Reconstitution of the transition from lamellipodium to filopodium in a membrane-free system.

PubWeight™: 1.25‹?› | Rank: Top 10%

🔗 View Article (PMC 1458768)

Published in Proc Natl Acad Sci U S A on March 20, 2006

Authors

Lior Haviv1, Yifat Brill-Karniely, Rachel Mahaffy, Frederic Backouche, Avinoam Ben-Shaul, Thomas D Pollard, Anne Bernheim-Groswasser

Author Affiliations

1: Department of Chemical Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.

Articles citing this

Capping protein increases the rate of actin-based motility by promoting filament nucleation by the Arp2/3 complex. Cell (2008) 3.00

Ena/VASP proteins have an anti-capping independent function in filopodia formation. Mol Biol Cell (2007) 2.12

Mathematics of cell motility: have we got its number? J Math Biol (2008) 1.84

p120 catenin regulates lamellipodial dynamics and cell adhesion in cooperation with cortactin. Proc Natl Acad Sci U S A (2007) 1.59

Dual actin-bundling and protein kinase C-binding activities of fascin regulate carcinoma cell migration downstream of Rac and contribute to metastasis. Mol Biol Cell (2007) 1.45

Biology under construction: in vitro reconstitution of cellular function. Nat Rev Mol Cell Biol (2009) 1.44

Nucleation geometry governs ordered actin networks structures. Nat Mater (2010) 1.31

Arp2 depletion inhibits sheet-like protrusions but not linear protrusions of fibroblasts and lymphocytes. Cell Motil Cytoskeleton (2008) 1.31

Diverse roles of actin in C. elegans early embryogenesis. BMC Dev Biol (2007) 1.25

Filopodia initiation: focus on the Arp2/3 complex and formins. Cell Adh Migr (2011) 1.23

Confinement induces actin flow in a meiotic cytoplasm. Proc Natl Acad Sci U S A (2012) 1.07

Arp2/3 branched actin network mediates filopodia-like bundles formation in vitro. PLoS One (2008) 1.00

Thickness distribution of actin bundles in vitro. Eur Biophys J (2007) 0.98

Model of myosin node aggregation into a contractile ring: the effect of local alignment. J Phys Condens Matter (2011) 0.96

Actin bundling: initiation mechanisms and kinetics. Phys Rev Lett (2008) 0.89

Mechano-chemical feedbacks regulate actin mesh growth in lamellipodial protrusions. Biophys J (2010) 0.87

Accounting for diffusion in agent based models of reaction-diffusion systems with application to cytoskeletal diffusion. PLoS One (2011) 0.84

Actin filament attachments for sustained motility in vitro are maintained by filament bundling. PLoS One (2012) 0.84

Structural, mechanical, and dynamical variability of the actin cortex in living cells. Biophys J (2015) 0.81

Self-organizing actin patterns shape membrane architecture but not cell mechanics. Nat Commun (2017) 0.79

Reconstitution of actin-based motility by vasodilator-stimulated phosphoprotein (VASP) depends on the recruitment of F-actin seeds from the solution produced by cofilin. J Biol Chem (2014) 0.78

Cell type-dependent mechanisms for formin-mediated assembly of filopodia. Mol Biol Cell (2015) 0.78

Dynamic reorganization of the actin cytoskeleton. F1000Res (2015) 0.77

Quantifying a pathway: kinetic analysis of actin dendritic nucleation. Biophys J (2010) 0.77

Modeling the formation of in vitro filopodia. J Math Biol (2010) 0.76

The More the Tubular: Dynamic Bundling of Actin Filaments for Membrane Tube Formation. PLoS Comput Biol (2016) 0.75

Cytoskeletal control of B cell responses to antigens. Nat Rev Immunol (2017) 0.75

Self-organizing actin patterns shape cytoskeletal cortex organization. Commun Integr Biol (2017) 0.75

Articles cited by this

The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin. J Biol Chem (1971) 35.39

Cellular motility driven by assembly and disassembly of actin filaments. Cell (2003) 26.05

Molecular mechanisms controlling actin filament dynamics in nonmuscle cells. Annu Rev Biophys Biomol Struct (2000) 10.11

Reconstitution of actin-based motility of Listeria and Shigella using pure proteins. Nature (1999) 8.61

Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia. J Cell Biol (1999) 8.24

Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility. Cell (2002) 6.80

Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex. Curr Biol (1999) 6.24

Mechanism of filopodia initiation by reorganization of a dendritic network. J Cell Biol (2003) 5.47

Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins. Nature (2000) 4.73

Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose. J Cell Biol (1994) 4.61

Real-time measurements of actin filament polymerization by total internal reflection fluorescence microscopy. Biophys J (2004) 4.32

Influence of the C terminus of Wiskott-Aldrich syndrome protein (WASp) and the Arp2/3 complex on actin polymerization. Biochemistry (1999) 3.77

WASP and WAVE family proteins: key molecules for rapid rearrangement of cortical actin filaments and cell movement. J Cell Sci (2001) 3.74

Lamellipodial versus filopodial mode of the actin nanomachinery: pivotal role of the filament barbed end. Cell (2004) 3.48

Physical properties determining self-organization of motors and microtubules. Science (2001) 3.35

The actin-based nanomachine at the leading edge of migrating cells. Biophys J (1999) 3.19

The physics of filopodial protrusion. Biophys J (2005) 3.00

Direct real-time observation of actin filament branching mediated by Arp2/3 complex using total internal reflection fluorescence microscopy. Proc Natl Acad Sci U S A (2001) 2.89

Motility of ActA protein-coated microspheres driven by actin polymerization. Proc Natl Acad Sci U S A (1999) 2.88

The dynamics of actin-based motility depend on surface parameters. Nature (2002) 2.78

Actin dynamics in vivo. Curr Opin Cell Biol (1997) 2.58

The Rho family GTPase Rif induces filopodia through mDia2. Curr Biol (2005) 2.58

Fascins, and their roles in cell structure and function. Bioessays (2002) 2.53

Asters, vortices, and rotating spirals in active gels of polar filaments. Phys Rev Lett (2004) 2.53

The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia. Nat Cell Biol (2005) 2.47

Formation of filopodia-like bundles in vitro from a dendritic network. J Cell Biol (2003) 2.44

Compression forces generated by actin comet tails on lipid vesicles. Proc Natl Acad Sci U S A (2003) 2.44

Control of actin assembly and disassembly at filament ends. Curr Opin Cell Biol (2000) 2.44

Identification of an actin binding region and a protein kinase C phosphorylation site on human fascin. J Biol Chem (1997) 2.02

Fascin-mediated propulsion of Listeria monocytogenes independent of frequent nucleation by the Arp2/3 complex. J Cell Biol (2004) 1.62

Polarity sorting of actin filaments in cytochalasin-treated fibroblasts. J Cell Sci (1997) 1.43

End versus side branching by Arp2/3 complex. Biophys J (2004) 1.33

Computational model of dynein-dependent self-organization of microtubule asters. J Cell Sci (2004) 0.99

Cell biology: barbed ends rule. Nature (2004) 0.91

Articles by these authors

Counting cytokinesis proteins globally and locally in fission yeast. Science (2005) 5.20

Mechanism of actin filament turnover by severing and nucleation at different concentrations of ADF/cofilin. Mol Cell (2006) 4.88

Control of the assembly of ATP- and ADP-actin by formins and profilin. Cell (2006) 4.37

Real-time measurements of actin filament polymerization by total internal reflection fluorescence microscopy. Biophys J (2004) 4.32

Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces. Proc Natl Acad Sci U S A (2004) 3.89

Assembly mechanism of the contractile ring for cytokinesis by fission yeast. Science (2007) 3.82

The fission yeast cytokinesis formin Cdc12p is a barbed end actin filament capping protein gated by profilin. J Cell Biol (2003) 3.40

Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis. Dev Cell (2003) 3.39

Forces and pressures in DNA packaging and release from viral capsids. Biophys J (2003) 3.04

The structural basis of actin filament branching by the Arp2/3 complex. J Cell Biol (2008) 2.82

A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complex. Nat Struct Biol (2003) 2.77

Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast. J Cell Biol (2006) 2.67

Polymerization kinetics of ADP- and ADP-Pi-actin determined by fluorescence microscopy. Proc Natl Acad Sci U S A (2007) 2.49

Hydrolysis of ATP by polymerized actin depends on the bound divalent cation but not profilin. Biochemistry (2002) 2.34

The extracellular architecture of adherens junctions revealed by crystal structures of type I cadherins. Structure (2011) 2.19

Myosin Va maneuvers through actin intersections and diffuses along microtubules. Proc Natl Acad Sci U S A (2007) 2.14

Review of the mechanism of processive actin filament elongation by formins. Cell Motil Cytoskeleton (2009) 2.12

