piggyBac can bypass DNA synthesis during cut and paste transposition.

PubWeight™: 2.27‹?› | Rank: Top 2%

🔗 View Article (PMC 2323262)

Published in EMBO J on March 20, 2008

Authors

Rupak Mitra1, Jennifer Fain-Thornton, Nancy L Craig

Author Affiliations

1: Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205-2185, USA.

Articles citing this

piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature (2009) 11.27

Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon. Nat Methods (2009) 4.27

A hyperactive piggyBac transposase for mammalian applications. Proc Natl Acad Sci U S A (2011) 2.68

Harnessing transposons for cancer gene discovery. Nat Rev Cancer (2010) 2.44

The catalytic domain of all eukaryotic cut-and-paste transposase superfamilies. Proc Natl Acad Sci U S A (2011) 1.65

piggyBac transposon/transposase system to generate CD19-specific T cells for the treatment of B-lineage malignancies. Hum Gene Ther (2010) 1.64

PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements in the ciliate Paramecium tetraurelia. Genes Dev (2009) 1.53

The Paramecium germline genome provides a niche for intragenic parasitic DNA: evolutionary dynamics of internal eliminated sequences. PLoS Genet (2012) 1.38

Integrating prokaryotes and eukaryotes: DNA transposases in light of structure. Crit Rev Biochem Mol Biol (2010) 1.35

piggyBac transposase tools for genome engineering. Proc Natl Acad Sci U S A (2013) 1.35

Helper-independent piggyBac plasmids for gene delivery approaches: strategies for avoiding potential genotoxic effects. Proc Natl Acad Sci U S A (2010) 1.25

Transposon-mediated BAC transgenesis in human ES cells. Nucleic Acids Res (2012) 1.21

Hyperactive piggyBac gene transfer in human cells and in vivo. Hum Gene Ther (2011) 1.21

Epigenetics of ciliates. Cold Spring Harb Perspect Biol (2013) 1.14

Gene therapy vectors: the prospects and potentials of the cut-and-paste transposons. Genetica (2009) 1.10

Transposition of the human Hsmar1 transposon: rate-limiting steps and the importance of the flanking TA dinucleotide in second strand cleavage. Nucleic Acids Res (2009) 1.10

Chimeric piggyBac transposases for genomic targeting in human cells. Nucleic Acids Res (2012) 1.09

Testing the palindromic target site model for DNA transposon insertion using the Drosophila melanogaster P-element. Nucleic Acids Res (2008) 1.08

The expanding universe of transposon technologies for gene and cell engineering. Mob DNA (2010) 1.08

The piggyBac transposon displays local and distant reintegration preferences and can cause mutations at noncanonical integration sites. Mol Cell Biol (2013) 1.01

Transcription activator like effector (TALE)-directed piggyBac transposition in human cells. Nucleic Acids Res (2013) 1.01

PiggyBac transposon-mediated, reversible gene transfer in human embryonic stem cells. Stem Cells Dev (2010) 1.00

Mutational analysis of highly conserved aspartate residues essential to the catalytic core of the piggyBac transposase. BMC Mol Biol (2008) 0.99

Seamless genome editing in human pluripotent stem cells using custom endonuclease-based gene targeting and the piggyBac transposon. Nat Protoc (2013) 0.97

"Calling cards" for DNA-binding proteins in mammalian cells. Genetics (2012) 0.97

Tethering of the conserved piggyBac transposase fusion protein CSB-PGBD3 to chromosomal AP-1 proteins regulates expression of nearby genes in humans. PLoS Genet (2012) 0.96

Highly precise and developmentally programmed genome assembly in Paramecium requires ligase IV-dependent end joining. PLoS Genet (2011) 0.96

Base flipping in V(D)J recombination: insights into the mechanism of hairpin formation, the 12/23 rule, and the coordination of double-strand breaks. Mol Cell Biol (2009) 0.96

A domesticated PiggyBac transposase interacts with heterochromatin and catalyzes reproducible DNA elimination in Tetrahymena. PLoS Genet (2013) 0.94

A resurrected mammalian hAT transposable element and a closely related insect element are highly active in human cell culture. Proc Natl Acad Sci U S A (2012) 0.94

A Hyperactive Transposase Promotes Persistent Gene Transfer of a piggyBac DNA Transposon. Mol Ther Nucleic Acids (2012) 0.93

All roads lead to induced pluripotent stem cells: the technologies of iPSC generation. Stem Cells Dev (2014) 0.91

Crosstalk between transposase subunits during cleavage of the mariner transposon. Nucleic Acids Res (2014) 0.90

Functional characterization of piggyBat from the bat Myotis lucifugus unveils an active mammalian DNA transposon. Proc Natl Acad Sci U S A (2012) 0.90

piggyBac-ing models and new therapeutic strategies. Trends Biotechnol (2015) 0.89

