Published in Microb Cell Fact on December 01, 2008
Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microb Cell Fact (2005) 2.67
Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. Microb Cell Fact (2008) 1.89
Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins. Microb Cell Fact (2005) 1.79
Comparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinant Saccharomyces cerevisiae. Microb Cell Fact (2007) 1.74
Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins. Microb Cell Fact (2004) 1.64
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors. Microb Cell Fact (2005) 1.59
Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review. Microb Cell Fact (2006) 1.49
Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis. Microb Cell Fact (2005) 1.47
Protein secretion in Lactococcus lactis : an efficient way to increase the overall heterologous protein production. Microb Cell Fact (2005) 1.45
Rhizobial exopolysaccharides: genetic control and symbiotic functions. Microb Cell Fact (2006) 1.41
Regulation of methanol utilisation pathway genes in yeasts. Microb Cell Fact (2006) 1.39
Role of cultivation media in the development of yeast strains for large scale industrial use. Microb Cell Fact (2005) 1.38
Improvement of Escherichia coli production strains by modification of the phosphoenolpyruvate:sugar phosphotransferase system. Microb Cell Fact (2005) 1.35
Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes. Microb Cell Fact (2008) 1.32
Fluxome analysis using GC-MS. Microb Cell Fact (2007) 1.31
Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae. Microb Cell Fact (2008) 1.30
Miniature bioreactors: current practices and future opportunities. Microb Cell Fact (2006) 1.30
Hydrogen production by Cyanobacteria. Microb Cell Fact (2005) 1.29
Unscrambling an egg: protein disaggregation by AAA+ proteins. Microb Cell Fact (2004) 1.19
Protein quality control in the bacterial periplasm. Microb Cell Fact (2004) 1.19
Transient increase of ATP as a response to temperature up-shift in Escherichia coli. Microb Cell Fact (2005) 1.18
Applications of cell sorting in biotechnology. Microb Cell Fact (2006) 1.17
Biosynthesis of isoprenoids, polyunsaturated fatty acids and flavonoids in Saccharomyces cerevisiae. Microb Cell Fact (2006) 1.17
Thioredoxin reductase is a key factor in the oxidative stress response of Lactobacillus plantarum WCFS1. Microb Cell Fact (2007) 1.17
Spider silks: recombinant synthesis, assembly, spinning, and engineering of synthetic proteins. Microb Cell Fact (2004) 1.16
Regulation of xylose metabolism in recombinant Saccharomyces cerevisiae. Microb Cell Fact (2008) 1.15
Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones. Microb Cell Fact (2005) 1.15
Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum. Microb Cell Fact (2007) 1.14
Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation. Microb Cell Fact (2004) 1.13
Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism. Microb Cell Fact (2006) 1.12
Industrial-scale production and purification of a heterologous protein in Lactococcus lactis using the nisin-controlled gene expression system NICE: the case of lysostaphin. Microb Cell Fact (2005) 1.12
Optimization of the Lactococcus lactis nisin-controlled gene expression system NICE for industrial applications. Microb Cell Fact (2005) 1.11
Sequence determinants of protein aggregation: tools to increase protein solubility. Microb Cell Fact (2005) 1.09
Vanillin production using metabolically engineered Escherichia coli under non-growing conditions. Microb Cell Fact (2007) 1.08
Decreased gene expression from T7 promoters may be due to impaired production of active T7 RNA polymerase. Microb Cell Fact (2005) 1.07
The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. Microb Cell Fact (2003) 1.07
Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red(R)/ET(R) Recombination. Microb Cell Fact (2008) 1.06
A genomic search approach to identify esterases in Propionibacterium freudenreichii involved in the formation of flavour in Emmental cheese. Microb Cell Fact (2008) 1.05
A perspective on microarrays: current applications, pitfalls, and potential uses. Microb Cell Fact (2007) 1.05
Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes. Microb Cell Fact (2008) 1.05
