Coupling of size-exclusion chromatography to a continuous assay for subtilisin using a fluorescence resonance energy transfer peptide substrate: testing of two standard inhibitors.

PubWeight™: 0.76‹?›

🔗 View Article (PMID 16038203)

Published in J Chromatogr A on July 22, 2005

Authors

Junko Hirata1, Lai Ping Chung, Freek Ariese, Hubertus Irth, Cees Gooijer

Author Affiliations

1: Department of Analytical Chemistry and Applied Spectroscopy, Division of Chemistry, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands. hirata@few.vu.nl

Articles by these authors

Analytical separation and detection methods for flavonoids. J Chromatogr A (2006) 1.36

Biosensor discovery of thyroxine transport disrupting chemicals. Toxicol Appl Pharmacol (2008) 1.04

Achievements in resonance Raman spectroscopy review of a technique with a distinct analytical chemistry potential. Anal Chim Acta (2007) 1.02

Solvent influence on excited-state intramolecular proton transfer in 3-hydroxychromone derivatives studied by cryogenic high-resolution fluorescence spectroscopy. Spectrochim Acta A Mol Biomol Spectrosc (2003) 1.01

Online fluorescence enhancement assay for the acetylcholine binding protein with parallel mass spectrometric identification. J Med Chem (2010) 0.96

Rapid dereplication of estrogenic compounds in pomegranate (Punica granatum) using on-line biochemical detection coupled to mass spectrometry. Phytochemistry (2004) 0.95

Analytical methods for determining metabolites of polycyclic aromatic hydrocarbon (PAH) pollutants in fish bile: A review. Environ Toxicol Pharmacol (2010) 0.93

Fluorescence rejection in resonance Raman spectroscopy using a picosecond-gated intensified charge-coupled device camera. Appl Spectrosc (2007) 0.92

Linearly moving low-volume spectroelectrochemical cell for microliter-scale surface-enhanced resonance Raman spectroscopy of heme proteins. Anal Chem (2004) 0.91

Surface plasmon resonance biosensor based fragment screening using acetylcholine binding protein identifies ligand efficiency hot spots (LE hot spots) by deconstruction of nicotinic acetylcholine receptor α7 ligands. J Med Chem (2010) 0.91

Determining acetylcholinesterase inhibitory activity in plant extracts using a fluorimetric flow assay. Phytochem Anal (2003) 0.90

On-line coupling of high-performance liquid chromatography to a continuous-flow enzyme assay based on electrospray ionization mass spectrometry. Anal Chem (2004) 0.89

Studying protein-protein affinity and immobilized ligand-protein affinity interactions using MS-based methods. Anal Bioanal Chem (2011) 0.88

Development of a selective ESI-MS derivatization reagent: synthesis and optimization for the analysis of aldehydes in biological mixtures. Anal Chem (2008) 0.88

Recent developments in protein-ligand affinity mass spectrometry. Anal Bioanal Chem (2010) 0.87

Determination and identification of estrogenic compounds generated with biosynthetic enzymes using hyphenated screening assays, high resolution mass spectrometry and off-line NMR. J Chromatogr B Analyt Technol Biomed Life Sci (2010) 0.87

Development of a countergradient parking system for gradient liquid chromatography with online biochemical detection of serine protease inhibitors. Anal Chem (2008) 0.87

Development of an online p38α mitogen-activated protein kinase binding assay and integration of LC-HR-MS. Anal Bioanal Chem (2010) 0.87

Advances in mass spectrometry-based post-column bioaffinity profiling of mixtures. Anal Bioanal Chem (2010) 0.86

A microfluidic-based enzymatic assay for bioactivity screening combined with capillary liquid chromatography and mass spectrometry. Lab Chip (2005) 0.86

Identification and quantification of the depurinating DNA adducts formed in mouse skin treated with dibenzo[a,l]pyrene (DB[a,l]P) or its metabolites and in rat mammary gland treated with DB[a,l]P. Chem Res Toxicol (2005) 0.86

Rapid on-line profiling of estrogen receptor binding metabolites of tamoxifen. J Med Chem (2006) 0.86

Combined theoretical and experimental deep-UV resonance raman studies of substituted pyrenes. J Phys Chem A (2005) 0.86

Online biochemical detection of glutathione-S-transferase P1-specific inhibitors in complex mixtures. J Biomol Screen (2007) 0.86

Liquid chromatography with atmospheric pressure chemical ionization and electrospray ionization mass spectrometry of flavonoids with triple-quadrupole and ion-trap instruments. J Chromatogr A (2003) 0.85

Metabolic profiling of endocrine-disrupting compounds by on-line cytochrome p450 bioreaction coupled to on-line receptor affinity screening. Chem Res Toxicol (2007) 0.84

