Quantitative analysis of the binding of ezrin to large unilamellar vesicles containing phosphatidylinositol 4,5 bisphosphate.

PubWeight™: 1.04‹?› | Rank: Top 15%

🔗 View Article (PMC 2186265)

Published in Biophys J on September 07, 2007

Authors

Guillaume Blin1, Emmanuel Margeat, Kévin Carvalho, Catherine A Royer, Christian Roy, Catherine Picart

Author Affiliations

1: DIMNP, Universités Montpellier II et I, CNRS, Montpellier cedex 05, France.

Articles citing this

Inhibition of the kinase Csk in thymocytes reveals a requirement for actin remodeling in the initiation of full TCR signaling. Nat Immunol (2013) 1.61

Cytoskeleton-membrane interactions in membrane raft structure. Cell Mol Life Sci (2009) 1.59

Giant unilamellar vesicles containing phosphatidylinositol(4,5)bisphosphate: characterization and functionality. Biophys J (2008) 1.31

Phosphatidylinositol-(4,5)-bisphosphate enables efficient secretion of HIV-1 Tat by infected T-cells. EMBO J (2010) 1.18

Targeting of murine leukemia virus gag to the plasma membrane is mediated by PI(4,5)P2/PS and a polybasic region in the matrix. J Virol (2010) 1.17

Structural basis of phosphoinositide binding to kindlin-2 protein pleckstrin homology domain in regulating integrin activation. J Biol Chem (2011) 1.12

ADF/cofilin binds phosphoinositides in a multivalent manner to act as a PIP(2)-density sensor. Biophys J (2010) 1.12

Pathogen destruction versus intracellular survival: the role of lipids as phagosomal fate determinants. J Clin Invest (2008) 1.10

Isolation, characterization and lipid-binding properties of the recalcitrant FtsA division protein from Escherichia coli. PLoS One (2012) 0.97

Activation of moesin, a protein that links actin cytoskeleton to the plasma membrane, occurs by phosphatidylinositol 4,5-bisphosphate (PIP2) binding sequentially to two sites and releasing an autoinhibitory linker. J Biol Chem (2012) 0.93

Phosphoinositide binding differentially regulates NHE1 Na+/H+ exchanger-dependent proximal tubule cell survival. J Biol Chem (2011) 0.88

Characterization of the control catabolite protein of gluconeogenic genes repressor by fluorescence cross-correlation spectroscopy and other biophysical approaches. Biophys J (2008) 0.81

Counterion-mediated pattern formation in membranes containing anionic lipids. Adv Colloid Interface Sci (2014) 0.80

FERM domain of moesin desorbs the basic-rich cytoplasmic domain of l-selectin from the anionic membrane surface. J Mol Biol (2013) 0.80

Model membranes to shed light on the biochemical and physical properties of ezrin/radixin/moesin. Biochimie (2012) 0.79

Binding of moesin and ezrin to membranes containing phosphatidylinositol (4,5) bisphosphate: a comparative study of the affinity constants and conformational changes. Biochim Biophys Acta (2012) 0.79

Evidence That Bacteriophage λ Kil Peptide Inhibits Bacterial Cell Division by Disrupting FtsZ Protofilaments and Sequestering Protein Subunits. J Biol Chem (2015) 0.77

Phosphoinositide Binding Inhibits Actin Crosslinking and Polymerization by Palladin. J Mol Biol (2016) 0.77

Mode of Ezrin-Membrane Interaction as a Function of PIP2 Binding and Pseudophosphorylation. Biophys J (2016) 0.75

Application of Peak Intensity Analysis to Measurements of Protein Binding to Lipid Vesicles and Erythrocytes Using Fluorescence Correlation Spectroscopy: Dependence on Particle Size. J Membr Biol (2016) 0.75

Articles cited by this

ERM proteins and merlin: integrators at the cell cortex. Nat Rev Mol Cell Biol (2002) 8.41

PIP(2) and proteins: interactions, organization, and information flow. Annu Rev Biophys Biomol Struct (2001) 5.66

The electrostatic properties of membranes. Annu Rev Biophys Biophys Chem (1989) 5.37

Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase. Nature (2002) 5.13

Phosphoinositide regulation of the actin cytoskeleton. Annu Rev Physiol (2002) 4.56

Production of large unilamellar vesicles by a rapid extrusion procedure: characterization of size distribution, trapped volume and ability to maintain a membrane potential. Biochim Biophys Acta (1985) 4.47

Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4,5-bisphosphate-dependent organization of cell actin. Proc Natl Acad Sci U S A (2003) 4.34

Reassembly of contractile actin cortex in cell blebs. J Cell Biol (2006) 4.31

Structure of the ERM protein moesin reveals the FERM domain fold masked by an extended actin binding tail domain. Cell (2000) 3.94

Ezrin self-association involves binding of an N-terminal domain to a normally masked C-terminal domain that includes the F-actin binding site. Mol Biol Cell (1995) 3.36

Cell spreading and lamellipodial extension rate is regulated by membrane tension. J Cell Biol (2000) 3.18

Phosphoinositide binding and phosphorylation act sequentially in the activation mechanism of ezrin. J Cell Biol (2004) 3.03

A polybasic motif allows N-WASP to act as a sensor of PIP(2) density. Mol Cell (2005) 2.80

Structure of the binding site for inositol phosphates in a PH domain. EMBO J (1995) 2.71

ERM proteins in cell adhesion and membrane dynamics. Trends Cell Biol (1999) 2.71

Electrostatic sequestration of PIP2 on phospholipid membranes by basic/aromatic regions of proteins. Biophys J (2004) 2.66

Cortical actin organization: lessons from ERM (ezrin/radixin/moesin) proteins. J Biol Chem (1999) 2.60

Receptor-mediated regulation of PI3Ks confines PI(3,4,5)P3 to the leading edge of chemotaxing cells. Mol Biol Cell (2003) 2.56

Structural basis of the membrane-targeting and unmasking mechanisms of the radixin FERM domain. EMBO J (2000) 2.49

Phosphatidylinositol-3,4,5-trisphosphate regulates the formation of the basolateral plasma membrane in epithelial cells. Nat Cell Biol (2006) 2.39

Actin polymerization serves as a membrane domain switch in model lipid bilayers. Biophys J (2006) 2.00

Mutagenesis of the phosphatidylinositol 4,5-bisphosphate (PIP(2)) binding site in the NH(2)-terminal domain of ezrin correlates with its altered cellular distribution. J Cell Biol (2000) 1.97

Cytoskeletal regulation: rich in lipids. Nat Rev Mol Cell Biol (2004) 1.94

Direct involvement of ezrin/radixin/moesin (ERM)-binding membrane proteins in the organization of microvilli in collaboration with activated ERM proteins. J Cell Biol (1999) 1.91

Identification of a phosphatidylinositol-4,5-bisphosphate-binding domain in the N-terminal region of ezrin. FEBS Lett (1995) 1.89

Inositol-lipid binding motifs: signal integrators through protein-lipid and protein-protein interactions. J Cell Sci (2005) 1.88

Phosphoinositide-specific phospholipase C-delta 1 binds with high affinity to phospholipid vesicles containing phosphatidylinositol 4,5-bisphosphate. Biochemistry (1992) 1.82

Myo1c binds phosphoinositides through a putative pleckstrin homology domain. Mol Biol Cell (2006) 1.74

Fluorescence correlation spectroscopy measures molecular transport in cells. Traffic (2001) 1.74

Myo1c binds tightly and specifically to phosphatidylinositol 4,5-bisphosphate and inositol 1,4,5-trisphosphate. Proc Natl Acad Sci U S A (2006) 1.72

Differential roles of ionic, aliphatic, and aromatic residues in membrane-protein interactions: a surface plasmon resonance study on phospholipases A2. Biochemistry (2001) 1.69

Essential functions of ezrin in maintenance of cell shape and lamellipodial extension in normal and transformed fibroblasts. Curr Biol (1997) 1.68

Differential regulation of phagosome maturation in macrophages and dendritic cells mediated by Rho GTPases and ezrin-radixin-moesin (ERM) proteins. Proc Natl Acad Sci U S A (2006) 1.64

