Less is more: Neisseria gonorrhoeae RecX protein stimulates recombination by inhibiting RecA.

PubWeight™: 0.87‹?›

🔗 View Article (PMC 2988325)

Published in J Biol Chem on September 17, 2010

Authors

Marielle C Gruenig1, Elizabeth A Stohl, Sindhu Chitteni-Pattu, H Steven Seifert, Michael M Cox

Author Affiliations

1: Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.

Articles citing this

Microbial antigenic variation mediated by homologous DNA recombination. FEMS Microbiol Rev (2012) 1.18

Deep sequencing-based analysis of the anaerobic stimulon in Neisseria gonorrhoeae. BMC Genomics (2011) 1.14

Focusing homologous recombination: pilin antigenic variation in the pathogenic Neisseria. Mol Microbiol (2011) 1.01

The cell pole: the site of cross talk between the DNA uptake and genetic recombination machinery. Crit Rev Biochem Mol Biol (2012) 0.94

RecX facilitates homologous recombination by modulating RecA activities. PLoS Genet (2012) 0.82

Purification and characterization of the RecA protein from Neisseria gonorrhoeae. PLoS One (2011) 0.82

Mechanical force antagonizes the inhibitory effects of RecX on RecA filament formation in Mycobacterium tuberculosis. Nucleic Acids Res (2014) 0.80

Active displacement of RecA filaments by UvrD translocase activity. Nucleic Acids Res (2015) 0.80

Biochemical characterization of RecA variants that contribute to extreme resistance to ionizing radiation. DNA Repair (Amst) (2014) 0.78

Directed Evolution of RecA Variants with Enhanced Capacity for Conjugational Recombination. PLoS Genet (2015) 0.76

The RecX protein interacts with the RecA protein and modulates its activity in Herbaspirillum seropedicae. Braz J Med Biol Res (2012) 0.76

Mobile DNA in the pathogenic Neisseria. Microbiol Spectr (2015) 0.76

Analyzing Neisseria gonorrhoeae Pilin Antigenic Variation Using 454 Sequencing Technology. J Bacteriol (2016) 0.75

DNA Metabolism in Balance: Rapid Loss of a RecA-Based Hyperrec Phenotype. PLoS One (2016) 0.75

Bacillus subtilis RecA with DprA-SsbA antagonizes RecX function during natural transformation. Nucleic Acids Res (2017) 0.75

Articles cited by this

New M13 vectors for cloning. Methods Enzymol (1983) 90.74

NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol (1963) 45.03

The SOS regulatory system of Escherichia coli. Cell (1982) 16.90

Studies on gonococcus infection. I. Pili and zones of adhesion: their relation to gonococcal growth patterns. J Exp Med (1971) 14.84

The importance of repairing stalled replication forks. Nature (2000) 7.93

Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda. Microbiol Mol Biol Rev (1999) 6.92

Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures. Nature (2008) 5.85

Nature of the SOS-inducing signal in Escherichia coli. The involvement of DNA replication. J Mol Biol (1990) 4.84

Initiation of genetic recombination and recombination-dependent replication. Trends Biochem Sci (2000) 4.82

Intragenic recombination leads to pilus antigenic variation in Neisseria gonorrhoeae. Nature (1985) 4.60

The repertoire of silent pilus genes in Neisseria gonorrhoeae: evidence for gene conversion. Cell (1986) 4.48

Two binding modes in Escherichia coli single strand binding protein-single stranded DNA complexes. Modulation by NaCl concentration. J Biol Chem (1985) 3.06

The bacterial RecA protein and the recombinational DNA repair of stalled replication forks. Annu Rev Biochem (2001) 3.03

Gene conversion involving the pilin structural gene correlates with pilus+ in equilibrium with pilus- changes in Neisseria gonorrhoeae. Cell (1986) 2.98

Direct observation of individual RecA filaments assembling on single DNA molecules. Nature (2006) 2.55

Antigenic variation of pilin regulates adhesion of Neisseria meningitidis to human epithelial cells. Mol Microbiol (1993) 2.44

