Published in Proc Natl Acad Sci U S A on April 08, 2008
Reaction mechanisms of thiamin diphosphate enzymes: defining states of ionization and tautomerization of the cofactor at individual steps. FEBS J (2009) 1.28
Experimental observation of thiamin diphosphate-bound intermediates on enzymes and mechanistic information derived from these observations. Bioorg Chem (2005) 1.21
Assignment of function to histidines 260 and 298 by engineering the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase complex; substitutions that lead to acceptance of substrates lacking the 5-carboxyl group. Biochemistry (2011) 0.89
Snapshot of a reaction intermediate: analysis of benzoylformate decarboxylase in complex with a benzoylphosphonate inhibitor. Biochemistry (2009) 0.89
Construction and flow cytometric screening of targeted enzyme libraries. Nat Protoc (2009) 0.89
Detection and time course of formation of major thiamin diphosphate-bound covalent intermediates derived from a chromophoric substrate analogue on benzoylformate decarboxylase. Biochemistry (2009) 0.81
Mechanistic insights into the bifunctional non-heme iron oxygenase carbapenem synthase by active site saturation mutagenesis. J Am Chem Soc (2013) 0.80
Catalytic effects of mutations of distant protein residues in human DNA polymerase β: theory and experiment. Biochemistry (2012) 0.80
Catalysis in Enzymatic Decarboxylations: Comparison of Selected Cofactor-dependent and Cofactor-independent Examples. ACS Catal (2013) 0.78
Random mutagenesis MAPPIT analysis identifies binding sites for Vif and Gag in both cytidine deaminase domains of Apobec3G. PLoS One (2012) 0.77
In silico saturation mutagenesis and docking screening for the analysis of protein-ligand interaction: the Endothelial Protein C Receptor case study. BMC Bioinformatics (2009) 0.75
Perturbation of the monomer-monomer interfaces of the benzoylformate decarboxylase tetramer. Biochemistry (2014) 0.75
GenTHREADER: an efficient and reliable protein fold recognition method for genomic sequences. J Mol Biol (1999) 6.42
Improvement of the GenTHREADER method for genomic fold recognition. Bioinformatics (2003) 3.70
The enzymatic conversion of mandelic acid to benzoic acid. III. Fractionation and properties of the soluble enzymes. J Bacteriol (1953) 3.10
The catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography. Nat Chem Biol (2006) 2.06
Combinatorial alanine-scanning. Curr Opin Chem Biol (2001) 1.82
Engineering of glycosidases and glycosyltransferases. Curr Opin Chem Biol (2006) 1.49
NMR analysis of covalent intermediates in thiamin diphosphate enzymes. Biochemistry (2003) 1.48
Elucidation of the chemistry of enzyme-bound thiamin diphosphate prior to substrate binding: defining internal equilibria among tautomeric and ionization states. Biochemistry (2007) 1.37
The crystal structure of benzoylformate decarboxylase at 1.6 A resolution: diversity of catalytic residues in thiamin diphosphate-dependent enzymes. Biochemistry (1998) 1.22
Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida. Protein Eng Des Sel (2005) 1.10
Catalytic acid-base groups in yeast pyruvate decarboxylase. 2. Insights into the specific roles of D28 and E477 from the rates and stereospecificity of formation of carboligase side products. Biochemistry (2001) 1.09
Enzyme catalysis: removing chemically 'essential' residues by site-directed mutagenesis. Trends Biochem Sci (2001) 1.07
Remarkable stabilization of zwitterionic intermediates may account for a billion-fold rate acceleration by thiamin diphosphate-dependent decarboxylases. Biochemistry (1999) 1.06
Snapshot of a key intermediate in enzymatic thiamin catalysis: crystal structure of the alpha-carbanion of (alpha,beta-dihydroxyethyl)-thiamin diphosphate in the active site of transketolase from Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (2002) 1.05
Evidence for dramatic acceleration of a C-H bond ionization rate in thiamin diphosphate enzymes by the protein environment. Biochemistry (2005) 0.96
