Quantitative real-time Legionella PCR for environmental water samples: data interpretation.

PubWeight™: 1.44‹?› | Rank: Top 5%

🔗 View Article (PMC 1449029)

Published in Appl Environ Microbiol on April 01, 2006

Authors

Philippe Joly1, Pierre-Alain Falconnet, Janine André, Nicole Weill, Monique Reyrolle, François Vandenesch, Max Maurin, Jerome Etienne, Sophie Jarraud

Author Affiliations

1: Centre National de Référence des Legionella, INSERM E-0230, Faculté de Médecine, IFR 62, 7 rue Guillaume-Paradin, 69372 Lyon Cedex 08, France.

Articles citing this

Molecular pathogenesis of infections caused by Legionella pneumophila. Clin Microbiol Rev (2010) 2.04

A PCR-based method for monitoring Legionella pneumophila in water samples detects viable but noncultivable legionellae that can recover their cultivability. Appl Environ Microbiol (2008) 1.22

Specific real-time PCR for simultaneous detection and identification of Legionella pneumophila serogroup 1 in water and clinical samples. Appl Environ Microbiol (2010) 1.17

Integrated real-time PCR for detection and monitoring of Legionella pneumophila in water systems. Appl Environ Microbiol (2006) 1.10

Viability PCR, a culture-independent method for rapid and selective quantification of viable Legionella pneumophila cells in environmental water samples. Appl Environ Microbiol (2009) 1.09

Evaluation of a nested-PCR-derived sequence-based typing method applied directly to respiratory samples from patients with Legionnaires' disease. J Clin Microbiol (2009) 1.08

An international trial of quantitative PCR for monitoring Legionella in artificial water systems. J Appl Microbiol (2011) 0.94

Caution on interpretation of legionella results obtained using real-time PCR for environmental water samples. Appl Environ Microbiol (2006) 0.88

Detection of legionella contamination in tabriz hospitals by PCR assay. Adv Pharm Bull (2013) 0.86

Limitations of Using Propidium Monoazide with qPCR to Discriminate between Live and Dead Legionella in Biofilm Samples. Microbiol Insights (2014) 0.85

Detection of Legionella by quantitative-polymerase chain reaction (qPCR) for monitoring and risk assessment. BMC Microbiol (2011) 0.84

Quantification of burkholderia coxL genes in Hawaiian volcanic deposits. Appl Environ Microbiol (2010) 0.80

Identification of legionella species by use of an oligonucleotide array. J Clin Microbiol (2009) 0.79

Investigation of Legionella Contamination in Bath Water Samples by Culture, Amoebic Co-Culture, and Real-Time Quantitative PCR Methods. Int J Environ Res Public Health (2015) 0.78

Control of Legionella Contamination and Risk of Corrosion in Hospital Water Networks following Various Disinfection Procedures. Appl Environ Microbiol (2016) 0.78

Detection and identification of Legionella species in hospital water supplies through Polymerase Chain Reaction (16S rRNA). J Environ Health Sci Eng (2014) 0.77

Detection and quantification of Flavobacterium psychrophilum in water and fish tissue samples by quantitative real time PCR. BMC Microbiol (2014) 0.76

Dynamics of Bacillus thuringiensis var. israelensis and Lysinibacillus sphaericus spores in urban catch basins after simultaneous application against mosquito larvae. PLoS One (2013) 0.76

The Efficacy of Residual Chlorine Content on the Control of Legionella Spp. In Hospital Water Systems. Iran J Public Health (2014) 0.75

Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems. BMC Microbiol (2017) 0.75

Detection and Quantification of Legionella pneumophila from Water Systems in Kuwait Residential Facilities. J Pathog (2012) 0.75

On-filter direct amplification of Legionella pneumophila for rapid assessment of its abundance and viability. Water Res (2017) 0.75

Articles cited by this

Legionella and Legionnaires' disease: 25 years of investigation. Clin Microbiol Rev (2002) 7.42

The genomic sequence of the accidental pathogen Legionella pneumophila. Science (2004) 4.08