Effects of recA mutations on pilus antigenic variation and phase transitions in Neisseria gonorrhoeae. Genetics (1987) 2.39

Regulation of bacterial RecA protein function. Crit Rev Biochem Mol Biol (2007) 2.37

Cloning of the recA gene of Neisseria gonorrhoeae and construction of gonococcal recA mutants. J Bacteriol (1987) 2.23

Function of nucleoside triphosphate and polynucleotide in Escherichia coli recA protein-directed cleavage of phage lambda repressor. J Biol Chem (1981) 2.00

Recombination proteins and rescue of arrested replication forks. DNA Repair (Amst) (2007) 1.88

RecA protein filaments: end-dependent dissociation from ssDNA and stabilization by RecO and RecR proteins. J Mol Biol (1997) 1.87

Insertionally inactivated and inducible recA alleles for use in Neisseria. Gene (1997) 1.85

The direction of RecA protein assembly onto single strand DNA is the same as the direction of strand assimilation during strand exchange. J Biol Chem (1985) 1.78

The recX gene potentiates homologous recombination in Neisseria gonorrhoeae. Mol Microbiol (2001) 1.54

Continuous association of Escherichia coli single-stranded DNA binding protein with stable complexes of recA protein and single-stranded DNA. Biochemistry (1986) 1.54

Structural data suggest that the active and inactive forms of the RecA filament are not simply interconvertible. J Mol Biol (1992) 1.53

Efficacy trial of a parenteral gonococcal pilus vaccine in men. Vaccine (1991) 1.50

Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo. J Biol Chem (2002) 1.49

Recombination, repair and replication in the pathogenic Neisseriae: the 3 R's of molecular genetics of two human-specific bacterial pathogens. Mol Microbiol (2003) 1.48

A genetic screen identifies genes and sites involved in pilin antigenic variation in Neisseria gonorrhoeae. Mol Microbiol (2005) 1.47

Assembly and disassembly of RecA protein filaments occur at opposite filament ends. Relationship to DNA strand exchange. J Biol Chem (1990) 1.42

The nonmutagenic repair of broken replication forks via recombination. Mutat Res (2002) 1.41

Mycobacterial recA is cotranscribed with a potential regulatory gene called recX. Mol Microbiol (1997) 1.37

A RecA filament capping mechanism for RecX protein. Mol Cell (2004) 1.34

recA protein-promoted ATP hydrolysis occurs throughout recA nucleoprotein filaments. J Biol Chem (1987) 1.23

Dynamics of RecA filaments on single-stranded DNA. Nucleic Acids Res (2009) 1.23

RuvB protein-mediated ATP hydrolysis: functional asymmetry in the RuvB hexamer. Biochemistry (1995) 1.22

C-terminal deletions of the Escherichia coli RecA protein. Characterization of in vivo and in vitro effects. J Biol Chem (2003) 1.22

Effects of overproduction of single-stranded DNA-binding protein on RecA protein-dependent processes in Escherichia coli. J Mol Biol (1987) 1.20

Situational repair of replication forks: roles of RecG and RecA proteins. J Biol Chem (2003) 1.20

Exchange of recA protein between adjacent recA protein-single-stranded DNA complexes. J Biol Chem (1986) 1.19

Quantitative analysis of the kinetics of end-dependent disassembly of RecA filaments from ssDNA. J Mol Biol (1999) 1.18

Transcriptional and mutational analyses of the Streptomyces lividans recX gene and its interference with RecA activity. J Bacteriol (2000) 1.08

Absence of an SOS-like system in Neisseria gonorrhoeae. Gene (1998) 1.05

RecA-mediated SOS induction requires an extended filament conformation but no ATP hydrolysis. Mol Microbiol (2008) 1.04

Homology-dependent changes in adenosine 5'-triphosphate hydrolysis during recA protein promoted DNA strand exchange: evidence for long paranemic complexes. Biochemistry (1987) 1.02

recX, a new SOS gene that is co-transcribed with the recA gene in Escherichia coli. DNA Repair (Amst) (2003) 1.00

