Published in EMBO J on May 26, 2005
Fiber formation across the bacterial outer membrane by the chaperone/usher pathway. Cell (2008) 2.69
Evolution of the chaperone/usher assembly pathway: fimbrial classification goes Greek. Microbiol Mol Biol Rev (2007) 2.44
Structural biology of the chaperone-usher pathway of pilus biogenesis. Nat Rev Microbiol (2009) 2.19
Rationally designed small compounds inhibit pilus biogenesis in uropathogenic bacteria. Proc Natl Acad Sci U S A (2006) 1.87
Crystal structure of the FimD usher bound to its cognate FimC-FimH substrate. Nature (2011) 1.67
Automated amino acid side-chain NMR assignment of proteins using (13)C- and (15)N-resolved 3D [ (1)H, (1)H]-NOESY. J Biomol NMR (2008) 1.49
Architectures and biogenesis of non-flagellar protein appendages in Gram-negative bacteria. EMBO J (2008) 1.48
A tale of two pili: assembly and function of pili in bacteria. Trends Microbiol (2010) 1.47
Small but versatile: the extraordinary functional and structural diversity of the beta-grasp fold. Biol Direct (2007) 1.31
Design and synthesis of C-2 substituted thiazolo and dihydrothiazolo ring-fused 2-pyridones: pilicides with increased antivirulence activity. J Med Chem (2010) 1.22
Chaperone-usher pathways: diversity and pilus assembly mechanism. Philos Trans R Soc Lond B Biol Sci (2012) 1.18
Insights into pilus assembly and secretion from the structure and functional characterization of usher PapC. Proc Natl Acad Sci U S A (2009) 1.09
Mechanism of fibre assembly through the chaperone-usher pathway. EMBO Rep (2006) 1.06
Modulating effects of the plug, helix, and N- and C-terminal domains on channel properties of the PapC usher. J Biol Chem (2009) 1.04
Adaptive mutations in the signal peptide of the type 1 fimbrial adhesin of uropathogenic Escherichia coli. Proc Natl Acad Sci U S A (2008) 1.00
The differential affinity of the usher for chaperone-subunit complexes is required for assembly of complete pili. Mol Microbiol (2010) 0.95
Quality control of disulfide bond formation in pilus subunits by the chaperone FimC. Nat Chem Biol (2012) 0.94
Function of the usher N-terminus in catalysing pilus assembly. Mol Microbiol (2010) 0.91
Domain activities of PapC usher reveal the mechanism of action of an Escherichia coli molecular machine. Proc Natl Acad Sci U S A (2012) 0.90
Structural homology between the C-terminal domain of the PapC usher and its plug. J Bacteriol (2010) 0.88
Structure and function of enterotoxigenic Escherichia coli fimbriae from differing assembly pathways. Mol Microbiol (2014) 0.85
Dissection of pilus tip assembly by the FimD usher monomer. J Mol Biol (2013) 0.84
Use of a combined cryo-EM and X-ray crystallography approach to reveal molecular details of bacterial pilus assembly by the chaperone/usher pathway. Curr Opin Microbiol (2009) 0.83
The pilus usher controls protein interactions via domain masking and is functional as an oligomer. Nat Struct Mol Biol (2015) 0.81
The role of chaperone-subunit usher domain interactions in the mechanism of bacterial pilus biogenesis revealed by ESI-MS. Mol Cell Proteomics (2012) 0.81
Ordered and ushered; the assembly and translocation of the adhesive type I and p pili. Biology (Basel) (2013) 0.80
Chemical biology applied to the study of bacterial pathogens. Infect Immun (2014) 0.79
Suppression of type 1 pilus assembly in uropathogenic Escherichia coli by chemical inhibition of subunit polymerization. J Antimicrob Chemother (2013) 0.78
Electrostatic networks control plug stabilization in the PapC usher. Mol Membr Biol (2016) 0.77
Microbiology: sensing stability. Nat Chem Biol (2012) 0.75
Nitazoxanide Inhibits Pilus Biogenesis by Interfering with Folding of the Usher Protein in the Outer Membrane. Antimicrob Agents Chemother (2016) 0.75
Whole-genome sequencing and comparative genomic analysis of Escherichia coli O91 strains isolated from symptomatic and asymptomatic human carriers. Gut Pathog (2016) 0.75
Structure, Function, and Assembly of Adhesive Organelles by Uropathogenic Bacteria. Microbiol Spectr (2015) 0.75
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res (1994) 392.47
The Protein Data Bank. Nucleic Acids Res (2000) 187.10
Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr (1998) 169.28
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A (2000) 96.90
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol (1995) 46.37
