SiteFinding-PCR: a simple and efficient PCR method for chromosome walking.

A galactoglycerolipid lipase is required for triacylglycerol accumulation and survival following nitrogen deprivation in Chlamydomonas reinhardtii. Plant Cell (2012) 1.69

Self-formed adaptor PCR: a simple and efficient method for chromosome walking. Appl Environ Microbiol (2007) 1.54

Characterization of bacterial operons consisting of two tubulins and a kinesin-like gene by the novel Two-Step Gene Walking method. Nucleic Acids Res (2007) 1.45

Cre recombination-mediated cassette exchange for building versatile transgenic human embryonic stem cells lines. Stem Cells (2009) 1.26

In planta gene targeting. Proc Natl Acad Sci U S A (2012) 1.25

Members of the LATERAL ORGAN BOUNDARIES DOMAIN transcription factor family are involved in the regulation of secondary growth in Populus. Plant Cell (2010) 1.07

Purification and characterization of a novel laccase from Cerrena sp. HYB07 with dye decolorizing ability. PLoS One (2014) 1.06

TaCHP: a wheat zinc finger protein gene down-regulated by abscisic acid and salinity stress plays a positive role in stress tolerance. Plant Physiol (2010) 0.98

Cloning, expression, characterization, and biocatalytic investigation of the 4-hydroxyacetophenone monooxygenase from Pseudomonas putida JD1. Appl Environ Microbiol (2009) 0.92

The protein Compromised Hydrolysis of Triacylglycerols 7 (CHT7) acts as a repressor of cellular quiescence in Chlamydomonas. Proc Natl Acad Sci U S A (2014) 0.92

Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning. BMC Biotechnol (2011) 0.90

Assessing the risk that Phytophthora melonis can develop a point mutation (V1109L) in CesA3 conferring resistance to carboxylic acid amide fungicides. PLoS One (2012) 0.89

Inhibition of lipolysis in the novel transgenic quail model overexpressing G0/G1 switch gene 2 in the adipose tissue during feed restriction. PLoS One (2014) 0.89

Current and New Approaches in GMO Detection: Challenges and Solutions. Biomed Res Int (2015) 0.85

Cloning, expression, and characterization of a new beta-agarase from Vibrio sp. strain CN41. Appl Environ Microbiol (2011) 0.84

A conserved rubredoxin is necessary for photosystem II accumulation in diverse oxygenic photoautotrophs. J Biol Chem (2013) 0.83

Molecular evolution of pentatricopeptide repeat genes reveals truncation in species lacking an editing target and structural domains under distinct selective pressures. BMC Evol Biol (2012) 0.83

Cloning, expression, and characterization of an alkaline thermostable GH11 xylanase from Thermobifida halotolerans YIM 90462T. J Ind Microbiol Biotechnol (2012) 0.82

Restriction site-dependent PCR: an efficient technique for fast cloning of new genes of microorganisms. DNA Res (2007) 0.82

A new method to identify flanking sequence tags in chlamydomonas using 3'-RACE. Plant Methods (2012) 0.81

Cloning and characterization of the first polysaccharide lyase family 6 oligoalginate lyase from marine Shewanella sp. Kz7. J Biochem (2015) 0.80

A single glycine-alanine exchange directs ligand specificity of the elephant progestin receptor. PLoS One (2012) 0.80

Bioprospecting potential of halogenases from Arctic marine actinomycetes. BMC Microbiol (2016) 0.78

Cloning and knockout of phytoene desaturase gene in Sphingomonas elodea ATCC 31461 for economic recovery of gellan gum. J Ind Microbiol Biotechnol (2011) 0.77

A solute-binding protein for iron transport in Streptococcus iniae. BMC Microbiol (2010) 0.77

Engineered Zymomonas mobilis for salt tolerance using EZ-Tn5-based transposon insertion mutagenesis system. Microb Cell Fact (2016) 0.76

Regulatory cascade and biological activity of Beauveria bassiana oosporein that limits bacterial growth after host death. Proc Natl Acad Sci U S A (2017) 0.75

