Published in J Biomol NMR on May 16, 2009
An introduction to NMR-based approaches for measuring protein dynamics. Biochim Biophys Acta (2010) 1.75
Chemical exchange in biomacromolecules: past, present, and future. J Magn Reson (2014) 1.24
Exploring sparsely populated states of macromolecules by diamagnetic and paramagnetic NMR relaxation. Protein Sci (2011) 1.04
Isotope labeling methods for studies of excited protein states by relaxation dispersion NMR spectroscopy. Nat Protoc (2009) 0.93
Probing microsecond time scale dynamics in proteins by methyl (1)H Carr-Purcell-Meiboom-Gill relaxation dispersion NMR measurements. Application to activation of the signaling protein NtrC(r). J Am Chem Soc (2010) 0.93
A simple method for measuring signs of (1)H (N) chemical shift differences between ground and excited protein states. J Biomol NMR (2010) 0.92
Quantifying millisecond time-scale exchange in proteins by CPMG relaxation dispersion NMR spectroscopy of side-chain carbonyl groups. J Biomol NMR (2011) 0.90
Conformational exchange of aromatic side chains characterized by L-optimized TROSY-selected ¹³C CPMG relaxation dispersion. J Biomol NMR (2012) 0.87
An exact solution for R2,eff in CPMG experiments in the case of two site chemical exchange. J Magn Reson (2014) 0.83
Specific 12CβD(2)12CγD(2)S13CεHD(2) isotopomer labeling of methionine to characterize protein dynamics by 1H and 13C NMR relaxation dispersion. J Am Chem Soc (2012) 0.82
Measurement of signs of chemical shift differences between ground and excited protein states: a comparison between H(S/M)QC and R1rho methods. J Biomol NMR (2009) 0.82
Measurement of the signs of methyl 13C chemical shift differences between interconverting ground and excited protein states by R(1ρ): an application to αB-crystallin. J Biomol NMR (2012) 0.81
Site-selective (13)C labeling of proteins using erythrose. J Biomol NMR (2017) 0.78
Measuring 1HN temperature coefficients in invisible protein states by relaxation dispersion NMR spectroscopy. J Biomol NMR (2011) 0.78
Fractional enrichment of proteins using [2-(13)C]-glycerol as the carbon source facilitates measurement of excited state 13Cα chemical shifts with improved sensitivity. J Biomol NMR (2015) 0.77
Off-resonance rotating-frame relaxation dispersion experiment for 13C in aromatic side chains using L-optimized TROSY-selection. J Biomol NMR (2014) 0.77
Protein conformational exchange measured by 1H R1ρ relaxation dispersion of methyl groups. J Biomol NMR (2013) 0.76
Probing conformational dynamics in biomolecules via chemical exchange saturation transfer: a primer. J Biomol NMR (2017) 0.75
Measurement of protein backbone (13)CO and (15)N relaxation dispersion at high resolution. J Biomol NMR (2017) 0.75
(13)C-NMR studies on disulfide bond isomerization in bovine pancreatic trypsin inhibitor (BPTI). J Biomol NMR (2016) 0.75
Site-selective (13)C labeling of histidine and tryptophan using ribose. J Biomol NMR (2017) 0.75
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A (2000) 96.90
NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR (1995) 93.94
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol (1986) 55.34
Protein backbone angle restraints from searching a database for chemical shift and sequence homology. J Biomol NMR (1999) 26.99
The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data. J Biomol NMR (1994) 16.20
Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease. Biochemistry (1989) 10.50
The dynamic energy landscape of dihydrofolate reductase catalysis. Science (2006) 7.55
Intrinsic dynamics of an enzyme underlies catalysis. Nature (2005) 7.32
Mechanism of coupled folding and binding of an intrinsically disordered protein. Nature (2007) 6.26
Nuclear magnetic resonance methods for quantifying microsecond-to-millisecond motions in biological macromolecules. Methods Enzymol (2001) 5.58