DNA transposon-based gene vehicles - scenes from an evolutionary drive. J Biomed Sci (2013) 0.89

Vectorology and factor delivery in induced pluripotent stem cell reprogramming. Stem Cells Dev (2014) 0.88

Suicidal autointegration of sleeping beauty and piggyBac transposons in eukaryotic cells. PLoS Genet (2014) 0.87

Transposon domestication versus mutualism in ciliate genome rearrangements. PLoS Genet (2013) 0.87

A piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe. Nucleic Acids Res (2011) 0.86

The impact of cHS4 insulators on DNA transposon vector mobilization and silencing in retinal pigment epithelium cells. PLoS One (2012) 0.85

Spy: a new group of eukaryotic DNA transposons without target site duplications. Genome Biol Evol (2014) 0.85

Transposon Invasion of the Paramecium Germline Genome Countered by a Domesticated PiggyBac Transposase and the NHEJ Pathway. Int J Evol Biol (2012) 0.85

Genomic DNA transposition induced by human PGBD5. Elife (2015) 0.85

Programmed Genome Rearrangements in the Ciliate Oxytricha. Microbiol Spectr (2014) 0.84

Removal of the bloom syndrome DNA helicase extends the utility of imprecise transposon excision for making null mutations in Drosophila. Genetics (2009) 0.82

A hyperactive transposase of the maize transposable element activator (Ac). Genetics (2012) 0.81

Genome-wide Profiling Reveals Remarkable Parallels Between Insertion Site Selection Properties of the MLV Retrovirus and the piggyBac Transposon in Primary Human CD4(+) T Cells. Mol Ther (2016) 0.80

Analysis of the piggyBac transposase reveals a functional nuclear targeting signal in the 94 c-terminal residues. BMC Mol Biol (2008) 0.80

Characterization of a group of MITEs with unusual features from two coral genomes. PLoS One (2010) 0.80

In and out of the rRNA genes: characterization of Pokey elements in the sequenced Daphnia genome. Mob DNA (2013) 0.80

piggyBac transposons expressing full-length human dystrophin enable genetic correction of dystrophic mesoangioblasts. Nucleic Acids Res (2015) 0.79

What role (if any) does the highly conserved CSB-PGBD3 fusion protein play in Cockayne syndrome? Mech Ageing Dev (2013) 0.79

Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production. BMC Biotechnol (2010) 0.78

Subtraction by addition: domesticated transposases in programmed DNA elimination. Genes Dev (2009) 0.78

Utilization of Site-Specific Recombination in Biopharmaceutical Production. Iran Biomed J (2015) 0.77

Comparative Analysis of piggyBac, CRISPR/Cas9 and TALEN Mediated BAC Transgenesis in the Zygote for the Generation of Humanized SIRPA Rats. Sci Rep (2016) 0.77

Evolution of a transposon in Daphnia hybrid genomes. Mob DNA (2013) 0.77

PiggyBac transposon vectors: the tools of the human gene encoding. Transl Lung Cancer Res (2016) 0.76

Fusion of reverse-oriented Ds termini following abortive transposition in Arabidopsis: implications for the mechanism of Ac/Ds transposition. Plant Cell Rep (2010) 0.76

An efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori. Sci Rep (2015) 0.76

PiggyBac transposon-based polyadenylation-signal trap for genome-wide mutagenesis in mice. Sci Rep (2016) 0.75

IPB7 transposase behavior in Drosophila melanogaster and Aedes aegypti. Insect Biochem Mol Biol (2013) 0.75

An assay to monitor the activity of DNA transposition complexes yields a general quality control measure for transpositional recombination reactions. Mob Genet Elements (2014) 0.75

A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells. PLoS One (2014) 0.75

A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. J Biol Chem (2017) 0.75

Molecular evolution of piggyBac superfamily: from selfishness to domestication. Genome Biol Evol (2017) 0.75

Endogenous Transposase Source in Human Cells Mobilizes piggyBac Transposons. Mol Ther (2016) 0.75

PGBD5 promotes site-specific oncogenic mutations in human tumors. Nat Genet (2017) 0.75

Profile of Nancy L. Craig. Proc Natl Acad Sci U S A (2012) 0.75

Functional characterization of the active Mutator-like transposable element, Muta1 from the mosquito Aedes aegypti. Mob DNA (2017) 0.75

Multimerization properties of PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements. Nucleic Acids Res (2017) 0.75

Preclinical and clinical advances in transposon-based gene therapy. Biosci Rep (2017) 0.75

Transposable Element Domestication As an Adaptation to Evolutionary Conflicts. Trends Genet (2017) 0.75

Articles cited by this

Protein secondary structure prediction based on position-specific scoring matrices. J Mol Biol (1999) 33.07

Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae. Microbiol Mol Biol Rev (1999) 19.25

Mobile elements: drivers of genome evolution. Science (2004) 11.78

Correct integration of retroviral DNA in vitro. Cell (1987) 10.73

Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res (1994) 10.72

Reverse transcription of R2Bm RNA is primed by a nick at the chromosomal target site: a mechanism for non-LTR retrotransposition. Cell (1993) 8.99

Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell (2005) 7.29

Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases. Science (1994) 6.69

High-frequency P element loss in Drosophila is homolog dependent. Cell (1990) 6.64

GenTHREADER: an efficient and reliable protein fold recognition method for genomic sequences. J Mol Biol (1999) 6.42

Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis. Cell (2005) 5.43

Mechanisms of site-specific recombination. Annu Rev Biochem (2006) 4.29

PiggyBac transposon-mediated gene transfer in human cells. Mol Ther (2007) 4.07

Three-dimensional structure of the Tn5 synaptic complex transposition intermediate. Science (2000) 4.00

Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses. Virology (1989) 3.91

Rolling-circle transposons in eukaryotes. Proc Natl Acad Sci U S A (2001) 3.81

Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus. Cell (1986) 3.77

Similarities between initiation of V(D)J recombination and retroviral integration. Science (1996) 3.64

The PHD finger, a nuclear protein-interaction domain. Trends Biochem Sci (2005) 3.56

Transposition of hAT elements links transposable elements and V(D)J recombination. Nature (2004) 3.00

Turning junk into gold: domestication of transposable elements and the creation of new genes in eukaryotes. Bioessays (2006) 2.71

High-efficiency transformation of Plasmodium falciparum by the lepidopteran transposable element piggyBac. Proc Natl Acad Sci U S A (2005) 2.48

The origin of footprints of the Tc1 transposon of Caenorhabditis elegans. EMBO J (1991) 2.32

Patching broken chromosomes with extranuclear cellular DNA. Mol Cell (1999) 2.18

Transposable elements and host genome evolution. Trends Ecol Evol (2000) 2.13

In vitro transposition of bacteriophage Mu: a biochemical approach to a novel replication reaction. Cell (1983) 2.08

Tn10 transposition via a DNA hairpin intermediate. Cell (1998) 2.05

Comparative architecture of transposase and integrase complexes. Nat Struct Biol (2001) 1.99

Assay for movement of Lepidopteran transposon IFP2 in insect cells using a baculovirus genome as a target DNA. Virology (1995) 1.90

piggyBac internal sequences are necessary for efficient transformation of target genomes. Insect Mol Biol (2005) 1.76

Molecular evolutionary analysis of the widespread piggyBac transposon family and related "domesticated" sequences. Mol Genet Genomics (2003) 1.76

Hairpin formation in Tn5 transposition. J Biol Chem (1999) 1.73

Tn5 as a model for understanding DNA transposition. Mol Microbiol (2003) 1.69

Molecular architecture of a eukaryotic DNA transposase. Nat Struct Mol Biol (2005) 1.64

The three chemical steps of Tn10/IS10 transposition involve repeated utilization of a single active site. Cell (1996) 1.52

Mechanism of Mos1 transposition: insights from structural analysis. EMBO J (2006) 1.48

Transposition of maize Ac/Ds transposable elements in the yeast Saccharomyces cerevisiae. Nat Genet (2000) 1.43

piggyBac transposon mediated transgenesis of the human blood fluke, Schistosoma mansoni. FASEB J (2007) 1.42

Mechanism of transposition of bacteriophage Mu: polarity of the strand transfer reaction at the initiation of transposition. Cell (1984) 1.41

Organization and dynamics of the Mu transpososome: recombination by communication between two active sites. Genes Dev (1999) 1.36

Transgenic planarian lines obtained by electroporation using transposon-derived vectors and an eye-specific GFP marker. Proc Natl Acad Sci U S A (2003) 1.20

Step-arrest mutants of phage Mu transposase. Implications in DNA-protein assembly, Mu end cleavage, and strand transfer. J Biol Chem (1995) 1.19

Single-stranded DNA intermediates in IS91 rolling-circle transposition. Mol Microbiol (2001) 1.19

Transposition of the piggyBac element in embryos of Drosophila melanogaster, Aedes aegypti and Trichoplusia ni. Mol Gen Genet (1999) 1.18

Diverse DNA transposons in rotifers of the class Bdelloidea. Proc Natl Acad Sci U S A (2005) 1.16

Amino acid residues in Rag1 crucial for DNA hairpin formation. Nat Struct Mol Biol (2006) 1.16

Excision of IS492 requires flanking target sequences and results in circle formation in Pseudoalteromonas atlantica. J Bacteriol (1999) 1.07

DNA binding and phasing analyses of Tn5 transposase and a monomeric variant. Nucleic Acids Res (1997) 1.05

