Atomic force microscopy reveals the stoichiometry and subunit arrangement of 5-HT3 receptors.

PubWeight™: 1.52‹?› | Rank: Top 4%

🔗 View Article (PMC 1194916)

Published in Proc Natl Acad Sci U S A on August 22, 2005

Authors

Nelson P Barrera1, Paul Herbert, Robert M Henderson, Ian L Martin, J Michael Edwardson

Author Affiliations

1: Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, United Kingdom.

Articles citing this

The Concise Guide to PHARMACOLOGY 2013/14: ligand-gated ion channels. Br J Pharmacol (2013) 1.88

5-HT3 receptors. Curr Pharm Des (2006) 1.49

Biophysics of P2X receptors. Pflugers Arch (2006) 1.35

Cysteine modification reveals which subunits form the ligand binding site in human heteromeric 5-HT3AB receptors. J Physiol (2011) 1.29

The transient receptor potential channels TRPP2 and TRPC1 form a heterotetramer with a 2:2 stoichiometry and an alternating subunit arrangement. J Biol Chem (2009) 1.17

The multimeric structure of polycystin-2 (TRPP2): structural-functional correlates of homo- and hetero-multimers with TRPC1. Hum Mol Genet (2009) 1.17

3B but which 3B and that's just one of the questions: the heterogeneity of human 5-HT3 receptors. Trends Pharmacol Sci (2008) 1.14

5-HT(3) receptors. J Biol Chem (2012) 1.11

Binding sites for bilobalide, diltiazem, ginkgolide, and picrotoxinin at the 5-HT3 receptor. Mol Pharmacol (2011) 1.10

High-frequency HTR3B variant associated with major depression dramatically augments the signaling of the human 5-HT3AB receptor. Proc Natl Acad Sci U S A (2008) 1.09

Agonists and antagonists bind to an A-A interface in the heteromeric 5-HT3AB receptor. Biophys J (2010) 1.08

Structural determinants of Ca2+ permeability and conduction in the human 5-hydroxytryptamine type 3A receptor. J Biol Chem (2008) 1.07

Demonstration of a direct interaction between sigma-1 receptors and acid-sensing ion channels. Biophys J (2010) 1.06

Atomic force microscopy reveals the alternating subunit arrangement of the TRPP2-TRPV4 heterotetramer. Biophys J (2010) 1.01

VUF10166, a novel compound with differing activities at 5-HT₃A and 5-HT₃AB receptors. J Pharmacol Exp Ther (2012) 0.95

Detection of human and rodent 5-HT3B receptor subunits by anti-peptide polyclonal antibodies. BMC Neurosci (2006) 0.95

The stoichiometry of P2X2/6 receptor heteromers depends on relative subunit expression levels. Biophys J (2007) 0.92

Transient expression of functional serotonin 5-HT3 receptors by glutamatergic granule cells in the early postnatal mouse cerebellum. J Physiol (2011) 0.91

The 5-HT3AB receptor shows an A3B2 stoichiometry at the plasma membrane. Biophys J (2013) 0.91

On the voltage-dependent Ca2+ block of serotonin 5-HT3 receptors: a critical role of intracellular phosphates. J Physiol (2008) 0.88

Tandem couture: Cys-loop receptor concatamer insights and caveats. Mol Neurobiol (2007) 0.87

Discriminating between 5-HT₃A and 5-HT₃AB receptors. Br J Pharmacol (2013) 0.87

5-Fluorotryptamine is a partial agonist at 5-HT3 receptors, and reveals that size and electronegativity at the 5 position of tryptamine are critical for efficient receptor function. Eur J Pharmacol (2007) 0.86

Design, synthesis, and structure-activity relationships of highly potent 5-HT₃ receptor ligands. J Med Chem (2012) 0.85

Immunomodulatory effects mediated by serotonin. J Immunol Res (2015) 0.85

The Kv7.2/Kv7.3 heterotetramer assembles with a random subunit arrangement. J Biol Chem (2012) 0.85