Distribution of Legionella species and serogroups isolated by culture in patients with sporadic community-acquired legionellosis: an international collaborative survey. J Infect Dis (2002) 3.65

Water-related nosocomial pneumonia caused by Legionella pneumophila serogroups 1 and 10. J Infect Dis (1985) 3.16

Enzyme-linked immunoassay for detection of PCR-amplified DNA of legionellae in bronchoalveolar fluid. J Clin Microbiol (1995) 3.04

Detection of Legionella species in respiratory specimens using PCR with sequencing confirmation. J Clin Microbiol (2000) 2.90

Legionellaceae in the hospital water-supply. Epidemiological link with disease and evaluation of a method for control of nosocomial legionnaires' disease and Pittsburgh pneumonia. Lancet (1983) 2.60

Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii. Appl Environ Microbiol (1997) 2.45

Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR. Appl Environ Microbiol (2001) 2.43

Hospital characteristics associated with colonization of water systems by Legionella and risk of nosocomial legionnaires' disease: a cohort study of 15 hospitals. Infect Control Hosp Epidemiol (1999) 2.20

Clinical and environmental distributions of Legionella strains in France are different. J Clin Microbiol (2004) 1.91

Development of a new seminested PCR method for detection of Legionella species and its application to surveillance of legionellae in hospital cooling tower water. Appl Environ Microbiol (1997) 1.88

Comparison of detection methods for Legionella species in environmental water by colony isolation, fluorescent antibody staining, and polymerase chain reaction. Microbiol Immunol (1993) 1.83

Detection of Legionella pneumophila using a real-time PCR hybridization assay. J Clin Microbiol (2000) 1.69

Rapid method for enumeration of viable Legionella pneumophila and other Legionella spp. in water. Appl Environ Microbiol (2005) 1.35

Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method. Appl Environ Microbiol (1993) 1.14

Quantitative detection of Legionella pneumophila in water samples by immunomagnetic purification and real-time PCR amplification of the dotA gene. Appl Environ Microbiol (2005) 1.10

Precision and accuracy of recovery of Legionella pneumophila from seeded tap water by filtration and centrifugation. Appl Environ Microbiol (1995) 1.03

Evaluation of detection of Legionella spp. in water samples by fluorescence in situ hybridization, PCR amplification and bacterial culture. Int J Med Microbiol (2002) 1.02

Comparison of polymerase chain reaction and conventional culture for the detection of legionellae in cooling tower waters in Singapore. Lett Appl Microbiol (1997) 1.02

Guidelines for investigating single cases of Legionnaires' disease. Commun Dis Public Health (2002) 0.97

Routine sampling and the control of Legionella spp. in cooling tower water systems. Curr Microbiol (2000) 0.95

Fatal nosocomial Legionnaires' disease: relevance of contamination of hospital water supply by temperature-dependent buoyancy-driven flow from spur pipes. Epidemiol Infect (1994) 0.93

Enumeration of Legionella pneumophila in cooling tower water systems. Environ Monit Assess (2005) 0.85

Articles by these authors

Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: worldwide emergence. Emerg Infect Dis (2003) 13.83

Comparison of community- and health care-associated methicillin-resistant Staphylococcus aureus infection. JAMA (2003) 12.04

Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. Lancet (2002) 10.26

Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infect Immun (2002) 7.34

Staphylococcus aureus Panton-Valentine leukocidin causes necrotizing pneumonia. Science (2007) 7.06

Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Chemother (2006) 7.03

Community-acquired methicillin-resistant Staphylococcus aureus infections in France: emergence of a single clone that produces Panton-Valentine leukocidin. Clin Infect Dis (2002) 5.75

Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity. Nat Genet (2004) 4.93

Changing profile of infective endocarditis: results of a 1-year survey in France. JAMA (2002) 4.48

Global distribution of Panton-Valentine leukocidin--positive methicillin-resistant Staphylococcus aureus, 2006. Emerg Infect Dis (2007) 3.98

Comprehensive diagnostic strategy for blood culture-negative endocarditis: a prospective study of 819 new cases. Clin Infect Dis (2010) 3.84