Amyloid fibrils of the HET-s(218-289) prion form a beta solenoid with a triangular hydrophobic core. Science (2008) 5.55
Enzyme dynamics during catalysis. Science (2002) 4.39
Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy. Nature (2002) 4.29
Studying excited states of proteins by NMR spectroscopy. Nat Struct Biol (2001) 4.07
Homology of a yeast actin-binding protein to signal transduction proteins and myosin-I. Nature (1990) 3.90
Linkage between dynamics and catalysis in a thermophilic-mesophilic enzyme pair. Nat Struct Mol Biol (2004) 3.41
Protein backbone chemical shifts predicted from searching a database for torsion angle and sequence homology. J Biomol NMR (2007) 3.20
Slow dynamics in folded and unfolded states of an SH3 domain. J Am Chem Soc (2001) 3.14
A robust and cost-effective method for the production of Val, Leu, Ile (delta 1) methyl-protonated 15N-, 13C-, 2H-labeled proteins. J Biomol NMR (1999) 3.02
Use of chemical shifts in macromolecular structure determination. Methods Enzymol (2001) 2.91
Optimal isotope labelling for NMR protein structure determinations. Nature (2006) 2.89
Low-populated folding intermediates of Fyn SH3 characterized by relaxation dispersion NMR. Nature (2004) 2.84
The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins. Annu Rev Biophys Biomol Struct (1998) 2.76
Three-dimensional triple-resonance NMR Spectroscopy of isotopically enriched proteins. 1990. J Magn Reson (2011) 2.58
Four-dimensional heteronuclear triple-resonance NMR spectroscopy of interleukin-1 beta in solution. Science (1990) 2.28
Rotational diffusion anisotropy of proteins from simultaneous analysis of 15N and 13C alpha nuclear spin relaxation. J Biomol NMR (1997) 2.18
Evidence for physical and functional interactions among two Saccharomyces cerevisiae SH3 domain proteins, an adenylyl cyclase-associated protein and the actin cytoskeleton. Mol Biol Cell (1997) 1.94
Measurement of slow (micros-ms) time scale dynamics in protein side chains by (15)N relaxation dispersion NMR spectroscopy: application to Asn and Gln residues in a cavity mutant of T4 lysozyme. J Am Chem Soc (2001) 1.91
The mechanism of rate-limiting motions in enzyme function. Proc Natl Acad Sci U S A (2007) 1.67
Reconstructing NMR spectra of "invisible" excited protein states using HSQC and HMQC experiments. J Am Chem Soc (2002) 1.66
Probing chemical shifts of invisible states of proteins with relaxation dispersion NMR spectroscopy: how well can we do? J Am Chem Soc (2008) 1.64
Measurement of bond vector orientations in invisible excited states of proteins. Proc Natl Acad Sci U S A (2007) 1.52
Fractional 13C enrichment of isolated carbons using [1-13C]- or [2- 13C]-glucose facilitates the accurate measurement of dynamics at backbone Calpha and side-chain methyl positions in proteins. J Biomol NMR (2007) 1.49
Structures of invisible, excited protein states by relaxation dispersion NMR spectroscopy. Proc Natl Acad Sci U S A (2008) 1.49
An isotope labeling strategy for methyl TROSY spectroscopy. J Biomol NMR (2004) 1.41
Slow internal dynamics in proteins: application of NMR relaxation dispersion spectroscopy to methyl groups in a cavity mutant of T4 lysozyme. J Am Chem Soc (2002) 1.39
Multiple-site exchange in proteins studied with a suite of six NMR relaxation dispersion experiments: an application to the folding of a Fyn SH3 domain mutant. J Am Chem Soc (2005) 1.31
Extending the range of amide proton relaxation dispersion experiments in proteins using a constant-time relaxation-compensated CPMG approach. J Biomol NMR (2003) 1.28
A single-quantum methyl 13C-relaxation dispersion experiment with improved sensitivity. J Biomol NMR (2007) 1.27
