Purification and Properties of a Highly Thermostable, Sodium Dodecyl Sulfate-Resistant and Stereospecific Proteinase from the Extremely Thermophilic Archaeon Thermococcus stetteri.

PubWeight™: 1.08‹?› | Rank: Top 10%

🔗 View Article (PMID 16535107)

Published in Appl Environ Microbiol on August 01, 1995

Authors

M Klingeberg, B Galunsky, C Sjoholm, V Kasche, G Antranikian

Articles citing this

Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability. Microbiol Mol Biol Rev (2001) 5.34

Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus. J Bacteriol (1996) 1.34

Keratin Degradation by Fervidobacterium pennavorans, a Novel Thermophilic Anaerobic Species of the Order Thermotogales. Appl Environ Microbiol (1996) 1.32

Characterization of native and recombinant forms of an unusual cobalt-dependent proline dipeptidase (prolidase) from the hyperthermophilic archaeon Pyrococcus furiosus. J Bacteriol (1998) 1.13

Proteolysis in hyperthermophilic microorganisms. Archaea (2002) 0.99

Biodiversity of thermophilic prokaryotes with hydrolytic activities in hot springs of Uzon Caldera, Kamchatka (Russia). Appl Environ Microbiol (2008) 0.98

Purification and Characterization of Two Functional Forms of Intracellular Protease PfpI from the Hyperthermophilic Archaeon Pyrococcus furiosus. Appl Environ Microbiol (1997) 0.96

A novel extracellular subtilisin-like protease from the hyperthermophile Aeropyrum pernix K1: biochemical properties, cloning, and expression. Extremophiles (2003) 0.89

Thermal adaptation of dihydrofolate reductase from the moderate thermophile Geobacillus stearothermophilus. Biochemistry (2014) 0.75

Characterization of an extracellular alkaline serine protease from marine Engyodontium album BTMFS10. J Ind Microbiol Biotechnol (2010) 0.75

New cultural approaches for microaerophilic hyperthermophiles. Curr Microbiol (2010) 0.75

Articles cited by this

Protein measurement with the Folin phenol reagent. J Biol Chem (1951) 1743.91

Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (1970) 1528.65

The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem (1969) 142.11

On the size of the active site in proteases. I. Papain. Biochem Biophys Res Commun (1967) 18.32

Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. Anal Biochem (1980) 7.23

Viologen dye inhibition of methane formation by Methanobacillus omelianskii. J Bacteriol (1964) 5.35

AN APPARATUS FOR PREPARATIVE TEMPERATURE-REGULATED POLYACRYLAMIDE GEL ELECTROPHORESIS. Anal Biochem (1964) 4.43

Comparative specificity of microbial proteinases. Adv Enzymol Relat Areas Mol Biol (1974) 2.28

Molecular weight estimations of proteins by electrophoresis in polyacrylamide gels of graded porosity. FEBS Lett (1972) 2.05

Characterization of sodium dodecyl sulfate-resistant proteolytic activity in the hyperthermophilic archaebacterium Pyrococcus furiosus. Appl Environ Microbiol (1990) 1.83

Relationship of protein flexibility to thermostability. Protein Eng (1987) 1.72

The purification and characterization of an extremely thermostable alpha-amylase from the hyperthermophilic archaebacterium Pyrococcus furiosus. J Biol Chem (1993) 1.52

Alpha-amylase from the hyperthermophilic archaebacterium Pyrococcus furiosus. Cloning and sequencing of the gene and expression in Escherichia coli. J Biol Chem (1993) 1.47

An extremely thermostable extracellular proteinase from a strain of the archaebacterium Desulfurococcus growing at 88 degrees C. Biochem J (1987) 1.45

Detection of proteases in polyacrylamide gels containing covalently bound substrates. Anal Biochem (1984) 1.08

Purification, characterization, and gene cloning of thermopsin, a thermostable acid protease from Sulfolobus acidocaldarius. J Biol Chem (1990) 1.05

A perspective on the biotechnological potential of extremophiles. Trends Biotechnol (1992) 1.04

