Published in Protein Expr Purif on August 15, 2008
Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins. Nat Methods (2010) 2.77
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Practical cell-free protein synthesis system using purified wheat embryos. Nat Protoc (2010) 1.47
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A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles. Protein Expr Purif (2007) 0.90
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A plasminogen-like protein selectively degrades stearoyl-CoA desaturase in liver microsomes. J Biol Chem (2003) 0.89
Molecular differences caused by differentiation of 3T3-L1 preadipocytes in the presence of either dehydroepiandrosterone (DHEA) or 7-oxo-DHEA. Biochemistry (2002) 0.88
Construction of an efficient expression vector for coupled transcription/translation in a wheat germ cell-free system. Nucleic Acids Symp Ser (2000) 0.87
Simultaneous expression and maturation of the iron-sulfur protein ferredoxin in a cell-free system. Biotechnol Bioeng (2006) 0.87
Identification of Rv3230c as the NADPH oxidoreductase of a two-protein DesA3 acyl-CoA desaturase in Mycobacterium tuberculosis H37Rv. Biochemistry (2006) 0.86
Detection of structural changes in a cofactor binding protein by using a wheat germ cell-free protein synthesis system coupled with unnatural amino acid probing. Proteins (2007) 0.83
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Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins. Nat Methods (2010) 2.77
A combined approach to improving large-scale production of tobacco etch virus protease. Protein Expr Purif (2007) 2.62
Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium. Protein Expr Purif (2005) 2.02
Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination. Protein Expr Purif (2005) 1.65
High-throughput purification and quality assurance of Arabidopsis thaliana proteins for eukaryotic structural genomics. J Struct Funct Genomics (2005) 1.65
Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination. J Struct Funct Genomics (2005) 1.64
Structure and mechanism of mouse cysteine dioxygenase. Proc Natl Acad Sci U S A (2006) 1.59
Effects of sterculic acid on stearoyl-CoA desaturase in differentiating 3T3-L1 adipocytes. Biochem Biophys Res Commun (2003) 1.59
Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition. Biotechnol Prog (2007) 1.51
Structural consequences of effector protein complex formation in a diiron hydroxylase. Proc Natl Acad Sci U S A (2008) 1.47
High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system. Protein Expr Purif (2005) 1.38
Characterization of the nitrosyl adduct of substrate-bound mouse cysteine dioxygenase by electron paramagnetic resonance: electronic structure of the active site and mechanistic implications. Biochemistry (2007) 1.34
Combined participation of hydroxylase active site residues and effector protein binding in a para to ortho modulation of toluene 4-monooxygenase regiospecificity. Biochemistry (2002) 1.29
Comparison of cell-based and cell-free protocols for producing target proteins from the Arabidopsis thaliana genome for structural studies. Proteins (2005) 1.26
Solution structures of spinach acyl carrier protein with decanoate and stearate. Biochemistry (2006) 1.23
Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces. Sci Rep (2013) 1.16
Global gene expression patterns in Clostridium thermocellum as determined by microarray analysis of chemostat cultures on cellulose or cellobiose. Appl Environ Microbiol (2010) 1.14
Cell-free translation of integral membrane proteins into unilamelar liposomes. Methods Enzymol (2009) 1.10
Insight into the mechanism of aromatic hydroxylation by toluene 4-monooxygenase by use of specifically deuterated toluene and p-xylene. Proc Natl Acad Sci U S A (2003) 1.07
N-isotope effects on the Raman spectra of Fe(2)S(2) ferredoxin and Rieske ferredoxin: evidence for structural rigidity of metal sites. J Biol Inorg Chem (2002) 1.06
Coordinating the impact of structural genomics on the human α-helical transmembrane proteome. Nat Struct Mol Biol (2013) 1.05
Spectroscopic and computational characterization of substrate-bound mouse cysteine dioxygenase: nature of the ferrous and ferric cysteine adducts and mechanistic implications. Biochemistry (2010) 0.99
Small-scale, semi-automated purification of eukaryotic proteins for structure determination. J Struct Funct Genomics (2007) 0.96
Structures of proteins of biomedical interest from the Center for Eukaryotic Structural Genomics. J Struct Funct Genomics (2007) 0.96
Cellulolytic Streptomyces strains associated with herbivorous insects share a phylogenetically linked capacity to degrade lignocellulose. Appl Environ Microbiol (2014) 0.94