Phosphoinositides: key players in cell signalling, in time and space. Cell Signal (2001) 1.57

Chemokine stimulation of human peripheral blood T lymphocytes induces rapid dephosphorylation of ERM proteins, which facilitates loss of microvilli and polarization. Blood (2003) 1.50

C-terminal threonine phosphorylation activates ERM proteins to link the cell's cortical lipid bilayer to the cytoskeleton. Biochem Biophys Res Commun (1998) 1.50

Adsorption of cations to phosphatidylinositol 4,5-bisphosphate. Biochemistry (1988) 1.49

Fluorescence correlation spectroscopy studies of Peptide and protein binding to phospholipid vesicles. Biophys J (2004) 1.47

Membrane binding assays for peripheral proteins. Anal Biochem (2001) 1.45

Membrane-bound basic peptides sequester multivalent (PIP2), but not monovalent (PS), acidic lipids. Biophys J (2006) 1.40

A dual involvement of the amino-terminal domain of ezrin in F- and G-actin binding. J Biol Chem (1997) 1.39

Phosphoinositide metabolism during membrane ruffling and macropinosome formation in EGF-stimulated A431 cells. Exp Cell Res (2007) 1.38

Dynamics of cytoskeletal proteins during Fcgamma receptor-mediated phagocytosis in macrophages. Mol Biol Cell (2002) 1.36

Phosphoinositide signaling disorders in human diseases. FEBS Lett (2003) 1.29

Phosphoinositide specificity of and mechanism of lipid domain formation by annexin A2-p11 heterotetramer. J Biol Chem (2005) 1.28

Phosphoinositides regulate membrane-dependent actin assembly by latex bead phagosomes. Mol Biol Cell (2002) 1.25

Leading-edge research: PtdIns(3,4,5)P3 and directed migration. Nat Cell Biol (2007) 1.20

Regulation of protein activities by phosphoinositide phosphates. Annu Rev Cell Dev Biol (2005) 1.18

Ultracentrifugation technique for measuring the binding of peptides and proteins to sucrose-loaded phospholipid vesicles. Methods Mol Biol (1998) 1.16

Cooperative adsorption of ezrin on PIP2-containing membranes. Biochemistry (2006) 1.05

pH-dependent domain formation in phosphatidylinositol polyphosphate/phosphatidylcholine mixed vesicles. J Lipid Res (2004) 1.05

Membrane cytoskeleton: PIP(2) pulls the strings. Curr Biol (2000) 0.97

Fluorescence correlation spectroscopy analysis of the hydrophobic interactions of protein 4.1 with phosphatidyl serine liposomes. Biophys Chem (1999) 0.96

Localised PtdIns(3,4,5)P3 or PtdIns(3,4)P2 at the phagocytic cup is required for both phagosome closure and Ca2+ signalling in HL60 neutrophils. J Cell Sci (2006) 0.94

Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine. J Lipid Res (2006) 0.93

Structure of molecular aggregates of 1-(3-sn-phosphatidyl)-L-myo-inositol 3,4-bis(phosphate) in water. Biochim Biophys Acta (1981) 0.91

Phospholipid binding of synthetic talin peptides provides evidence for an intrinsic membrane anchor of talin. J Biol Chem (2000) 0.87

Asymmetric ERM activation at the Schwann cell process tip is required in axon-associated motility. J Cell Physiol (2007) 0.86

A role for moesin in polarity. Trends Cell Biol (2003) 0.85

Opening of holes in liposomal membranes is induced by proteins possessing the FERM domain. J Mol Biol (2006) 0.82

Articles by these authors

Carbocyanine dyes as efficient reversible single-molecule optical switch. J Am Chem Soc (2005) 5.56

Initial transcription by RNA polymerase proceeds through a DNA-scrunching mechanism. Science (2006) 4.36

Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules. Proc Natl Acad Sci U S A (2004) 4.06

Phosphoinositide binding and phosphorylation act sequentially in the activation mechanism of ezrin. J Cell Biol (2004) 3.03