Minimal information: an urgent need to assess the functional reliability of recombinant proteins used in biological experiments. Microb Cell Fact (2008) 1.04
Conversion of beta-carotene into astaxanthin: Two separate enzymes or a bifunctional hydroxylase-ketolase protein? Microb Cell Fact (2008) 1.03
The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies. Microb Cell Fact (2005) 1.02
Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates. Microb Cell Fact (2003) 1.02
Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine. Microb Cell Fact (2007) 1.01
Ensuring safety of DNA vaccines. Microb Cell Fact (2005) 1.00
Molecular and bioengineering strategies to improve alginate and polydydroxyalkanoate production by Azotobacter vinelandii. Microb Cell Fact (2007) 0.99
Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris. Microb Cell Fact (2008) 0.99
Actinomycetes as host cells for production of recombinant proteins. Microb Cell Fact (2005) 0.99
Yeast cell factories for fine chemical and API production. Microb Cell Fact (2008) 0.99
Metabolic responses to pyruvate kinase deletion in lysine producing Corynebacterium glutamicum. Microb Cell Fact (2008) 0.98
Coutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system. Microb Cell Fact (2008) 0.96
Improvement of bioprocess monitoring: development of novel concepts. Microb Cell Fact (2006) 0.96
Lactate production yield from engineered yeasts is dependent from the host background, the lactate dehydrogenase source and the lactate export. Microb Cell Fact (2006) 0.95
Complete gene expression profiling of Saccharopolyspora erythraea using GeneChip DNA microarrays. Microb Cell Fact (2007) 0.94
Optimisation of batch culture conditions for cyclodextrin glucanotransferase production from Bacillus circulans DF 9R. Microb Cell Fact (2002) 0.93
Screening for novel enzymes from metagenome and SIGEX, as a way to improve it. Microb Cell Fact (2005) 0.93
Bacterial autoinduction: looking outside the cell for new metabolic engineering targets. Microb Cell Fact (2002) 0.93
Purification and partial characterization of bacillocin 490, a novel bacteriocin produced by a thermophilic strain of Bacillus licheniformis. Microb Cell Fact (2002) 0.92
Stress responses and replication of plasmids in bacterial cells. Microb Cell Fact (2002) 0.90
Directed evolution of single-chain Fv for cytoplasmic expression using the beta-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation. Microb Cell Fact (2004) 0.89
Bioinformatics in microbial biotechnology--a mini review. Microb Cell Fact (2005) 0.88
Cheese whey-induced high-cell-density production of recombinant proteins in Escherichia coli. Microb Cell Fact (2003) 0.88
Microbial proteomics: a mass spectrometry primer for biologists. Microb Cell Fact (2007) 0.88
A step ahead: combining protein purification and correct folding selection. Microb Cell Fact (2004) 0.87
Metabolic engineering of microbes for oligosaccharide and polysaccharide synthesis. Microb Cell Fact (2006) 0.87
Identification of pathogenic microbial cells and spores by electrochemical detection on a biochip. Microb Cell Fact (2004) 0.86
Altering coenzyme specificity of Pichia stipitis xylose reductase by the semi-rational approach CASTing. Microb Cell Fact (2007) 0.86
Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger beta-galactosidase. Microb Cell Fact (2006) 0.86
Development of an improved Pseudoalteromonas haloplanktis TAC125 strain for recombinant protein secretion at low temperature. Microb Cell Fact (2008) 0.86
Monitoring protein stability in vivo. Microb Cell Fact (2005) 0.86
Nisin inducible production of listeriolysin O in Lactococcus lactis NZ9000. Microb Cell Fact (2008) 0.86
Use of pIVEX plasmids for protein overproduction in Escherichia coli. Microb Cell Fact (2005) 0.86
Characterization of the iron-regulated desA promoter of Streptomyces pilosus as a system for controlled gene expression in actinomycetes. Microb Cell Fact (2003) 0.85
A general method for selection of riboflavin-overproducing food grade micro-organisms. Microb Cell Fact (2006) 0.85
Developing nucleic acid-based electrical detection systems. Microb Cell Fact (2006) 0.85
Biological treatment of tannery wastewater by using salt-tolerant bacterial strains. Microb Cell Fact (2008) 0.83