Inhibition of RecA protein by the Escherichia coli RecX protein: modulation by the RecA C terminus and filament functional state. J Biol Chem (2004) 0.99

Complexes of RecA with LexA and RecX differentiate between active and inactive RecA nucleoprotein filaments. J Mol Biol (2003) 0.98

A putative regulatory gene downstream of recA is conserved in gram-negative and gram-positive bacteria. Nucleic Acids Res (1994) 0.96

An SOS inhibitor that binds to free RecA protein: the PsiB protein. Mol Cell (2009) 0.94

Structural basis for inhibition of homologous recombination by the RecX protein. EMBO J (2008) 0.92

Differential cross-complementation patterns of Escherichia coli and Neisseria gonorrhoeae RecA proteins. Microbiology (2002) 0.87

SSB antagonizes RecX-RecA interaction. J Biol Chem (2008) 0.83

New insights into protein-DNA interactions obtained by electron microscopy. Mol Biotechnol (2000) 0.79

Articles by these authors

SSB as an organizer/mobilizer of genome maintenance complexes. Crit Rev Biochem Mol Biol (2008) 3.19

The bacterial RecA protein and the recombinational DNA repair of stalled replication forks. Annu Rev Biochem (2001) 3.03

Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis. Mol Cell (2013) 2.52

Circadian clock protein KaiC forms ATP-dependent hexameric rings and binds DNA. Proc Natl Acad Sci U S A (2002) 2.50

An alternative DNA structure is necessary for pilin antigenic variation in Neisseria gonorrhoeae. Science (2009) 2.37

The obligate human pathogen, Neisseria gonorrhoeae, is polyploid. PLoS Biol (2006) 1.84

Transcription of a cis-acting, noncoding, small RNA is required for pilin antigenic variation in Neisseria gonorrhoeae. PLoS Pathog (2013) 1.72

The frequency and rate of pilin antigenic variation in Neisseria gonorrhoeae. Mol Microbiol (2005) 1.65

Roles of the recJ and recN genes in homologous recombination and DNA repair pathways of Neisseria gonorrhoeae. J Bacteriol (2002) 1.64

The transcriptome response of Neisseria gonorrhoeae to hydrogen peroxide reveals genes with previously uncharacterized roles in oxidative damage protection. Mol Microbiol (2005) 1.61

Directed evolution of ionizing radiation resistance in Escherichia coli. J Bacteriol (2009) 1.60

The outer membrane localization of the Neisseria gonorrhoeae MsrA/B is involved in survival against reactive oxygen species. Proc Natl Acad Sci U S A (2002) 1.55

Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo. J Biol Chem (2002) 1.49

Recombination, repair and replication in the pathogenic Neisseriae: the 3 R's of molecular genetics of two human-specific bacterial pathogens. Mol Microbiol (2003) 1.48

A genetic screen identifies genes and sites involved in pilin antigenic variation in Neisseria gonorrhoeae. Mol Microbiol (2005) 1.47

The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP. Nature (2009) 1.43

The penC mutation conferring antibiotic resistance in Neisseria gonorrhoeae arises from a mutation in the PilQ secretin that interferes with multimer stability. Mol Microbiol (2005) 1.41

SSB protein limits RecOR binding onto single-stranded DNA. J Biol Chem (2007) 1.35

RecA acts in trans to allow replication of damaged DNA by DNA polymerase V. Nature (2006) 1.34

A RecA filament capping mechanism for RecX protein. Mol Cell (2004) 1.34

Crystal structure of the Deinococcus radiodurans single-stranded DNA-binding protein suggests a mechanism for coping with DNA damage. Proc Natl Acad Sci U S A (2004) 1.33

Preserving genome integrity: the DdrA protein of Deinococcus radiodurans R1. PLoS Biol (2004) 1.31

Low-level pilin expression allows for substantial DNA transformation competence in Neisseria gonorrhoeae. Infect Immun (2003) 1.26

Organized unidirectional waves of ATP hydrolysis within a RecA filament. PLoS Biol (2005) 1.26

Resistance of Neisseria gonorrhoeae to non-oxidative killing by adherent human polymorphonuclear leucocytes. Cell Microbiol (2009) 1.24

