Published in Nature on October 10, 2002
A practical guide to single-molecule FRET. Nat Methods (2008) 8.62
Analysis of single-molecule FRET trajectories using hidden Markov modeling. Biophys J (2006) 5.52
tRNA dynamics on the ribosome during translation. Proc Natl Acad Sci U S A (2004) 5.38
An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments. Biophys J (2007) 4.49
Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules. Proc Natl Acad Sci U S A (2004) 4.06
New directions in single-molecule imaging and analysis. Proc Natl Acad Sci U S A (2007) 3.83
RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP. Nature (2006) 3.67
A four-way junction accelerates hairpin ribozyme folding via a discrete intermediate. Proc Natl Acad Sci U S A (2003) 2.65
Extreme conformational diversity in human telomeric DNA. Proc Natl Acad Sci U S A (2005) 2.63
Vesicle encapsulation studies reveal that single molecule ribozyme heterogeneities are intrinsic. Biophys J (2004) 2.37
Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acids. Proc Natl Acad Sci U S A (2008) 2.24
Counting of six pRNAs of phi29 DNA-packaging motor with customized single-molecule dual-view system. EMBO J (2007) 2.21
Coordinating DNA replication by means of priming loop and differential synthesis rate. Nature (2009) 2.16
PcrA helicase dismantles RecA filaments by reeling in DNA in uniform steps. Cell (2010) 2.10
A nonuniform stepping mechanism for E. coli UvrD monomer translocation along single-stranded DNA. Mol Cell (2007) 2.00
Autoinhibition of Escherichia coli Rep monomer helicase activity by its 2B subdomain. Proc Natl Acad Sci U S A (2005) 1.95
Single-molecule studies of SNARE complex assembly reveal parallel and antiparallel configurations. Proc Natl Acad Sci U S A (2003) 1.94
Direct observation of abortive initiation and promoter escape within single immobilized transcription complexes. Biophys J (2005) 1.90
Sequence-dependent pausing of single lambda exonuclease molecules. Science (2003) 1.88
Forward and reverse motion of single RecBCD molecules on DNA. Biophys J (2004) 1.82
Real-time observation of strand exchange reaction with high spatiotemporal resolution. Structure (2011) 1.79
Revisiting the central dogma one molecule at a time. Cell (2011) 1.75
Interlaced optical force-fluorescence measurements for single molecule biophysics. Biophys J (2006) 1.75
General methods for analysis of sequential "n-step" kinetic mechanisms: application to single turnover kinetics of helicase-catalyzed DNA unwinding. Biophys J (2003) 1.70
Single-molecule studies highlight conformational heterogeneity in the early folding steps of a large ribozyme. Proc Natl Acad Sci U S A (2004) 1.69
Fueling protein DNA interactions inside porous nanocontainers. Proc Natl Acad Sci U S A (2007) 1.69
SSB functions as a sliding platform that migrates on DNA via reptation. Cell (2011) 1.53
Proliferating cell nuclear antigen uses two distinct modes to move along DNA. J Biol Chem (2009) 1.53
A fluorescence energy transfer-based mechanical stress sensor for specific proteins in situ. FEBS J (2008) 1.49
Cofilin-linked changes in actin filament flexibility promote severing. Biophys J (2011) 1.47
Conformational dynamics of single pre-mRNA molecules during in vitro splicing. Nat Struct Mol Biol (2010) 1.45
Single-molecule views of protein movement on single-stranded DNA. Annu Rev Biophys (2012) 1.39
DNA curtains and nanoscale curtain rods: high-throughput tools for single molecule imaging. Langmuir (2008) 1.39
Insights on the role of nucleic acid/protein interactions in chaperoned nucleic acid rearrangements of HIV-1 reverse transcription. Proc Natl Acad Sci U S A (2007) 1.37
HIV-1 Rev protein assembles on viral RNA one molecule at a time. Proc Natl Acad Sci U S A (2009) 1.36
Different quaternary structures of human RECQ1 are associated with its dual enzymatic activity. PLoS Biol (2007) 1.36
Development of new photon-counting detectors for single-molecule fluorescence microscopy. Philos Trans R Soc Lond B Biol Sci (2012) 1.32
Single-molecule analysis reveals differential effect of ssDNA-binding proteins on DNA translocation by XPD helicase. Mol Cell (2009) 1.32
Viral nanomotors for packaging of dsDNA and dsRNA. Mol Microbiol (2007) 1.31
Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave. Proc Natl Acad Sci U S A (2004) 1.30