Pore gradient electrophoresis. Anal Biochem (1971) 1.04

The effect of temperature on the fluorescence of some aromatic amino acids and proteins. Biochim Biophys Acta (1962) 1.02

A heat-stable serine proteinase from the extreme thermophilic archaebacterium Sulfolobus solfataricus. Biochim Biophys Acta (1992) 1.01

Determination of the individual rate constants of alpha-chymotrypsin-catalyzed hydrolysis with the added nucleophilic agent, 1,4-butanediol. FEBS Lett (1971) 0.93

Biotechnology of the Archaea. Trends Biotechnol (1992) 0.88

Catalytic properties of serine proteases. 2. Comparison between human urinary kallikrein and human urokinase, bovine beta-trypsin, bovine thrombin, and bovine alpha-chymotrypsin. Biochemistry (1982) 0.88

Autolysis of beta-trypsin. Influence of calcium ions and heat. Acta Chem Scand (1973) 0.84

Articles by these authors

The kinetics of phytochrome conversion. J Biol Chem (1966) 4.05

Extremophiles as a source of novel enzymes for industrial application. Appl Microbiol Biotechnol (1999) 2.33

Xylanolytic enzymes from fungi and bacteria. Crit Rev Biotechnol (1997) 2.16

Low-temperature studies on phytochrome: light and dark reactions in the red to far-red transformation and new intermediate forms of phytochrome. Proc Natl Acad Sci U S A (1968) 2.05

KINETICS OF PHYTOCHROME CONVERSION: MULTIPLE PATHWAYS IN THE P(r) TO P(fr) REACTION, AS STUDIED BY DOUBLE-FLASH TECHNIQUE. Proc Natl Acad Sci U S A (1967) 1.96

Thermostable amylolytic enzymes from a new clostridium isolate. Appl Environ Microbiol (1987) 1.83

Genome sequence of Picrophilus torridus and its implications for life around pH 0. Proc Natl Acad Sci U S A (2004) 1.51

Structure of a slow processing precursor penicillin acylase from Escherichia coli reveals the linker peptide blocking the active-site cleft. J Mol Biol (2000) 1.41

Characterization of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530. Appl Environ Microbiol (1995) 1.35

Keratin Degradation by Fervidobacterium pennavorans, a Novel Thermophilic Anaerobic Species of the Order Thermotogales. Appl Environ Microbiol (1996) 1.32

Enzymatic production of cyclodextrins. Appl Microbiol Biotechnol (2002) 1.28

Proteins from hyperthermophiles: stability and enzymatic catalysis close to the boiling point of water. Adv Biochem Eng Biotechnol (1998) 1.23

alpha-Amylase of Clostridium thermosulfurogenes EM1: nucleotide sequence of the gene, processing of the enzyme, and comparison of other alpha-amylases. Appl Environ Microbiol (1991) 1.18

Characterization of ATP citrate lyase from Chlorobium limicola. J Bacteriol (1982) 1.17

Heat-stable enzymes from extremely thermophilic and hyperthermophilic microorganisms. World J Microbiol Biotechnol (1995) 1.16

Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli. Appl Environ Microbiol (1995) 1.16

Cloning, sequencing, characterization, and expression of an extracellular alpha-amylase from the hyperthermophilic archaeon Pyrococcus furiosus in Escherichia coli and Bacillus subtilis. J Biol Chem (1997) 1.15

Glycosyl hydrolases from hyperthermophiles. Extremophiles (1997) 1.08

Immunocytochemical Identification and Localization of Active and Inactive alpha-Amylase and Pullulanase in Cells of Clostridium thermosulfurogenes EM1. Appl Environ Microbiol (1991) 1.07

Thermococcus guaymasensis sp. nov. and Thermococcus aggregans sp. nov., two novel thermophilic archaea isolated from the Guaymas Basin hydrothermal vent site. Int J Syst Bacteriol (1998) 1.03

The glutamate dehydrogenase-encoding gene of the hyperthermophilic archaeon Pyrococcus furiosus: sequence, transcription and analysis of the deduced amino acid sequence. Gene (1993) 1.03