Opportunity and means: horizontal gene transfer from the human host to a bacterial pathogen. MBio (2011) 1.23

DdrB protein, an alternative Deinococcus radiodurans SSB induced by ionizing radiation. J Biol Chem (2009) 1.23

Regulation of single-stranded DNA binding by the C termini of Escherichia coli single-stranded DNA-binding (SSB) protein. J Biol Chem (2010) 1.22

Evolution of extreme resistance to ionizing radiation via genetic adaptation of DNA repair. Elife (2014) 1.22

Neisseria gonorrhoeae suppresses the oxidative burst of human polymorphonuclear leukocytes. Cell Microbiol (2008) 1.22

C-terminal deletions of the Escherichia coli RecA protein. Characterization of in vivo and in vitro effects. J Biol Chem (2003) 1.22

Neisseria gonorrhoeae DNA recombination and repair enzymes protect against oxidative damage caused by hydrogen peroxide. J Bacteriol (2006) 1.21

A mutant form of the Neisseria gonorrhoeae pilus secretin protein PilQ allows increased entry of heme and antimicrobial compounds. J Bacteriol (2004) 1.20

Situational repair of replication forks: roles of RecG and RecA proteins. J Biol Chem (2003) 1.20

The DinI protein stabilizes RecA protein filaments. J Biol Chem (2004) 1.18

Microbial antigenic variation mediated by homologous DNA recombination. FEMS Microbiol Rev (2012) 1.18

The DinI and RecX proteins are competing modulators of RecA function. J Biol Chem (2004) 1.17

DNA polymerase V and RecA protein, a minimal mutasome. Mol Cell (2005) 1.16

The Rad51-dependent pairing of long DNA substrates is stabilized by replication protein A. J Biol Chem (2002) 1.16

Loss of both Holliday junction processing pathways is synthetically lethal in the presence of gonococcal pilin antigenic variation. Mol Microbiol (2006) 1.15

The C terminus of the Escherichia coli RecA protein modulates the DNA binding competition with single-stranded DNA-binding protein. J Biol Chem (2003) 1.15

A bacterial siren song: intimate interactions between Neisseria and neutrophils. Nat Rev Microbiol (2012) 1.14

DNA uptake sequence-mediated enhancement of transformation in Neisseria gonorrhoeae is strain dependent. J Bacteriol (2010) 1.14

Role of the Rep helicase gene in homologous recombination in Neisseria gonorrhoeae. J Bacteriol (2005) 1.13

Mutation of the priA gene of Neisseria gonorrhoeae affects DNA transformation and DNA repair. J Bacteriol (2005) 1.12

RecFOR and RecOR as distinct RecA loading pathways. J Biol Chem (2008) 1.11

Foundational concepts and underlying theories for majors in "biochemistry and molecular biology". Biochem Mol Biol Educ (2013) 1.09

A new model for SOS-induced mutagenesis: how RecA protein activates DNA polymerase V. Crit Rev Biochem Mol Biol (2010) 1.08

Magnesium ion-dependent activation of the RecA protein involves the C terminus. J Biol Chem (2003) 1.08

Defending science education against intelligent design: a call to action. J Clin Invest (2006) 1.07

The RecA proteins of Deinococcus radiodurans and Escherichia coli promote DNA strand exchange via inverse pathways. Proc Natl Acad Sci U S A (2002) 1.07

RecA Protein from the extremely radioresistant bacterium Deinococcus radiodurans: expression, purification, and characterization. J Bacteriol (2002) 1.06

The RecF protein antagonizes RecX function via direct interaction. Mol Cell (2006) 1.05

Transposon mutagenesis identifies sites upstream of the Neisseria gonorrhoeae pilE gene that modulate pilin antigenic variation. J Bacteriol (2007) 1.04

RecA-mediated SOS induction requires an extended filament conformation but no ATP hydrolysis. Mol Microbiol (2008) 1.04

A DNA pairing-enhanced conformation of bacterial RecA proteins. J Biol Chem (2003) 1.03