Spectroscopic detection of transient thiamin diphosphate-bound intermediates on benzoylformate decarboxylase. Biochemistry (2000) 0.95
Structural and kinetic analysis of catalysis by a thiamin diphosphate-dependent enzyme, benzoylformate decarboxylase. Biochemistry (2003) 0.94
Kinetics and mechanism of benzoylformate decarboxylase using 13C and solvent deuterium isotope effects on benzoylformate and benzoylformate analogues. Biochemistry (1988) 0.94
Site-directed mutagenesis of the ionizable groups in the active site of Zymomonas mobilis pyruvate decarboxylase: effect on activity and pH dependence. Eur J Biochem (2001) 0.92
Directed evolution: an approach to engineer enzymes. Crit Rev Biotechnol (2006) 0.91
Exploring the active site of benzaldehyde lyase by modeling and mutagenesis. Biochim Biophys Acta (2005) 0.87
Determinants of substrate specificity in KdcA, a thiamin diphosphate-dependent decarboxylase. Bioorg Chem (2006) 0.83
Isolation and characterization of a benzoylformate decarboxylase and a NAD+/NADP+-dependent benzaldehyde dehydrogenase involved in D-phenylglycine metabolism in Pseudomonas stutzeri ST-201. Biochim Biophys Acta (2007) 0.82
Histidine to alanine mutants of human dihydroorotate dehydrogenase. Identification of a brequinar-resistant mutant enzyme. Biochem Pharmacol (1997) 0.81
Metabolism of mandelic acid by Neurospora crassa. Can J Microbiol (1977) 0.78
Elucidation of the chemistry of enzyme-bound thiamin diphosphate prior to substrate binding: defining internal equilibria among tautomeric and ionization states. Biochemistry (2007) 1.37
Relating structure to mechanism in creatine kinase. Crit Rev Biochem Mol Biol (2005) 1.31
Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida. Protein Eng Des Sel (2005) 1.10
The 2.1 A structure of Torpedo californica creatine kinase complexed with the ADP-Mg(2+)-NO(3)(-)-creatine transition-state analogue complex. Biochemistry (2002) 1.09
Novel mechanism of inhibition of HIV-1 reverse transcriptase by a new non-nucleoside analog, KM-1. J Biol Chem (2004) 1.00
Structural and kinetic analysis of catalysis by a thiamin diphosphate-dependent enzyme, benzoylformate decarboxylase. Biochemistry (2003) 0.94
Mechanism-based inactivation of thioredoxin reductase from Plasmodium falciparum by Mannich bases. Implication for cytotoxicity. Biochemistry (2003) 0.93
Structural, mutagenic, and kinetic analysis of the binding of substrates and inhibitors of human phenylethanolamine N-methyltransferase. J Med Chem (2005) 0.93
Characterization of benzaldehyde lyase from Pseudomonas fluorescens: A versatile enzyme for asymmetric C-C bond formation. Bioorg Chem (2006) 0.90
Probing the active center of benzaldehyde lyase with substitutions and the pseudosubstrate analogue benzoylphosphonic acid methyl ester. Biochemistry (2008) 0.89
Snapshot of a reaction intermediate: analysis of benzoylformate decarboxylase in complex with a benzoylphosphonate inhibitor. Biochemistry (2009) 0.89
Mode of binding of methyl acceptor substrates to the adrenaline-synthesizing enzyme phenylethanolamine N-methyltransferase: implications for catalysis. Biochemistry (2005) 0.89
Molecular recognition of sub-micromolar inhibitors by the epinephrine-synthesizing enzyme phenylethanolamine N-methyltransferase. J Med Chem (2004) 0.88
Structural relationship between the active sites of β-lactam-recognizing and amidase signature enzymes: convergent evolution? Biochemistry (2010) 0.88
Mandelamide hydrolase from Pseudomonas putida: characterization of a new member of the amidase signature family. Biochemistry (2004) 0.88
Exploring the active site of benzaldehyde lyase by modeling and mutagenesis. Biochim Biophys Acta (2005) 0.87
Mechanism of benzaldehyde lyase studied via thiamin diphosphate-bound intermediates and kinetic isotope effects. Biochemistry (2008) 0.86
Molecular recognition of physiological substrate noradrenaline by the adrenaline-synthesizing enzyme PNMT and factors influencing its methyltransferase activity. Biochem J (2009) 0.86