Target immobilization as a strategy for NMR-based fragment screening: comparison of TINS, STD, and SPR for fragment hit identification. J Biomol Screen (2010) 0.84

Fatty acid profiling of raw human plasma and whole blood using direct thermal desorption combined with gas chromatography-mass spectrometry. J Chromatogr A (2007) 0.84

Rapid detection and identification of angiotensin-converting enzyme inhibitors by on-line liquid chromatography-biochemical detection, coupled to electrospray mass spectrometry. J Chromatogr A (2003) 0.83

Adolescent nicotine exposure transiently increases high-affinity nicotinic receptors and modulates inhibitory synaptic transmission in rat medial prefrontal cortex. FASEB J (2012) 0.83

Screening of protein-ligand interactions using dynamic protein-affinity chromatography solid-phase extraction-liquid chromatography-mass spectrometry. J Chromatogr A (2008) 0.83

A flow-through fluorescence polarization detection system for measuring GPCR-mediated modulation of cAMP production. J Biomol Screen (2007) 0.83

Monitoring and evaluation of the environmental dissipation of the marine antifoulant 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) in a Danish Harbor. Chemosphere (2004) 0.83

Structural elucidation of biologically active neomycin N-octyl derivatives in a regioisomeric mixture by means of liquid chromatography/ion trap time-of-flight mass spectrometry. Rapid Commun Mass Spectrom (2010) 0.83

Development of three parallel cytochrome P450 enzyme affinity detection systems coupled on-line to gradient high-performance liquid chromatography. Drug Metab Dispos (2007) 0.83

Development of an on-line weak-cation exchange liquid chromatography-tandem mass spectrometric method for screening aldehyde products in biological matrices. J Chromatogr B Analyt Technol Biomed Life Sci (2009) 0.83

Simple, rapid, and sensitive liquid chromatography-fluorescence method for the quantification of tranexamic acid in blood. J Chromatogr A (2007) 0.82

Biosensor recognition of thyroid-disrupting chemicals using transport proteins. Anal Chem (2006) 0.82

High throughput screening methodologies classified for major drug target classes according to target signaling pathways. Comb Chem High Throughput Screen (2010) 0.82

Development of a novel cytochrome p450 bioaffinity detection system coupled online to gradient reversed-phase high-performance liquid chromatography. J Biomol Screen (2005) 0.82

Picosecond Raman spectroscopy with a fast intensified CCD camera for depth analysis of diffusely scattering media. Analyst (2009) 0.82

Development of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assays. Anal Bioanal Chem (2010) 0.82

Time-resolved fluorescence of the bacteriophage T4 capsid protein gp23. J Photochem Photobiol B (2005) 0.82

On-line electrochemistry-bioaffinity screening with parallel HR-LC-MS for the generation and characterization of modified p38α kinase inhibitors. Anal Bioanal Chem (2012) 0.82

Targeted LC-MS derivatization for aldehydes and carboxylic acids with a new derivatization agent 4-APEBA. Anal Bioanal Chem (2010) 0.81

Structural rationalization of novel drug metabolizing mutants of cytochrome P450 BM3. Proteins (2008) 0.81

Towards combined electrochemistry and surface-enhanced resonance Raman of heme proteins: Improvement of diffusion electrochemistry of cytochrome c at silver electrodes chemically modified with 4-mercaptopyridine. Anal Chem (2006) 0.81

Production and on-line acetylcholinesterase bioactivity profiling of chemical and biological degradation products of tacrine. J Pharm Biomed Anal (2010) 0.81

Analysis of glutathione adducts of patulin by means of liquid chromatography (HPLC) with biochemical detection (BCD) and electrospray ionization tandem mass spectrometry (ESI-MS/MS). Anal Bioanal Chem (2009) 0.81

Microfractionation revisited: a 1536 well high resolution screening assay. Anal Chem (2009) 0.81

High-performance liquid chromatography-mass spectrometry-based acetylcholinesterase assay for the screening of inhibitors in natural extracts. J Chromatogr A (2006) 0.81

The application of HPLC-F and GC-MS to the analysis of selected hydroxy polycyclic hydrocarbons in two certified fish bile reference materials. J Environ Monit (2003) 0.80

Cytochrome P450 bio-affinity detection coupled to gradient HPLC: on-line screening of affinities to cytochrome P4501A2 and 2D6. J Chromatogr B Analyt Technol Biomed Life Sci (2007) 0.80

Hexamerization of the bacteriophage T4 capsid protein gp23 and its W13V mutant studied by time-resolved tryptophan fluorescence. J Phys Chem B (2006) 0.80

Probing the interaction of benzo[a]pyrene adducts and metabolites with monoclonal antibodies using fluorescence line-narrowing spectroscopy. Anal Chem (2004) 0.80

