Utility of a commercially available multiplex real-time PCR assay to detect bacterial and fungal pathogens in febrile neutropenia.

PubWeight™: 1.59‹?› | Rank: Top 4%

🔗 View Article (PMC 2725651)

Published in J Clin Microbiol on July 01, 2009

Authors

Marie von Lilienfeld-Toal1, Lutz E Lehmann, Ansgar D Raadts, Corinna Hahn-Ast, Katjana S Orlopp, Günter Marklein, Ingvill Purr, Gordon Cook, Andreas Hoeft, Axel Glasmacher, Frank Stüber

Author Affiliations

1: Med. Klinik und Poliklinik III, Universitätsklinikum Bonn, Germany. m.lilienfeld.toal@uni-bonn.de

Articles citing this

Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol (2009) 3.54

The era of molecular and other non-culture-based methods in diagnosis of sepsis. Clin Microbiol Rev (2010) 2.90

New approaches to sepsis: molecular diagnostics and biomarkers. Clin Microbiol Rev (2012) 2.01

Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis - a systemic review and meta-analysis. PLoS One (2013) 1.94

Multiplex PCR to diagnose bloodstream infections in patients admitted from the emergency department with sepsis. J Clin Microbiol (2009) 1.63

Molecular probes for diagnosis of clinically relevant bacterial infections in blood cultures. J Clin Microbiol (2010) 1.61

PCR diagnosis of invasive candidiasis: systematic review and meta-analysis. J Clin Microbiol (2010) 1.59

Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia. J Clin Microbiol (2010) 1.38

Molecular diagnosis of sepsis: New aspects and recent developments. Eur J Microbiol Immunol (Bp) (2014) 1.30

Laboratory diagnosis of invasive aspergillosis: from diagnosis to prediction of outcome. Scientifica (Cairo) (2013) 1.14

Evaluation of a polymerase chain reaction assay for pathogen detection in septic patients under routine condition: an observational study. PLoS One (2012) 1.12

Evaluation of a commercial multiplex PCR test (SeptiFast) in the etiological diagnosis of community-onset bloodstream infections. Eur J Clin Microbiol Infect Dis (2011) 1.08

Detection, identification, and distribution of fungi in bronchoalveolar lavage specimens by use of multilocus PCR coupled with electrospray ionization/mass spectrometry. J Clin Microbiol (2012) 1.07

Highly sensitive detection of Staphylococcus aureus directly from patient blood. PLoS One (2012) 0.98

A real-time PCR antibiogram for drug-resistant sepsis. PLoS One (2011) 0.95

Rapid antimicrobial susceptibility testing with electrokinetics enhanced biosensors for diagnosis of acute bacterial infections. Ann Biomed Eng (2014) 0.94

Polymerase chain reaction-based assays for the diagnosis of invasive fungal infections. Clin Infect Dis (2012) 0.93

Multiplex PCR for rapid and improved diagnosis of bloodstream infections in liver transplant recipients. J Clin Microbiol (2012) 0.90

Diagnostic value of PCR analysis of bacteria and fungi from blood in empiric-therapy-resistant febrile neutropenia. J Clin Microbiol (2010) 0.88

Impact of multiplex PCR on antimicrobial treatment in febrile neutropenia: a randomized controlled study. Med Microbiol Immunol (2015) 0.88

Management of sepsis in neutropenic patients: 2014 updated guidelines from the Infectious Diseases Working Party of the German Society of Hematology and Medical Oncology (AGIHO). Ann Hematol (2014) 0.84

A Broad-Spectrum Infection Diagnostic that Detects Pathogen-Associated Molecular Patterns (PAMPs) in Whole Blood. EBioMedicine (2016) 0.84

Performance of the LightCycler SeptiFast test Mgrade in detecting microbial pathogens in purulent fluids. J Clin Microbiol (2011) 0.83

Multiplex PCR performed of bronchoalveolar lavage fluid increases pathogen identification rate in critically ill patients with pneumonia: a pilot study. Ann Intensive Care (2014) 0.83

Infections Caused by Stenotrophomonas maltophilia in Recipients of Hematopoietic Stem Cell Transplantation. Front Oncol (2014) 0.83

Development and evaluation of oligonucleotide chip based on the 16S-23S rRNA gene spacer region for detection of pathogenic microorganisms associated with sepsis. J Clin Microbiol (2010) 0.79

