PubWeight™: 1.64‹?› | Rank: Top 3%

🔗 View Article (PMID 15680976)

Published in FEBS Lett on February 07, 2005

Authors

Roger Y Tsien1

Author Affiliations

1: Department of Pharmacology, University of California, San Diego, Howard Hughes Medical Institute, 310 George Palade Laboratories 0647, La Jolla, CA 92093-0647, USA. rtsien@ucsd.edu

Articles citing this

Going deeper than microscopy: the optical imaging frontier in biology. Nat Methods (2010) 5.65

Remodeling and homeostasis of the extracellular matrix: implications for fibrotic diseases and cancer. Dis Model Mech (2011) 2.97

Volumetric tomography of fluorescent proteins through small animals in vivo. Proc Natl Acad Sci U S A (2005) 2.16

Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells. Chem Rev (2011) 1.88

In vivo imaging and differential localization of lipid-modified GFP-variant fusions in embryonic stem cells and mice. Genesis (2006) 1.85

Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters. Proc Natl Acad Sci U S A (2008) 1.80

Soft X-ray tomography and cryogenic light microscopy: the cool combination in cellular imaging. Trends Cell Biol (2009) 1.71

Genetically encoded fluorescent sensors of membrane potential. Brain Cell Biol (2008) 1.57

Frontiers in optical imaging of cerebral blood flow and metabolism. J Cereb Blood Flow Metab (2012) 1.36

An in vivo biosensor for neurotransmitter release and in situ receptor activity. Nat Neurosci (2009) 1.36

Quantitative analysis of yeast internal architecture using soft X-ray tomography. Yeast (2010) 1.30

Fast complementation of split fluorescent protein triggered by DNA hybridization. Proc Natl Acad Sci U S A (2006) 1.26

Optical imaging of matrix metalloproteinase-7 activity in vivo using a proteolytic nanobeacon. Mol Imaging (2009) 1.25

Molecular analysis of mitotic chromosome condensation using a quantitative time-resolved fluorescence microscopy assay. Proc Natl Acad Sci U S A (2006) 1.18

Imaging proteins inside cells with fluorescent tags. Trends Biotechnol (2011) 1.17

Fluorogenic dendrons with multiple donor chromophores as bright genetically targeted and activated probes. J Am Chem Soc (2010) 1.12

Living-cell microarrays. Annu Rev Biomed Eng (2009) 1.12

Visualizing cell architecture and molecular location using soft x-ray tomography and correlated cryo-light microscopy. Annu Rev Phys Chem (2012) 1.12

Shining new light on 3D cell motility and the metastatic process. Trends Cell Biol (2009) 1.09

Live-cell fluorescence microscopy with molecular biosensors: what are we really measuring? Biophys J (2012) 1.08

Visualizing HIV-1 assembly. J Mol Biol (2011) 1.07

Determining the neurotransmitter concentration profile at active synapses. Mol Neurobiol (2009) 0.99

Monitoring activity in neural circuits with genetically encoded indicators. Front Mol Neurosci (2014) 0.97

Molecular imaging with nucleic acid aptamers. Curr Med Chem (2011) 0.96

Imaging beyond the proteome. Chem Commun (Camb) (2012) 0.95

Structural basis of fluorescence fluctuation dynamics of green fluorescent proteins in acidic environments. J Phys Chem B (2006) 0.94

Nucleic Acid aptamers: new methods for selection, stabilization, and application in biomedical science. Biomol Ther (Seoul) (2013) 0.94

A DTC niche plexus surrounds the germline stem cell pool in Caenorhabditis elegans. PLoS One (2014) 0.92

In vivo investigation of breast cancer progression by use of an internal control. Neoplasia (2009) 0.90

Scavenger receptor class B, type I (SR-BI) homo-dimerizes via its C-terminal region: fluorescence resonance energy transfer analysis. Biochim Biophys Acta (2007) 0.89

Viral mutagenesis as a means for generating novel proteins. J Virol (2009) 0.88

FRET and mechanobiology. Integr Biol (Camb) (2009) 0.88

Design strategies of fluorescent biosensors based on biological macromolecular receptors. Sensors (Basel) (2010) 0.88

Photophysics and dihedral freedom of the chromophore in yellow, blue, and green fluorescent protein. J Phys Chem B (2009) 0.86

Fluorescence molecular tomography: principles and potential for pharmaceutical research. Pharmaceutics (2011) 0.86

Correlative cryogenic tomography of cells using light and soft x-rays. Ultramicroscopy (2013) 0.86

Correlative microscopy methods that maximize specimen fidelity and data completeness, and improve molecular localization capabilities. J Struct Biol (2013) 0.85

Resonance Energy Transfer Between Luminescent Quantum Dots and Diverse Fluorescent Protein Acceptors. J Phys Chem C Nanomater Interfaces (2009) 0.85

Live cell imaging and systems biology. Wiley Interdiscip Rev Syst Biol Med (2010) 0.85

From analog to digital: exploring cell dynamics with single quantum dots. Histochem Cell Biol (2005) 0.84

Genetically encoded fluorescent sensors for studying healthy and diseased nervous systems. Drug Discov Today Dis Models (2008) 0.83

Multiple-pulse pumping for enhanced fluorescence detection and molecular imaging in tissue. Methods (2013) 0.82

The Role of the Tight-Turn, Broken Hydrogen Bonding, Glu222 and Arg96 in the Post-translational Green Fluorescent Protein Chromophore Formation. Chem Phys (2008) 0.82

Molecular Imaging of Proteases in Cancer. Cancer Growth Metastasis (2009) 0.81

Ligand-induced dynamics of neurotrophin receptors investigated by single-molecule imaging approaches. Int J Mol Sci (2015) 0.80

Imaging and characterizing cells using tomography. Arch Biochem Biophys (2015) 0.80

Carbon nanotube biosensors. Front Chem (2015) 0.77

Epi-fluorescence microscopy. Methods Mol Biol (2013) 0.77

Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases. Proteomes (2015) 0.75

Hyperspectral Imaging Using Intracellular Spies: Quantitative Real-Time Measurement of Intracellular Parameters In Vivo during Interaction of the Pathogenic Fungus Aspergillus fumigatus with Human Monocytes. PLoS One (2016) 0.75

Microscopy and image analysis. Curr Protoc Hum Genet (2005) 0.75

Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell. Biol Cell (2016) 0.75

Biosensors and their applications - A review. J Oral Biol Craniofac Res (2016) 0.75

Intraoperative mapping of sentinel lymph node metastases using a clinically translated ultrasmall silica nanoparticle. Wiley Interdiscip Rev Nanomed Nanobiotechnol (2015) 0.75