Active-site structure, binding and redox activity of the heme-thiolate enzyme CYP2D6 immobilized on coated Ag electrodes: a surface-enhanced resonance Raman scattering study. J Biol Inorg Chem (2007) 0.80

Determination of denaturated proteins and biotoxins by on-line size-exclusion chromatography-digestion-liquid chromatography-electrospray mass spectrometry. Anal Biochem (2005) 0.79

An on-line post-column detection system for the detection of reactive-oxygen-species-producing compounds and antioxidants in mixtures. Anal Bioanal Chem (2007) 0.79

High-resolution bioactivity profiling of mixtures toward the acetylcholine binding protein using a nanofractionation spotter technology. J Biomol Screen (2011) 0.79

High-temperature liquid chromatography coupled on-line to a continuous-flow biochemical screening assay with electrospray ionization mass spectrometric detection. Anal Chem (2005) 0.79

Liquid chromatography coupled to nuclear magnetic resonance spectroscopy for the identification of isoflavone glucoside malonates in T. pratense L. leaves. J Sep Sci (2004) 0.79

Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents. J Chromatogr B Analyt Technol Biomed Life Sci (2008) 0.79

Binding of bufuralol, dextromethorphan, and 3,4-methylenedioxymethylamphetamine to wild-type and F120A mutant cytochrome P450 2D6 studied by resonance Raman spectroscopy. Biochem Biophys Res Commun (2006) 0.79

Stereoselective binding of flurbiprofen enantiomers and their methyl esters to human serum albumin studied by time-resolved phosphorescence. Chirality (2012) 0.78

Tissue phantoms to compare spatial and temporal offset modes of deep Raman spectroscopy. Analyst (2015) 0.78

Development of surface plasmon resonance biosensor assays for primary and secondary screening of acetylcholine binding protein ligands. Anal Biochem (2010) 0.78

Flavonoids in Leguminosae: analysis of extracts of T. pratense L., T. dubium L., T. repens L., and L. corniculatus L. leaves using liquid chromatography with UV, mass spectrometric and fluorescence detection. Anal Bioanal Chem (2003) 0.78

Laser-induced quenched phosphorescence detection in capillary electrophoresis. Electrophoresis (2003) 0.78

Strong overtones and combination bands in ultraviolet resonance Raman spectroscopy. Anal Chem (2006) 0.78

Improved fatty acid detection in micro-algae and aquatic meiofauna species using a direct thermal desorption interface combined with comprehensive gas chromatography-time-of-flight mass spectrometry. J Chromatogr A (2008) 0.78

Sensitized enantioselective laser-induced phosphorescence detection in chiral capillary electrophoresis. Anal Chem (2010) 0.78

Development and validation of a microsomal online cytochrome P450 bioreactor coupled to solid-phase extraction and reversed-phase liquid chromatography. J Chromatogr A (2005) 0.78

Binding of 7-methoxy-4-(aminomethyl)-coumarin to wild-type and W128F mutant cytochrome P450 2D6 studied by time-resolved fluorescence spectroscopy. Biochem J (2006) 0.78

PAH biotransformation in terrestrial invertebrates--a new phase II metabolite in isopods and springtails. Comp Biochem Physiol C Toxicol Pharmacol (2004) 0.78

Trimethoprim: novel reactive intermediates and bioactivation pathways by cytochrome p450s. Chem Res Toxicol (2008) 0.78

Quenched phosphorescence as a detection method in capillary electrophoretic chiral separations. Monitoring the stereoselective biodegradation of camphorquinone by yeast. Anal Chem (2004) 0.78

Altered spin state equilibrium in the T309V mutant of cytochrome P450 2D6: a spectroscopic and computational study. J Biol Inorg Chem (2007) 0.77

Phosphorescence for sensitive enantioselective detection in chiral capillary electrophoresis. Anal Chem (2009) 0.77

Sensitized phosphorescence as detection method for the enantioseparation of bupropion by capillary electrophoresis. Electrophoresis (2010) 0.77

Changed isoflavone levels in red clover (Trifolium pratense L.) leaves with disturbed root nodulation in response to waterlogging. J Chem Ecol (2005) 0.77

Surface-enhanced resonance Raman scattering of cytochrome P450-2D6 on coated silver hydrosols. Langmuir (2007) 0.77

Automated detection of covalent adducts to human serum albumin by immunoaffinity chromatography, on-line solution phase digestion and liquid chromatography-mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci (2007) 0.77

Binding of naproxen enantiomers to human serum albumin studied by fluorescence and room-temperature phosphorescence. Spectrochim Acta A Mol Biomol Spectrosc (2012) 0.76