Published in Microb Cell Fact on March 07, 2008
BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng (2011) 1.79
High-copy bacterial plasmids diffuse in the nucleoid-free space, replicate stochastically and are randomly partitioned at cell division. Nucleic Acids Res (2013) 1.04
Taking phage integration to the next level as a genetic tool for mycobacteria. Gene (2010) 1.04
The seed composition of Arabidopsis mutants for the group 3 sulfate transporters indicates a role in sulfate translocation within developing seeds. Plant Physiol (2010) 1.01
Dynamic modulation of DNA replication and gene transcription in deep-sea filamentous phage SW1 in response to changes of host growth and temperature. PLoS One (2012) 1.01
Determination of plasmid copy number reveals the total plasmid DNA amount is greater than the chromosomal DNA amount in Bacillus thuringiensis YBT-1520. PLoS One (2011) 0.99
The purine-utilizing bacterium Clostridium acidurici 9a: a genome-guided metabolic reconsideration. PLoS One (2012) 0.95
Combined effect of improved cell yield and increased specific productivity enhances recombinant enzyme production in genome-reduced Bacillus subtilis strain MGB874. Appl Environ Microbiol (2011) 0.88
Acquisition through horizontal gene transfer of plasmid pSMA198 by Streptococcus macedonicus ACA-DC 198 points towards the dairy origin of the species. PLoS One (2015) 0.86
Microarray and functional analysis of growth phase-dependent gene regulation in Bordetella bronchiseptica. Infect Immun (2009) 0.83
Conjugative Type 4 secretion system of a novel large plasmid from the chemoautotroph Tetrathiobacter kashmirensis and construction of shuttle vectors for Alcaligenaceae. Appl Environ Microbiol (2009) 0.82
Heterologous expression of a recombinant lactobacillal β-galactosidase in Lactobacillus plantarum: effect of different parameters on the sakacin P-based expression system. Microb Cell Fact (2015) 0.81
Development of an efficient in vivo system (Pjunc-TpaseIS1223) for random transposon mutagenesis of Lactobacillus casei. Appl Environ Microbiol (2012) 0.79
Rampant Parasexuality Evolves in a Hospital Pathogen during Antibiotic Selection. Mol Biol Evol (2015) 0.79
Quantitative Localization Microscopy Reveals a Novel Organization of a High-Copy Number Plasmid. Biophys J (2016) 0.78
Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum. Microb Cell Fact (2016) 0.77
A model for the evolution of biological specificity: a cross-reacting DNA-binding protein causes plasmid incompatibility. J Bacteriol (2014) 0.76
Programmed evolution for optimization of orthogonal metabolic output in bacteria. PLoS One (2015) 0.76
ZpdN, a Plasmid-Encoded Sigma Factor Homolog, Induces pBS32-Dependent Cell Death in Bacillus subtilis. J Bacteriol (2016) 0.75
The scientific impact of microbial cell factories. Microb Cell Fact (2008) 0.75
Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR. Microb Cell Fact (2016) 0.75
Comparison of six commercial kits to extract bacterial chromosome and plasmid DNA for MiSeq sequencing. Sci Rep (2016) 0.75
Plasmid Copy Number Determination by Quantitative Polymerase Chain Reaction. Sci Pharm (2016) 0.75
A synthetic biology standard for Chinese Hamster Ovary cell genome monitoring and contaminant detection by polymerase chain reaction. Springerplus (2016) 0.75
Generation of a stable plasmid for in vitro and in vivo studies of Staphylococcus. Appl Environ Microbiol (2016) 0.75
Evaluation of CTX-M steady-state mRNA, mRNA half-life and protein production in various STs of Escherichia coli. J Antimicrob Chemother (2015) 0.75
Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR. PLoS One (2017) 0.75
Expression of Lactate Dehydrogenase in Aspergillus niger for L-Lactic Acid Production. PLoS One (2015) 0.75
Characterization of plasmid pML21 of Enterococcus faecalis ML21 from koumiss. Curr Microbiol (2012) 0.75
Synchronized DNA cycling across a bacterial population. Nat Genet (2017) 0.75
A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res (2001) 124.61
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Real-time RT-PCR normalisation; strategies and considerations. Genes Immun (2005) 10.93
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Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli. J Biotechnol (2006) 2.78
The replication-related organization of bacterial genomes. Microbiology (2004) 2.69
Quantitative real-time PCR assay for determining transgene copy number in transformed plants. Biotechniques (2001) 2.01
Detection and traceability of genetically modified organisms in the food production chain. Food Chem Toxicol (2004) 1.92
Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals. Biotechniques (2004) 1.80
PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal Bioanal Chem (2003) 1.77
Estimating the number of integrations in transformed plants by quantitative real-time PCR. BMC Biotechnol (2002) 1.77
