Published in Biochemistry on October 08, 1991
Hammerhead ribozymes: importance of stem-loop II for activity. Proc Natl Acad Sci U S A (1993) 1.69
Ion-induced folding of the hammerhead ribozyme: a fluorescence resonance energy transfer study. EMBO J (1997) 1.57
Nuclease-resistant chimeric ribozymes containing deoxyribonucleotides and phosphorothioate linkages. Nucleic Acids Res (1993) 1.44
Function of specific 2'-hydroxyl groups of guanosines in a hammerhead ribozyme probed by 2' modifications. Proc Natl Acad Sci U S A (1992) 1.41
Importance of specific purine amino and hydroxyl groups for efficient cleavage by a hammerhead ribozyme. Proc Natl Acad Sci U S A (1992) 1.12
The synthesis of oligoribonucleotides containing O6-methylguanosine: the role of conserved guanosine residues in hammerhead ribozyme cleavage. Nucleic Acids Res (1993) 1.02
Design and specificity of hammerhead ribozymes against calretinin mRNA. Nucleic Acids Res (1993) 0.99
A ribozyme with DNA in the hybridising arms displays enhanced cleavage ability. Nucleic Acids Res (1992) 0.99
Enhancement of ribozyme catalytic activity by a contiguous oligodeoxynucleotide (facilitator) and by 2'-O-methylation. Nucleic Acids Res (1992) 0.98
The roles of the conserved pyrimidine bases in hammerhead ribozyme catalysis: evidence for a magnesium ion-binding site. Biochem J (1995) 0.94
Selection, design, and characterization of a new potentially therapeutic ribozyme. RNA (2002) 0.92
Discrimination of a single base change in a ribozyme using the gene for dihydrofolate reductase as a selective marker in Escherichia coli. Proc Natl Acad Sci U S A (1997) 0.86
Total chemical synthesis of a ribozyme derived from a group I intron. Proc Natl Acad Sci U S A (1995) 0.86
Probing the hammerhead ribozyme structure with ribonucleases. Nucleic Acids Res (1994) 0.85
Translation of 2'-modified mRNA in vitro and in vivo. Nucleic Acids Res (1994) 0.84
An oligodeoxyribonucleotide that supports catalytic activity in the hammerhead ribozyme domain. Nucleic Acids Res (1995) 0.83
The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA. Nucleic Acids Res (1985) 12.11
Nucleoside phosphorothioates. Annu Rev Biochem (1985) 7.55
Incorporation of phosphorothioate groups into fd and phi X174 DNA. Biochemistry (1977) 6.10
Inhibition of restriction endonuclease Nci I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis. Nucleic Acids Res (1986) 5.90
Deoxynucleoside [1-thio]triphosphates prevent proofreading during in vitro DNA synthesis. Proc Natl Acad Sci U S A (1981) 4.14
The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA. Nucleic Acids Res (1985) 3.65
5'-3' exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis. Nucleic Acids Res (1988) 3.41
Kinetic characterization of ribonuclease-resistant 2'-modified hammerhead ribozymes. Science (1991) 3.18
Guanosine 5'-O-(2-thiodiphosphate). An inhibitor of adenylate cyclase stimulation by guanine nucleotides and fluoride ions. J Biol Chem (1979) 3.12
Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site. Biochemistry (1984) 3.03
DNA and RNA sequence determination based on phosphorothioate chemistry. Science (1988) 2.95
Numbering system for the hammerhead. Nucleic Acids Res (1992) 2.74
The magnesium ion-dependent adenosine triphosphatase of myosin. Two-step processes of adenosine triphosphate association and adenosine diphosphate dissociation. Biochem J (1974) 2.57
Direct interaction of the rat unc-13 homologue Munc13-1 with the N terminus of syntaxin. J Biol Chem (1997) 2.50
A study of the mechanism of DNA polymerase I from Escherichia coli with diastereomeric phosphorothioate analogs of deoxyadenosine triphosphate. J Biol Chem (1979) 2.37
Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha-thiotriphosphates. Nucleic Acids Res (1988) 2.28
Incomplete primer extension during in vitro DNA amplification catalyzed by Taq polymerase; exploitation for DNA sequencing. Nucleic Acids Res (1989) 2.21
Synthesis and configurational analysis of a dinucleoside phosphate isotopically chiral at phosphorus. Stereochemical course of Penicillium citrum nuclease P1 reaction. Biochemistry (1983) 2.18
Properties of overexpressed phage T5 D15 exonuclease. Similarities with Escherichia coli DNA polymerase I 5'-3' exonuclease. J Biol Chem (1990) 2.17
Interaction of hydrolysis-resistant analogs of cyclic GMP with the phosphodiesterase and light-sensitive channel of retinal rod outer segments. Proc Natl Acad Sci U S A (1985) 2.04
Synthesis and properties of diastereoisomers of adenosine 5'-(O-1-thiotriphosphate) and adenosine 5'-(O-2-thiotriphosphate). Biochemistry (1976) 2.02
Incorporation of terminal phosphorothioates into oligonucleotides. Nucleic Acids Res (1998) 2.00