MOLMOL: a program for display and analysis of macromolecular structures. J Mol Graph (1996) 39.73
Touring protein fold space with Dali/FSSP. Nucleic Acids Res (1998) 18.00
Torsion angle dynamics for NMR structure calculation with the new program DYANA. J Mol Biol (1997) 15.40
Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. Proc Natl Acad Sci U S A (1997) 13.28
New method for generating deletions and gene replacements in Escherichia coli. J Bacteriol (1989) 12.66
The program XEASY for computer-supported NMR spectral analysis of biological macromolecules. J Biomol NMR (1995) 12.25
Protein NMR structure determination with automated NOE assignment using the new software CANDID and the torsion angle dynamics algorithm DYANA. J Mol Biol (2002) 11.20
ALSCRIPT: a tool to format multiple sequence alignments. Protein Eng (1993) 10.04
Induction and evasion of host defenses by type 1-piliated uropathogenic Escherichia coli. Science (1998) 8.16
Type 1 pilus-mediated bacterial invasion of bladder epithelial cells. EMBO J (2000) 5.03
X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli. Science (1999) 4.78
Protein NMR structure determination with automated NOE-identification in the NOESY spectra using the new software ATNOS. J Biomol NMR (2002) 4.55
Structural characterisation and functional significance of transient protein-protein interactions. J Mol Biol (2003) 3.72
Structural basis of chaperone function and pilus biogenesis. Science (1999) 3.69
FimH adhesin of type 1 pili is assembled into a fibrillar tip structure in the Enterobacteriaceae. Proc Natl Acad Sci U S A (1995) 3.51
Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation. Cell (2003) 3.15
Chaperone priming of pilus subunits facilitates a topological transition that drives fiber formation. Cell (2002) 2.54
A sequence-profile-based HMM for predicting and discriminating beta barrel membrane proteins. Bioinformatics (2002) 2.23
The new program OPAL for molecular dynamics simulations and energy refinements of biological macromolecules. J Biomol NMR (1996) 2.23
Uroplakin Ia is the urothelial receptor for uropathogenic Escherichia coli: evidence from in vitro FimH binding. J Cell Sci (2001) 2.13
Multiple pathways allow protein secretion across the bacterial outer membrane. Curr Opin Cell Biol (2000) 2.12
Structural polymorphism of bacterial adhesion pili. Nature (1995) 2.12
Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts. J Clin Microbiol (1980) 2.03
Fiber assembly by the chaperone-usher pathway. Biochim Biophys Acta (2004) 1.89
The fimD gene required for cell surface localization of Escherichia coli type 1 fimbriae. Mol Gen Genet (1990) 1.89
FimC is a periplasmic PapD-like chaperone that directs assembly of type 1 pili in bacteria. Proc Natl Acad Sci U S A (1993) 1.86
Exploring the 3D molecular architecture of Escherichia coli type 1 pili. J Mol Biol (2002) 1.81
Ramifications of kinetic partitioning on usher-mediated pilus biogenesis. EMBO J (1998) 1.76
Chaperone-assisted self-assembly of pili independent of cellular energy. J Biol Chem (1994) 1.74
Survival of FimH-expressing enterobacteria in macrophages relies on glycolipid traffic. Nature (1997) 1.72
Pilus chaperones represent a new type of protein-folding catalyst. Nature (2004) 1.46
Snapshots of usher-mediated protein secretion and ordered pilus assembly. Proc Natl Acad Sci U S A (2000) 1.40
The usher N terminus is the initial targeting site for chaperone-subunit complexes and participates in subsequent pilus biogenesis events. J Bacteriol (2004) 1.35
Pilus chaperone FimC-adhesin FimH interactions mapped by TROSY-NMR. Nat Struct Biol (1999) 1.23
The outer membrane usher forms a twin-pore secretion complex. J Mol Biol (2004) 1.23
Identification and characterization of the chaperone-subunit complex-binding domain from the type 1 pilus assembly platform FimD. J Mol Biol (2003) 1.16
Ambiguous distance data in the calculation of NMR structures. Fold Des (1997) 1.08
Chaperone-subunit-usher interactions required for donor strand exchange during bacterial pilus assembly. J Bacteriol (2003) 1.04
Chaperone-independent folding of type 1 pilus domains. J Mol Biol (2002) 1.01
Topology of the outer membrane usher PapC determined by site-directed fluorescence labeling. J Biol Chem (2004) 0.95
Probing conserved surfaces on PapD. Mol Microbiol (1999) 0.90
Epitope tagging analysis of the outer membrane folding of the molecular usher FaeD involved in K88 fimbriae biosynthesis in Escherichia coli. J Mol Microbiol Biotechnol (1999) 0.88
