Published in Microb Cell Fact on June 14, 2010
Comparative characterization of two marine alginate lyases from Zobellia galactanivorans reveals distinct modes of action and exquisite adaptation to their natural substrate. J Biol Chem (2013) 1.14
Biochemical and structural characterization of the complex agarolytic enzyme system from the marine bacterium Zobellia galactanivorans. J Biol Chem (2012) 0.97
High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli. Microb Cell Fact (2013) 0.86
Mannitol-1-phosphate dehydrogenase activity in Ectocarpus siliculosus, a key role for mannitol synthesis in brown algae. Planta (2010) 0.84
High throughput quantitative expression screening and purification applied to recombinant disulfide-rich venom proteins produced in E. coli. J Vis Exp (2014) 0.83
Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms. Microb Cell Fact (2010) 0.82
The Vanadium Iodoperoxidase from the marine flavobacteriaceae species Zobellia galactanivorans reveals novel molecular and evolutionary features of halide specificity in the vanadium haloperoxidase enzyme family. Appl Environ Microbiol (2014) 0.82
The β-glucanase ZgLamA from Zobellia galactanivorans evolved a bent active site adapted for efficient degradation of algal laminarin. J Biol Chem (2013) 0.82
An extended network of genomic maintenance in the archaeon Pyrococcus abyssi highlights unexpected associations between eucaryotic homologs. PLoS One (2013) 0.80
Controlling carrageenan structure using a novel formylglycine-dependent sulfatase, an endo-4S-iota-carrageenan sulfatase. Mar Biotechnol (NY) (2012) 0.78
The mannitol utilization system of the marine bacterium Zobellia galactanivorans. Appl Environ Microbiol (2014) 0.78
A functional study of transforming growth factor-beta from the gonad of Pacific oyster Crassostrea gigas. Mar Biotechnol (NY) (2011) 0.78
Enzyme-Assisted Preparation of Furcellaran-Like κ-/β-Carrageenan. Mar Biotechnol (NY) (2015) 0.76
Discovery of a novel iota carrageenan sulfatase isolated from the marine bacterium Pseudoalteromonas carrageenovora. Front Chem (2014) 0.75
Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol (2001) 66.87
Improved prediction of signal peptides: SignalP 3.0. J Mol Biol (2004) 48.40
Protein production by auto-induction in high density shaking cultures. Protein Expr Purif (2005) 29.74
InterProScan: protein domains identifier. Nucleic Acids Res (2005) 18.82
The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins. Science (2002) 11.11
Recombinant protein expression in Escherichia coli. Curr Opin Biotechnol (1999) 4.59
Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features. Proc Natl Acad Sci U S A (2006) 4.52
Predicting protein function from sequence and structural data. Curr Opin Struct Biol (2005) 4.31
Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol (2002) 4.22
Complete genome sequence of the marine planctomycete Pirellula sp. strain 1. Proc Natl Acad Sci U S A (2003) 4.16
Protein production and purification. Nat Methods (2008) 3.97
Hydrophobic cluster analysis: procedures to derive structural and functional information from 2-D-representation of protein sequences. Biochimie (1990) 2.75
An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi. Mol Microbiol (2003) 2.75
The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins. Protein Expr Purif (2007) 2.10
Design of high-throughput methods of protein production for structural biology. Structure (2000) 2.09
Molecular toolbox for studying diatom biology in Phaeodactylum tricornutum. Gene (2007) 2.05
From structure to function: approaches and limitations. Nat Struct Biol (2000) 1.96
High-throughput proteomics: protein expression and purification in the postgenomic world. Protein Expr Purif (2001) 1.80
Recombinant protein expression and solubility screening in Escherichia coli: a comparative study. Acta Crystallogr D Biol Crystallogr (2006) 1.65
High throughput protein production for functional proteomics. