Transposon mutagenesis identifies sites upstream of the Neisseria gonorrhoeae pilE gene that modulate pilin antigenic variation.

PubWeight™: 1.04‹?› | Rank: Top 15%

🔗 View Article (PMC 1855897)

Published in J Bacteriol on February 16, 2007

Authors

Kimberly A Kline1, Alison K Criss, Anne Wallace, H Steven Seifert

Author Affiliations

1: Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 303 East Chicago Ave., Chicago, IL 60620, USA.

Articles citing this

An alternative DNA structure is necessary for pilin antigenic variation in Neisseria gonorrhoeae. Science (2009) 2.37

Transcription of a cis-acting, noncoding, small RNA is required for pilin antigenic variation in Neisseria gonorrhoeae. PLoS Pathog (2013) 1.72

Microbial antigenic variation mediated by homologous DNA recombination. FEMS Microbiol Rev (2012) 1.18

Focusing homologous recombination: pilin antigenic variation in the pathogenic Neisseria. Mol Microbiol (2011) 1.01

Pilin antigenic variation occurs independently of the RecBCD pathway in Neisseria gonorrhoeae. J Bacteriol (2009) 0.96

Frequency and rate of pilin antigenic variation of Neisseria meningitidis. J Bacteriol (2010) 0.96

Inhibition of IL-10 production by maternal antibodies against Group B Streptococcus GAPDH confers immunity to offspring by favoring neutrophil recruitment. PLoS Pathog (2011) 0.89

Role for the RecBCD recombination pathway for pilE gene variation in repair-proficient Neisseria gonorrhoeae. J Bacteriol (2007) 0.88

Neisseria gonorrhoeae RecQ helicase HRDC domains are essential for efficient binding and unwinding of the pilE guanine quartet structure required for pilin antigenic variation. J Bacteriol (2013) 0.85

Identification of bacterial protein O-oligosaccharyltransferases and their glycoprotein substrates. PLoS One (2013) 0.82

Antigenic Variation in Bacterial Pathogens. Microbiol Spectr (2016) 0.78

Neisseria gonorrhoeae MutS affects pilin antigenic variation through mismatch correction and not by pilE guanine quartet binding. J Bacteriol (2015) 0.77

Articles cited by this

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A (2000) 96.90

NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol (1963) 45.03

Elevated recombination rates in transcriptionally active DNA. Cell (1989) 17.42

Pilus expression in Neisseria gonorrhoeae involves chromosomal rearrangement. Cell (1982) 7.19

Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus. Cell (1982) 7.00

Pilus genes of Neisseria gonorrheae: chromosomal organization and DNA sequence. Proc Natl Acad Sci U S A (1984) 5.37

A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae. Cell (1983) 5.05

Phase and antigenic variation in bacteria. Clin Microbiol Rev (2004) 5.02

The repertoire of silent pilus genes in Neisseria gonorrhoeae: evidence for gene conversion. Cell (1986) 4.48

Mating-type gene switching in Saccharomyces cerevisiae. Annu Rev Genet (1998) 4.08

Identification and arrangement of the DNA sequence recognized in specific transformation of Neisseria gonorrhoeae. Proc Natl Acad Sci U S A (1988) 4.04

Class-switch recombination: interplay of transcription, DNA deamination and DNA repair. Nat Rev Immunol (2004) 4.04

Tn5 in vitro transposition. J Biol Chem (1998) 3.66

A 24-base-pair DNA sequence from the MAT locus stimulates intergenic recombination in yeast. Proc Natl Acad Sci U S A (1986) 3.40

Role of chromosomal rearrangement in N. gonorrhoeae pilus phase variation. Cell (1985) 3.30

The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins. Cell (1997) 3.09

IHF and HU: flexible architects of bent DNA. Curr Opin Struct Biol (2004) 2.80

Antigenic variation of gonococcal pilus involves assembly of separated silent gene segments. Proc Natl Acad Sci U S A (1986) 2.53

Effects of recA mutations on pilus antigenic variation and phase transitions in Neisseria gonorrhoeae. Genetics (1987) 2.39