Alternating-laser excitation of single molecules. Acc Chem Res (2005) 2.25

Multiple functionalities of polyelectrolyte multilayer films: new biomedical applications. Adv Mater (2010) 2.22

Retention of transcription initiation factor sigma70 in transcription elongation: single-molecule analysis. Mol Cell (2005) 1.96

Dynamic partitioning of a glycosyl-phosphatidylinositol-anchored protein in glycosphingolipid-rich microdomains imaged by single-quantum dot tracking. Traffic (2009) 1.83

Improvement of stability and cell adhesion properties of polyelectrolyte multilayer films by chemical cross-linking. Biomacromolecules (2004) 1.73

The H-NS dimerization domain defines a new fold contributing to DNA recognition. Nat Struct Biol (2003) 1.61

Cavities determine the pressure unfolding of proteins. Proc Natl Acad Sci U S A (2012) 1.52

Modulators of the structural dynamics of the retinoid X receptor to reveal receptor function. Proc Natl Acad Sci U S A (2007) 1.48

Characterization of the interaction between retinoic acid receptor/retinoid X receptor (RAR/RXR) heterodimers and transcriptional coactivators through structural and fluorescence anisotropy studies. J Biol Chem (2004) 1.44

Polyelectrolyte multilayers with a tunable Young's modulus: influence of film stiffness on cell adhesion. Langmuir (2006) 1.42

An extended relationship for the characterization of Young's modulus and Poisson's ratio of tunable polyacrylamide gels. Biorheology (2006) 1.40

MabA (FabG1), a Mycobacterium tuberculosis protein involved in the long-chain fatty acid elongation system FAS-II. Microbiology (2002) 1.36

Giant unilamellar vesicles containing phosphatidylinositol(4,5)bisphosphate: characterization and functionality. Biophys J (2008) 1.31

Elasticity of native and cross-linked polyelectrolyte multilayer films. Biomacromolecules (2004) 1.30

Layer-by-layer films as a biomimetic reservoir for rhBMP-2 delivery: controlled differentiation of myoblasts to osteoblasts. Small (2009) 1.29

Single-molecule analysis of CD9 dynamics and partitioning reveals multiple modes of interaction in the tetraspanin web. J Cell Biol (2008) 1.29

Phosphoinositides regulate membrane-dependent actin assembly by latex bead phagosomes. Mol Biol Cell (2002) 1.25

Polyelectrolyte multilayer films of controlled stiffness modulate myoblast cells differentiation. Adv Funct Mater (2008) 1.20

Phosphatidylinositol-(4,5)-bisphosphate enables efficient secretion of HIV-1 Tat by infected T-cells. EMBO J (2010) 1.18

Phosphatidylinositol 3-phosphate, an essential lipid in Plasmodium, localizes to the food vacuole membrane and the apicoplast. Eukaryot Cell (2010) 1.16

The performance of BMP-2 loaded TCP/HAP porous ceramics with a polyelectrolyte multilayer film coating. Biomaterials (2011) 1.16

Differential action on coregulator interaction defines inverse retinoid agonists and neutral antagonists. Chem Biol (2009) 1.14

Layer by layer buildup of polysaccharide films: physical chemistry and cellular adhesion aspects. Langmuir (2004) 1.12

HIV-1 assembly differentially alters dynamics and partitioning of tetraspanins and raft components. Traffic (2010) 1.11

An extended modeling of the micropipette aspiration experiment for the characterization of the Young's modulus and Poisson's ratio of adherent thin biological samples: numerical and experimental studies. J Biomech (2005) 1.09

Degradability of polysaccharides multilayer films in the oral environment: an in vitro and in vivo study. Biomacromolecules (2005) 1.06

A stepwise 2'-hydroxyl activation mechanism for the bacterial transcription termination factor Rho helicase. Nat Struct Mol Biol (2009) 1.06

Presentation of BMP-2 from a soft biopolymeric film unveils its activity on cell adhesion and migration. Adv Mater (2011) 1.06

The nuclear receptor coactivator PGC-1alpha exhibits modes of interaction with the estrogen receptor distinct from those of SRC-1. J Mol Biol (2005) 1.05