The structure, function and application of the hammerhead ribozyme. Eur J Biochem (1997) 1.94
Left-handed DNA: from synthetic polymers to chromosomes. J Biomol Struct Dyn (1983) 1.90
[Synthesis of oligodesoxynucleotides with phosphoric acid triester]. Chem Ber (1969) 1.89
Protection of oligonucleotide primers against degradation by DNA polymerase I. Biochemistry (1987) 1.87
Modified oligonucleotides: synthesis and strategy for users. Annu Rev Biochem (1998) 1.85
Absolute configuration of the diastereomers of adenosine 5'-O-(1-thiotriphosphate): consequences for the stereochemistry of polymerization by DNA-dependent RNA polymerase from Escherichia coli. Proc Natl Acad Sci U S A (1978) 1.82
Absolute stereochemistry of the second step of ribonuclease action. Nature (1970) 1.80
Potent 2'-amino-2'-deoxypyrimidine RNA inhibitors of basic fibroblast growth factor. Biochemistry (1995) 1.80
Two autolytic processing reactions of a satellite RNA proceed with inversion of configuration. Nucleic Acids Res (1990) 1.80
Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase. Nucleic Acids Res (1984) 1.77
Regional analysis of femorotibial cartilage loss in a subsample from the Osteoarthritis Initiative progression subcohort. Osteoarthritis Cartilage (2008) 1.76
Magnetic resonance imaging-based assessment of cartilage loss in severe osteoarthritis: accuracy, precision, and diagnostic value. Arthritis Rheum (2001) 1.76
Quantitative determination of joint incongruity and pressure distribution during simulated gait and cartilage thickness in the human hip joint. J Orthop Res (1999) 1.75
Nucleoside phosphorothioates. J Am Chem Soc (1970) 1.71
A three-dimensional model for the hammerhead ribozyme based on fluorescence measurements. Science (1994) 1.71
Geometry of the first step in the action of ribonuclease-A (in-line geometry-uridine2',3'-cyclic thiophosphate- 31 P NMR). Proc Natl Acad Sci U S A (1972) 1.71
Interaction of Escherichia coli tRNA(Ser) with its cognate aminoacyl-tRNA synthetase as determined by footprinting with phosphorothioate-containing tRNA transcripts. Proc Natl Acad Sci U S A (1991) 1.69
Hammerhead ribozymes: importance of stem-loop II for activity. Proc Natl Acad Sci U S A (1993) 1.69
MRI protocols for whole-organ assessment of the knee in osteoarthritis. Osteoarthritis Cartilage (2006) 1.68
Stereochemistry of polymerization by DNA-dependent RNA-polymerase from Escherichia coli: an investigation with a diastereomeric ATP-analogue. Proc Natl Acad Sci U S A (1976) 1.65
Diastereomers of 5'-O-adenosyl 3'-O-uridyl phosphorothioate: chemical synthesis and enzymatic properties. Biochemistry (1979) 1.65
Phosphorothioates in molecular biology. Trends Biochem Sci (1989) 1.62
Importance of exocyclic base functional groups of central core guanosines for hammerhead ribozyme activity. Biochemistry (1993) 1.62
Cleavage of phosphorothioate-substituted DNA by restriction endonucleases. J Biol Chem (1984) 1.61
Oligonucleotide duplexes containing 2'-amino-2'-deoxycytidines: thermal stability and chemical reactivity. Nucleic Acids Res (1994) 1.60
Age-related changes in the morphology and deformational behavior of knee joint cartilage. Arthritis Rheum (2001) 1.58
Stereochemistry of hydrolysis by snake venom phosphodiesterase. J Biol Chem (1979) 1.58
Dynamics of the RNA hairpin GNRA tetraloop. Biochemistry (2000) 1.47
Synthesis of d(GC) and d(CG) octamers containing alternating phosphorothioate linkages: effect of the phosphorothioate group on the B-Z transition. Biochemistry (1985) 1.46
Hammerhead ribozyme-mediated cleavage of the long terminal repeat RNA of human immunodeficiency virus type 1. J Biol Chem (1992) 1.45
The importance of being ribose at the cleavage site in the Tetrahymena ribozyme reaction. Biochemistry (1993) 1.45
Investigation of enzyme mechanisms with nucleoside phosphorothioates. Angew Chem Int Ed Engl (1975) 1.44
A study of the mechanism of T4 DNA polymerase with diastereomeric phosphorothioate analogues of deoxyadenosine triphosphate. J Biol Chem (1982) 1.44
Contributions of 2'-hydroxyl groups of the RNA substrate to binding and catalysis by the Tetrahymena ribozyme. An energetic picture of an active site composed of RNA. Biochemistry (1993) 1.44
Chiral phosphorothioate analogues of B-DNA. The crystal structure of Rp-d[Gp(S)CpGp(S)CpGp(S)C]. J Mol Biol (1986) 1.43
Filter disc supported oligonucleotide synthesis by the phosphite method. Nucleic Acids Res (1984) 1.43
2'-Fluoro- and 2'-amino-2'-deoxynucleoside 5'-triphosphates as substrates for T7 RNA polymerase. Biochemistry (1992) 1.41
Function of specific 2'-hydroxyl groups of guanosines in a hammerhead ribozyme probed by 2' modifications. Proc Natl Acad Sci U S A (1992) 1.41