Differential roles of homologous recombination pathways in Neisseria gonorrhoeae pilin antigenic variation, DNA transformation and DNA repair. Mol Microbiol (1998) 2.24

An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY. EMBO J (1994) 2.13

FtsY, the prokaryotic signal recognition particle receptor homologue, is essential for biogenesis of membrane proteins. J Biol Chem (1997) 1.94

Multiple gonococcal pilin antigenic variants are produced during experimental human infections. J Clin Invest (1994) 1.93

Insertionally inactivated and inducible recA alleles for use in Neisseria. Gene (1997) 1.85

A homologue of the recombination-dependent growth gene, rdgC, is involved in gonococcal pilin antigenic variation. Genetics (2000) 1.83

Molecular models accounting for the gene conversion reactions mediating gonococcal pilin antigenic variation. Mol Microbiol (2000) 1.70

Mutagenesis of Neisseria meningitidis by in vitro transposition of Himar1 mariner. J Bacteriol (2000) 1.65

The frequency and rate of pilin antigenic variation in Neisseria gonorrhoeae. Mol Microbiol (2005) 1.65

Mechanisms for gene conversion and homologous recombination: the double-strand break repair model and the successive half crossing-over model. Adv Biophys (1992) 1.64

Roles of the recJ and recN genes in homologous recombination and DNA repair pathways of Neisseria gonorrhoeae. J Bacteriol (2002) 1.64

The recX gene potentiates homologous recombination in Neisseria gonorrhoeae. Mol Microbiol (2001) 1.54

A V(D)J recombinase-inducible B-cell line: role of transcriptional enhancer elements in directing V(D)J recombination. Mol Cell Biol (1993) 1.54

Transcription promotes recA-independent recombination mediated by DNA-dependent RNA polymerase in Escherichia coli. Proc Natl Acad Sci U S A (1979) 1.54

Efficacy trial of a parenteral gonococcal pilus vaccine in men. Vaccine (1991) 1.50

A genetic screen identifies genes and sites involved in pilin antigenic variation in Neisseria gonorrhoeae. Mol Microbiol (2005) 1.47

A conserved DNA sequence is required for efficient gonococcal pilin antigenic variation. Mol Microbiol (1994) 1.44

Control of pilus expression in Neisseria gonorrhoeae as an original system in the family of two-component regulators. Mol Microbiol (1991) 1.24

Restricted VH gene usage and generation of antibody diversity in rabbit. Annu Rev Immunol (1992) 1.21

Neisseria gonorrhoeae DNA recombination and repair enzymes protect against oxidative damage caused by hydrogen peroxide. J Bacteriol (2006) 1.21

Antigenic variation of gonococcal pilin expression in vivo: analysis of the strain FA1090 pilin repertoire and identification of the pilS gene copies recombining with pilE during experimental human infection. Microbiology (2001) 1.19

The role of direct oligonucleotide repeats in gonococcal pilin gene variation. Mol Microbiol (1990) 1.19

The size and position of heterologous insertions in a silent locus differentially affect pilin recombination in Neisseria gonorrhoeae. Mol Microbiol (1996) 1.15

Loss of both Holliday junction processing pathways is synthetically lethal in the presence of gonococcal pilin antigenic variation. Mol Microbiol (2006) 1.15

The pilE gene of Neisseria gonorrhoeae MS11 is transcribed from a sigma 70 promoter during growth in vitro. J Bacteriol (1995) 1.15

Role of the Rep helicase gene in homologous recombination in Neisseria gonorrhoeae. J Bacteriol (2005) 1.13

Random shuttle mutagenesis: gonococcal mutants deficient in pilin antigenic variation. Mol Microbiol (1997) 1.13

Mutation of the priA gene of Neisseria gonorrhoeae affects DNA transformation and DNA repair. J Bacteriol (2005) 1.12

Iron availability regulates DNA recombination in Neisseria gonorrhoeae. Mol Microbiol (2000) 1.11

Analysis of protein binding to the Sma/Cla DNA repeat in pathogenic Neisseriae. Nucleic Acids Res (1997) 1.00

Integration host factor is a transcriptional cofactor of pilE in Neisseria gonorrhoeae. Mol Microbiol (1997) 1.00

The RpoH-mediated stress response in Neisseria gonorrhoeae is regulated at the level of activity. J Bacteriol (2004) 0.98

An AT-rich tract containing an integration host factor-binding domain and two UP-like elements enhances transcription from the pilEp1 promoter of Neisseria gonorrhoeae. J Bacteriol (1998) 0.92

DNA sequence bias during Tn5 transposition. J Mol Biol (2004) 0.89

Insertion mutations in pilE differentially alter gonococcal pilin antigenic variation. J Bacteriol (1999) 0.88

Many levels of control of V gene rearrangement frequency. Immunol Rev (2004) 0.88

Neisseria gonorrhoeae PilA is an FtsY homolog. J Bacteriol (1999) 0.86

Tn5 insertion specificity is not influenced by IS50 end sequences in target DNA. Mol Gen Genet (1991) 0.84

Interaction of the Neisseria gonorrhoeae PilA protein with the pilE promoter involves multiple sites on the DNA. J Bacteriol (1995) 0.84

Control of gonococcal pilin-encoding gene expression in Escherichia coli. Gene (1993) 0.84

Immunoglobulin gene diversification by gene conversion. Prog Nucleic Acid Res Mol Biol (1993) 0.84

Transcription-mediated hyper-recombination in HOT1. Genes Cells (2004) 0.84

Identification of a protein that binds to the Ho endonuclease recognition sequence at the yeast mating type locus. Mol Cell Biol (1997) 0.80

Role for both DNA and RNA in GTP hydrolysis by the Neisseria gonorrhoeae signal recognition particle receptor. J Bacteriol (2003) 0.78

Articles by these authors

Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis. Mol Cell (2013) 2.52

An alternative DNA structure is necessary for pilin antigenic variation in Neisseria gonorrhoeae. Science (2009) 2.37

Phase I trial of adoptive immunotherapy with cytolytic T lymphocytes immunized against a tyrosinase epitope. J Clin Oncol (2002) 2.33

The obligate human pathogen, Neisseria gonorrhoeae, is polyploid. PLoS Biol (2006) 1.84

Transcription of a cis-acting, noncoding, small RNA is required for pilin antigenic variation in Neisseria gonorrhoeae. PLoS Pathog (2013) 1.72

The frequency and rate of pilin antigenic variation in Neisseria gonorrhoeae. Mol Microbiol (2005) 1.65

Roles of the recJ and recN genes in homologous recombination and DNA repair pathways of Neisseria gonorrhoeae. J Bacteriol (2002) 1.64

The transcriptome response of Neisseria gonorrhoeae to hydrogen peroxide reveals genes with previously uncharacterized roles in oxidative damage protection. Mol Microbiol (2005) 1.61

The outer membrane localization of the Neisseria gonorrhoeae MsrA/B is involved in survival against reactive oxygen species. Proc Natl Acad Sci U S A (2002) 1.55

Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo. J Biol Chem (2002) 1.49

Recombination, repair and replication in the pathogenic Neisseriae: the 3 R's of molecular genetics of two human-specific bacterial pathogens. Mol Microbiol (2003) 1.48

A genetic screen identifies genes and sites involved in pilin antigenic variation in Neisseria gonorrhoeae. Mol Microbiol (2005) 1.47

The penC mutation conferring antibiotic resistance in Neisseria gonorrhoeae arises from a mutation in the PilQ secretin that interferes with multimer stability. Mol Microbiol (2005) 1.41

Low-level pilin expression allows for substantial DNA transformation competence in Neisseria gonorrhoeae. Infect Immun (2003) 1.26

Resistance of Neisseria gonorrhoeae to non-oxidative killing by adherent human polymorphonuclear leucocytes. Cell Microbiol (2009) 1.24

Opportunity and means: horizontal gene transfer from the human host to a bacterial pathogen. MBio (2011) 1.23

Neisseria gonorrhoeae suppresses the oxidative burst of human polymorphonuclear leukocytes. Cell Microbiol (2008) 1.22