The role of bacterial antizyme: From an inhibitory protein to AtoC transcriptional regulator. Microb Cell Fact (2004) 0.82
Comparative modelling of protein structure and its impact on microbial cell factories. Microb Cell Fact (2005) 0.82
Inteins and affinity resin substitutes for protein purification and scale up. Microb Cell Fact (2005) 0.82
CcpA affects expression of the groESL and dnaK operons in Lactobacillus plantarum. Microb Cell Fact (2006) 0.81
Expression of Brugmansia candida Hyoscyamine 6beta-Hydroxylase gene in Saccharomyces cerevisiae and its potential use as biocatalyst. Microb Cell Fact (2008) 0.79
Switching the mode of sucrose utilization by Saccharomyces cerevisiae. Microb Cell Fact (2008) 0.79
Encapsulated Escherichia coli in alginate beads capable of secreting a heterologous pectin lyase. Microb Cell Fact (2005) 0.79
Determination of bacteriocin activity with bioassays carried out on solid and liquid substrates: assessing the factor "indicator microorganism". Microb Cell Fact (2006) 0.78
The bag or the spindle: the cell factory at the time of systems' biology. Microb Cell Fact (2004) 0.77
Redox stress proteins are involved in adaptation response of the hyperthermoacidophilic archaeon Sulfolobus solfataricus to nickel challenge. Microb Cell Fact (2007) 0.77
Keeping up with protein folding. Microb Cell Fact (2004) 0.77
Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells. Microb Cell Fact (2005) 0.77
"Intelligent" descriptions of microbial kinetics in finitely dispersed bioreactors: neural and cybernetic models for PHB biosynthesis by Ralstonia eutropha. Microb Cell Fact (2007) 0.76
Systems biology and biological systems diversity for the engineering of microbial cell factories. Microb Cell Fact (2007) 0.76
Microbial production of organic acids: expanding the markets. Trends Biotechnol (2008) 2.88
Protein quality in bacterial inclusion bodies. Trends Biotechnol (2006) 2.02
Amyloid-like properties of bacterial inclusion bodies. J Mol Biol (2005) 1.90
Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. Microb Cell Fact (2008) 1.89
Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins. Microb Cell Fact (2005) 1.79
Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnol Bioeng (2004) 1.64
Microbial factories for recombinant pharmaceuticals. Microb Cell Fact (2009) 1.59
Engineering of Pichia pastoris for improved production of antibody fragments. Biotechnol Bioeng (2006) 1.58
The effect of temperature on the proteome of recombinant Pichia pastoris. J Proteome Res (2009) 1.53
Genistein improves neuropathology and corrects behaviour in a mouse model of neurodegenerative metabolic disease. PLoS One (2010) 1.42
Genistein-mediated inhibition of glycosaminoglycan synthesis as a basis for gene expression-targeted isoflavone therapy for mucopolysaccharidoses. Eur J Hum Genet (2006) 1.41
Recombinant protein production in yeasts. Mol Biotechnol (2005) 1.38
Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays. BMC Genomics (2008) 1.36
The conformational quality of insoluble recombinant proteins is enhanced at low growth temperatures. Biotechnol Bioeng (2007) 1.34
Staphylococcus aureus as an infectious agent: overview of biochemistry and molecular genetics of its pathogenicity. Acta Biochim Pol (2009) 1.31
Adaptive laboratory evolution -- principles and applications for biotechnology. Microb Cell Fact (2013) 1.29
A multi-level study of recombinant Pichia pastoris in different oxygen conditions. BMC Syst Biol (2010) 1.28
Genistein reduces lysosomal storage in peripheral tissues of mucopolysaccharide IIIB mice. Mol Genet Metab (2009) 1.26
Recombinant protein solubility - does more mean better? Nat Biotechnol (2007) 1.21
Localization of functional polypeptides in bacterial inclusion bodies. Appl Environ Microbiol (2006) 1.20
Pichia pastoris: protein production host and model organism for biomedical research. Future Microbiol (2013) 1.19
Hypoxic fed-batch cultivation of Pichia pastoris increases specific and volumetric productivity of recombinant proteins. Biotechnol Bioeng (2008) 1.19
Divergent genetic control of protein solubility and conformational quality in Escherichia coli. J Mol Biol (2007) 1.18
Differential efficiency of induction of various lambdoid prophages responsible for production of Shiga toxins in response to different induction agents. Microb Pathog (2009) 1.18
Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris. Microb Cell Fact (2006) 1.18
Role of molecular chaperones in inclusion body formation. FEBS Lett (2003) 1.16
Genistein-mediated inhibition of glycosaminoglycan synthesis, which corrects storage in cells of patients suffering from mucopolysaccharidoses, acts by influencing an epidermal growth factor-dependent pathway. J Biomed Sci (2009) 1.14
Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production. J Biotechnol (2010) 1.13
Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation. Microb Cell Fact (2004) 1.13
Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts. Appl Environ Microbiol (2007) 1.12
Localization of chaperones DnaK and GroEL in bacterial inclusion bodies. J Bacteriol (2005) 1.12
Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism. Microb Cell Fact (2006) 1.12
Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels. Microb Cell Fact (2009) 1.11
Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris. Microb Cell Fact (2013) 1.09
Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus. FEMS Yeast Res (2009) 1.09
Learning about protein solubility from bacterial inclusion bodies. Microb Cell Fact (2009) 1.09
Hydrogen peroxide-mediated induction of the Shiga toxin-converting lambdoid prophage ST2-8624 in Escherichia coli O157:H7. FEMS Immunol Med Microbiol (2009) 1.09
Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions. BMC Genomics (2007) 1.09
Nanotechnology, bionanotechnology and microbial cell factories. Microb Cell Fact (2010) 1.09
A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastoris. PLoS One (2011) 1.08
Biomedical applications of distally controlled magnetic nanoparticles. Trends Biotechnol (2009) 1.08
Intracellular pH distribution in Saccharomyces cerevisiae cell populations, analyzed by flow cytometry. Appl Environ Microbiol (2005) 1.08
Simple method for plating Escherichia coli bacteriophages forming very small plaques or no plaques under standard conditions. Appl Environ Microbiol (2008) 1.07
Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production. Biotechnol J (2010) 1.07
Bacterial inclusion bodies: making gold from waste. Trends Biotechnol (2011) 1.07
A role for bacteriophage T4 rI gene function in the control of phage development during pseudolysogeny and in slowly growing host cells. Res Microbiol (2003) 1.06
Biochemistry of microbial itaconic acid production. Front Microbiol (2013) 1.06
Improvement of lactic acid production in Saccharomyces cerevisiae by cell sorting for high intracellular pH. Appl Environ Microbiol (2006) 1.06
Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics. Biotechnol Adv (2012) 1.05
Isolation of cell-free bacterial inclusion bodies. Microb Cell Fact (2010) 1.05
Experimental evidence for the physiological role of bacterial luciferase in the protection of cells against oxidative stress. Curr Microbiol (2003) 1.05
ppGpp inhibits the activity of Escherichia coli DnaG primase. Plasmid (2009) 1.04
Biosynthesis of vitamin C by yeast leads to increased stress resistance. PLoS One (2007) 1.03
Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line. Biotechnol Prog (2005) 1.03
Growth-rate dependent RNA polyadenylation in Escherichia coli. EMBO Rep (2003) 1.03
Production of recombinant proteins and metabolites in yeasts: when are these systems better than bacterial production systems? Appl Microbiol Biotechnol (2010) 1.02
The response to unfolded protein is involved in osmotolerance of Pichia pastoris. BMC Genomics (2010) 1.02
The secretory pathway: exploring yeast diversity. FEMS Microbiol Rev (2013) 1.01
Biological role of bacterial inclusion bodies: a model for amyloid aggregation. FEBS J (2011) 1.01
Folding of a misfolding-prone beta-galactosidase in absence of DnaK. Biotechnol Bioeng (2005) 1.01
Side effects of chaperone gene co-expression in recombinant protein production. Microb Cell Fact (2010) 1.01
Tunable geometry of bacterial inclusion bodies as substrate materials for tissue engineering. Nanotechnology (2010) 1.00
Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris. Microb Cell Fact (2008) 0.99
Yield, solubility and conformational quality of soluble proteins are not simultaneously favored in recombinant Escherichia coli. Biotechnol Bioeng (2008) 0.99