Fragment-based screening by X-ray crystallography, MS and isothermal titration calorimetry to identify PNMT (phenylethanolamine N-methyltransferase) inhibitors. Biochem J (2010) 0.86
The reaction mechanism of phenylethanolamine N-methyltransferase: a density functional theory study. Biochim Biophys Acta (2009) 0.85
A novel mechanism for inhibition of HIV-1 reverse transcriptase. Bioorg Chem (2002) 0.85
Enzyme adaptation to inhibitor binding: a cryptic binding site in phenylethanolamine N-methyltransferase. J Med Chem (2007) 0.84
Determinants of substrate specificity in KdcA, a thiamin diphosphate-dependent decarboxylase. Bioorg Chem (2006) 0.83
Isolation and characterization of a benzoylformate decarboxylase and a NAD+/NADP+-dependent benzaldehyde dehydrogenase involved in D-phenylglycine metabolism in Pseudomonas stutzeri ST-201. Biochim Biophys Acta (2007) 0.82
Phenylethanolamine N-methyltransferase inhibition: re-evaluation of kinetic data. Bioorg Med Chem Lett (2004) 0.82
Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae. FEBS J (2011) 0.81
Mechanism-based inactivation of benzoylformate decarboxylase, a thiamin diphosphate-dependent enzyme. J Am Chem Soc (2007) 0.81
Detection and time course of formation of major thiamin diphosphate-bound covalent intermediates derived from a chromophoric substrate analogue on benzoylformate decarboxylase. Biochemistry (2009) 0.81
Substrate specificity in thiamin diphosphate-dependent decarboxylases. Bioorg Chem (2011) 0.80
Isoleucine 69 and valine 325 form a specificity pocket in human muscle creatine kinase. Biochemistry (2004) 0.80
Engineering the substrate binding site of benzoylformate decarboxylase. Biochemistry (2009) 0.80
Expression of Torpedo californica creatine kinase in Escherichia coli and purification from inclusion bodies. Protein Expr Purif (2002) 0.80
A bulky hydrophobic residue is not required to maintain the V-conformation of enzyme-bound thiamin diphosphate. Biochemistry (2013) 0.79
Identification of charge transfer transitions related to thiamin-bound intermediates on enzymes provides a plethora of signatures useful in mechanistic studies. Biochemistry (2014) 0.79
Kinetic and pH studies on human phenylethanolamine N-methyltransferase. Arch Biochem Biophys (2013) 0.79
Loop movement and catalysis in creatine kinase. IUBMB Life (2005) 0.78
Active-site engineering of benzaldehyde lyase shows that a point mutation can confer both new reactivity and susceptibility to mechanism-based inhibition. J Am Chem Soc (2010) 0.78
Crystallization of PNMT, the adrenaline-synthesizing enzyme, is critically dependent on a high protein concentration. Acta Crystallogr D Biol Crystallogr (2002) 0.77
Using site-saturation mutagenesis to explore mechanism and substrate specificity in thiamin diphosphate-dependent enzymes. FEBS J (2013) 0.77
Physical, kinetic and spectrophotometric studies of a NAD(P)-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633. Biochim Biophys Acta (2008) 0.77
Disulfide-linked dimers of human adrenaline synthesizing enzyme PNMT are catalytically active. Biochim Biophys Acta (2005) 0.77
Exploring the role of the active site cysteine in human muscle creatine kinase. Biochemistry (2006) 0.77
Time-dependent inactivation of human phenylethanolamine N-methyltransferase by 7-isothiocyanatotetrahydroisoquinoline. Bioorg Med Chem Lett (2009) 0.76
Identification and characterization of a mandelamide hydrolase and an NAD(P)+-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633. J Bacteriol (2003) 0.75
Perturbation of the monomer-monomer interfaces of the benzoylformate decarboxylase tetramer. Biochemistry (2014) 0.75
Extended reaction scope of thiamine diphosphate dependent cyclohexane-1,2-dione hydrolase: from C-C bond cleavage to C-C bond ligation. Angew Chem Int Ed Engl (2014) 0.75
Using directed evolution to probe the substrate specificity of mandelamide hydrolase. Protein Eng Des Sel (2008) 0.75
Heterogeneity of Escherichia coli-expressed human muscle creatine kinase. IUBMB Life (2006) 0.75