Heterologous expression of the Monilinia fructicola CYP51 (MfCYP51) gene in Pichia pastoris confirms the mode of action of the novel fungicide, SYP-Z048. Front Microbiol (2015) 0.75

Partially overlapping primer-based PCR for genome walking. PLoS One (2015) 0.75

Cloning and characterization of genes involved in nostoxanthin biosynthesis of Sphingomonas elodea ATCC 31461. PLoS One (2012) 0.75

Identification of novel loci for the generation of reporter mice. Nucleic Acids Res (2016) 0.75

Molecular Cloning and Characterization of Four Genes Encoding Ethylene Receptors Associated with Pineapple (Ananas comosus L.) Flowering. Front Plant Sci (2016) 0.75

Homologous-restraint polymerase chain reaction: an efficient and rapid protocol to clone multiple homologous genes. Curr Microbiol (2008) 0.75

Sequencing the genomic regions flanking S-linked PvGLO sequences confirms the presence of two GLO loci, one of which lies adjacent to the style-length determinant gene CYP734A50. Plant Reprod (2017) 0.75

Genetic applications of an inverse polymerase chain reaction. Genetics (1988) 18.48

Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J (1995) 10.68

A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res (1988) 10.12

An improved PCR method for walking in uncloned genomic DNA. Nucleic Acids Res (1995) 8.66

A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones. Nucleic Acids Res (1990) 8.52

Activation tagging in Arabidopsis. Plant Physiol (2000) 7.09

Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics (1995) 7.08

Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool. Microbiol Mol Biol Rev (2003) 4.36

Amplification of cDNA ends based on template-switching effect and step-out PCR. Nucleic Acids Res (1999) 3.70

Genomic walking and sequencing by oligo-cassette mediated polymerase chain reaction. Nucleic Acids Res (1990) 2.69

Use of yeast artificial chromosome clones for mapping and walking within human chromosome segment 18q21.3. Proc Natl Acad Sci U S A (1989) 1.95

Genome walking with 2- to 4-kb steps using panhandle PCR. PCR Methods Appl (1993) 1.72

Agrobacterium T-DNA integration: molecules and models. Trends Genet (2004) 1.60

Full-length cDNA cloning and determination of mRNA 5' and 3' ends by amplification of adaptor-ligated cDNA. Biotechniques (1996) 1.52

High-throughput TAIL-PCR as a tool to identify DNA flanking insertions. Methods Mol Biol (2003) 1.51

Sequence specific generation of a DNA panhandle permits PCR amplification of unknown flanking DNA. Nucleic Acids Res (1992) 1.46

T-linker-specific ligation PCR (T-linker PCR): an advanced PCR technique for chromosome walking or for isolation of tagged DNA ends. Nucleic Acids Res (2003) 1.34

Rapid isolation of promoter sequences by TAIL-PCR: the 5'-flanking regions of Pal and Pgi genes from yams (Dioscorea). Mol Gen Genet (2000) 1.15

Ligation-mediated PCR for quantitative in vivo footprinting. Nat Biotechnol (2000) 1.09

Cloning and direct sequencing of plant promoters using primer-adapter mediated PCR on DNA coupled to a magnetic solid phase. Biotechniques (1992) 0.96

Single oligonucleotide nested PCR: a rapid method for the isolation of genes and their flanking regions from expressed sequence tags. Curr Genet (2004) 0.95

An inverse PCR technique to rapidly isolate the flanking DNA of dictyostelium insertion mutants. Mol Biotechnol (2004) 0.94

Direct sequencing of bacterial artificial chromosomes (BACs) and prokaryotic genomes by biotin-capture PCR. J Biotechnol (1998) 0.94

A rapid genomic walking technique based on ligation-mediated PCR and magnetic separation technology. Biotechniques (1994) 0.91

PCR walking from microdissection clone M54 identifies three exons from the human gene for the neural cell adhesion molecule L1 (CAM-L1). Nucleic Acids Res (1991) 0.87

An efficient technique for obtaining sequences flanking inserted retroviruses. Genet Anal Tech Appl (1991) 0.83

A "step down" PCR-based technique for walking into and the subsequent direct-sequence analysis of flanking genomic DNA. Methods Mol Biol (2002) 0.81