Displacement of a DNA binding protein by Dda helicase. Nucleic Acids Res (2006) 1.30
Single-stranded DNA scanning and deamination by APOBEC3G cytidine deaminase at single molecule resolution. J Biol Chem (2012) 1.30
RNA looping by PTB: Evidence using FRET and NMR spectroscopy for a role in splicing repression. Proc Natl Acad Sci U S A (2010) 1.29
Single-pair FRET microscopy reveals mononucleosome dynamics. J Fluoresc (2007) 1.29
Probing the mechanisms of DEAD-box proteins as general RNA chaperones: the C-terminal domain of CYT-19 mediates general recognition of RNA. Biochemistry (2007) 1.27
Periodic DNA patrolling underlies diverse functions of Pif1 on R-loops and G-rich DNA. Elife (2014) 1.27
Single-molecule measurements of synthesis by DNA polymerase with base-pair resolution. Proc Natl Acad Sci U S A (2009) 1.27
DNA unwinding and protein displacement by superfamily 1 and superfamily 2 helicases. Nucleic Acids Res (2006) 1.26
Secondary structure and secondary structure dynamics of DNA hairpins complexed with HIV-1 NC protein. Biophys J (2004) 1.26
Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation at the single-molecule level. Proc Natl Acad Sci U S A (2009) 1.25
Transcription initiation by human RNA polymerase II visualized at single-molecule resolution. Genes Dev (2012) 1.24
BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation. EMBO J (2009) 1.21
Protein structure. Direct observation of structure-function relationship in a nucleic acid-processing enzyme. Science (2015) 1.21
Biological mechanisms, one molecule at a time. Genes Dev (2011) 1.19
Viewing single lambda site-specific recombination events from start to finish. EMBO J (2006) 1.19
The SOSS1 single-stranded DNA binding complex promotes DNA end resection in concert with Exo1. EMBO J (2012) 1.19
Single-molecule analysis of Mss116-mediated group II intron folding. Nature (2010) 1.17
Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding. EMBO J (2008) 1.16
A new view of protein synthesis: mapping the free energy landscape of the ribosome using single-molecule FRET. Biopolymers (2008) 1.16
Single-Vesicle Fusion Assay Reveals Munc18-1 Binding to the SNARE Core Is Sufficient for Stimulating Membrane Fusion. ACS Chem Neurosci (2010) 1.16
Single-molecule spectroscopic determination of lac repressor-DNA loop conformation. Biophys J (2005) 1.14
DNA mechanics as a tool to probe helicase and translocase activity. Nucleic Acids Res (2006) 1.13
Binding of pRNA to the N-terminal 14 amino acids of connector protein of bacteriophage phi29. Nucleic Acids Res (2005) 1.12
Structure-based model of the stepping motor of PcrA helicase. Biophys J (2006) 1.12
Novel mechanism of hexamer ring assembly in protein/RNA interactions revealed by single molecule imaging. Nucleic Acids Res (2008) 1.12
Single-molecule FRET of protein-nucleic acid and protein-protein complexes: surface passivation and immobilization. Methods (2010) 1.11
Holliday junction dynamics and branch migration: single-molecule analysis. Proc Natl Acad Sci U S A (2005) 1.10
Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding. Nucleic Acids Res (2008) 1.10
A prominent β-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation. Nucleic Acids Res (2010) 1.09
Measuring the folding transition time of single RNA molecules. Biophys J (2007) 1.09
Sequence-specific fluorescent labeling of double-stranded DNA observed at the single molecule level. Nucleic Acids Res (2003) 1.08
Staufen2 functions in Staufen1-mediated mRNA decay by binding to itself and its paralog and promoting UPF1 helicase but not ATPase activity. Proc Natl Acad Sci U S A (2012) 1.06
ATP-stimulated, DNA-mediated redox signaling by XPD, a DNA repair and transcription helicase. J Am Chem Soc (2011) 1.05
Quantitative analysis of the binding of ezrin to large unilamellar vesicles containing phosphatidylinositol 4,5 bisphosphate. Biophys J (2007) 1.04
Single-molecule FRET TACKLE reveals highly dynamic mismatched DNA-MutS complexes. Biochemistry (2010) 1.04
Detection of single DNA molecules by multicolor quantum-dot end-labeling. Nucleic Acids Res (2005) 1.04
Rotations of the 2B sub-domain of E. coli UvrD helicase/translocase coupled to nucleotide and DNA binding. J Mol Biol (2011) 1.04
Insight into helicase mechanism and function revealed through single-molecule approaches. Q Rev Biophys (2010) 1.03