Fluorescence anisotropy assay for proteolysis of specifically labeled fusion proteins. Anal Biochem (2005) 0.94
Structure of human J-type co-chaperone HscB reveals a tetracysteine metal-binding domain. J Biol Chem (2008) 0.94
Geometric and electronic structure studies of the binuclear nonheme ferrous active site of toluene-4-monooxygenase: parallels with methane monooxygenase and insight into the role of the effector proteins in O2 activation. J Am Chem Soc (2008) 0.94
Cell-free protein synthesis technology in NMR high-throughput structure determination. Methods Mol Biol (2010) 0.94
Structure of T4moC, the Rieske-type ferredoxin component of toluene 4-monooxygenase. Acta Crystallogr D Biol Crystallogr (2006) 0.93
Role for threonine 201 in the catalytic cycle of the soluble diiron hydroxylase toluene 4-monooxygenase. Biochemistry (2009) 0.93
X-ray structure of putative acyl-ACP desaturase DesA2 from Mycobacterium tuberculosis H37Rv. Protein Sci (2005) 0.93
Crystallographic and catalytic studies of the peroxide-shunt reaction in a diiron hydroxylase. Biochemistry (2009) 0.92
Production in two-liter beverage bottles of proteins for NMR structure determination labeled with either 15N- or 13C-15N. J Struct Funct Genomics (2004) 0.91
Expression, purification, and physical characterization of Escherichia coli lipoyl(octanoyl)transferase. Protein Expr Purif (2005) 0.91
Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes. Sci Rep (2013) 0.91
The length of the bound fatty acid influences the dynamics of the acyl carrier protein and the stability of the thioester bond. Biochemistry (2010) 0.90
Flexi vector cloning. Methods Mol Biol (2009) 0.90
Cell-free synthesis and functional characterization of sphingolipid synthases from parasitic trypanosomatid protozoa. J Biol Chem (2010) 0.90
A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles. Protein Expr Purif (2007) 0.90
Fluorescence anisotropy studies of enzyme-substrate complex formation in stearoyl-ACP desaturase. Biochemistry (2002) 0.89
Remarkable aliphatic hydroxylation by the diiron enzyme toluene 4-monooxygenase in reactions with radical or cation diagnostic probes norcarane, 1,1-dimethylcyclopropane, and 1,1-diethylcyclopropane. Biochemistry (2004) 0.89
Reaction mechanisms of non-heme diiron hydroxylases characterized in whole cells. J Inorg Biochem (2005) 0.89
Redox and functional analysis of the Rieske ferredoxin component of the toluene 4-monooxygenase. Biochemistry (2007) 0.89
Crystallographic analysis of active site contributions to regiospecificity in the diiron enzyme toluene 4-monooxygenase. Biochemistry (2012) 0.89
Molecular differences caused by differentiation of 3T3-L1 preadipocytes in the presence of either dehydroepiandrosterone (DHEA) or 7-oxo-DHEA. Biochemistry (2002) 0.88
X-ray structure of ILL2, an auxin-conjugate amidohydrolase from Arabidopsis thaliana. Proteins (2009) 0.88
One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies. Protein Sci (2009) 0.88
Crystal structures and functional studies of T4moD, the toluene 4-monooxygenase catalytic effector protein. Biochemistry (2005) 0.88
Mutations in FLS2 Ser-938 dissect signaling activation in FLS2-mediated Arabidopsis immunity. PLoS Pathog (2013) 0.88
The Center for Eukaryotic Structural Genomics. J Struct Funct Genomics (2009) 0.87
A graphical approach to tracking and reporting target status in structural genomics. J Struct Funct Genomics (2008) 0.87
Preparation of isotopically labeled spinach acyl-acyl carrier protein for NMR structural studies. Protein Expr Purif (2005) 0.86
Identification of Rv3230c as the NADPH oxidoreductase of a two-protein DesA3 acyl-CoA desaturase in Mycobacterium tuberculosis H37Rv. Biochemistry (2006) 0.86
Transformation of RDX and other energetic compounds by xenobiotic reductases XenA and XenB. Appl Microbiol Biotechnol (2009) 0.86
Component interactions and implications for complex formation in the multicomponent toluene 4-monooxygenase. Biochemistry (2006) 0.86
Identification of the binding region of the [2Fe-2S] ferredoxin in stearoyl-acyl carrier protein desaturase: insight into the catalytic complex and mechanism of action. Biochemistry (2006) 0.86
X-ray structure of Arabidopsis At2g06050, 12-oxophytodienoate reductase isoform 3. Proteins (2005) 0.85
Desaturation, chain scission, and register-shift of oxygen-substituted fatty acids during reaction with stearoyl-ACP desaturase. Biochemistry (2002) 0.85
Rapid kinetic characterization of glycosyl hydrolases based on oxime derivatization and nanostructure-initiator mass spectrometry (NIMS). ACS Chem Biol (2014) 0.85