Purification and Properties of a Thermoactive Glucoamylase from Clostridium thermosaccharolyticum. Appl Environ Microbiol (1991) 1.02

A new thermoactive pullulanase from Desulfurococcus mucosus: cloning, sequencing, purification, and characterization of the recombinant enzyme after expression in Bacillus subtilis. J Bacteriol (2000) 1.02

Thermoanaerobacter yonseiensis sp. nov., a novel extremely thermophilic, xylose-utilizing bacterium that grows at up to 85 degrees C. Int J Syst Evol Microbiol (2001) 1.02

Purification and characterization of thermostable pectate-lyases from a newly isolated thermophilic bacterium, Thermoanaerobacter italicus sp. nov. Extremophiles (1997) 1.02

Isolation of Thermoanaerobacter keratinophilus sp. nov., a novel thermophilic, anaerobic bacterium with keratinolytic activity. Extremophiles (2001) 1.00

Pullulanase type I from Fervidobacterium pennavorans Ven5: cloning, sequencing, and expression of the gene and biochemical characterization of the recombinant enzyme. Appl Environ Microbiol (1999) 0.98

Naphthalene degradation and incorporation of naphthalene-derived carbon into biomass by the thermophile Bacillus thermoleovorans. Appl Environ Microbiol (2000) 0.97

Copurification of citrate lyase and citrate lyase ligase from Rhodopseudomonas gelatinosa and subsequent separation of the two enzymes. Eur J Biochem (1982) 0.97

Immobilized enzymes: crystals or carriers? Trends Biotechnol (1999) 0.96

Characterization of the xylanases from the new isolated thermophilic xylan-degrading Bacillus thermoleovorans strain K-3d and Bacillus flavothermus strain LB3A. FEMS Microbiol Lett (1997) 0.95

Cloning, expression and biochemical characterisation of a unique thermostable pullulan-hydrolysing enzyme from the hyperthermophilic archaeon Thermococcus aggregans. FEMS Microbiol Lett (2000) 0.93

Purification and Properties of a Thermostable Pullulanase from a Newly Isolated Thermophilic Anaerobic Bacterium, Fervidobacterium pennavorans Ven5. Appl Environ Microbiol (1997) 0.93

Anaerobranca gottschalkii sp. nov., a novel thermoalkaliphilic bacterium that grows anaerobically at high pH and temperature. Int J Syst Evol Microbiol (2001) 0.93

Reevaluation of citrate lyase from Escherichia coli. Biochim Biophys Acta (1987) 0.89

Alicyclobacillus hesperidum sp. nov. and a related genomic species from solfataric soils of São Miguel in the Azores. Int J Syst Evol Microbiol (2000) 0.89

Improvement of posttranslational bottlenecks in the production of penicillin amidase in recombinant Escherichia coli strains. Appl Environ Microbiol (2003) 0.89

Production of novel pullulanases at high concentrations by two newly isolated thermophilic clostridia. FEMS Microbiol Lett (1990) 0.88

Effect of isomalt consumption on faecal microflora and colonic metabolism in healthy volunteers. Br J Nutr (2006) 0.87

Covalent modification of proteins in Bacillus subtilis during the process of sporulation, germination and outgrowth. FEMS Microbiol Lett (1989) 0.86

The membrane dialysis bioreactor with integrated radial-flow fixed bed--a new approach for continuous cultivation of animal cells. Cytotechnology (1992) 0.86

Determination of the kinetic parameters during continuous cultivation of the lipase-producing thermophile Bacillus sp. IHI-91 on olive oil. Appl Microbiol Biotechnol (1997) 0.85

Cloning and characterisation of a thermostable alpha-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY. Gene (1997) 0.85

Autolysis of beta-trypsin. Influence of calcium ions and heat. Acta Chem Scand (1973) 0.84

Cloning and characterization of a family B DNA polymerase from the hyperthermophilic crenarchaeon Pyrobaculum islandicum. J Bacteriol (2000) 0.84