Bacterial competition for human nasal cavity colonization: role of Staphylococcal agr alleles. Appl Environ Microbiol (2003) 3.51

Factors predicting mortality in necrotizing community-acquired pneumonia caused by Staphylococcus aureus containing Panton-Valentine leukocidin. Clin Infect Dis (2007) 3.31

Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila. J Clin Microbiol (2005) 3.21

Polymorphonuclear leukocytes mediate Staphylococcus aureus Panton-Valentine leukocidin-induced lung inflammation and injury. Proc Natl Acad Sci U S A (2010) 3.05

Staphylococcus aureus RNAIII and the endoribonuclease III coordinately regulate spa gene expression. EMBO J (2005) 2.95

Staphylococcus aureus RNAIII coordinately represses the synthesis of virulence factors and the transcription regulator Rot by an antisense mechanism. Genes Dev (2007) 2.86

Staphylococcus aureus Panton-Valentine leukocidin directly targets mitochondria and induces Bax-independent apoptosis of human neutrophils. J Clin Invest (2005) 2.82

Isotope-labeled protein standards: toward absolute quantitative proteomics. Mol Cell Proteomics (2007) 2.63

A community-wide outbreak of legionnaires disease linked to industrial cooling towers--how far can contaminated aerosols spread? J Infect Dis (2005) 2.38

Relationship between baseline clinical data and microbiologic spectrum in 100 patients with acute postcataract endophthalmitis. Retina (2012) 2.12

The role of RNAs in the regulation of virulence-gene expression. Curr Opin Microbiol (2006) 2.09

Preeminence of Staphylococcus aureus in infective endocarditis: a 1-year population-based survey. Clin Infect Dis (2012) 1.97

Are host genetics the predominant determinant of persistent nasal Staphylococcus aureus carriage in humans? J Infect Dis (2010) 1.92

Clinical and environmental distributions of Legionella strains in France are different. J Clin Microbiol (2004) 1.91

Molecular diagnosis of infective endocarditis by PCR amplification and direct sequencing of DNA from valve tissue. J Clin Microbiol (2003) 1.90

Specific real-time polymerase chain reaction places Kingella kingae as the most common cause of osteoarticular infections in young children. Pediatr Infect Dis J (2007) 1.89

Multigenome analysis identifies a worldwide distributed epidemic Legionella pneumophila clone that emerged within a highly diverse species. Genome Res (2008) 1.84

Effect of antibiotics on Staphylococcus aureus producing Panton-Valentine leukocidin. Antimicrob Agents Chemother (2007) 1.84

A search for small noncoding RNAs in Staphylococcus aureus reveals a conserved sequence motif for regulation. Nucleic Acids Res (2009) 1.81

High genetic variability of the agr locus in Staphylococcus species. J Bacteriol (2002) 1.78

Neutralization of Staphylococcus aureus Panton Valentine leukocidin by intravenous immunoglobulin in vitro. J Infect Dis (2004) 1.76

Outcome and treatment of Bartonella endocarditis. Arch Intern Med (2003) 1.74

The staphylococcal toxin Panton-Valentine Leukocidin targets human C5a receptors. Cell Host Microbe (2013) 1.66

Pediatric bone and joint infections caused by Panton-Valentine leukocidin-positive Staphylococcus aureus. Pediatr Infect Dis J (2007) 1.64

A multicenter evaluation of genotypic methods for the epidemiologic typing of Legionella pneumophila serogroup 1: results of a pan-European study. Clin Microbiol Infect (1999) 1.61

Use of multiplex PCR to identify Staphylococcus aureus adhesins involved in human hematogenous infections. J Clin Microbiol (2003) 1.61

Virulence determinants in community and hospital meticillin-resistant Staphylococcus aureus. J Hosp Infect (2007) 1.57

Global distribution and evolution of Panton-Valentine leukocidin-positive methicillin-susceptible Staphylococcus aureus, 1981-2007. J Infect Dis (2010) 1.54

Comparative prevalence of superantigen genes in Staphylococcus aureus isolates causing sepsis with and without septic shock. Clin Infect Dis (2005) 1.53