Rck of Salmonella enterica, subspecies enterica serovar enteritidis, mediates zipper-like internalization. Cell Res (2010) 1.03

Double molecular mimicry in Escherichia coli: binding of ribosomal protein L20 to its two sites in mRNA is similar to its binding to 23S rRNA. Mol Microbiol (2005) 1.03

Cytotoxicity of polyethyleneimine (PEI), precursor base layer of polyelectrolyte multilayer films. Biomaterials (2006) 1.03

Differential roles of PtdIns(4,5)P2 and phosphorylation in moesin activation during Drosophila development. J Cell Sci (2010) 1.02

Reduced adipose tissue triglyceride synthesis and increased muscle fatty acid oxidation in C5L2 knockout mice. J Endocrinol (2007) 1.02

Variation of polyelectrolyte film stiffness by photo-cross-linking: a new way to control cell adhesion. Langmuir (2009) 1.00

Size and sequence and the volume change of protein folding. J Am Chem Soc (2011) 1.00

Natural polyelectrolyte films based on layer-by layer deposition of collagen and hyaluronic acid. Biomaterials (2005) 1.00

A unique secondary-structure switch controls constitutive gene repression by retinoic acid receptor. Nat Struct Mol Biol (2010) 0.99

Nonlinear elastic properties of polyacrylamide gels: implications for quantification of cellular forces. Biorheology (2009) 0.99

Phosphatidylinositol 3-monophosphate is involved in toxoplasma apicoplast biogenesis. PLoS Pathog (2011) 0.99

Structure-based discovery of an inhibitor of Arf activation by Sec7 domains through targeting of protein-protein complexes. Proc Natl Acad Sci U S A (2007) 0.99

Multifunctional polyelectrolyte multilayer films: combining mechanical resistance, biodegradability, and bioactivity. Biomacromolecules (2007) 0.98

Membrane targeting of protein tyrosine phosphatase PTPL1 through its FERM domain via binding to phosphatidylinositol 4,5-biphosphate. J Cell Sci (2003) 0.98

Mutagenesis-based evidence for an asymmetric configuration of the ring-shaped transcription termination factor Rho. J Mol Biol (2010) 0.96

Effect of crosslinking on the elasticity of polyelectrolyte multilayer films measured by colloidal probe AFM. Microsc Res Tech (2006) 0.95

Inducer-modulated cooperative binding of the tetrameric CggR repressor to operator DNA. Biophys J (2007) 0.94

Imaging cell interactions with native and crosslinked polyelectrolyte multilayers. Cell Biochem Biophys (2006) 0.94

V(i)-value analysis: a pressure-based method for mapping the folding transition state ensemble of proteins. J Am Chem Soc (2007) 0.94

Recruitment, assembly, and molecular architecture of the SpoIIIE DNA pump revealed by superresolution microscopy. PLoS Biol (2013) 0.94

Unique features of the folding landscape of a repeat protein revealed by pressure perturbation. Biophys J (2010) 0.93

Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states. Proc Natl Acad Sci U S A (2011) 0.93

Activation of moesin, a protein that links actin cytoskeleton to the plasma membrane, occurs by phosphatidylinositol 4,5-bisphosphate (PIP2) binding sequentially to two sites and releasing an autoinhibitory linker. J Biol Chem (2012) 0.93

Identification of a novel Ezrin-binding site in syndecan-2 cytoplasmic domain. FEBS Lett (2003) 0.92

Keeping up to speed with the transcription termination factor Rho motor. Transcription (2012) 0.92

The stability of BMP loaded polyelectrolyte multilayer coatings on titanium. Biomaterials (2013) 0.92

Effects of chaotropic and kosmotropic cosolvents on the pressure-induced unfolding and denaturation of proteins: an FT-IR study on staphylococcal nuclease. Biochemistry (2004) 0.90

Ion pairing and hydration in polyelectrolyte multilayer films containing polysaccharides. Biomacromolecules (2009) 0.90

Transcription termination factor rho can displace streptavidin from biotinylated RNA. J Biol Chem (2007) 0.90