Rapid separation of bacteria from blood-review and outlook. Biotechnol Prog (2016) 0.79

Diagnosis of bacteremia in pediatric oncologic patients by in-house real-time PCR. BMC Infect Dis (2015) 0.78

A novel, multiplex, real-time PCR-based approach for the detection of the commonly occurring pathogenic fungi and bacteria. BMC Microbiol (2013) 0.76

Broad-Range PCR Coupled with Electrospray Ionization Time of Flight Mass Spectrometry for Detection of Bacteremia and Fungemia in Patients with Neutropenic Fever. J Clin Microbiol (2016) 0.75

Polymerase Chain Reaction molecular diagnostic technology for monitoring chronic osteomyelitis. J Exp Orthop (2014) 0.75

Diagnosis and empirical treatment of fever of unknown origin (FUO) in adult neutropenic patients: guidelines of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Medical Oncology (DGHO). Ann Hematol (2017) 0.75

Articles cited by this

Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: an international consensus. Clin Infect Dis (2001) 12.73

2002 guidelines for the use of antimicrobial agents in neutropenic patients with cancer. Clin Infect Dis (2002) 7.81

A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol (2007) 2.64

Aspergillus galactomannan detection in the diagnosis of invasive aspergillosis in cancer patients. J Clin Oncol (2002) 2.31

Immune cell activation by bacterial CpG-DNA through myeloid differentiation marker 88 and tumor necrosis factor receptor-associated factor (TRAF)6. J Exp Med (2000) 2.16

Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies. J Med Microbiol (2008) 2.02

Detection of bacteraemia in patients with fever and neutropenia using 16S rRNA gene amplification by polymerase chain reaction. Eur J Clin Microbiol Infect Dis (1998) 1.78

Procalcitonin in paediatric cancer patients: its diagnostic relevance is superior to that of C-reactive protein, interleukin 6, interleukin 8, soluble interleukin 2 receptor and soluble tumour necrosis factor receptor II. Br J Haematol (2000) 1.53

Usefulness of blood culture for hospitalized patients who are receiving antibiotic therapy. Clin Infect Dis (2001) 1.20

Use of quantitative 16S ribosomal DNA detection for diagnosis of central vascular catheter-associated bacterial infection. J Clin Microbiol (2004) 1.18

Markers of bacteremia in febrile neutropenic patients with hematological malignancies: procalcitonin and IL-6 are more reliable than C-reactive protein. Eur J Clin Microbiol Infect Dis (2004) 1.18

Utility of obtaining blood cultures in febrile neutropenic patients undergoing bone marrow transplantation. Bone Marrow Transplant (2000) 1.12

Infections in neutropenic cancer patients. Lancet (2002) 0.95

Detection of bacterial DNA in blood samples from febrile patients: underestimated infection or emerging contamination? FEMS Immunol Med Microbiol (2004) 0.95

Diagnosis and antimicrobial therapy of pulmonary infiltrates in febrile neutropenic patients--guidelines of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Oncology (DGHO). Ann Hematol (2003) 0.90

Real-time broad-range PCR versus blood culture. A prospective pilot study in pediatric cancer patients with fever and neutropenia. Support Care Cancer (2007) 0.86

False positivity of the Aspergillus galactomannan Platelia ELISA because of piperacillin/tazobactam treatment: does it represent a clinical problem? J Antimicrob Chemother (2008) 0.86

Articles by these authors

Mortality after surgery in Europe: a 7 day cohort study. Lancet (2012) 9.96

Complications of the COX-2 inhibitors parecoxib and valdecoxib after cardiac surgery. N Engl J Med (2005) 6.32

Mortality associated with aprotinin during 5 years following coronary artery bypass graft surgery. JAMA (2007) 6.04

First-line treatment with zoledronic acid as compared with clodronic acid in multiple myeloma (MRC Myeloma IX): a randomised controlled trial. Lancet (2010) 5.04

Minimal residual disease assessed by multiparameter flow cytometry in multiple myeloma: impact on outcome in the Medical Research Council Myeloma IX Study. J Clin Oncol (2013) 3.75

European Society of Anaesthesiology Clinical Trials Network. Eur J Anaesthesiol (2011) 3.36

Prospective external validation of a predictive score for postoperative pulmonary complications. Anesthesiology (2014) 3.01

Lack of effectiveness of the pulmonary artery catheter in cardiac surgery. Anesth Analg (2011) 2.83