Leiomodin is an actin filament nucleator in muscle cells. Science (2008) 2.05

The role of the FH1 domain and profilin in formin-mediated actin-filament elongation and nucleation. Curr Biol (2007) 2.01

Specificity of cell-cell adhesion by classical cadherins: Critical role for low-affinity dimerization through beta-strand swapping. Proc Natl Acad Sci U S A (2005) 1.99

UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II. J Cell Biol (2004) 1.98

Cofilin dissociates Arp2/3 complex and branches from actin filaments. Curr Biol (2009) 1.98

Model of formin-associated actin filament elongation. Mol Cell (2006) 1.92

Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast. J Cell Biol (2005) 1.84

Quantitative analysis of the mechanism of endocytic actin patch assembly and disassembly in fission yeast. Mol Biol Cell (2010) 1.79

Pathway of actin filament branch formation by Arp2/3 complex. J Biol Chem (2007) 1.78

Mathematical modeling of endocytic actin patch kinetics in fission yeast: disassembly requires release of actin filament fragments. Mol Biol Cell (2010) 1.76

A cytoskeletal demolition worker: myosin II acts as an actin depolymerization agent. J Mol Biol (2007) 1.74

Transforming binding affinities from three dimensions to two with application to cadherin clustering. Nature (2011) 1.73

Kinetics of the formation and dissociation of actin filament branches mediated by Arp2/3 complex. Biophys J (2006) 1.71

Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP. Proc Natl Acad Sci U S A (2004) 1.70

Insights into the influence of nucleotides on actin family proteins from seven structures of Arp2/3 complex. Mol Cell (2007) 1.70

Predicting the sizes of large RNA molecules. Proc Natl Acad Sci U S A (2008) 1.63

Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast. Mol Biol Cell (2005) 1.59

Structural and biochemical characterization of two binding sites for nucleation-promoting factor WASp-VCA on Arp2/3 complex. Proc Natl Acad Sci U S A (2011) 1.56

Xenopus actin-interacting protein 1 (XAip1) enhances cofilin fragmentation of filaments by capping filament ends. J Biol Chem (2002) 1.50

EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J Cell Biol (2003) 1.48

Cooperativity between trans and cis interactions in cadherin-mediated junction formation. Proc Natl Acad Sci U S A (2010) 1.46

Identification of functionally important residues of Arp2/3 complex by analysis of homology models from diverse species. J Mol Biol (2004) 1.45

Matrix formalism for site-specific binding of unstructured proteins to multicomponent lipid membranes. J Pept Sci (2008) 1.44

Arp2/3 complex-dependent actin networks constrain myosin II function in driving retrograde actin flow. J Cell Biol (2012) 1.42

Cytokinesis depends on the motor domains of myosin-II in fission yeast but not in budding yeast. Mol Biol Cell (2005) 1.41

Single molecule kinetic analysis of actin filament capping. Polyphosphoinositides do not dissociate capping proteins. J Biol Chem (2007) 1.37

Nucleotide-dependent conformational states of actin. Proc Natl Acad Sci U S A (2009) 1.33

Three-dimensional reconstructions of Arp2/3 complex with bound nucleation promoting factors. EMBO J (2011) 1.29

A malaria parasite formin regulates actin polymerization and localizes to the parasite-erythrocyte moving junction during invasion. Cell Host Microbe (2008) 1.27

Twinfilin is an actin-filament-severing protein and promotes rapid turnover of actin structures in vivo. J Cell Sci (2006) 1.20

Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring. J Cell Biol (2011) 1.17

Chapter 9: Counting proteins in living cells by quantitative fluorescence microscopy with internal standards. Methods Cell Biol (2008) 1.17

Visualizing Arp2/3 complex activation mediated by binding of ATP and WASp using structural mass spectrometry. Proc Natl Acad Sci U S A (2007) 1.16

Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit. J Biol Chem (2008) 1.15

Osmotic shock and the strength of viral capsids. Biophys J (2003) 1.15

Intracellular localization and dynamics of myosin-II and myosin-IC in live Acanthamoeba by transient transfection of EGFP fusion proteins. J Cell Sci (2002) 1.13

Distinct roles for F-BAR proteins Cdc15p and Bzz1p in actin polymerization at sites of endocytosis in fission yeast. Curr Biol (2011) 1.12

Domain formation induced by the adsorption of charged proteins on mixed lipid membranes. Biophys J (2004) 1.12

Energetic requirements for processive elongation of actin filaments by FH1FH2-formins. J Biol Chem (2009) 1.08