Solution NMR spin relaxation methods for characterizing chemical exchange in high-molecular-weight systems. Methods Enzymol (2005) 1.19
The FLP protein of the 2-micron plasmid of yeast. Inter- and intramolecular reactions. J Biol Chem (1985) 1.17
A sensitive and robust method for obtaining intermolecular NOEs between side chains in large protein complexes. J Biomol NMR (2003) 1.16
Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum. Eur J Biochem (1998) 1.14
Carbon relaxation in randomly fractionally 13C-enriched proteins. J Magn Reson B (1995) 1.12
Characterization of chemical exchange using residual dipolar coupling. J Am Chem Soc (2007) 1.07
Measurement of carbonyl chemical shifts of excited protein states by relaxation dispersion NMR spectroscopy: comparison between uniformly and selectively (13)C labeled samples. J Biomol NMR (2008) 1.06
Probing structure in invisible protein states with anisotropic NMR chemical shifts. J Am Chem Soc (2008) 1.06
The biologically relevant targets and binding affinity requirements for the function of the yeast actin-binding protein 1 Src-homology 3 domain vary with genetic context. Genetics (2007) 1.05
Accurate measurement of alpha proton chemical shifts of excited protein states by relaxation dispersion NMR spectroscopy. J Am Chem Soc (2009) 1.01
Complementarity of ensemble and single-molecule measures of protein motion: a relaxation dispersion NMR study of an enzyme complex. Proc Natl Acad Sci U S A (2006) 1.00
Carbonyl carbon transverse relaxation dispersion measurements and ms-micros timescale motion in a protein hydrogen bond network. J Biomol NMR (2004) 0.98
NMR spectroscopic characterization of millisecond protein folding by transverse relaxation dispersion measurements. J Am Chem Soc (2005) 0.94
The design of a hyperstable mutant of the Abp1p SH3 domain by sequence alignment analysis. Protein Sci (2000) 0.93
Quantifying two-bond 1HN-13CO and one-bond 1H(alpha)-13C(alpha) dipolar couplings of invisible protein states by spin-state selective relaxation dispersion NMR spectroscopy. J Am Chem Soc (2008) 0.92
Deuterium labelling in NMR structural analysis of larger proteins. Q Rev Biophys (1990) 0.92
CPMG relaxation dispersion NMR experiments measuring glycine 1H alpha and 13C alpha chemical shifts in the 'invisible' excited states of proteins. J Biomol NMR (2009) 0.89
Intrinsic dynamics of an enzyme underlies catalysis. Nature (2005) 7.32
New tools provide new insights in NMR studies of protein dynamics. Science (2006) 4.64
Tissue Doppler echocardiographic evidence of reverse remodeling and improved synchronicity by simultaneously delaying regional contraction after biventricular pacing therapy in heart failure. Circulation (2002) 4.02
Quantitative dynamics and binding studies of the 20S proteasome by NMR. Nature (2007) 3.63
Complete genome sequence of citrus huanglongbing bacterium, 'Candidatus Liberibacter asiaticus' obtained through metagenomics. Mol Plant Microbe Interact (2009) 3.33
Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy. Nat Protoc (2006) 3.14
Tissue Doppler imaging is superior to strain rate imaging and postsystolic shortening on the prediction of reverse remodeling in both ischemic and nonischemic heart failure after cardiac resynchronization therapy. Circulation (2004) 3.12
A transient and low-populated protein-folding intermediate at atomic resolution. Science (2010) 3.08
NMR spectroscopy brings invisible protein states into focus. Nat Chem Biol (2009) 3.00
Cross-correlated relaxation enhanced 1H[bond]13C NMR spectroscopy of methyl groups in very high molecular weight proteins and protein complexes. J Am Chem Soc (2003) 2.99
Low-populated folding intermediates of Fyn SH3 characterized by relaxation dispersion NMR. Nature (2004) 2.84
Three-dimensional triple-resonance NMR Spectroscopy of isotopically enriched proteins. 1990. J Magn Reson (2011) 2.58