The AddAB helicase-nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain. Nucleic Acids Res (2010) 1.02
Single-molecule FRET and linear dichroism studies of DNA breathing and helicase binding at replication fork junctions. Proc Natl Acad Sci U S A (2013) 1.01
Fifty years of DNA "Breathing": Reflections on old and new approaches. Biopolymers (2013) 0.99
Single molecule nanocontainers made porous using a bacterial toxin. J Am Chem Soc (2009) 0.99
Oligomerization of DNMT3A controls the mechanism of de novo DNA methylation. J Biol Chem (2011) 0.99
The role of DNA bending in type IIA topoisomerase function. Nucleic Acids Res (2013) 0.98
Multiple forms of Spire-actin complexes and their functional consequences. J Biol Chem (2012) 0.98
Single-molecule analysis reveals three phases of DNA degradation by an exonuclease. Nat Chem Biol (2011) 0.97
Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions. Proc Natl Acad Sci U S A (2004) 0.97
Dynamic look at DNA unwinding by a replicative helicase. Proc Natl Acad Sci U S A (2014) 0.96
The effect of N- or C-terminal alterations of the connector of bacteriophage phi29 DNA packaging motor on procapsid assembly, pRNA binding, and DNA packaging. Nanomedicine (2008) 0.96
Construction of bacteriophage phi29 DNA packaging motor and its applications in nanotechnology and therapy. Ann Biomed Eng (2009) 0.96
A single-molecule view of the assembly pathway, subunit stoichiometry, and unwinding activity of the bacteriophage T4 primosome (helicase-primase) complex. Biochemistry (2013) 0.96
Cylindrical illumination confocal spectroscopy: rectifying the limitations of confocal single molecule spectroscopy through one-dimensional beam shaping. Biophys J (2008) 0.95
A promiscuous DNA packaging machine from bacteriophage T4. PLoS Biol (2011) 0.95
The importance of surfaces in single-molecule bioscience. Mol Biosyst (2008) 0.94
Tyrosine phosphorylation enhances RAD52-mediated annealing by modulating its DNA binding. EMBO J (2011) 0.94
FBH1 helicase disrupts RAD51 filaments in vitro and modulates homologous recombination in mammalian cells. J Biol Chem (2013) 0.94
Branch migration enzyme as a Brownian ratchet. EMBO J (2008) 0.94
Stepwise loading of yeast clamp revealed by ensemble and single-molecule studies. Proc Natl Acad Sci U S A (2010) 0.93
Investigation of stoichiometry of T4 bacteriophage helicase loader protein (gp59). J Biol Chem (2009) 0.93
Visualizing helicases unwinding DNA at the single molecule level. Nucleic Acids Res (2010) 0.93
tRNA dynamics on the ribosome during translation. Proc Natl Acad Sci U S A (2004) 5.38
Correlating structural dynamics and function in single ribozyme molecules. Science (2002) 5.16
tRNA selection and kinetic proofreading in translation. Nat Struct Mol Biol (2004) 4.54
Visualizing infection of individual influenza viruses. Proc Natl Acad Sci U S A (2003) 4.10
Repetitive shuttling of a motor protein on DNA. Nature (2005) 3.82
Probing single-stranded DNA conformational flexibility using fluorescence spectroscopy. Biophys J (2004) 3.69
RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP. Nature (2006) 3.67
Real-time observation of RecA filament dynamics with single monomer resolution. Cell (2006) 3.44
SSB as an organizer/mobilizer of genome maintenance complexes. Crit Rev Biochem Mol Biol (2008) 3.19
Accessory proteins stabilize the acceptor complex for synaptobrevin, the 1:1 syntaxin/SNAP-25 complex. Structure (2008) 3.01
Mechanism of ATP-dependent translocation of E.coli UvrD monomers along single-stranded DNA. J Mol Biol (2004) 2.86
Exploring the folding landscape of a structured RNA. Proc Natl Acad Sci U S A (2001) 2.78
Single molecule observation of liposome-bilayer fusion thermally induced by soluble N-ethyl maleimide sensitive-factor attachment protein receptors (SNAREs). Biophys J (2004) 2.75
One at a time, live tracking of NGF axonal transport using quantum dots. Proc Natl Acad Sci U S A (2007) 2.74
Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton. Nat Methods (2012) 2.67
Clonality of mouse and human cardiomyogenesis in vivo. Proc Natl Acad Sci U S A (2009) 2.60
Mg2+-dependent conformational change of RNA studied by fluorescence correlation and FRET on immobilized single molecules. Proc Natl Acad Sci U S A (2002) 2.47
Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes. Proc Natl Acad Sci U S A (2012) 2.46
DNA-binding orientation and domain conformation of the E. coli rep helicase monomer bound to a partial duplex junction: single-molecule studies of fluorescently labeled enzymes. J Mol Biol (2004) 2.40
Molecular architecture and assembly principles of Vibrio cholerae biofilms. Science (2012) 2.36
SSB protein diffusion on single-stranded DNA stimulates RecA filament formation. Nature (2009) 2.33
Single-molecule transition-state analysis of RNA folding. Proc Natl Acad Sci U S A (2003) 2.31
In vitro system capable of differentiating fast Ca2+-triggered content mixing from lipid exchange for mechanistic studies of neurotransmitter release. Proc Natl Acad Sci U S A (2011) 2.27
A Dimer of Escherichia coli UvrD is the active form of the helicase in vitro. J Mol Biol (2003) 2.26
PcrA helicase dismantles RecA filaments by reeling in DNA in uniform steps. Cell (2010) 2.10
Single-molecule FRET-derived model of the synaptotagmin 1-SNARE fusion complex. Nat Struct Mol Biol (2010) 2.10
Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion. Elife (2012) 2.04
Structural insight into brassinosteroid perception by BRI1. Nature (2011) 2.04
Crystal structure of the FTO protein reveals basis for its substrate specificity. Nature (2010) 2.04
Micromixing within microfluidic devices. Top Curr Chem (2011) 2.03
A nonuniform stepping mechanism for E. coli UvrD monomer translocation along single-stranded DNA. Mol Cell (2007) 2.00
Subnanometre single-molecule localization, registration and distance measurements. Nature (2010) 1.98
Site-specific labeling of the ribosome for single-molecule spectroscopy. Nucleic Acids Res (2005) 1.97
Autoinhibition of Escherichia coli Rep monomer helicase activity by its 2B subdomain. Proc Natl Acad Sci U S A (2005) 1.95
Single-molecule studies of SNARE complex assembly reveal parallel and antiparallel configurations. Proc Natl Acad Sci U S A (2003) 1.94
Characterizing the initial encounter complex in cadherin adhesion. Structure (2009) 1.90
Fluctuations of transfer RNAs between classical and hybrid states. Biophys J (2007) 1.82
Resolving cadherin interactions and binding cooperativity at the single-molecule level. Proc Natl Acad Sci U S A (2008) 1.81
Role of laparoscopy in treatment of choledochal cysts in children. Pediatr Surg Int (2013) 1.78
Revisiting the central dogma one molecule at a time. Cell (2011) 1.75
Srs2 disassembles Rad51 filaments by a protein-protein interaction triggering ATP turnover and dissociation of Rad51 from DNA. Mol Cell (2009) 1.73
Bacillus stearothermophilus PcrA monomer is a single-stranded DNA translocase but not a processive helicase in vitro. J Biol Chem (2007) 1.71
General methods for analysis of sequential "n-step" kinetic mechanisms: application to single turnover kinetics of helicase-catalyzed DNA unwinding. Biophys J (2003) 1.70
Multiple native states reveal persistent ruggedness of an RNA folding landscape. Nature (2010) 1.69
The role of fluctuations in tRNA selection by the ribosome. Proc Natl Acad Sci U S A (2007) 1.69
DNA methylation increases nucleosome compaction and rigidity. J Am Chem Soc (2010) 1.67
Exploration of the transition state for tertiary structure formation between an RNA helix and a large structured RNA. J Mol Biol (2003) 1.67
Dynamic structural rearrangements between DNA binding modes of E. coli SSB protein. J Mol Biol (2007) 1.65
A technique for retrograde intubation in mice. Lab Anim (NY) (2006) 1.65
Coiled-coil domain-dependent homodimerization of intracellular barley immune receptors defines a minimal functional module for triggering cell death. Cell Host Microbe (2011) 1.65
Molecular mechanism of the synaptotagmin-SNARE interaction in Ca2+-triggered vesicle fusion. Nat Struct Mol Biol (2010) 1.64
Dissecting metal ion-dependent folding and catalysis of a single DNAzyme. Nat Chem Biol (2007) 1.63
Single-molecule studies of synaptotagmin and complexin binding to the SNARE complex. Biophys J (2005) 1.60
Single-molecule studies of the neuronal SNARE fusion machinery. Annu Rev Biochem (2009) 1.55
Peptide bond formation destabilizes Shine-Dalgarno interaction on the ribosome. Nature (2007) 1.53
SSB functions as a sliding platform that migrates on DNA via reptation. Cell (2011) 1.53