In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response. Biotechnol J (2014) 0.99
Substrate deprivation therapy: a new hope for patients suffering from neuronopathic forms of inherited lysosomal storage diseases. J Appl Genet (2007) 0.98
Six novel constitutive promoters for metabolic engineering of Aspergillus niger. Appl Microbiol Biotechnol (2012) 0.98
The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis. BMC Genomics (2011) 0.97
Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast. Free Radic Biol Med (2012) 0.96
Stimulation of DNA repair as an evolutionary drive for bacterial luminescence. Luminescence (2003) 0.96
Monitoring intracellular redox conditions in the endoplasmic reticulum of living yeasts. FEMS Microbiol Lett (2010) 0.96
Overexpression of the cgtA (yhbZ, obgE) gene, coding for an essential GTP-binding protein, impairs the regulation of chromosomal functions in Escherichia coli. Curr Microbiol (2002) 0.96
Impairment of glycosaminoglycan synthesis in mucopolysaccharidosis type IIIA cells by using siRNA: a potential therapeutic approach for Sanfilippo disease. Eur J Hum Genet (2009) 0.96
Nanostructured bacterial materials for innovative medicines. Trends Microbiol (2010) 0.95
Expression of genes coding for GerA and GerK spore germination receptors is dependent on the protein phosphatase PrpE. J Bacteriol (2006) 0.95
Transcription from bacteriophage lambda pR promoter is regulated independently and antagonistically by DksA and ppGpp. Nucleic Acids Res (2009) 0.95
Lactate production yield from engineered yeasts is dependent from the host background, the lactate dehydrogenase source and the lactate export. Microb Cell Fact (2006) 0.95
Engineering building blocks for self-assembling protein nanoparticles. Microb Cell Fact (2010) 0.95
Targeting enzymes to the right compartment: metabolic engineering for itaconic acid production by Aspergillus niger. Metab Eng (2013) 0.95
The chaperone DnaK controls the fractioning of functional protein between soluble and insoluble cell fractions in inclusion body-forming cells. Microb Cell Fact (2006) 0.95
Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris. Microb Cell Fact (2011) 0.95
Genome sequence of the ruminal bacterium Megasphaera elsdenii. J Bacteriol (2011) 0.94
Yeast systems biotechnology for the production of heterologous proteins. FEMS Yeast Res (2009) 0.94
The Functional quality of soluble recombinant polypeptides produced in Escherichia coli is defined by a wide conformational spectrum. Appl Environ Microbiol (2008) 0.94
Differential antibacterial activity of genistein arising from global inhibition of DNA, RNA and protein synthesis in some bacterial strains. Arch Microbiol (2005) 0.93
Engineering of protein folding and secretion-strategies to overcome bottlenecks for efficient production of recombinant proteins. Antioxid Redox Signal (2014) 0.93
Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry. J Biotechnol (2003) 0.93
Effective inhibition of lytic development of bacteriophages lambda, P1 and T4 by starvation of their host, Escherichia coli. BMC Biotechnol (2007) 0.92
Correlation between severity of mucopolysaccharidoses and combination of the residual enzyme activity and efficiency of glycosaminoglycan synthesis. Acta Paediatr (2008) 0.92
Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris. Appl Microbiol Biotechnol (2015) 0.91
Bacteriophage contamination: is there a simple method to reduce its deleterious effects in laboratory cultures and biotechnological factories? J Appl Genet (2004) 0.91
Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure. Microb Cell Fact (2011) 0.91
Two-year follow-up of Sanfilippo Disease patients treated with a genistein-rich isoflavone extract: assessment of effects on cognitive functions and general status of patients. Med Sci Monit (2011) 0.91
Antibody production with yeasts and filamentous fungi: on the road to large scale? Biotechnol Lett (2006) 0.91
Membrane-active peptides for non-viral gene therapy: making the safest easier. Trends Biotechnol (2008) 0.91
16 years research on lactic acid production with yeast - ready for the market? Biotechnol Genet Eng Rev (2010) 0.91
Systems metabolic engineering, industrial biotechnology and microbial cell factories. Microb Cell Fact (2012) 0.90