Plasmid copy number and plasmid stability. Adv Biochem Eng Biotechnol (2004) 1.62
Use of real-time PCR for determining copy number and zygosity in transgenic plants. Plant Cell Rep (2004) 1.61
Detection and monitoring of virus infections by real-time PCR. Mol Aspects Med (2006) 1.54
Quantitative real-time polymerase chain reaction for determination of plasmid copy number in bacteria. J Microbiol Methods (2005) 1.44
The silent P mating type locus in fission yeast contains two autonomously replicating sequences. Nucleic Acids Res (1993) 1.16
Rapid detection of diarrheagenic E. coli by real-time PCR. J Microbiol Methods (2005) 1.12
The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative real-time PCR. J Microbiol Methods (2005) 1.10
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Directed evolution of copy number of a broad host range plasmid for metabolic engineering. Metab Eng (2005) 1.00
Time-course determination of plasmid content in eukaryotic and prokaryotic cells using real-time PCR. Mol Biotechnol (2007) 1.00
Impact of targeted vector design on Co/E1 plasmid replication. Trends Biotechnol (2002) 0.94
An improved method for determination of gene copy numbers in transgenic mice by serial dilution curves obtained by real-time quantitative PCR assay. J Biochem Biophys Methods (2005) 0.89
Comparison of culture, PCR and immunoassays for detecting Escherichia coli O157 following enrichment culture and immunomagnetic separation performed on naturally contaminated raw meat products. Int J Food Microbiol (2001) 0.89
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Rapid determination of plasmid copy number. J Biotechnol (1996) 0.83
A quantitative real-time PCR assay for the detection of tetR of Tn10 in Escherichia coli using SYBR Green and the Opticon. J Biochem Biophys Methods (2004) 0.81
Purification of streptomycin adenylyltransferase from a recombinant Escherichia coli. Protein Expr Purif (2005) 0.80
Determination of protein expression and plasmid copy number from cloned genes in Escherichia coli by flow injection analysis using an enzyme indicator vector. Biotechnol Bioeng (1989) 0.78
Production of nonclassical inclusion bodies from which correctly folded protein can be extracted. Biotechnol Prog (2005) 1.46
Phage display: selecting straws instead of a needle from a haystack. Molecules (2011) 1.30
Peptide phage display as a tool for drug discovery: targeting membrane receptors. Molecules (2011) 1.28
Obstacles and pitfalls in the PEGylation of therapeutic proteins. Curr Opin Drug Discov Devel (2008) 1.27
Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells. J Ind Microbiol Biotechnol (2013) 1.13
Protective effects of fullerenol C60(OH)24 against doxorubicin-induced cardiotoxicity and hepatotoxicity in rats with colorectal cancer. Biomaterials (2008) 1.09
Acute doxorubicin nephrotoxicity in rats with malignant neoplasm can be successfully treated with fullerenol C60(OH)24 via suppression of oxidative stress. Pharmacol Rep (2008) 1.03
Molecular basis of Colorado potato beetle adaptation to potato plant defence at the level of digestive cysteine proteinases. Insect Biochem Mol Biol (2004) 0.99
New properties of inclusion bodies with implications for biotechnology. Biotechnol Appl Biochem (2008) 0.97
Potential hepatoprotective effects of fullerenol C60(OH)24 in doxorubicin-induced hepatotoxicity in rats with mammary carcinomas. Biomaterials (2008) 0.93
Influence of the protein oligomericity on final yield after affinity tag removal in purification of recombinant proteins. J Chromatogr A (2005) 0.93
High expression of green fluorescent protein in Pichia pastoris leads to formation of fluorescent particles. J Biotechnol (2004) 0.89
Expression of sea anemone equistatin in potato. Effects of plant proteases on heterologous protein production. Plant Physiol (2003) 0.89
Purification, characterization and cloning of a ricin B-like lectin from mushroom Clitocybe nebularis with antiproliferative activity against human leukemic T cells. Biochim Biophys Acta (2008) 0.89
Constitutive versus thermoinducible expression of heterologous proteins in Escherichia coli based on strong PR,PL promoters from phage lambda. Biotechnol Bioeng (2003) 0.87
Identification of the heparin-binding domain of TNF-alpha and its use for efficient TNF-alpha purification by heparin-Sepharose affinity chromatography. J Chromatogr B Analyt Technol Biomed Life Sci (2008) 0.87
Endophytic fungi: the treasure chest of antibacterial substances. Phytomedicine (2012) 0.87
Engineered lactic acid bacterium Lactococcus lactis capable of binding antibodies and tumor necrosis factor alpha. Appl Environ Microbiol (2010) 0.85
Acute doxorubicin pulmotoxicity in rats with malignant neoplasm is effectively treated with fullerenol C60(OH)24 through inhibition of oxidative stress. Pharmacol Rep (2009) 0.85