Neisseria gonorrhoeae DNA recombination and repair enzymes protect against oxidative damage caused by hydrogen peroxide. J Bacteriol (2006) 1.21

A mutant form of the Neisseria gonorrhoeae pilus secretin protein PilQ allows increased entry of heme and antimicrobial compounds. J Bacteriol (2004) 1.20

Microbial antigenic variation mediated by homologous DNA recombination. FEMS Microbiol Rev (2012) 1.18

The DinI and RecX proteins are competing modulators of RecA function. J Biol Chem (2004) 1.17

Loss of both Holliday junction processing pathways is synthetically lethal in the presence of gonococcal pilin antigenic variation. Mol Microbiol (2006) 1.15

A bacterial siren song: intimate interactions between Neisseria and neutrophils. Nat Rev Microbiol (2012) 1.14

DNA uptake sequence-mediated enhancement of transformation in Neisseria gonorrhoeae is strain dependent. J Bacteriol (2010) 1.14

Role of the Rep helicase gene in homologous recombination in Neisseria gonorrhoeae. J Bacteriol (2005) 1.13

Mutation of the priA gene of Neisseria gonorrhoeae affects DNA transformation and DNA repair. J Bacteriol (2005) 1.12

Breast Cancer Risk Reduction, Version 2.2015. J Natl Compr Canc Netw (2015) 1.12

Resistance of Neisseria gonorrhoeae to neutrophils. Front Microbiol (2011) 1.06

The RecF protein antagonizes RecX function via direct interaction. Mol Cell (2006) 1.05

A real-time semi-quantitative RT-PCR assay demonstrates that the pilE sequence dictates the frequency and characteristics of pilin antigenic variation in Neisseria gonorrhoeae. Nucleic Acids Res (2005) 1.02

IL-2- and STAT5-regulated cytokine gene expression in cells expressing the Tax protein of HTLV-1. Oncogene (2005) 1.01

Focusing homologous recombination: pilin antigenic variation in the pathogenic Neisseria. Mol Microbiol (2011) 1.01

Complexes of RecA with LexA and RecX differentiate between active and inactive RecA nucleoprotein filaments. J Mol Biol (2003) 0.98

Frequency and rate of pilin antigenic variation of Neisseria meningitidis. J Bacteriol (2010) 0.96

Pilin antigenic variation occurs independently of the RecBCD pathway in Neisseria gonorrhoeae. J Bacteriol (2009) 0.96

Neisseria gonorrhoeae phagosomes delay fusion with primary granules to enhance bacterial survival inside human neutrophils. Cell Microbiol (2013) 0.96

The efficacy of breast MRI in predicting breast conservation therapy. J Surg Oncol (2006) 0.95

RecA-binding pilE G4 sequence essential for pilin antigenic variation forms monomeric and 5' end-stacked dimeric parallel G-quadruplexes. Structure (2012) 0.94

Adaptive trials in the neoadjuvant setting: a model to safely tailor care while accelerating drug development. J Clin Oncol (2012) 0.93

Constitutively Opa-expressing and Opa-deficient neisseria gonorrhoeae strains differentially stimulate and survive exposure to human neutrophils. J Bacteriol (2013) 0.93

pilQ Missense mutations have diverse effects on PilQ multimer formation, piliation, and pilus function in Neisseria gonorrhoeae. J Bacteriol (2007) 0.93

Initial clinical experience with the Strut-Adjusted Volume Implant (SAVI) breast brachytherapy device for accelerated partial-breast irradiation (APBI): first 100 patients with more than 1 year of follow-up. Int J Radiat Oncol Biol Phys (2010) 0.92

Gonococci exit apically and basally from polarized epithelial cells and exhibit dynamic changes in type IV pili. Cell Microbiol (2006) 0.92

Genomic content of Neisseria species. J Bacteriol (2010) 0.92

Neisseria gonorrhoeae-mediated inhibition of apoptotic signalling in polymorphonuclear leukocytes. Infect Immun (2011) 0.91

Analysis of the Piv recombinase-related gene family of Neisseria gonorrhoeae. J Bacteriol (2005) 0.91