Cell-free translation of biofuel enzymes. Methods Mol Biol (2014) 0.85
X-ray structure of Danio rerio secretagogin: A hexa-EF-hand calcium sensor. Proteins (2009) 0.85
Spectroscopic and computational characterization of the NO adduct of substrate-bound Fe(II) cysteine dioxygenase: insights into the mechanism of O2 activation. Biochemistry (2013) 0.84
Structure of cellobiose phosphorylase from Clostridium thermocellum in complex with phosphate. Acta Crystallogr Sect F Struct Biol Cryst Commun (2011) 0.84
Fusion of dioxygenase and lignin-binding domains in a novel secreted enzyme from cellulolytic Streptomyces sp. SirexAA-E. J Biol Chem (2013) 0.83
Complete genome sequence of the cellulose-degrading bacterium Cellulosilyticum lentocellum. J Bacteriol (2011) 0.83
Solution structure of T4moC, the Rieske ferredoxin component of the toluene 4-monooxygenase complex. J Biol Inorg Chem (2004) 0.83
Structural and functional characterization of a novel phosphatase from the Arabidopsis thaliana gene locus At1g05000. Proteins (2008) 0.83
Spectroscopic and computational investigation of iron(III) cysteine dioxygenase: implications for the nature of the putative superoxo-Fe(III) intermediate. Biochemistry (2014) 0.82
Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies. J Struct Funct Genomics (2015) 0.82
Soluble expression and purification of the oxidoreductase component of toluene 4-monooxygenase. Protein Expr Purif (2007) 0.82
Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins. N Biotechnol (2010) 0.81
Cell-free production of integral membrane aspartic acid proteases reveals zinc-dependent methyltransferase activity of the Pseudomonas aeruginosa prepilin peptidase PilD. Microbiologyopen (2012) 0.80
Chain cleavage and sulfoxidation of thiastearoyl-ACP upon reaction with stearoyl-ACP desaturase. Biochemistry (2003) 0.80
Sulfate requirement for heterotrophic growth of "Ferroplasma acidarmanus" strain fer1. Res Microbiol (2005) 0.80
Cell-free protein synthesis for functional and structural studies. Methods Mol Biol (2014) 0.80
X-ray structure of a soluble Rieske-type ferredoxin from Mus musculus. Acta Crystallogr D Biol Crystallogr (2008) 0.79
Redox reactions of dehydroepiandrosterone and its metabolites in differentiating 3T3-L1 adipocytes: A liquid chromatographic-mass spectrometric study. Arch Biochem Biophys (2006) 0.79
Rapid-mix and chemical quench studies of ferredoxin-reduced stearoyl-acyl carrier protein desaturase. Biochemistry (2003) 0.79
Discovery of sarcosine dimethylglycine methyltransferase from Galdieria sulphuraria. Proteins (2009) 0.79
Crystallization and preliminary analysis of xenobiotic reductase B from Pseudomonas fluorescens I-C. Acta Crystallogr D Biol Crystallogr (2004) 0.78
Improved expression and purification of sigma 1 receptor fused to maltose binding protein by alteration of linker sequence. Protein Expr Purif (2013) 0.78
Oxygen-18 tracer studies of enzyme reactions with radical/cation diagnostic probes. Biochem Biophys Res Commun (2005) 0.78
Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family. Biochemistry (2011) 0.78
In vivo inactivation of the mycobacterial integral membrane stearoyl coenzyme A desaturase DesA3 by a C-terminus-specific degradation process. J Bacteriol (2008) 0.78
Automated cell-free protein production methods for structural studies. Methods Mol Biol (2014) 0.77
Crystal structure of At2g03760, a putative steroid sulfotransferase from Arabidopsis thaliana. Proteins (2004) 0.77
Structural architecture of Galdieria sulphuraria DCN1L. Proteins (2011) 0.77
The structure at 2.4 A resolution of the protein from gene locus At3g21360, a putative Fe(II)/2-oxoglutarate-dependent enzyme from Arabidopsis thaliana. Acta Crystallogr Sect F Struct Biol Cryst Commun (2005) 0.77
Crystal structure of the protein from gene At3g17210 of Arabidopsis thaliana. Proteins (2004) 0.76
Structure-guided analysis of catalytic specificity of the abundantly secreted chitosanase SACTE_5457 from Streptomyces sp. SirexAA-E. Proteins (2014) 0.76
Crystallization and preliminary analysis of xenobiotic reductase A and ligand complexes from Pseudomonas putida II-B. Acta Crystallogr D Biol Crystallogr (2004) 0.76
Crystal structure of Arabidopsis thaliana 12-oxophytodienoate reductase isoform 3 in complex with 8-iso prostaglandin A(1). Proteins (2011) 0.75
Crystallization and preliminary analysis of native and N-terminal truncated isoforms of toluene-4-monooxygenase catalytic effector protein. Acta Crystallogr D Biol Crystallogr (2003) 0.75
Crystal structure of gene locus At3g16990 from Arabidopsis thaliana. Proteins (2004) 0.75
A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres. Chem Commun (Camb) (2013) 0.75