Protein NMR structure determination with automated NOE assignment using the new software CANDID and the torsion angle dynamics algorithm DYANA. J Mol Biol (2002) 11.20
TRIM5 is an innate immune sensor for the retrovirus capsid lattice. Nature (2011) 6.29
Protein NMR structure determination with automated NOE-identification in the NOESY spectra using the new software ATNOS. J Biomol NMR (2002) 4.55
An EB1-binding motif acts as a microtubule tip localization signal. Cell (2009) 4.08
High-affinity binders selected from designed ankyrin repeat protein libraries. Nat Biotechnol (2004) 3.34
Biased signaling pathways in β2-adrenergic receptor characterized by 19F-NMR. Science (2012) 3.07
Metabolic flux responses to pyruvate kinase knockout in Escherichia coli. J Bacteriol (2002) 3.06
Automated projection spectroscopy (APSY). Proc Natl Acad Sci U S A (2005) 2.91
Drug export pathway of multidrug exporter AcrB revealed by DARPin inhibitors. PLoS Biol (2007) 2.89
The long form of FLIP is an activator of caspase-8 at the Fas death-inducing signaling complex. J Biol Chem (2002) 2.75
Prion strain discrimination using luminescent conjugated polymers. Nat Methods (2007) 2.62
CASD-NMR: critical assessment of automated structure determination by NMR. Nat Methods (2009) 2.50
Effective rotational correlation times of proteins from NMR relaxation interference. J Magn Reson (2005) 2.37
Key interaction modes of dynamic +TIP networks. Mol Cell (2006) 2.18
NMR structure of the apoptosis- and inflammation-related NALP1 pyrin domain. Structure (2003) 2.15
NMR analysis of a 900K GroEL GroES complex. Nature (2002) 2.12
De novo generation of a transmissible spongiform encephalopathy by mouse transgenesis. Proc Natl Acad Sci U S A (2008) 2.10
Structure-function relationship of CAP-Gly domains. Nat Struct Mol Biol (2007) 2.02
Prion protein NMR structures of elk and of mouse/elk hybrids. Proc Natl Acad Sci U S A (2005) 2.01
Insights into the regulatory mechanism for caspase-8 activation. Mol Cell (2003) 2.01
Designed to be stable: crystal structure of a consensus ankyrin repeat protein. Proc Natl Acad Sci U S A (2003) 1.98
Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism. Structure (2007) 1.93
The JCSG high-throughput structural biology pipeline. Acta Crystallogr Sect F Struct Biol Cryst Commun (2010) 1.87
The structure of a cytolytic alpha-helical toxin pore reveals its assembly mechanism. Nature (2009) 1.84
Exploring the 3D molecular architecture of Escherichia coli type 1 pili. J Mol Biol (2002) 1.81
Allosteric inhibition of aminoglycoside phosphotransferase by a designed ankyrin repeat protein. Structure (2005) 1.75
Structure-based design of aliskiren, a novel orally effective renin inhibitor. Biochem Biophys Res Commun (2003) 1.74
TROSY-NMR reveals interaction between ERp57 and the tip of the calreticulin P-domain. Proc Natl Acad Sci U S A (2002) 1.70
Crystal structure of the multidrug exporter MexB from Pseudomonas aeruginosa. J Mol Biol (2009) 1.69
A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS. Nat Chem Biol (2012) 1.67
Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation. Nat Struct Mol Biol (2012) 1.66
Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop. Proc Natl Acad Sci U S A (2012) 1.66
Prion protein NMR structures of cats, dogs, pigs, and sheep. Proc Natl Acad Sci U S A (2005) 1.66
Membrane protein-lipid interactions in mixed micelles studied by NMR spectroscopy with the use of paramagnetic reagents. Chembiochem (2004) 1.62
Protein stabilization by salt bridges: concepts, experimental approaches and clarification of some misunderstandings. J Mol Recognit (2004) 1.61
Automated sequence-specific protein NMR assignment using the memetic algorithm MATCH. J Biomol NMR (2008) 1.59
Amyloid-beta aggregation. Neurodegener Dis (2007) 1.57
Recommendations of the wwPDB NMR Validation Task Force. Structure (2013) 1.57
ERp57 is a multifunctional thiol-disulfide oxidoreductase. J Biol Chem (2004) 1.55
Complementarity between a docking and a high-throughput screen in discovering new cruzain inhibitors. J Med Chem (2010) 1.54
Crystal structure and functional analysis of Escherichia coli glutamate decarboxylase. EMBO J (2003) 1.50
The GPCR Network: a large-scale collaboration to determine human GPCR structure and function. Nat Rev Drug Discov (2012) 1.50