Polysaccharide-blend multilayers containing hyaluronan and heparin as a delivery system for rhBMP-2. Small (2010) 0.89

Effect of internal cavities on folding rates and routes revealed by real-time pressure-jump NMR spectroscopy. J Am Chem Soc (2013) 0.89

Towards a quantitative understanding of protein hydration and volumetric properties. Chemphyschem (2008) 0.89

A 24 bp cis-acting element essential for the transcriptional activity of Plasmodium falciparum CDP-diacylglycerol synthase gene promoter. Mol Biochem Parasitol (2002) 0.88

Structural insight into the Mycobacterium tuberculosis Rv0020c protein and its interaction with the PknB kinase. Structure (2011) 0.87

Elasticity, biodegradability and cell adhesive properties of chitosan/hyaluronan multilayer films. Biomed Mater (2007) 0.87

Glycated polyelectrolyte multilayer films: differential adhesion of primary versus tumor cells. Biomacromolecules (2006) 0.87

How actin network dynamics control the onset of actin-based motility. Proc Natl Acad Sci U S A (2012) 0.87

Intestinally derived lipids: metabolic regulation and consequences--an overview. Atheroscler Suppl (2008) 0.87

Internal composition versus the mechanical properties of polyelectrolyte multilayer films: the influence of chemical cross-linking. Langmuir (2009) 0.87

SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation. EMBO Rep (2013) 0.87

Probing the physical determinants of thermal expansion of folded proteins. J Phys Chem B (2013) 0.87

Absolute quantification of gene expression in individual bacterial cells using two-photon fluctuation microscopy. Anal Biochem (2011) 0.86

Quantitative detection of the ligand-dependent interaction between the androgen receptor and the co-activator, Tif2, in live cells using two color, two photon fluorescence cross-correlation spectroscopy. Eur Biophys J (2006) 0.86

Manipulation of the adhesive behaviour of skeletal muscle cells on soft and stiff polyelectrolyte multilayers. Acta Biomater (2010) 0.86

Remodeling of the folding free energy landscape of staphylococcal nuclease by cavity-creating mutations. Biochemistry (2012) 0.86

Effect of RGD functionalization and stiffness modulation of polyelectrolyte multilayer films on muscle cell differentiation. Acta Biomater (2012) 0.86

Engineering muscle tissues on microstructured polyelectrolyte multilayer films. Tissue Eng Part A (2012) 0.86

Surface functionalization of hyaluronic acid hydrogels by polyelectrolyte multilayer films. Biomaterials (2011) 0.86

Self assembling and crosslinking of polyelectrolyte multilayer films of chitosan and alginate studied by QCM and IR spectroscopy. Macromol Biosci (2009) 0.86

Estrogen receptor interactions and dynamics monitored in live cells by fluorescence cross-correlation spectroscopy. Biochemistry (2010) 0.85

Acylation-stimulating protein/C5L2-neutralizing antibodies alter triglyceride metabolism in vitro and in vivo. Am J Physiol Endocrinol Metab (2007) 0.84

Free-standing polyelectrolyte membranes made of chitosan and alginate. Biomacromolecules (2013) 0.84

Negative regulation of estrogen signaling by ERβ and RIP140 in ovarian cancer cells. Mol Endocrinol (2013) 0.84

pH-Amplified multilayer films based on hyaluronan: influence of HA molecular weight and concentration on film growth and stability. Biomacromolecules (2011) 0.84

Fructose-1,6-bisphosphate acts both as an inducer and as a structural cofactor of the central glycolytic genes repressor (CggR). Biochemistry (2007) 0.84

RAR antagonists diminish the level of DNA binding by the RAR/RXR heterodimer. Biochemistry (2003) 0.83

Dynamics of poly(L-lysine) in hyaluronic acid/poly(L-lysine) multilayer films studied by fluorescence recovery after pattern photobleaching. Langmuir (2008) 0.83

Layer-by-layer films from hyaluronan and amine-modified hyaluronan. Langmuir (2007) 0.83

A new effector of lipid metabolism: complement factor properdin. Mol Immunol (2012) 0.83