T-DNA activation tagging. Methods Mol Biol (2003) 0.80

Reconstitution of 5'-directed human mismatch repair in a purified system. Cell (2005) 3.13

G9a and Glp methylate lysine 373 in the tumor suppressor p53. J Biol Chem (2010) 2.64

An indole-3-acetic acid carboxyl methyltransferase regulates Arabidopsis leaf development. Plant Cell (2005) 1.53

Genomic evidence for COP1 as a repressor of light-regulated gene expression and development in Arabidopsis. Plant Cell (2002) 1.52

A genomewide study identifies the Wnt signaling pathway as a major target of p53 in murine embryonic stem cells. Proc Natl Acad Sci U S A (2009) 1.41

Disruption of phytoene desaturase gene results in albino and dwarf phenotypes in Arabidopsis by impairing chlorophyll, carotenoid, and gibberellin biosynthesis. Cell Res (2007) 1.32

A genome-wide analysis of blue-light regulation of Arabidopsis transcription factor gene expression during seedling development. Plant Physiol (2003) 1.27

A subgroup of MYB transcription factor genes undergoes highly conserved alternative splicing in Arabidopsis and rice. J Exp Bot (2006) 1.23

Borrelia burgdorferi small lipoprotein Lp6.6 is a member of multiple protein complexes in the outer membrane and facilitates pathogen transmission from ticks to mice. Mol Microbiol (2009) 1.12

Constitutive expression of CIR1 (RVE2) affects several circadian-regulated processes and seed germination in Arabidopsis. Plant J (2007) 1.11

hMRE11 deficiency leads to microsatellite instability and defective DNA mismatch repair. EMBO Rep (2005) 1.06

Borrelia burgdorferi complement regulator-acquiring surface protein 2 does not contribute to complement resistance or host infectivity. PLoS One (2008) 1.05

ABI4 activates DGAT1 expression in Arabidopsis seedlings during nitrogen deficiency. Plant Physiol (2011) 1.02

Molecular cloning and functional analysis of a novel type of Bowman-Birk inhibitor gene family in rice. Plant Physiol (2003) 1.00

SPOROCYTELESS modulates YUCCA expression to regulate the development of lateral organs in Arabidopsis. New Phytol (2008) 0.99

Arabidopsis RAP2.2 plays an important role in plant resistance to Botrytis cinerea and ethylene responses. New Phytol (2012) 0.99

An arabidopsis promoter microarray and its initial usage in the identification of HY5 binding targets in vitro. Plant Mol Biol (2004) 0.98

Arabidopsis AtBECLIN 1/AtAtg6/AtVps30 is essential for pollen germination and plant development. Cell Res (2007) 0.98

A gain-of-function mutation of transcriptional factor PTL results in curly leaves, dwarfism and male sterility by affecting auxin homeostasis. Plant Mol Biol (2007) 0.94

Two groups of MYB transcription factors share a motif which enhances trans-activation activity. Biochem Biophys Res Commun (2006) 0.94

BCR-ABL1 mediates up-regulation of Fyn in chronic myelogenous leukemia. Blood (2008) 0.94

Identification and characterization of COI1-dependent transcription factor genes involved in JA-mediated response to wounding in Arabidopsis plants. Plant Cell Rep (2007) 0.92

Transcriptional profiling of rice early response to Magnaporthe oryzae identified OsWRKYs as important regulators in rice blast resistance. PLoS One (2013) 0.91

Regulation of replication protein A functions in DNA mismatch repair by phosphorylation. J Biol Chem (2006) 0.91

Proteolysis of BB0323 results in two polypeptides that impact physiologic and infectious phenotypes in Borrelia burgdorferi. Mol Microbiol (2013) 0.91

Candidate dietary phytochemicals modulate expression of phase II enzymes GSTP1 and NQO1 in human lung cells. J Nutr (2010) 0.91

Arabidopsis FHY1 protein stability is regulated by light via phytochrome A and 26S proteasome. Plant Physiol (2005) 0.90

Genomic analysis of freshwater cyanophage Pf-WMP3 Infecting cyanobacterium Phormidium foveolarum: the conserved elements for a phage. Microb Ecol (2008) 0.89