Predictors of left ventricular reverse remodeling after cardiac resynchronization therapy for heart failure secondary to idiopathic dilated or ischemic cardiomyopathy. Am J Cardiol (2003) 2.53
Solution structure of a minor and transiently formed state of a T4 lysozyme mutant. Nature (2011) 2.49
Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region. Science (2005) 2.47
Structural coupling of SH2-kinase domains links Fes and Abl substrate recognition and kinase activation. Cell (2008) 2.46
Solution structure and dynamics of the outer membrane enzyme PagP by NMR. Proc Natl Acad Sci U S A (2002) 2.39
Structure/function implications in a dynamic complex of the intrinsically disordered Sic1 with the Cdc4 subunit of an SCF ubiquitin ligase. Structure (2010) 2.33
Protein dynamics and conformational disorder in molecular recognition. J Mol Recognit (2010) 2.31
PyK2 and FAK connections to p190Rho guanine nucleotide exchange factor regulate RhoA activity, focal adhesion formation, and cell motility. J Cell Biol (2008) 2.31
Structure of an intermediate state in protein folding and aggregation. Science (2012) 2.24
Ile, Leu, and Val methyl assignments of the 723-residue malate synthase G using a new labeling strategy and novel NMR methods. J Am Chem Soc (2003) 2.20
Observing biological dynamics at atomic resolution using NMR. Trends Biochem Sci (2009) 2.19
Unraveling the mechanism of protein disaggregation through a ClpB-DnaK interaction. Science (2013) 2.17
Probing invisible, low-populated States of protein molecules by relaxation dispersion NMR spectroscopy: an application to protein folding. Acc Chem Res (2008) 2.15
Progression of systolic abnormalities in patients with "isolated" diastolic heart failure and diastolic dysfunction. Circulation (2002) 2.09
A novel tool to assess systolic asynchrony and identify responders of cardiac resynchronization therapy by tissue synchronization imaging. J Am Coll Cardiol (2005) 2.03
Refined solution structure of the 82-kDa enzyme malate synthase G from joint NMR and synchrotron SAXS restraints. J Biomol NMR (2007) 2.00
Dynamic equilibrium engagement of a polyvalent ligand with a single-site receptor. Proc Natl Acad Sci U S A (2008) 2.00
Bone loss caused by iron overload in a murine model: importance of oxidative stress. Blood (2010) 1.98
Solution NMR-derived global fold of a monomeric 82-kDa enzyme. Proc Natl Acad Sci U S A (2005) 1.92
Nuclear magnetic resonance spectroscopy of high-molecular-weight proteins. Annu Rev Biochem (2004) 1.84
Influenza-associated mortality in temperate and subtropical Chinese cities, 2003-2008. Bull World Health Organ (2012) 1.69
Dynamic regulation of archaeal proteasome gate opening as studied by TROSY NMR. Science (2010) 1.69
Quantitative NMR spectroscopy of supramolecular complexes: dynamic side pores in ClpP are important for product release. Proc Natl Acad Sci U S A (2005) 1.68
Direct interaction of focal adhesion kinase with p190RhoGEF. J Biol Chem (2003) 1.67
Reconstructing NMR spectra of "invisible" excited protein states using HSQC and HMQC experiments. J Am Chem Soc (2002) 1.66
Probing slow dynamics in high molecular weight proteins by methyl-TROSY NMR spectroscopy: application to a 723-residue enzyme. J Am Chem Soc (2004) 1.65
Probing chemical shifts of invisible states of proteins with relaxation dispersion NMR spectroscopy: how well can we do? J Am Chem Soc (2008) 1.64
Novel proteasome inhibitors to overcome bortezomib resistance. J Natl Cancer Inst (2011) 1.63
Quaternary dynamics and plasticity underlie small heat shock protein chaperone function. Proc Natl Acad Sci U S A (2010) 1.63
A hydrocarbon ruler measures palmitate in the enzymatic acylation of endotoxin. EMBO J (2004) 1.60