Evolution of the Xenopus piggyBac transposon family TxpB: domesticated and untamed strategies of transposon subfamilies. Mol Biol Evol (2007) 0.99

Base-flipping dynamics in a DNA hairpin processing reaction. Nucleic Acids Res (2007) 0.98

Analysis of the cis-acting DNA elements required for piggyBac transposable element excision. Mol Gen Genet (1997) 0.97

Flanking host sequences can exert an inhibitory effect on the cleavage step of the in vitro mu DNA strand transfer reaction. J Biol Chem (1992) 0.95

A high-throughput assay for Tn5 Tnp-induced DNA cleavage. Nucleic Acids Res (2004) 0.78

Articles by these authors

Transposition of hAT elements links transposable elements and V(D)J recombination. Nature (2004) 3.00

A hyperactive piggyBac transposase for mammalian applications. Proc Natl Acad Sci U S A (2011) 2.68

An open letter to Elias Zerhouni. Science (2005) 2.19

Molecular architecture of a eukaryotic DNA transposase. Nat Struct Mol Biol (2005) 1.64

Fast, easy and efficient: site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion event. BMC Microbiol (2006) 1.54

DNA transposon Hermes inserts into DNA in nucleosome-free regions in vivo. Proc Natl Acad Sci U S A (2010) 1.50

Mobilization of giant piggyBac transposons in the mouse genome. Nucleic Acids Res (2011) 1.49

Definition of the Escherichia coli MC4100 genome by use of a DNA array. J Bacteriol (2003) 1.47

Multiple waves of recent DNA transposon activity in the bat, Myotis lucifugus. Genome Res (2008) 1.42

Tn7-based genome-wide random insertional mutagenesis of Candida glabrata. Genome Res (2003) 1.36

piggyBac transposase tools for genome engineering. Proc Natl Acad Sci U S A (2013) 1.35

Protein kinase A regulates constitutive expression of small heat-shock genes in an Msn2/4p-independent and Hsf1p-dependent manner in Saccharomyces cerevisiae. Genetics (2004) 1.30

Hyperactive piggyBac gene transfer in human cells and in vivo. Hum Gene Ther (2011) 1.21

Insertion site preference of Mu, Tn5, and Tn7 transposons. Mob DNA (2012) 1.17

Large-scale mutagenesis of the yeast genome using a Tn7-derived multipurpose transposon. Genome Res (2004) 1.11

Formation of a nucleoprotein complex containing Tn7 and its target DNA regulates transposition initiation. EMBO J (2002) 1.04

The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe. Genetics (2007) 1.02

Phylogenetic and functional characterization of the hAT transposon superfamily. Genetics (2011) 1.00

The mosquito Aedes aegypti has a large genome size and high transposable element load but contains a low proportion of transposon-specific piRNAs. BMC Genomics (2011) 0.97

Site-specific Tn7 transposition into the human genome. Nucleic Acids Res (2005) 0.97

Alternative interactions between the Tn7 transposase and the Tn7 target DNA binding protein regulate target immunity and transposition. EMBO J (2003) 0.97

A resurrected mammalian hAT transposable element and a closely related insect element are highly active in human cell culture. Proc Natl Acad Sci U S A (2012) 0.94

A Hyperactive Transposase Promotes Persistent Gene Transfer of a piggyBac DNA Transposon. Mol Ther Nucleic Acids (2012) 0.93

Functional characterization of an active Rag-like transposase. Nat Struct Mol Biol (2012) 0.92

Functional characterization of piggyBat from the bat Myotis lucifugus unveils an active mammalian DNA transposon. Proc Natl Acad Sci U S A (2012) 0.90

Comparative analysis of the recently discovered hAT transposon TcBuster in human cells. PLoS One (2012) 0.87

The carboxy-terminal portion of TnsC activates the Tn7 transposase through a specific interaction with TnsA. EMBO J (2004) 0.84

Tn7-mediated mutagenesis of Saccharomyces cerevisiae genomic DNA in vitro. Methods Enzymol (2002) 0.84

Architecture of the Tn7 posttransposition complex: an elaborate nucleoprotein structure. J Mol Biol (2010) 0.82

Integration profiling of gene function with dense maps of transposon integration. Genetics (2013) 0.79

Direct interaction between the TnsA and TnsB subunits controls the heteromeric Tn7 transposase. Proc Natl Acad Sci U S A (2013) 0.78

New wrinkles and folds in site-specific recombination. Mol Cell (2005) 0.76

Purification, crystallization and preliminary crystallographic analysis of the Hermes transposase. Acta Crystallogr Sect F Struct Biol Cryst Commun (2005) 0.76

IPB7 transposase behavior in Drosophila melanogaster and Aedes aegypti. Insect Biochem Mol Biol (2013) 0.75