A precision measurement of the gravitational redshift by the interference of matter waves. Nature (2010) 1.52
Resveratrol facilitates pain attenuation in a rat model of neuropathic pain through the activation of spinal Sirt1. Reg Anesth Pain Med (2013) 1.50
Triage of pediatric injuries after the 2008 Wen-Chuan earthquake in China. J Pediatr Surg (2009) 1.50
Stopped-flow studies of the kinetics of single-stranded DNA binding and wrapping around the Escherichia coli SSB tetramer. Biochemistry (2002) 1.49
9-Butyl-9H-carbazole. Acta Crystallogr Sect E Struct Rep Online (2009) 1.47
GFP-based FRET analysis in live cells. Brain Res (2006) 1.46
ATP-dependent translocation of proteins along single-stranded DNA: models and methods of analysis of pre-steady state kinetics. J Mol Biol (2004) 1.45
DNA unwinding step-size of E. coli RecBCD helicase determined from single turnover chemical quenched-flow kinetic studies. J Mol Biol (2002) 1.44
Growth hormone improves growth in pediatric renal transplant recipients--a systemic review and meta-analysis of randomized controlled trials. Pediatr Nephrol (2012) 1.43
Observation of polymer conformation hysteresis in extensional flow. Science (2003) 1.39
Single-molecule views of protein movement on single-stranded DNA. Annu Rev Biophys (2012) 1.39
Carbon capture and sequestration. Science (2009) 1.39
Regulation of plant innate immunity by three proteins in a complex conserved across the plant and animal kingdoms. Genes Dev (2007) 1.38
Accuracy of inter-hospital paediatric appendicitis referrals. Emerg Med Australas (2012) 1.38
The 2B domain of the Escherichia coli Rep protein is not required for DNA helicase activity. Proc Natl Acad Sci U S A (2002) 1.37
Applications of science and engineering to quantify and control the Deepwater Horizon oil spill. Proc Natl Acad Sci U S A (2012) 1.35
MicroRNA-223 regulates FOXO1 expression and cell proliferation. FEBS Lett (2012) 1.35
Microsecond dynamics of protein-DNA interactions: direct observation of the wrapping/unwrapping kinetics of single-stranded DNA around the E. coli SSB tetramer. J Mol Biol (2006) 1.32
The C-terminal domain of full-length E. coli SSB is disordered even when bound to DNA. Protein Sci (2004) 1.31
Gene targeting of GAN in mouse causes a toxic accumulation of microtubule-associated protein 8 and impaired retrograde axonal transport. Hum Mol Genet (2006) 1.30
Saccharomyces cerevisiae replication protein A binds to single-stranded DNA in multiple salt-dependent modes. Biochemistry (2006) 1.27
Single-molecule superresolution imaging allows quantitative analysis of RAF multimer formation and signaling. Proc Natl Acad Sci U S A (2013) 1.27
Effects of temperature and ATP on the kinetic mechanism and kinetic step-size for E.coli RecBCD helicase-catalyzed DNA unwinding. J Mol Biol (2004) 1.27
Transcription initiation by human RNA polymerase II visualized at single-molecule resolution. Genes Dev (2012) 1.24
Regulation of single-stranded DNA binding by the C termini of Escherichia coli single-stranded DNA-binding (SSB) protein. J Biol Chem (2010) 1.22
Sinonasal undifferentiated carcinoma and nasopharyngeal-type undifferentiated carcinoma: two clinically, biologically, and histopathologically distinct entities. Am J Surg Pathol (2002) 1.22
Kinetic mechanism of direct transfer of Escherichia coli SSB tetramers between single-stranded DNA molecules. Biochemistry (2002) 1.22
Science in support of the Deepwater Horizon response. Proc Natl Acad Sci U S A (2012) 1.21
Conformational trapping of mismatch recognition complex MSH2/MSH3 on repair-resistant DNA loops. Proc Natl Acad Sci U S A (2011) 1.21
Three-dimensional molecular modeling with single molecule FRET. J Struct Biol (2010) 1.20
5'-Single-stranded/duplex DNA junctions are loading sites for E. coli UvrD translocase. EMBO J (2010) 1.16
Ultrahigh-resolution imaging reveals formation of neuronal SNARE/Munc18 complexes in situ. Proc Natl Acad Sci U S A (2013) 1.16
Fluorescence stopped-flow studies of single turnover kinetics of E.coli RecBCD helicase-catalyzed DNA unwinding. J Mol Biol (2004) 1.16
Laparoscopic versus Open Roux-en-Y hepatojejunostomy for children with choledochal cysts: intermediate-term follow-up results. Surg Endosc (2010) 1.15
Direct measurement of tertiary contact cooperativity in RNA folding. J Am Chem Soc (2008) 1.15