Temperature effects on S1- and S'1-enantioselectivity of alpha-chymotrypsin. Biochim Biophys Acta (1997) 0.84

Sodium-coupled energy transduction in the newly isolated thermoalkaliphilic strain LBS3. J Bacteriol (1996) 0.83

Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria. J Appl Microbiol (2008) 0.82

Catalytically active monomer forms of immobilized arginase. Eur J Biochem (1983) 0.81

Direct spectrophotometric measurement of enzyme activity in heterogeneous systems with insoluble substrate or immobilized enzyme. Anal Biochem (1994) 0.81

Heat-stable pullulanase from Bacillus acidopullulyticus: characterization and refolding after guanidinium chloride-induced unfolding. Extremophiles (1999) 0.79

Characterization of citrate lyase from Clostridium sporosphaeroides. Arch Microbiol (1985) 0.79

Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOL1 and Caldanaerobacter subterraneus subsp. tengcongensis. Extremophiles (2009) 0.79

Direct measurement of pH profiles in immobilized enzyme carriers during kinetically controlled synthesis using CLSM. Biotechnol Prog (2001) 0.79

Cloning and expression of alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei in the moderately halophilic bacterium Halomonas elongata. J Appl Microbiol (2000) 0.79

Cooperative transitions between active -and -trypsin conformations. Biochem Biophys Res Commun (1972) 0.79

Crystallization and preliminary crystallographic analysis of an amylopullulanase from the hyperthermophilic archaeon Pyrococcus woesei. Proteins (1995) 0.79

Amylolytic enzymes from hyperthermophiles. Methods Enzymol (2001) 0.79

Physiological aspects involved in production of xylanolytic enzymes by deep-sea hyperthermophilic archaeon Pyrodictium abyssi. Appl Biochem Biotechnol (2001) 0.78

Crystallization and preliminary X-ray crystallographic studies of the thermoactive pullulanase type I, hydrolyzing alpha-1,6 glycosidic linkages, from Fervidobacterium pennivorans Ven5. Acta Crystallogr D Biol Crystallogr (2000) 0.78

PCR performance of the B-type DNA polymerase from the thermophilic euryarchaeon Thermococcus aggregans improved by mutations in the Y-GG/A motif. Nucleic Acids Res (2000) 0.78

Rapid zymogen activation and isolation of serine proteases from an individual mouse pancreas by affinity chromatography: genetical heterogeneity of chymotrypsins of Mus musculus. Biochim Biophys Acta (1977) 0.78

Isolation and characterization of lipid-degrading Bacillus thermoleovorans IHI-91 from an icelandic hot spring. Extremophiles (2000) 0.78

pH gradients in immobilized amidases and their influence on rates and yields of beta-lactam hydrolysis. Biotechnol Bioeng (1999) 0.78

Resolution in affinity chromatography. The effect of the heterogeneity of immobilized soybean trypsin inhibitor on the separation of pancreatic proteases. J Chromatogr (1976) 0.78

Single-step purification of a recombinant thermostable alpha-amylase after solubilization of the enzyme from insoluble aggregates. J Chromatogr B Biomed Sci Appl (2000) 0.77

Thermococcus siculi sp. nov., a novel hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent at the Mid-Okinawa Trough. Extremophiles (1999) 0.77

Penicillin amidase from E. coli. Enzyme heterogeneity and stability. Ann N Y Acad Sci (1987) 0.77

Kinetic studies on the mechanism and the specificity of peptide semisynthesis catalyzed by the serine proteases alpha-chymotrypsin and beta-trypsin. Biochem Biophys Res Commun (1984) 0.77

A new banding pattern of human chromosomes by in situ nick translation using ECO RI and biotin-dUTP. Clin Genet (1985) 0.77

Immobilization of anaerobic thermophilic bacteria for the production of cell-free thermostable alpha-amylases and pullulanases. Appl Microbiol Biotechnol (1990) 0.77

Reaction mechanism, specificity and pH-dependence of peptide synthesis catalyzed by the metalloproteinase thermolysin. Biochim Biophys Acta (1986) 0.77