Nucleotide-mediated conformational changes of monomeric actin and Arp3 studied by molecular dynamics simulations. J Mol Biol (2007) 1.07

A statistical-thermodynamic model of viral budding. Biophys J (2004) 1.06

Tension modulates actin filament polymerization mediated by formin and profilin. Proc Natl Acad Sci U S A (2013) 1.04

Yeast UCS proteins promote actomyosin interactions and limit myosin turnover in cells. Proc Natl Acad Sci U S A (2008) 1.04

Formins filter modified actin subunits during processive elongation. J Struct Biol (2011) 1.03

Perturbation of a lipid membrane by amphipathic peptides and its role in pore formation. Eur Biophys J (2004) 1.03

Modulation of the spontaneous curvature and bending rigidity of lipid membranes by interfacially adsorbed amphipathic peptides. J Phys Chem B (2008) 1.02

Dynamic localization of myosin-I to endocytic structures in Acanthamoeba. Cell Motil Cytoskeleton (2003) 1.02

Determinants of Formin Homology 1 (FH1) domain function in actin filament elongation by formins. J Biol Chem (2012) 1.02

Structure and dynamics of the actin filament. J Mol Biol (2009) 1.01

Arp2/3 branched actin network mediates filopodia-like bundles formation in vitro. PLoS One (2008) 1.00

Progressing actin: Formin as a processive elongation machine. Nat Cell Biol (2004) 1.00

Membrane perturbation induced by interfacially adsorbed peptides. Biophys J (2004) 0.98

Thickness distribution of actin bundles in vitro. Eur Biophys J (2007) 0.98

Theory and simulations of adhesion receptor dimerization on membrane surfaces. Biophys J (2013) 0.97

Incompatibility with formin Cdc12p prevents human profilin from substituting for fission yeast profilin: insights from crystal structures of fission yeast profilin. J Biol Chem (2008) 0.96

Actin filament severing by cofilin dismantles actin patches and produces mother filaments for new patches. Curr Biol (2013) 0.96

Energetics and self-assembly of amphipathic peptide pores in lipid membranes. Biophys J (2003) 0.96

Macroion-induced compositional instability of binary fluid membranes. Phys Rev Lett (2002) 0.96

Cortactin releases the brakes in actin- based motility by enhancing WASP-VCA detachment from Arp2/3 branches. Curr Biol (2011) 0.95

Anillin-related protein Mid1p coordinates the assembly of the cytokinetic contractile ring in fission yeast. Mol Biol Cell (2012) 0.95

Hierarchical self-organization of cytoskeletal active networks. Phys Biol (2012) 0.91

Cooperation between the septins and the actomyosin ring and role of a cell-integrity pathway during cell division in fission yeast. Genetics (2010) 0.91

Cytokinetic nodes in fission yeast arise from two distinct types of nodes that merge during interphase. J Cell Biol (2014) 0.90

The size of RNA as an ideal branched polymer. J Chem Phys (2011) 0.90

High affinity, paralog-specific recognition of the Mena EVH1 domain by a miniature protein. J Am Chem Soc (2004) 0.89

Flexible charged macromolecules on mixed fluid lipid membranes: theory and Monte Carlo simulations. Biophys J (2005) 0.88

Purification of actin from fission yeast Schizosaccharomyces pombe and characterization of functional differences from muscle actin. J Biol Chem (2010) 0.87

Regulation of actin polymerization and adhesion-dependent cell edge protrusion by the Abl-related gene (Arg) tyrosine kinase and N-WASp. Biochemistry (2010) 0.86

From branched networks of actin filaments to bundles. Chemphyschem (2009) 0.85

Key structural features of the actin filament Arp2/3 complex branch junction revealed by molecular simulation. J Mol Biol (2011) 0.85

Measuring affinities of fission yeast spindle pole body proteins in live cells across the cell cycle. Biophys J (2013) 0.85

Characterization of structural and functional domains of the anillin-related protein Mid1p that contribute to cytokinesis in fission yeast. Mol Biol Cell (2012) 0.85

Molecular dynamics simulations of Arp2/3 complex activation. Biophys J (2010) 0.85

Nucleotide- and activator-dependent structural and dynamic changes of arp2/3 complex monitored by hydrogen/deuterium exchange and mass spectrometry. J Mol Biol (2009) 0.85

The "electrostatic-switch" mechanism: Monte Carlo study of MARCKS-membrane interaction. Biophys J (2008) 0.84