Architecture of the high mobility group nucleosomal protein 2-nucleosome complex as revealed by methyl-based NMR. Proc Natl Acad Sci U S A (2011) 1.59
Measurement of bond vector orientations in invisible excited states of proteins. Proc Natl Acad Sci U S A (2007) 1.52
The energetic cost of domain reorientation in maltose-binding protein as studied by NMR and fluorescence spectroscopy. Proc Natl Acad Sci U S A (2003) 1.51
High-resolution four-dimensional 1H-13C NOE spectroscopy using methyl-TROSY, sparse data acquisition, and multidimensional decomposition. J Am Chem Soc (2005) 1.50
Deuterium spin probes of side-chain dynamics in proteins. 1. Measurement of five relaxation rates per deuteron in (13)C-labeled and fractionally (2)H-enriched proteins in solution. J Am Chem Soc (2002) 1.50
Confirmation of gene expression-based prediction of survival in non-small cell lung cancer. Clin Cancer Res (2008) 1.49
Fractional 13C enrichment of isolated carbons using [1-13C]- or [2- 13C]-glucose facilitates the accurate measurement of dynamics at backbone Calpha and side-chain methyl positions in proteins. J Biomol NMR (2007) 1.49
Structures of invisible, excited protein states by relaxation dispersion NMR spectroscopy. Proc Natl Acad Sci U S A (2008) 1.49
Breast cancer subpopulation with high risk of internal mammary lymph nodes metastasis: analysis of 2,269 Chinese breast cancer patients treated with extended radical mastectomy. Breast Cancer Res Treat (2007) 1.49
Four-dimensional NMR spectroscopy of a 723-residue protein: chemical shift assignments and secondary structure of malate synthase g. J Am Chem Soc (2002) 1.49
NMR paves the way for atomic level descriptions of sparsely populated, transiently formed biomolecular conformers. Proc Natl Acad Sci U S A (2013) 1.48
αB-crystallin polydispersity is a consequence of unbiased quaternary dynamics. J Mol Biol (2011) 1.48
Methyl groups as probes of structure and dynamics in NMR studies of high-molecular-weight proteins. Chembiochem (2005) 1.48
A solution NMR study showing that active site ligands and nucleotides directly perturb the allosteric equilibrium in aspartate transcarbamoylase. Proc Natl Acad Sci U S A (2007) 1.46
NMR studies of protein structure and dynamics. 2005. J Magn Reson (2011) 1.46
Methyl groups as probes of supra-molecular structure, dynamics and function. J Biomol NMR (2009) 1.43
An isotope labeling strategy for methyl TROSY spectroscopy. J Biomol NMR (2004) 1.41
Distribution of molecular size within an unfolded state ensemble using small-angle X-ray scattering and pulse field gradient NMR techniques. J Mol Biol (2002) 1.41
Studying "invisible" excited protein states in slow exchange with a major state conformation. J Am Chem Soc (2012) 1.40
Bilateral stereotactic radiofrequency amygdalohippocampectomy for a patient with bilateral temporal lobe epilepsy. Epilepsia (2013) 1.40
NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B. Nat Struct Mol Biol (2008) 1.40
Quaternary dynamics of αB-crystallin as a direct consequence of localised tertiary fluctuations in the C-terminus. J Mol Biol (2011) 1.39
Slow internal dynamics in proteins: application of NMR relaxation dispersion spectroscopy to methyl groups in a cavity mutant of T4 lysozyme. J Am Chem Soc (2002) 1.39
Poly[dimethyl-ammonium [aquadi-μ(2)-oxalato-dysprosate(III)] trihydrate]. Acta Crystallogr Sect E Struct Rep Online (2010) 1.38
The proteasome antechamber maintains substrates in an unfolded state. Nature (2010) 1.34
Quantitative NMR studies of high molecular weight proteins: application to domain orientation and ligand binding in the 723 residue enzyme malate synthase G. J Mol Biol (2003) 1.33
Multiple-site exchange in proteins studied with a suite of six NMR relaxation dispersion experiments: an application to the folding of a Fyn SH3 domain mutant. J Am Chem Soc (2005) 1.31