The role of maintenance thalidomide therapy in multiple myeloma: MRC Myeloma IX results and meta-analysis. Blood (2011) 2.69

A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol (2007) 2.64

Hemodynamic monitoring and management in patients undergoing high risk surgery: a survey among North American and European anesthesiologists. Crit Care (2011) 2.59

IgM myeloma: a rare entity characterized by a CD20-CD56-CD117- immunophenotype and the t(11;14). Br J Haematol (2008) 2.54

Tandem versus single autologous hematopoietic cell transplantation for the treatment of multiple myeloma: a systematic review and meta-analysis. J Natl Cancer Inst (2009) 2.46

Azacitidine augments expansion of regulatory T cells after allogeneic stem cell transplantation in patients with acute myeloid leukemia (AML). Blood (2012) 2.39

Outcomes after alemtuzumab-containing reduced-intensity allogeneic transplantation regimen for relapsed and refractory non-Hodgkin lymphoma. Blood (2004) 2.31

Allogeneic stem-cell transplantation using a reduced-intensity conditioning regimen has the capacity to produce durable remissions and long-term disease-free survival in patients with high-risk acute myeloid leukemia and myelodysplasia. J Clin Oncol (2005) 2.20

Guidelines for the diagnosis and management of multiple myeloma 2011. Br J Haematol (2011) 2.14

Primary central nervous system lymphoma: results of a pilot and phase II study of systemic and intraventricular chemotherapy with deferred radiotherapy. J Clin Oncol (2003) 2.14

2014 ESC/ESA Guidelines on non-cardiac surgery: cardiovascular assessment and management: The Joint Task Force on non-cardiac surgery: cardiovascular assessment and management of the European Society of Cardiology (ESC) and the European Society of Anaesthesiology (ESA). Eur Heart J (2014) 2.10

T-cell-depleted reduced-intensity transplantation followed by donor leukocyte infusions to promote graft-versus-lymphoma activity results in excellent long-term survival in patients with multiply relapsed follicular lymphoma. J Clin Oncol (2010) 2.03

A survey of acute pain services in Germany and a discussion of international survey data. Reg Anesth Pain Med (2002) 2.02

Bacterial DNA induces myocardial inflammation and reduces cardiomyocyte contractility: role of toll-like receptor 9. Cardiovasc Res (2008) 1.80

Reduced intensity conditioning allogeneic stem cell transplantation for Hodgkin's lymphoma: identification of prognostic factors predicting outcome. Haematologica (2008) 1.80

Effects of zoledronic acid versus clodronic acid on skeletal morbidity in patients with newly diagnosed multiple myeloma (MRC Myeloma IX): secondary outcomes from a randomised controlled trial. Lancet Oncol (2011) 1.71

The requirement for DNAM-1, NKG2D, and NKp46 in the natural killer cell-mediated killing of myeloma cells. Cancer Res (2007) 1.70

Standards for definitions and use of outcome measures for clinical effectiveness research in perioperative medicine: European Perioperative Clinical Outcome (EPCO) definitions: a statement from the ESA-ESICM joint taskforce on perioperative outcome measures. Eur J Anaesthesiol (2015) 1.64

Favorable long-term survival after reduced-intensity allogeneic transplantation for multiple-relapse aggressive non-Hodgkin's lymphoma. J Clin Oncol (2008) 1.64

DHAP in combination with rituximab vs DHAP alone as salvage treatment for patients with relapsed or refractory diffuse large B-cell lymphoma: a matched-pair analysis. Leuk Lymphoma (2006) 1.61

2014 ESC/ESA Guidelines on non-cardiac surgery: cardiovascular assessment and management: The Joint Task Force on non-cardiac surgery: cardiovascular assessment and management of the European Society of Cardiology (ESC) and the European Society of Anaesthesiology (ESA). Eur J Anaesthesiol (2014) 1.59

Pseudomembranous and obstructive Aspergillus tracheobronchitis - optimal diagnostic strategy and outcome. Mycoses (2006) 1.58

Prospective evaluation of allogeneic hematopoietic stem-cell transplantation from matched related and matched unrelated donors in younger adults with high-risk acute myeloid leukemia: German-Austrian trial AMLHD98A. J Clin Oncol (2010) 1.57

Improved detection of blood stream pathogens by real-time PCR in severe sepsis. Intensive Care Med (2009) 1.51

Hospital-acquired legionellosis originating from a cooling tower during a period of thermal inversion. Int J Hyg Environ Health (2007) 1.48