Stereochemical course of DNA hydrolysis by nuclease S1. J Biol Chem (1983) 1.41
The osteoporotic vertebral structure is well adapted to the loads of daily life, but not to infrequent "error" loads. Bone (2004) 1.41
In situ measurement of articular cartilage deformation in intact femoropatellar joints under static loading. J Biomech (1999) 1.40
Definition of osteoarthritis on MRI: results of a Delphi exercise. Osteoarthritis Cartilage (2011) 1.40
Mg(2+)-dependent conformational changes in the hammerhead ribozyme. Biochemistry (1996) 1.40
Change in regional cartilage morphology and joint space width in osteoarthritis participants versus healthy controls: a multicentre study using 3.0 Tesla MRI and Lyon-Schuss radiography. Ann Rheum Dis (2010) 1.38
A single methyl group prevents the mischarging of a tRNA. Nat Struct Biol (1994) 1.37
Generation of left-handed Z-DNA in solution and visualization in polytene chromosomes by immunofluorescence. Cold Spring Harb Symp Quant Biol (1983) 1.36
Gender differences in knee joint cartilage thickness, volume and articular surface areas: assessment with quantitative three-dimensional MR imaging. Skeletal Radiol (2001) 1.35
Determination of 3D cartilage thickness data from MR imaging: computational method and reproducibility in the living. Magn Reson Med (1999) 1.34
The stereochemical course of the restriction endonuclease EcoRI-catalyzed reaction. J Biol Chem (1984) 1.33
Interobserver reproducibility of quantitative cartilage measurements: comparison of B-spline snakes and manual segmentation. Magn Reson Imaging (1999) 1.33
Effects of functional group changes in the EcoRI recognition site on the cleavage reaction catalyzed by the endonuclease. Biochemistry (1987) 1.32
Mechanism of ribose 2'-group discrimination by an RNA polymerase. Biochemistry (1997) 1.32
Crystal and molecular structure of adenosine 5'-O-phosphorothioate O-p-nitrophenyl ester (Sp diastereomer). Substrate stereospecificity of snake venom phosphodiesterase. Eur J Biochem (1979) 1.29
Stereochemistry of the guanyl nucleotide binding site of transducin probed by phosphorothioate analogues of GTP and GDP. Biochemistry (1985) 1.29
Premorbid knee osteoarthritis is not characterised by diffuse thinness: the Framingham Osteoarthritis Study. Ann Rheum Dis (2008) 1.27
Polynucleotides containing 2'-amino-2'-deoxyribose and 2'-azido-2'-deoxyribose. Biochemistry (1973) 1.27
Left-handed helical conformation of poly[d(A-m5C).d(G-T)]. Nature (1983) 1.27
Inter-domain cross-linking and molecular modelling of the hairpin ribozyme. J Mol Biol (1997) 1.25
Knee cartilage of spinal cord-injured patients displays progressive thinning in the absence of normal joint loading and movement. Arthritis Rheum (2002) 1.25
Subregional femorotibial cartilage morphology in women--comparison between healthy controls and participants with different grades of radiographic knee osteoarthritis. Osteoarthritis Cartilage (2009) 1.24
Feasibility of T and Z scores from magnetic resonance imaging data for quantification of cartilage loss in osteoarthritis. Arthritis Rheum (2003) 1.24
The association of prevalent medial meniscal pathology with cartilage loss in the medial tibiofemoral compartment over a 2-year period. Osteoarthritis Cartilage (2009) 1.22
Women have thinner cartilage and smaller joint surfaces than men after adjustment for body height and weight. Osteoarthritis Cartilage (2007) 1.21
Inhibition of deoxyribonucleases by phosphorothioate groups in oligodeoxyribonucleotides. Nucleic Acids Res (1988) 1.21
Chemically modified RNA: approaches and applications. FASEB J (1993) 1.20
Probing accessible sites for ribozymes on human acetylcholinesterase RNA. RNA (1997) 1.20
Sensitivity to change of cartilage morphometry using coronal FLASH, sagittal DESS, and coronal MPR DESS protocols--comparative data from the Osteoarthritis Initiative (OAI). Osteoarthritis Cartilage (2009) 1.19
Synthesis of ATP- and dATP-substituted sepharoses and their application in the purification of phage-T4-induced ribonucleotide reductase. Eur J Biochem (1972) 1.19
Polyribonucleotides containing a phosphorothioate backbone. Eur J Biochem (1970) 1.19
Longitudinal analysis of cartilage atrophy in the knees of patients with spinal cord injury. Arthritis Rheum (2003) 1.17
Probing RNA tertiary structure: interhelical crosslinking of the hammerhead ribozyme. RNA (1995) 1.17
MRI-based extended ordered values more efficiently differentiate cartilage loss in knees with and without joint space narrowing than region-specific approaches using MRI or radiography--data from the OA initiative. Osteoarthritis Cartilage (2011) 1.17