Cyanophage Pf-WMP4, a T7-like phage infecting the freshwater cyanobacterium Phormidium foveolarum: complete genome sequence and DNA translocation. Virology (2007) 0.88

dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary. Dev Biol (2013) 0.88

Activation of JNK signaling links lgl mutations to disruption of the cell polarity and epithelial organization in Drosophila imaginal discs. Cell Res (2010) 0.87

The LSD1-interacting protein GILP is a LITAF domain protein that negatively regulates hypersensitive cell death in Arabidopsis. PLoS One (2011) 0.86

Meta-analysis of 125 rheumatoid arthritis-related single nucleotide polymorphisms studied in the past two decades. PLoS One (2012) 0.86

A novel bacterium Saprospira sp. strain PdY3 forms bundles and lyses cyanobacteria. Front Biosci (2006) 0.86

Hypoxia-inducible factor-1α overexpression indicates poor clinical outcomes in tongue squamous cell carcinoma. Exp Ther Med (2012) 0.84

Single-molecule detection of proteins using aptamer-functionalized molecular electronic devices. Angew Chem Int Ed Engl (2011) 0.84

ABA-insensitive (ABI) 4 and ABI5 synergistically regulate DGAT1 expression in Arabidopsis seedlings under stress. FEBS Lett (2013) 0.84

Over-expression of WOX1 leads to defects in meristem development and polyamine homeostasis in Arabidopsis. J Integr Plant Biol (2011) 0.84

Identification of a bHLH-type G-box binding factor and its regulation activity with G-box and Box I elements of the PsCHS1 promoter. Plant Cell Rep (2006) 0.83

AtCDC5 regulates the G2 to M transition of the cell cycle and is critical for the function of Arabidopsis shoot apical meristem. Cell Res (2007) 0.83

The Arabidopsis APC4 subunit of the anaphase-promoting complex/cyclosome (APC/C) is critical for both female gametogenesis and embryogenesis. Plant J (2011) 0.83

The LSD1-type zinc finger motifs of Pisum sativa LSD1 are a novel nuclear localization signal and interact with importin alpha. PLoS One (2011) 0.82

Cadmium resistance in transgenic tobacco plants enhanced by expressing bean heavy metal-responsive gene PvSR2. Sci China C Life Sci (2003) 0.82

Expression and purification of Huwentoxin-I in baculovirus system. Protein Expr Purif (2005) 0.82

Two novel RING-type ubiquitin ligases, RGLG3 and RGLG4, are essential for jasmonate-mediated responses in Arabidopsis. Plant Physiol (2012) 0.82

TYchi, a novel chitinase with RNA N-glycosidase and anti-tumor activities. Front Biosci (2008) 0.81

AtMYB14 Regulates Cold Tolerance in Arabidopsis. Plant Mol Biol Report (2013) 0.81

Directing silicon-graphene self-assembly as a core/shell anode for high-performance lithium-ion batteries. Langmuir (2013) 0.81

Mapping of Cu and Pb contaminations in soil using combined geochemistry, topography, and remote sensing: a case study in the Le'an River floodplain, China. Int J Environ Res Public Health (2012) 0.81

High-throughput library screening identifies two novel NQO1 inducers in human lung cells. Am J Respir Cell Mol Biol (2011) 0.80

Overexpression of constitutively active OsCPK10 increases Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea. Plant Physiol Biochem (2013) 0.80

Inhibition of the NADPH oxidase regulates heme oxygenase 1 expression in chronic myeloid leukemia. Cancer (2011) 0.80

Expression and activity of Fyn mediate proliferation and blastic features of chronic myelogenous leukemia. PLoS One (2012) 0.80

Triton, a novel family of miniature inverted-repeat transposable elements (MITEs) in Trichosanthes kirilowii Maximowicz and its effect on gene regulation. Biochem Biophys Res Commun (2007) 0.79

A nuclear-encoded mitochondrial gene AtCIB22 is essential for plant development in Arabidopsis. J Genet Genomics (2010) 0.79

[Hypocholesterolemic effect of total isoflavones from Pueraria lobata in ovariectomized rats]. Zhong Yao Cai (2002) 0.78