Solution NMR of supramolecular complexes: providing new insights into function. Nat Methods (2007) 1.30
A change in conformational dynamics underlies the activation of Eph receptor tyrosine kinases. EMBO J (2006) 1.30
Off-resonance R(1rho) NMR studies of exchange dynamics in proteins with low spin-lock fields: an application to a Fyn SH3 domain. J Am Chem Soc (2005) 1.29
An improved 15N relaxation dispersion experiment for the measurement of millisecond time-scale dynamics in proteins. J Phys Chem B (2007) 1.28
A single-quantum methyl 13C-relaxation dispersion experiment with improved sensitivity. J Biomol NMR (2007) 1.27
Identification of a collapsed intermediate with non-native long-range interactions on the folding pathway of a pair of Fyn SH3 domain mutants by NMR relaxation dispersion spectroscopy. J Mol Biol (2006) 1.26
Structure of a regulatory complex involving the Abl SH3 domain, the Crk SH2 domain, and a Crk-derived phosphopeptide. Proc Natl Acad Sci U S A (2002) 1.26
An NMR experiment for the accurate measurement of heteronuclear spin-lock relaxation rates. J Am Chem Soc (2002) 1.25
The integral membrane enzyme PagP alternates between two dynamically distinct states. Proc Natl Acad Sci U S A (2004) 1.25
Using relaxation dispersion NMR spectroscopy to determine structures of excited, invisible protein states. J Biomol NMR (2008) 1.23
Relative overexpression of X-linked genes in mouse embryonic stem cells is consistent with Ohno's hypothesis. Nat Genet (2011) 1.22
A PKC epsilon-ENH-channel complex specifically modulates N-type Ca2+ channels. Nat Neurosci (2003) 1.21
Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family. Cell Res (2006) 1.20
Dramatic acceleration of protein folding by stabilization of a nonnative backbone conformation. Proc Natl Acad Sci U S A (2004) 1.19
The polydispersity of αB-crystallin is rationalized by an interconverting polyhedral architecture. Structure (2011) 1.15
A "three-pronged" binding mechanism for the SAP/SH2D1A SH2 domain: structural basis and relevance to the XLP syndrome. EMBO J (2002) 1.15
The repair of large segmental bone defects in the rabbit with vascularized tissue engineered bone. Biomaterials (2009) 1.13
LMP1 and LMP2A are potential prognostic markers of extranodal NK/T-cell lymphoma, nasal type (ENKTL). Diagn Pathol (2012) 1.12
An exchange-free measure of 15N transverse relaxation: an NMR spectroscopy application to the study of a folding intermediate with pervasive chemical exchange. J Am Chem Soc (2007) 1.11
Determination of isoleucine side-chain conformations in ground and excited states of proteins from chemical shifts. J Am Chem Soc (2010) 1.11
Probing side-chain dynamics in the proteasome by relaxation violated coherence transfer NMR spectroscopy. J Am Chem Soc (2007) 1.10
MMP-2 mediates ethanol-induced invasion of mammary epithelial cells over-expressing ErbB2. Int J Cancer (2006) 1.09
Proteasome allostery as a population shift between interchanging conformers. Proc Natl Acad Sci U S A (2012) 1.09
In support of the BMRB. Nat Struct Mol Biol (2012) 1.09
Site-specific contributions to the pH dependence of protein stability. Proc Natl Acad Sci U S A (2003) 1.09
Effect of noncompetitive proteasome inhibition on bortezomib resistance. J Natl Cancer Inst (2010) 1.09
Diversity and plasticity of the intracellular plant pathogen and insect symbiont "Candidatus Liberibacter asiaticus" as revealed by hypervariable prophage genes with intragenic tandem repeats. Appl Environ Microbiol (2011) 1.09
Explorations in organic chemistry leading to the total synthesis of (+/-)-gelsemine. J Am Chem Soc (2002) 1.09
Deuterium spin probes of side-chain dynamics in proteins. 2. Spectral density mapping and identification of nanosecond time-scale side-chain motions. J Am Chem Soc (2002) 1.08