Development and validation of a score to predict postoperative respiratory failure in a multicentre European cohort: A prospective, observational study. Eur J Anaesthesiol (2015) 1.47

Ultrasound confirmation of central venous catheter position via a right supraclavicular fossa view using a microconvex probe: an observational pilot study. Eur J Anaesthesiol (2015) 1.45

Evaluation of serum sandwich enzyme-linked immunosorbent assay for circulating galactomannan during caspofungin therapy: results from the caspofungin invasive aspergillosis study. Clin Infect Dis (2005) 1.43

Neutropenic enterocolitis in adults: systematic analysis of evidence quality. Eur J Haematol (2005) 1.42

Peristaltic pneumatic compression of the legs reduces fluid demand and improves hemodynamic stability during surgery: a randomized, prospective study. Anesthesiology (2011) 1.41

Etomidate for critically ill patients: still a matter for pro-con debates? Eur J Anaesthesiol (2014) 1.39

Toll-like receptor 4, nitric oxide, and myocardial depression in endotoxemia. Shock (2006) 1.39

Cost and mortality prediction using polymerase chain reaction pathogen detection in sepsis: evidence from three observational trials. Crit Care (2010) 1.33

Cyclophosphamide, thalidomide, and dexamethasone (CTD) as initial therapy for patients with multiple myeloma unsuitable for autologous transplantation. Blood (2011) 1.32

Cyclophosphamide, thalidomide, and dexamethasone as induction therapy for newly diagnosed multiple myeloma patients destined for autologous stem-cell transplantation: MRC Myeloma IX randomized trial results. Haematologica (2011) 1.32

Results of alemtuzumab-based reduced-intensity allogeneic transplantation for chronic lymphocytic leukemia: a British Society of Blood and Marrow Transplantation Study. Blood (2005) 1.31

Multicenter, noncomparative study of caspofungin in combination with other antifungals as salvage therapy in adults with invasive aspergillosis. Cancer (2006) 1.30

Potential clinical utility of polymerase chain reaction in microbiological testing for sepsis. Crit Care Med (2009) 1.29

Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR. Mol Biotechnol (2002) 1.26

Voriconazole versus itraconazole for antifungal prophylaxis following allogeneic haematopoietic stem-cell transplantation. Br J Haematol (2011) 1.25

The fate of human Langerhans cells in hematopoietic stem cell transplantation. J Exp Med (2006) 1.24

Induction of Bim and Bid gene expression during accelerated apoptosis in severe sepsis. Crit Care (2008) 1.21

Toll-like receptor 4 deficiency: smaller infarcts, but no gain in function. BMC Physiol (2007) 1.19

Impact of CYP2D6 genotype on postoperative tramadol analgesia. Pain (2003) 1.19

Factors predicting long-term survival after T-cell depleted reduced intensity allogeneic stem cell transplantation for acute myeloid leukemia. Haematologica (2009) 1.17

CD4(+)CD25(+)FoxP3(+) regulatory T cells are increased whilst CD3(+)CD4(-)CD8(-)alphabetaTCR(+) Double Negative T cells are decreased in the peripheral blood of patients with multiple myeloma which correlates with disease burden. Br J Haematol (2008) 1.17

A novel bispecific protein (ULBP2-BB4) targeting the NKG2D receptor on natural killer (NK) cells and CD138 activates NK cells and has potent antitumor activity against human multiple myeloma in vitro and in vivo. Blood (2005) 1.16

Bisphosphonates in multiple myeloma: a network meta-analysis. Cochrane Database Syst Rev (2012) 1.16

Persistent cognitive impairment, hippocampal atrophy and EEG changes in sepsis survivors. J Neurol Neurosurg Psychiatry (2012) 1.15

Clinical pharmacology of antifungal compounds. Infect Dis Clin North Am (2003) 1.14

Real-time PCR for detection and differentiation of gram-positive and gram-negative bacteria. J Clin Microbiol (2002) 1.14

Correlation of approximate entropy, bispectral index, and spectral edge frequency 95 (SEF95) with clinical signs of "anesthetic depth" during coadministration of propofol and remifentanil. Anesthesiology (2003) 1.13

Early up-regulation of chemokine expression in fulminant hepatic failure. J Pathol (2003) 1.11

Long-term follow-up of MRC Myeloma IX trial: Survival outcomes with bisphosphonate and thalidomide treatment. Clin Cancer Res (2013) 1.11