Contributions to the S'-subsite specificity of papain. Biochim Biophys Acta (1992) 0.76

Peptide synthesis catalyzed by the serine proteinases chymotrypsin and trypsin. Biochim Biophys Acta (1985) 0.76

Biochemical and phylogenetic characterization of two novel deep-sea Thermococcus isolates with potentially biotechnological applications. Arch Microbiol (1997) 0.75

Stability, refolding and Ca2+ binding of pullulanase from the hyperthermophilic archaeon Pyrococcus woesei. Eur J Biochem (1999) 0.75

Practical application of different enzymes immobilized on sepabeads. Bioprocess Biosyst Eng (2008) 0.75

Radiation-induced cross-linking of alpha-chymotrypsin to DNA and an agarose gel. Int J Radiat Biol Relat Stud Phys Chem Med (1974) 0.75

Biospecific sample preparation and its application in isoelectric focusing. Biochem Biophys Res Commun (1979) 0.75

The kinetics of trypsinogen activation studied by analytical affinity chromatography. Arch Biochem Biophys (1976) 0.75

The regulation of citrate lyase by acetylation/deacetylation. Biochem Soc Trans (1982) 0.75

Enzyme catalyzed biotransformations in aqueous two-phase systems with precipitated substrate and/or product. Biotechnol Bioeng (1995) 0.75

Purification of L-glutamate-dependent citrate lyase from Clostridium sphenoides and electron microscopic analysis of citrate lyase isolated from Rhodopseudomonas gelatinosa, Streptococcus diacetilactis and C. sphenoides. Eur J Biochem (1982) 0.75

Activation and inactivation of citrate lyase ligase from Rhodopseudomonas gelatinosa. FEBS Lett (1978) 0.75

Covalent modification of citrate lyase ligase from Clostridium sphenoides by phosphorylation/dephosphorylation. Eur J Biochem (1985) 0.75

Oxygen effect: differences in radiation-induced deactivation of mobile and immobilized alpha-chymotrypsin. Radiat Environ Biophys (1974) 0.75

A theoretical model describing steady-state catalysis by enzymes immobilized in spherical gel particles. Experimental study of -chymotrypsin-sepharose. Biochem Biophys Res Commun (1971) 0.75

Conformational transitions in immobilized proteases. Methods Enzymol (1976) 0.75

The DNA, RNA and trypsin content in trypsins used in monolayer animal cell culture studies. Cell Biol Int Rep (1981) 0.75

Kinetically controlled equilibria. The perturbation of hydrolysis equilibria in reactions catalyzed by alpha-chymotrypsin immobilized on charges supports. Biochem Biophys Res Commun (1978) 0.75

Radical intermediates in the fluorescein- and eosin-photosensitized autoxidation of L-tyrosine. Photochem Photobiol (1967) 0.75

Protective effect of hop beta-acids on microbial degradation of thick juice during storage. J Appl Microbiol (2008) 0.75

Activity distributions in modified alpha-chymotrypsin. Biochem Biophys Res Commun (1970) 0.75

Evaluation of magnetic polymer micro-beads as carriers of immobilised biocatalysts for selective and stereoselective transformations. Biotechnol Lett (2004) 0.75

Ionic strength and pH effects in the kinetically controlled synthesis of benzylpenicillin by nucleophilic deacylation of free and immobilized phenyl-acetyl-penicillin amidase with 6-aminopenicillanic acid. Biochem Biophys Res Commun (1982) 0.75

An affinity chromatographic study of trypsinogen activation. Kinetic evidence for the existence of neotrypsinogen. Z Klin Chem Klin Biochem (1974) 0.75

High yield of mannosylglycerate production by upshock fermentation and bacterial milking of trehalose-deficient mutant Thermus thermophilus RQ-1. Appl Microbiol Biotechnol (2007) 0.75

Penicillin amidase-catalysed transfer of low specific acyl moiety. Synthesis of 7-benzoxazolonylacetamido desacetoxycephalosporanic acid. Enzyme Microb Technol (1992) 0.75