Genome-wide haplotype association study identifies BLM as a risk gene for prostate cancer in Chinese population. Tumour Biol (2014) 0.78

Characterization of sequence elements from Malvastrum yellow vein betasatellite regulating promoter activity and DNA replication. Virol J (2012) 0.78

Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis. BMC Dev Biol (2009) 0.78

Soluble expression and one-step purification of a neurotoxin Huwentoxin-I in Escherichia coli. Protein Expr Purif (2009) 0.78

Enhanced stability of (111)-surface-dominant core-shell nanoparticle catalysts towards the oxygen reduction reaction. ChemSusChem (2013) 0.78

Brain-network mechanisms underlying the divergent effects of depression on spontaneous versus evoked pain in rats: a multiple single-unit study. Exp Neurol (2013) 0.78

Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter. Sci China C Life Sci (2009) 0.78

Cloning and expression analysis of Zmglp1, a new germin-like protein gene in maize. Biochem Biophys Res Commun (2005) 0.78

[Estrogen-like effects of puerarin and total isoflavones from Pueraria lobata]. Zhong Yao Cai (2002) 0.77

Prioritization of rheumatoid arthritis risk subpathways based on global immune subpathway interaction network and random walk strategy. Mol Biosyst (2015) 0.77

Determination of Fatty Acid Metabolism with Dynamic [11C]Palmitate Positron Emission Tomography of Mouse Heart In Vivo. Mol Imaging (2015) 0.77

Design of functionalized cellulosic honeycomb films: site-specific biomolecule modification via "click chemistry". Biomacromolecules (2012) 0.77

HGPGD: the human gene population genetic difference database. PLoS One (2013) 0.77

Virus induced gene silencing of AtCDC5 results in accelerated cell death in Arabidopsis leaves. Plant Physiol Biochem (2006) 0.77

Oral immunisation of mice with a recombinant rabies virus vaccine incorporating the heat-labile enterotoxin B subunit of Escherichia coli in an attenuated Salmonella strain. Res Vet Sci (2011) 0.77

Enhanced green fluorescence protein tracks trichosanthin in human choriocarcinoma cells as a feasible and stable reporter. Front Biosci (2005) 0.77

Trichomislin, a novel ribosome-inactivating protein, induces apoptosis that involves mitochondria and caspase-3. Arch Biochem Biophys (2005) 0.77

A novel antimicrobial protein isolated from potato (Solanum tuberosum) shares homology with an acid phosphatase. Biochem J (2003) 0.77

[Protective effect of total isoflavones from Pueraria lobata on secondary osteoporosis induced by dexamethasone in rats]. Zhong Yao Cai (2002) 0.76

Deferoxamine promotes MDA-MB-231 cell migration and invasion through increased ROS-dependent HIF-1α accumulation. Cell Physiol Biochem (2014) 0.76

One-step preparation of macroporous polymer particles with multiple interconnected chambers: a candidate for trapping biomacromolecules. Angew Chem Int Ed Engl (2013) 0.75

RGLG3 and RGLG4, novel ubiquitin ligases modulating jasmonate signaling. Plant Signal Behav (2012) 0.75

Overproduction of OsSLRL2 alters the development of transgenic Arabidopsis plants. Biochem Biophys Res Commun (2007) 0.75

I-PfoP3I: a novel nicking HNH homing endonuclease encoded in the group I intron of the DNA polymerase gene in Phormidium foveolarum phage Pf-WMP3. PLoS One (2012) 0.75

Nitrogen deficiency system is helpful in characterizing regulation mechanisms of ectopic triacylglycerol accumulation in Arabidopsis seedlings. Plant Signal Behav (2011) 0.75

Solution structure of LCI, a novel antimicrobial peptide from Bacillus subtilis. Biochemistry (2011) 0.75

Structure basis for the unique specificity of medaka enteropeptidase light chain. Protein Cell (2014) 0.75

Comparing the evolutionary conservation between human essential genes, human orthologs of mouse essential genes and human housekeeping genes. Brief Bioinform (2015) 0.75

[The expression and clinical significance of pepsin and pepsinogen in patients with otitis media with effusion]. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi (2015) 0.75