Discovery of a novel allosteric modulator of 5-HT3 receptors: inhibition and potentiation of Cys-loop receptor signaling through a conserved transmembrane intersubunit site. J Biol Chem (2012) 0.85

Manipulation of Subunit Stoichiometry in Heteromeric Membrane Proteins. Structure (2016) 0.85

α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptors adopt different subunit arrangements. J Biol Chem (2013) 0.84

Determination of the architecture of ionotropic receptors using AFM imaging. Pflugers Arch (2007) 0.84

Hydrophobic photolabeling studies identify the lipid-protein interface of the 5-HT3A receptor. Biochemistry (2009) 0.80

Single protein molecule mapping with magnetic atomic force microscopy. Biophys J (2010) 0.77

2Bs or not 2Bs: that is the question. J Physiol (2011) 0.75

Exploring a potential palonosetron allosteric binding site in the 5-HT(3) receptor. Bioorg Med Chem (2013) 0.75

Genotype frequencies for polymorphisms related to chemotherapy-induced nausea and vomiting in a Japanese population. J Pharm Health Care Sci (2016) 0.75

Approaching the 5-HT₃ receptor heterogeneity by computational studies of the transmembrane and intracellular domains. J Comput Aided Mol Des (2013) 0.75

Serotonergic Control of Metabolic Homeostasis. Front Cell Neurosci (2017) 0.75

Articles cited by this

Refined structure of the nicotinic acetylcholine receptor at 4A resolution. J Mol Biol (2005) 12.16

Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors. Nature (2001) 11.78

Nicotine and carbamylcholine binding to nicotinic acetylcholine receptors as studied in AChBP crystal structures. Neuron (2004) 7.48

Emerging structure of the nicotinic acetylcholine receptors. Nat Rev Neurosci (2002) 5.87

Nicotinic receptors at the amino acid level. Annu Rev Pharmacol Toxicol (2000) 4.04

Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel. Science (1991) 3.73

Cys-loop receptors: new twists and turns. Trends Neurosci (2004) 3.58

Nicotinic acetylcholine receptor at 4.6 A resolution: transverse tunnels in the channel wall. J Mol Biol (1999) 3.24

The 5-HT3B subunit is a major determinant of serotonin-receptor function. Nature (1999) 2.91

A cytoplasmic region determines single-channel conductance in 5-HT3 receptors. Nature (2003) 2.47

Forced subunit assembly in alpha1beta2gamma2 GABAA receptors. Insight into the absolute arrangement. J Biol Chem (2002) 2.44

5-HT3 receptors are membrane ion channels. Nature (1989) 2.21

Molecular weight in detergent solution of acetylcholine receptor from Torpedo californica. Biochemistry (1978) 2.08

Atomic force microscopy imaging demonstrates that P2X2 receptors are trimers but that P2X6 receptor subunits do not oligomerize. J Biol Chem (2005) 1.84

Assembly and cell surface expression of heteromeric and homomeric gamma-aminobutyric acid type A receptors. J Biol Chem (1996) 1.82

Molecular weights of individual proteins correlate with molecular volumes measured by atomic force microscopy. Pflugers Arch (1998) 1.82

Cloning, physical mapping and expression analysis of the human 5-HT3 serotonin receptor-like genes HTR3C, HTR3D and HTR3E. Gene (2003) 1.67

The pharmacological and functional characteristics of the serotonin 5-HT(3A) receptor are specifically modified by a 5-HT(3B) receptor subunit. J Biol Chem (1999) 1.62

Stoichiometry of a ligand-gated ion channel determined by fluorescence energy transfer. J Biol Chem (1999) 1.41

Formation of the alpha-bungarotoxin binding site and assembly of the nicotinic acetylcholine receptor subunits occur in the endoplasmic reticulum. J Biol Chem (1987) 1.39

The arrangement of the subunits of the acetylcholine receptor of Torpedo californica. J Biol Chem (1983) 1.30