Evaluation of antibiotic susceptibilities of three rickettsial species including Rickettsia felis by a quantitative PCR DNA assay. Antimicrob Agents Chemother (2002) 1.53

Community-acquired methicillin-resistant Staphylococcus aureus isolated in Switzerland contains the Panton-Valentine leukocidin or exfoliative toxin genes. J Clin Microbiol (2004) 1.48

A history of Panton-Valentine leukocidin (PVL)-associated infection protects against death in PVL-associated pneumonia. Vaccine (2011) 1.48

Panton-valentine leukocidin enhances the severity of community-associated methicillin-resistant Staphylococcus aureus rabbit osteomyelitis. PLoS One (2009) 1.48

Analysis of the Legionella longbeachae genome and transcriptome uncovers unique strategies to cause Legionnaires' disease. PLoS Genet (2010) 1.46

Detection of new methicillin-resistant Staphylococcus aureus clones containing the toxic shock syndrome toxin 1 gene responsible for hospital- and community-acquired infections in France. J Clin Microbiol (2006) 1.46

Contribution of a broad range polymerase chain reaction to the diagnosis of osteoarticular infections caused by Kingella kingae: description of twenty-four recent pediatric diagnoses. Pediatr Infect Dis J (2005) 1.45

Ocular manifestations of syphilis: recent cases over a 2.5-year period. Graefes Arch Clin Exp Ophthalmol (2010) 1.44

Panton-valentine leukocidin and staphyloccoccal skin infections in schoolchildren. Emerg Infect Dis (2004) 1.41

Can Whipple's disease be transmitted by gastroscopes? Infect Control Hosp Epidemiol (2003) 1.40

Identification of the capsular polysaccharides in Staphylococcus aureus clinical isolates by PCR and agglutination tests. J Clin Microbiol (2007) 1.40

The Staphylococcus aureus RNome and its commitment to virulence. PLoS Pathog (2011) 1.39

MRSA harboring mecA variant gene mecC, France. Emerg Infect Dis (2012) 1.39

Methicillin-resistant Staphylococcus capitis with reduced vancomycin susceptibility causes late-onset sepsis in intensive care neonates. PLoS One (2012) 1.37

Inactivation of mprF affects vancomycin susceptibility in Staphylococcus aureus. Biochim Biophys Acta (2003) 1.35

Epidemiology of invasive methicillin-resistant Staphylococcus aureus clones collected in France in 2006 and 2007. J Clin Microbiol (2008) 1.35

Methicillin-resistant Staphylococcus aureus in neonatal intensive care unit. Emerg Infect Dis (2005) 1.32

Extensive recombination events and horizontal gene transfer shaped the Legionella pneumophila genomes. BMC Genomics (2011) 1.32

Pneumonia and new methicillin-resistant Staphylococcus aureus clone. Emerg Infect Dis (2006) 1.28

Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation. PLoS Pathog (2010) 1.27

Clonal distribution and differential occurrence of the enterotoxin gene cluster, egc, in carriage- versus bacteremia-associated isolates of Staphylococcus aureus. J Clin Microbiol (2006) 1.27

Human infection with Schineria iarvae. Emerg Infect Dis (2007) 1.26

Lethal necrotizing pneumonia caused by an ST398 Staphylococcus aureus strain. Emerg Infect Dis (2010) 1.26

A multicenter evaluation of the Biotest legionella urinary antigen EIA. Clin Microbiol Infect (1998) 1.26

PSMs of hypervirulent Staphylococcus aureus act as intracellular toxins that kill infected osteoblasts. PLoS One (2013) 1.26

Panton-Valentine leukocidin: a marker of severity for Staphylococcus aureus infection? Clin Infect Dis (2005) 1.25

Clinical and environmental isolates of Legionella pneumophila serogroup 1 cannot be distinguished by sequence analysis of two surface protein genes and three housekeeping genes. Appl Environ Microbiol (2005) 1.25

Staphylococcal enterotoxin-like toxins U2 and V, two new staphylococcal superantigens arising from recombination within the enterotoxin gene cluster. Infect Immun (2006) 1.24