Inhibition of RecA protein function by the RdgC protein from Escherichia coli. J Biol Chem (2005) 1.02

A real-time semi-quantitative RT-PCR assay demonstrates that the pilE sequence dictates the frequency and characteristics of pilin antigenic variation in Neisseria gonorrhoeae. Nucleic Acids Res (2005) 1.02

Focusing homologous recombination: pilin antigenic variation in the pathogenic Neisseria. Mol Microbiol (2011) 1.01

Inhibition of RecA protein by the Escherichia coli RecX protein: modulation by the RecA C terminus and filament functional state. J Biol Chem (2004) 0.99

Complexes of RecA with LexA and RecX differentiate between active and inactive RecA nucleoprotein filaments. J Mol Biol (2003) 0.98

Binding of the dimeric Deinococcus radiodurans single-stranded DNA binding protein to single-stranded DNA. Biochemistry (2010) 0.98

Structure and cellular dynamics of Deinococcus radiodurans single-stranded DNA (ssDNA)-binding protein (SSB)-DNA complexes. J Biol Chem (2012) 0.97

Roles of DNA polymerase V and RecA protein in SOS damage-induced mutation. Chem Rev (2006) 0.97

Frequency and rate of pilin antigenic variation of Neisseria meningitidis. J Bacteriol (2010) 0.96

Pilin antigenic variation occurs independently of the RecBCD pathway in Neisseria gonorrhoeae. J Bacteriol (2009) 0.96

The deinococcal DdrB protein is involved in an early step of DNA double strand break repair and in plasmid transformation through its single-strand annealing activity. DNA Repair (Amst) (2011) 0.95

Polar destabilization of DNA duplexes with single-stranded overhangs by the Deinococcus radiodurans SSB protein. Biochemistry (2006) 0.94

An SOS inhibitor that binds to free RecA protein: the PsiB protein. Mol Cell (2009) 0.94

RecA-binding pilE G4 sequence essential for pilin antigenic variation forms monomeric and 5' end-stacked dimeric parallel G-quadruplexes. Structure (2012) 0.94

pilQ Missense mutations have diverse effects on PilQ multimer formation, piliation, and pilus function in Neisseria gonorrhoeae. J Bacteriol (2007) 0.93

Gonococci exit apically and basally from polarized epithelial cells and exhibit dynamic changes in type IV pili. Cell Microbiol (2006) 0.92

Complementation of one RecA protein point mutation by another. Evidence for trans catalysis of ATP hydrolysis. J Biol Chem (2006) 0.92

Genomic content of Neisseria species. J Bacteriol (2010) 0.92

Neisseria gonorrhoeae-mediated inhibition of apoptotic signalling in polymorphonuclear leukocytes. Infect Immun (2011) 0.91

Analysis of the Piv recombinase-related gene family of Neisseria gonorrhoeae. J Bacteriol (2005) 0.91

Two distinct modes of RecA action are required for DNA polymerase V-catalyzed translesion synthesis. Proc Natl Acad Sci U S A (2002) 0.89

The genetics of Neisseria species. Annu Rev Genet (2014) 0.89

Mismatch repair inhibits homeologous recombination via coordinated directional unwinding of trapped DNA structures. Mol Cell (2013) 0.89

The Deinococcus radiodurans DR1245 protein, a DdrB partner homologous to YbjN proteins and reminiscent of type III secretion system chaperones. PLoS One (2013) 0.89

Regulation of Deinococcus radiodurans RecA protein function via modulation of active and inactive nucleoprotein filament states. J Biol Chem (2013) 0.88

Defective dissociation of a "slow" RecA mutant protein imparts an Escherichia coli growth defect. J Biol Chem (2008) 0.88

Differential cross-complementation patterns of Escherichia coli and Neisseria gonorrhoeae RecA proteins. Microbiology (2002) 0.87

Mismatch correction modulates mutation frequency and pilus phase and antigenic variation in Neisseria gonorrhoeae. J Bacteriol (2010) 0.87

Developing single-molecule TPM experiments for direct observation of successful RecA-mediated strand exchange reaction. PLoS One (2011) 0.86