Differential composition of 5-hydroxytryptamine3 receptors synthesized in the rat CNS and peripheral nervous system. J Neurosci (2002) 1.29

The Ligand Gated Ion Channel Database. Nucleic Acids Res (1999) 1.26

Functional properties of a cloned 5-hydroxytryptamine ionotropic receptor subunit: comparison with native mouse receptors. J Physiol (1994) 1.26

Acetylcholine receptor assembly: subunit folding and oligomerization occur sequentially. Cell (1993) 1.25

5-HT3 receptor channels in dissociated rat superior cervical ganglion neurons. J Physiol (1992) 1.24

The medical benefit of 5-HT research. Pharmacol Biochem Behav (2002) 1.23

Prediction of 5-HT3 receptor agonist-binding residues using homology modeling. Biophys J (2003) 1.22

Pharmacological comparison of human homomeric 5-HT3A receptors versus heteromeric 5-HT3A/3B receptors. Neuropharmacology (2001) 1.17

Ultrastructure of the 5-hydroxytryptamine3 receptor. J Neurochem (1995) 1.08

Assembly and cell surface expression of homomeric and heteromeric 5-HT3 receptors: the role of oligomerization and chaperone proteins. Mol Cell Neurosci (2002) 1.07

The 5-HT3B subunit confers reduced sensitivity to picrotoxin when co-expressed with the 5-HT3A receptor. Brain Res Mol Brain Res (2003) 0.96

5-HT(3)-receptor subunits A and B are co-expressed in neurons of the dorsal root ganglion. J Comp Neurol (2001) 0.93

Identification and importance of N-glycosylation of the human 5-hydroxytryptamine3A receptor subunit. Biochem Pharmacol (2004) 0.92

Single channel properties of the 5-HT3 subtype of serotonin receptor in primary cultures of rodent hippocampus. Neurosci Lett (1994) 0.92

The dielectric method of estimating protein hydration. Phys Med Biol (1957) 0.89

Molecular properties of 5-hydroxytryptamine3 receptor-type binding sites purified from NG108-15 cells. J Neurochem (1992) 0.80

Articles by these authors

Atomic force microscopy imaging demonstrates that P2X2 receptors are trimers but that P2X6 receptor subunits do not oligomerize. J Biol Chem (2005) 1.84

Encapsulation of exenatide in poly-(D,L-lactide-co-glycolide) microspheres produced an investigational long-acting once-weekly formulation for type 2 diabetes. Diabetes Technol Ther (2011) 1.57

Compound exocytosis: mechanisms and functional significance. Traffic (2006) 1.56

Real-time analysis of the effects of cholesterol on lipid raft behavior using atomic force microscopy. Biophys J (2003) 1.53

Identification of synaptotagmin effectors via acute inhibition of secretion from cracked PC12 cells. J Cell Biol (2003) 1.51

Cryoelectron tomographic analysis of an HIV-neutralizing protein and its complex with native viral gp120. J Biol Chem (2007) 1.48

Fast-scan atomic force microscopy reveals that the type III restriction enzyme EcoP15I is capable of DNA translocation and looping. Proc Natl Acad Sci U S A (2007) 1.31

DNA looping and translocation provide an optimal cleavage mechanism for the type III restriction enzymes. EMBO J (2007) 1.28

Placental alkaline phosphatase is efficiently targeted to rafts in supported lipid bilayers. J Biol Chem (2002) 1.27

Analysis of assembly and trafficking of native P2X4 and P2X7 receptor complexes in rodent immune cells. J Biol Chem (2009) 1.26

Atomic force microscopy reveals the stoichiometry and subunit arrangement of the alpha4beta3delta GABA(A) receptor. Mol Pharmacol (2007) 1.21

Association of the endosomal sorting complex ESCRT-II with the Vps20 subunit of ESCRT-III generates a curvature-sensitive complex capable of nucleating ESCRT-III filaments. J Biol Chem (2011) 1.18

Dynamic regulation of the large exocytotic fusion pore in pancreatic acinar cells. Mol Biol Cell (2007) 1.18