The genetics of Neisseria species. Annu Rev Genet (2014) 0.89

Differential cross-complementation patterns of Escherichia coli and Neisseria gonorrhoeae RecA proteins. Microbiology (2002) 0.87

Less is more: Neisseria gonorrhoeae RecX protein stimulates recombination by inhibiting RecA. J Biol Chem (2010) 0.87

Mismatch correction modulates mutation frequency and pilus phase and antigenic variation in Neisseria gonorrhoeae. J Bacteriol (2010) 0.87

Neisseria gonorrhoeae metalloprotease NGO1686 is required for full piliation, and piliation is required for resistance to H2O2- and neutrophil-mediated killing. MBio (2013) 0.86

Neisseria gonorrhoeae virulence factor NG1686 is a bifunctional M23B family metallopeptidase that influences resistance to hydrogen peroxide and colony morphology. J Biol Chem (2012) 0.85

Neisseria gonorrhoeae RecQ helicase HRDC domains are essential for efficient binding and unwinding of the pilE guanine quartet structure required for pilin antigenic variation. J Bacteriol (2013) 0.85

Assembly of NADPH oxidase in human neutrophils is modulated by the opacity-associated protein expression State of Neisseria gonorrhoeae. Infect Immun (2013) 0.84

Opa+ Neisseria gonorrhoeae exhibits reduced survival in human neutrophils via Src family kinase-mediated bacterial trafficking into mature phagolysosomes. Cell Microbiol (2014) 0.84

Initial clinical experience with the Strut-Adjusted Volume Implant brachytherapy applicator for accelerated partial breast irradiation. Brachytherapy (2009) 0.83

Purification and characterization of the RecA protein from Neisseria gonorrhoeae. PLoS One (2011) 0.82

Genetic characterization of the nucleotide excision repair system of Neisseria gonorrhoeae. J Bacteriol (2009) 0.82

The DNA-binding activity of the Neisseria gonorrhoeae LexA orthologue NG1427 is modulated by oxidation. Mol Microbiol (2010) 0.81

ksgA mutations confer resistance to kasugamycin in Neisseria gonorrhoeae. Int J Antimicrob Agents (2008) 0.81

Genetic transformation of Neisseria gonorrhoeae shows a strand preference. FEMS Microbiol Lett (2012) 0.80

Structure-function studies of the Neisseria gonorrhoeae major outer membrane porin. Infect Immun (2013) 0.80

Mobile DNA in the Pathogenic Neisseria. Microbiol Spectr (2015) 0.79

Interactions with Host Cells Causes Neisseria meningitidis Pili to Become Unglued. Front Microbiol (2011) 0.78

Fluorescence microscopy methods for determining the viability of bacteria in association with mammalian cells. J Vis Exp (2013) 0.78

Antigenic Variation in Bacterial Pathogens. Microbiol Spectr (2016) 0.78

The Neisseria gonorrhoeae photolyase orthologue phrB is required for proper DNA supercoiling but does not function in photo-reactivation. Mol Microbiol (2010) 0.78

Seminal plasma initiates a Neisseria gonorrhoeae transmission state. MBio (2014) 0.78

Neisseria gonorrhoeae and humans perform an evolutionary LINE dance. Mob Genet Elements (2011) 0.77

The misidentification of bacterial genes as human cDNAs: was the human D-1 tumor antigen gene acquired from bacteria? Genomics (2002) 0.77

Phase variation leads to the misidentification of a Neisseria gonorrhoeae virulence gene. PLoS One (2013) 0.76

Quantitative comparison of surgical margin histology following excision with traditional electrosurgery and a low-thermal-injury dissection device. J Surg Oncol (2011) 0.76

Saturating mutagenesis of an essential gene: a majority of the Neisseria gonorrhoeae major outer membrane porin (PorB) is mutable. J Bacteriol (2013) 0.76

Pathogenesis of Neisseria gonorrhoeae in the female reproductive tract: neutrophilic host response, sustained infection, and clinical sequelae. Curr Opin Hematol (2017) 0.75