High-dose therapy and autologous stem cell transplantation in first relapse for diffuse large B cell lymphoma in the rituximab era: an analysis based on data from the European Blood and Marrow Transplantation Registry. Biol Blood Marrow Transplant (2011) 1.09

A practical update on the use of bortezomib in the management of multiple myeloma. Oncologist (2006) 1.09

Spectral entropy and bispectral index as measures of the electroencephalographic effects of sevoflurane. Anesthesiology (2004) 1.06

Overall survival and fungal infection-related mortality in patients with invasive fungal infection and neutropenia after myelosuppressive chemotherapy in a tertiary care centre from 1995 to 2006. J Antimicrob Chemother (2010) 1.05

Respiratory depression with tramadol in a patient with renal impairment and CYP2D6 gene duplication. Anesth Analg (2008) 1.05

Clinical validation of a new thermodilution system for the assessment of cardiac output and volumetric parameters. Crit Care (2012) 1.05

Favorable outcomes with alemtuzumab-conditioned unrelated donor stem cell transplantation in adults with high-risk Philadelphia chromosome-negative acute lymphoblastic leukemia in first complete remission. Haematologica (2009) 1.04

Cholecystitis in neutropenic patients: retrospective study and systematic review. Leuk Res (2006) 1.04

Autologous stem cell transplantation in elderly patients (> or =60 years) with diffuse large B-cell lymphoma: an analysis based on data in the European Blood and Marrow Transplantation registry. Haematologica (2008) 1.04

Assessment of cardiac output, intravascular volume status, and extravascular lung water by transpulmonary indicator dilution in critically ill neonates and infants. J Cardiothorac Vasc Anesth (2002) 1.02

Lenalidomide, adriamycin, and dexamethasone (RAD) in patients with relapsed and refractory multiple myeloma: a report from the German Myeloma Study Group DSMM (Deutsche Studiengruppe Multiples Myelom). Blood (2009) 1.02

Genetic factors in pain and its treatment. Curr Opin Anaesthesiol (2007) 1.01

A systematic review of phase II trials of thalidomide/dexamethasone combination therapy in patients with relapsed or refractory multiple myeloma. Eur J Haematol (2008) 1.00

CpG oligonucleotide activates Toll-like receptor 9 and causes lung inflammation in vivo. Respir Res (2007) 1.00

Priming with synthetic oligonucleotides attenuates pressure overload-induced inflammation and cardiac hypertrophy in mice. Cardiovasc Res (2012) 0.99

Screening of copy number polymorphisms in human beta-defensin genes using modified real-time quantitative PCR. J Immunol Methods (2005) 0.98

Making sense of itraconazole pharmacokinetics. J Antimicrob Chemother (2005) 0.98

Prone positioning, systemic hemodynamics, hepatic indocyanine green kinetics, and gastric intramucosal energy balance in patients with acute lung injury. Intensive Care Med (2001) 0.98

Early relapses in primary CNS lymphoma after response to polychemotherapy without intraventricular treatment: results of a phase II study. J Neurooncol (2008) 0.97

A phase 2 study of SRT501 (resveratrol) with bortezomib for patients with relapsed and or refractory multiple myeloma. Br J Haematol (2012) 0.97

Efficacy of caspofungin against invasive Candida or invasive Aspergillus infections in neutropenic patients. Cancer (2006) 0.96

Anesthesia or sedation for gastroenterologic endoscopies. Curr Opin Anaesthesiol (2009) 0.96

Toll-like receptor 4 modulates myocardial ischaemia-reperfusion injury: Role of matrix metalloproteinases. Eur J Heart Fail (2006) 0.96

Interrelationship of age and diet in Romania's oldest human burial. Naturwissenschaften (2012) 0.95

Association of genetic variants of methionine metabolism with methotrexate-induced CNS white matter changes in patients with primary CNS lymphoma. Neuro Oncol (2008) 0.95

Early complete response during chemotherapy predicts favorable outcome in patients with primary CNS lymphoma. Neuro Oncol (2010) 0.95

Tumour cell generation of inducible regulatory T-cells in multiple myeloma is contact-dependent and antigen-presenting cell-independent. PLoS One (2012) 0.95

Reduced immune effector cell NKG2D expression and increased levels of soluble NKG2D ligands in multiple myeloma may not be causally linked. Cancer Immunol Immunother (2009) 0.94