The transient receptor potential channels TRPP2 and TRPC1 form a heterotetramer with a 2:2 stoichiometry and an alternating subunit arrangement. J Biol Chem (2009) 1.17

Atomic force microscopy of the EcoKI Type I DNA restriction enzyme bound to DNA shows enzyme dimerization and DNA looping. Nucleic Acids Res (2009) 1.17

Direct imaging shows that insulin granule exocytosis occurs by complete vesicle fusion. Proc Natl Acad Sci U S A (2004) 1.14

The plasma membrane Q-SNARE syntaxin 2 enters the zymogen granule membrane during exocytosis in the pancreatic acinar cell. J Biol Chem (2004) 1.11

Atomic force microscopy reveals the architecture of the epithelial sodium channel (ENaC). J Biol Chem (2011) 1.10

Direct visualization of the trimeric structure of the ASIC1a channel, using AFM imaging. Biochem Biophys Res Commun (2008) 1.08

Demonstration of a direct interaction between sigma-1 receptors and acid-sensing ion channels. Biophys J (2010) 1.06

Receptor binding enables botulinum neurotoxin B to sense low pH for translocation channel assembly. Cell Host Microbe (2011) 1.06

Direct evidence for functional TRPV1/TRPA1 heteromers. Pflugers Arch (2014) 1.03

Direct visualization of ligand-protein interactions using atomic force microscopy. Br J Pharmacol (2002) 1.03

The regulation of exocytosis in the pancreatic acinar cell. Cell Signal (2002) 1.03

The influence of DNA stiffness upon nucleosome formation. J Struct Biol (2004) 1.02

ESCRT-0 assembles as a heterotetrameric complex on membranes and binds multiple ubiquitinylated cargoes simultaneously. J Biol Chem (2010) 1.01

An alternating GluN1-2-1-2 subunit arrangement in mature NMDA receptors. PLoS One (2012) 1.01

Atomic force microscopy reveals the alternating subunit arrangement of the TRPP2-TRPV4 heterotetramer. Biophys J (2010) 1.01

Identification of SNAREs that mediate zymogen granule exocytosis. Biochem Biophys Res Commun (2007) 0.99

An uncharged region within the N terminus of the P2X6 receptor inhibits its assembly and exit from the endoplasmic reticulum. Mol Pharmacol (2006) 0.98

AFM imaging reveals the tetrameric structure of the TRPC1 channel. Biochem Biophys Res Commun (2007) 0.97

Direct visualization of G-quadruplexes in DNA using atomic force microscopy. Nucleic Acids Res (2009) 0.97

Isomerization of the proline in the M2-M3 linker is not required for activation of the human 5-HT3A receptor. J Neurochem (2009) 0.94

Synaptotagmin perturbs the structure of phospholipid bilayers. Biochemistry (2008) 0.92

The sigma-1 receptor binds to the Nav1.5 voltage-gated Na+ channel with 4-fold symmetry. J Biol Chem (2012) 0.92

The stoichiometry of P2X2/6 receptor heteromers depends on relative subunit expression levels. Biophys J (2007) 0.92

Structure and binding mechanism of vascular endothelial cadherin: a divergent classical cadherin. J Mol Biol (2011) 0.91

Targeting of Helicobacter pylori vacuolating toxin to lipid raft membrane domains analysed by atomic force microscopy. Biochem J (2004) 0.91

The subunit arrangement and assembly of ionotropic receptors. Trends Neurosci (2008) 0.90

Lipid rafts: feeling is believing. News Physiol Sci (2004) 0.90

Crystal structure and molecular imaging of the Nav channel β3 subunit indicates a trimeric assembly. J Biol Chem (2014) 0.90

The σ-1 receptor interacts directly with GluN1 but not GluN2A in the GluN1/GluN2A NMDA receptor. J Neurosci (2013) 0.89

DNA translocation by type III restriction enzymes: a comparison of current models of their operation derived from ensemble and single-molecule measurements. Nucleic Acids Res (2011) 0.89