Screening of selected food and medicinal plant extracts for pancreatic lipase inhibition. Phytother Res (2009) 0.85
Effects of high-fat mixed-lipid diet and exercise on the antioxidant system in skeletal and cardiac muscles of rats with colon carcinoma. Pharmacol Rep (2009) 0.84
Attachment of histidine tags to recombinant tumor necrosis factor-alpha drastically changes its properties. ScientificWorldJournal (2002) 0.83
Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis: a path toward sweet lactic acid bacteria. Appl Microbiol Biotechnol (2006) 0.83
Comparison of bacterial and phage display peptide libraries in search of target-binding motif. Appl Biochem Biotechnol (2005) 0.82
Fullerenol nanoparticles: toxicity and antioxidant activity. Methods Mol Biol (2013) 0.81
Heterogeneity in the cysteine protease inhibitor clitocypin gene family. Biol Chem (2006) 0.81
Exploiting microRNAs for cell engineering and therapy. Biotechnol Adv (2012) 0.81
Basidiomycete Clitocybe nebularis is rich in lectins with insecticidal activities. Appl Microbiol Biotechnol (2011) 0.81
Rutin in buckwheat herbs grown at different UV-B radiation levels: comparison of two UV spectrophotometric and an HPLC method. J Exp Bot (2002) 0.81
CNL, a ricin B-like lectin from mushroom Clitocybe nebularis, induces maturation and activation of dendritic cells via the toll-like receptor 4 pathway. Immunology (2011) 0.81
Diverse enzymatic specificities of digestive proteases, 'intestains', enable Colorado potato beetle larvae to counteract the potato defence mechanism. Biol Chem (2003) 0.81
Engineered staphylococcal protein A's IgG-binding domain with cathepsin L inhibitory activity. Biochem Biophys Res Commun (2006) 0.81
Activity of antioxidative enzymes in erythrocytes after a single dose administration of doxorubicin in rats pretreated with fullerenol C(60)(OH)(24). Toxicol Mech Methods (2009) 0.80
Calcipotriol affects keratinocyte proliferation by decreasing expression of early growth response-1 and polo-like kinase-2. Pharm Res (2007) 0.80
Ultrasound in phage display: a new approach to nonspecific elution. Biotechniques (2008) 0.80
Determination of doxycycline in pharmaceuticals and human urine by micellar electrokinetic capillary chromatography. Anal Bioanal Chem (2006) 0.79
Biosimilars, generic versions of the first generation of therapeutic proteins: do they exist? Contrib Nephrol (2005) 0.78
Gene Expression Profiling of Recombinant Protein Producing E. coli at Suboptimal Growth Temperature. Acta Chim Slov (2012) 0.78
Novel applications of recombinant lactic acid bacteria in therapy and in metabolic engineering. Recent Pat Biotechnol (2009) 0.78
Improvement of potential therapeutic value of tumor necrosis-alpha (TNF-alpha) by charge modulation in the tip region. Eur Cytokine Netw (2005) 0.78
Thermal stability of the WHO international standard of interferon alpha 2b (IFN-alpha 2b): application of new reporter gene assay for IFN-alpha 2b potency determinations. J Immunol Methods (2006) 0.77
Ibogaine affects brain energy metabolism. Eur J Pharmacol (2006) 0.77
Affinity selection to papain yields potent peptide inhibitors of cathepsins L, B, H, and K. Biochem Biophys Res Commun (2005) 0.77
HWGMWSY, an unanticipated polystyrene binding peptide from random phage display libraries. Anal Biochem (2012) 0.77
SPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: comparison of direct injection to SPE-MEKC. Electrophoresis (2008) 0.77
Peptide inhibitors of MurD and MurE, essential enzymes of bacterial cell wall biosynthesis. J Basic Microbiol (2008) 0.77
Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography. Food Addit Contam Part A Chem Anal Control Expo Risk Assess (2008) 0.76
Correlations between in vitro potency of polyethylene glycol-protein conjugates and their chromatographic behavior. Anal Biochem (2009) 0.76
Improved acylation method enables efficient delivery of functional palmitoylated cystatin into epithelial cells. Chem Biol Drug Des (2007) 0.76
Screening of wood damaging fungi and macrofungi for inhibitors of pancreatic lipase. Phytother Res (2004) 0.75
Screening for antibacterial activity in 72 species of wood-colonizing fungi by the Vibrio fisheri bioluminescence method. J Basic Microbiol (2004) 0.75
Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography. J Chromatogr Sci (2008) 0.75
Metabolic plasticity and the energy economizing effect of ibogaine, the principal alkaloid of Tabernanthe iboga. J Ethnopharmacol (2012) 0.75
Induction of energy metabolism related enzymes in yeast Saccharomyces cerevisiae exposed to ibogaine is adaptation to acute decrease in ATP energy pool. Eur J Pharmacol (2009) 0.75
New assay for quantification of PEGylated proteins during in vitro permeability studies. Acta Chim Slov (2014) 0.75
Chemoporation using saponins or cholates: an alternative method for transformation of bacterial cells. Biotechnol Lett (2009) 0.75