Published in Proc Natl Acad Sci U S A on October 30, 2002
Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. Infect Immun (2008) 1.87
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Practical cell-free protein synthesis system using purified wheat embryos. Nat Protoc (2010) 1.47
Molecular architecture of the human Prp19/CDC5L complex. Mol Cell Biol (2010) 1.44
Creating protein models from electron-density maps using particle-filtering methods. Bioinformatics (2007) 1.37
Novel protein fold discovered in the PabI family of restriction enzymes. Nucleic Acids Res (2007) 1.22
In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state. Proc Natl Acad Sci U S A (2004) 1.19
Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex. Protein Expr Purif (2008) 1.17
A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis. BMC Plant Biol (2009) 1.11
Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling. BMC Plant Biol (2010) 1.10
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Discovery of a novel restriction endonuclease by genome comparison and application of a wheat-germ-based cell-free translation assay: PabI (5'-GTA/C) from the hyperthermophilic archaeon Pyrococcus abyssi. Nucleic Acids Res (2005) 1.03
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Characterization of a caspase-3-substrate kinome using an N- and C-terminally tagged protein kinase library produced by a cell-free system. Cell Death Dis (2010) 1.00
Involvement of hepatitis C virus NS5A hyperphosphorylation mediated by casein kinase I-α in infectious virus production. J Virol (2014) 1.00
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Biochemical analysis of the substrate specificity and sequence preference of endonuclease IV from bacteriophage T4, a dC-specific endonuclease implicated in restriction of dC-substituted T4 DNA synthesis. Nucleic Acids Res (2006) 0.95
Simple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis. J Proteome Res (2010) 0.94
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Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system. BMC Biotechnol (2011) 0.84
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A member of the CPW-WPC protein family is expressed in and localized to the surface of developing ookinetes. Malar J (2013) 0.81
Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins. BMC Biotechnol (2010) 0.81
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Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system. BMC Biotechnol (2014) 0.80
ASK1 restores the antiviral activity of APOBEC3G by disrupting HIV-1 Vif-mediated counteraction. Nat Commun (2015) 0.80
Wheat germ-based protein libraries for the functional characterisation of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase enzymes. BMC Plant Biol (2015) 0.79
A cell-free enzymatic activity assay for the evaluation of HIV-1 drug resistance to protease inhibitors. Front Microbiol (2015) 0.79
Cloning-independent expression and analysis of omega-transaminases by use of a cell-free protein synthesis system. Appl Environ Microbiol (2010) 0.79
Use of signal sequences as an in situ removable sequence element to stimulate protein synthesis in cell-free extracts. Nucleic Acids Res (2006) 0.78
Improved cell-free RNA and protein synthesis system. PLoS One (2014) 0.78
Specific in situ visualization of plasma cells producing antibodies against Porphyromonas gingivalis in gingival radicular cyst: application of the enzyme-labeled antigen method. J Histochem Cytochem (2011) 0.78
Identification of RFPL3 protein as a novel E3 ubiquitin ligase modulating the integration activity of human immunodeficiency virus, type 1 preintegration complex using a microtiter plate-based assay. J Biol Chem (2014) 0.78
Establishment of a Wheat Cell-Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins. PLoS One (2016) 0.78
Fine-tuning of protein domain boundary by minimizing potential coiled coil regions. J Biomol NMR (2006) 0.77
Apoglobin Stability Is the Major Factor Governing both Cell-free and in Vivo Expression of Holomyoglobin. J Biol Chem (2015) 0.77
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Characterization of RyDEN (C19orf66) as an Interferon-Stimulated Cellular Inhibitor against Dengue Virus Replication. PLoS Pathog (2016) 0.77
Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog. Parasit Vectors (2016) 0.76
Cell-free Escherichia coli-based system to screen for quorum-sensing molecules interacting with quorum receptor proteins of Streptomyces coelicolor. Appl Environ Microbiol (2009) 0.76
Distinct and site-specific phosphorylation of the retinoblastoma protein at serine 612 in differentiated cells. PLoS One (2014) 0.76
Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts. Proteome Sci (2010) 0.76
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Vesicular LL-37 contributes to inflammation of the lesional skin of palmoplantar pustulosis. PLoS One (2014) 0.75
Immunoscreening of Plasmodium falciparum proteins expressed in a wheat germ cell-free system reveals a novel malaria vaccine candidate. Sci Rep (2017) 0.75
Novel Autoantigens Associated with Lupus Nephritis. PLoS One (2015) 0.75
Enzyme-labeled Antigen Method: Development and Application of the Novel Approach for Identifying Plasma Cells Locally Producing Disease-specific Antibodies in Inflammatory Lesions. Acta Histochem Cytochem (2016) 0.75
A cell-free method for expressing and reconstituting membrane proteins enables functional characterization of the plant receptor-like protein kinase FERONIA. J Biol Chem (2017) 0.75
H11/HSPB8 Restricts HIV-2 Vpx to Restore the Anti-Viral Activity of SAMHD1. Front Microbiol (2016) 0.75
Novel type of adenylyl cyclase participates in tabtoxinine-β-lactam-induced cell death and occurrence of wildfire disease in Nicotiana benthamiana. Plant Signal Behav (2014) 0.75
The tumour suppressor APC promotes HIV-1 assembly via interaction with Gag precursor protein. Nat Commun (2017) 0.75
Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD. Biomark Insights (2015) 0.75
Involvement of PUF60 in Transcriptional and Post-transcriptional Regulation of Hepatitis B Virus Pregenomic RNA Expression. Sci Rep (2017) 0.75
Plant Aurora kinases interact with and phosphorylate transcription factors. J Plant Res (2016) 0.75
Cell-free expression, purification, and membrane reconstitution for NMR studies of the nonstructural protein 4B from hepatitis C virus. J Biomol NMR (2016) 0.75
High-yield production of "difficult-to-express" proteins in a continuous exchange cell-free system based on CHO cell lysates. Sci Rep (2017) 0.75
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Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. Infect Immun (2008) 1.87
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An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system. Proc Natl Acad Sci U S A (2002) 1.35
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A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis. BMC Plant Biol (2009) 1.11
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Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling. BMC Plant Biol (2010) 1.10
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Covalent circularization of exogenous RNA during incubation with a wheat embryo cell extract. Biochem Biophys Res Commun (2006) 0.79
High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system. Mol Biosyst (2014) 0.79
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Molecular and enzymatic characterization of XMRV protease by a cell-free proteolytic analysis. J Proteomics (2012) 0.78
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