AFM imaging reveals the tetrameric structure of the TRPM8 channel. Biochem Biophys Res Commun (2010) 0.89

Analysis of naturally occurring mutations in the human lipodystrophy protein seipin reveals multiple potential pathogenic mechanisms. Diabetologia (2013) 0.88

Unusual structures are present in DNA fragments containing super-long Huntingtin CAG repeats. PLoS One (2011) 0.87

Synthetic self-assembling clostridial chimera for modulation of sensory functions. Bioconjug Chem (2013) 0.85

Interaction of syncollin with GP-2, the major membrane protein of pancreatic zymogen granules, and association with lipid microdomains. Biochem J (2002) 0.85

The Kv7.2/Kv7.3 heterotetramer assembles with a random subunit arrangement. J Biol Chem (2012) 0.85

α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptors adopt different subunit arrangements. J Biol Chem (2013) 0.84

Determination of the architecture of ionotropic receptors using AFM imaging. Pflugers Arch (2007) 0.84

Atomic force microscopy study of the structural effects induced by echinomycin binding to DNA. J Mol Biol (2005) 0.83

Myelin basic protein and myelin protein 2 act synergistically to cause stacking of lipid bilayers. Biochemistry (2010) 0.83

Atomic force microscopy and drug discovery. Drug Discov Today (2004) 0.83

Effects of synaptotagmin reveal two distinct mechanisms of agonist-stimulated internalization of the M4 muscarinic acetylcholine receptor. Br J Pharmacol (2005) 0.83

Syncollin inhibits regulated corticotropin secretion from AtT-20 cells through a reduction in the secretory vesicle population. Biochem J (2004) 0.83

Syncollin is required for efficient zymogen granule exocytosis. Biochem J (2005) 0.83

Visual analysis of concerted cleavage by type IIF restriction enzyme SfiI in subsecond time region. Biophys J (2011) 0.83

Post-testicular development of a novel membrane substructure within the equatorial segment of ram, bull, boar, and goat spermatozoa as viewed by atomic force microscopy. J Struct Biol (2002) 0.83

Exploring ligand recognition and ion flow in comparative models of the human GABA type A receptor. J Mol Graph Model (2007) 0.82

Acetylcholine-induced zymogen granule exocytosis: comparison between acini and single pancreatic acinar cells. Pancreas (2002) 0.81

Visualization of structural changes accompanying activation of N-methyl-D-aspartate (NMDA) receptors using fast-scan atomic force microscopy imaging. J Biol Chem (2012) 0.81

Structural perturbations in DNA caused by bis-intercalation of ditercalinium visualised by atomic force microscopy. Nucleic Acids Res (2002) 0.81

The endophilin N-BAR domain perturbs the structure of lipid bilayers. Biochemistry (2010) 0.81

Organization and synergistic binding of copine I and annexin A1 on supported lipid bilayers observed by atomic force microscopy. Biochim Biophys Acta (2009) 0.80

Atomic force microscopy imaging reveals the domain structure of polycystin-1. Biochemistry (2012) 0.80

Interaction of synaptotagmin with lipid bilayers, analyzed by single-molecule force spectroscopy. Biophys J (2010) 0.80

Acid-sensing ion channel (ASIC) 1a undergoes a height transition in response to acidification. FEBS Lett (2010) 0.79

The binding mode of the DNA bisintercalator luzopeptin investigated using atomic force microscopy. J Struct Biol (2003) 0.79

Demonstration of ligand decoration, and ligand-induced perturbation, of G-quadruplexes in a plasmid using atomic force microscopy. Biochemistry (2012) 0.78

Membrane thickness changes ion-selectivity of channel-proteins. Cell Physiol Biochem (2004) 0.78

Automated analysis of the architecture of receptors, imaged by atomic force microscopy. Micron (2007) 0.77

Imaging the spatial orientation of subunits within membrane receptors by atomic force microscopy. Methods Mol Biol (2011) 0.77