NMR structure of the unliganded Bombyx mori pheromone-binding protein at physiological pH. FEBS Lett (2002) 1.49
Automated amino acid side-chain NMR assignment of proteins using (13)C- and (15)N-resolved 3D [ (1)H, (1)H]-NOESY. J Biomol NMR (2008) 1.49
Molecular basis of messenger RNA recognition by the specific bacterial repressing clamp RsmA/CsrA. Nat Struct Mol Biol (2007) 1.48
Direct NMR observation of a substrate protein bound to the chaperonin GroEL. Proc Natl Acad Sci U S A (2005) 1.47
Pilus chaperones represent a new type of protein-folding catalyst. Nature (2004) 1.46
Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters. Proc Natl Acad Sci U S A (2013) 1.46
NMR structure of the integral membrane protein OmpX. J Mol Biol (2004) 1.46
Reconstitution of pilus assembly reveals a bacterial outer membrane catalyst. Science (2008) 1.44
Molecular basis of coiled-coil formation. Proc Natl Acad Sci U S A (2007) 1.44
NMR structure of the bovine prion protein isolated from healthy calf brains. EMBO Rep (2004) 1.43
Cytotoxin ClyA from Escherichia coli assembles to a 13-meric pore independent of its redox-state. EMBO J (2006) 1.43
Amyloid formation by recombinant full-length prion proteins in phospholipid bicelle solutions. J Mol Biol (2006) 1.43
A molecular switch controls interspecies prion disease transmission in mice. J Clin Invest (2010) 1.41
NMR in drug discovery. Nat Rev Drug Discov (2002) 1.40
Solution structure and intermolecular interactions of the third metal-binding domain of ATP7A, the Menkes disease protein. J Biol Chem (2006) 1.40
Solution NMR structure determination of proteins revisited. J Biomol NMR (2008) 1.39
Protein structure determination from 13C spin-diffusion solid-state NMR spectroscopy. J Am Chem Soc (2008) 1.39
Structure of the PRYSPRY-domain: implications for autoinflammatory diseases. FEBS Lett (2005) 1.36
APSY-NMR with proteins: practical aspects and backbone assignment. J Biomol NMR (2008) 1.35
The recombinant amyloid-beta peptide Abeta1-42 aggregates faster and is more neurotoxic than synthetic Abeta1-42. J Mol Biol (2009) 1.35
Structural basis of ligand binding and release in insect pheromone-binding proteins: NMR structure of Antheraea polyphemus PBP1 at pH 4.5. J Mol Biol (2007) 1.35
Prion protein NMR structures of chickens, turtles, and frogs. Proc Natl Acad Sci U S A (2005) 1.34
Metabolic-flux profiling of the yeasts Saccharomyces cerevisiae and Pichia stipitis. Eukaryot Cell (2003) 1.33
Lipid-protein interactions in DHPC micelles containing the integral membrane protein OmpX investigated by NMR spectroscopy. Proc Natl Acad Sci U S A (2002) 1.32
Structural basis and kinetics of inter- and intramolecular disulfide exchange in the redox catalyst DsbD. EMBO J (2004) 1.32
Novel beta-barrel fold in the nuclear magnetic resonance structure of the replicase nonstructural protein 1 from the severe acute respiratory syndrome coronavirus. J Virol (2007) 1.30
Rapid proton-detected NMR assignment for proteins with fast magic angle spinning. J Am Chem Soc (2014) 1.29
The J-UNIO protocol for automated protein structure determination by NMR in solution. J Biomol NMR (2012) 1.28
Precision is essential for efficient catalysis in an evolved Kemp eliminase. Nature (2013) 1.28
Oligosaccharyltransferase: the central enzyme of N-linked protein glycosylation. J Inherit Metab Dis (2011) 1.27
NMR structure of a KlbA intein precursor from Methanococcus jannaschii. Protein Sci (2007) 1.27
Proteomics analysis unravels the functional repertoire of coronavirus nonstructural protein 3. J Virol (2008) 1.27
NMR screening and crystal quality of bacterially expressed prokaryotic and eukaryotic proteins in a structural genomics pipeline. Proc Natl Acad Sci U S A (2005) 1.23
Blind testing of routine, fully automated determination of protein structures from NMR data. Structure (2012) 1.23
NMR solution structure determination of membrane proteins reconstituted in detergent micelles. FEBS Lett (2003) 1.23
Monomeric and dimeric bZIP transcription factor GCN4 bind at the same rate to their target DNA site. Biochemistry (2004) 1.22
Fast resonance assignment and fold determination of human superoxide dismutase by high-resolution proton-detected solid-state MAS NMR spectroscopy. Angew Chem Int Ed Engl (2011) 1.21
Folding of a designed simple ankyrin repeat protein. Protein Sci (2004) 1.20
Microscale NMR screening of new detergents for membrane protein structural biology. J Am Chem Soc (2008) 1.18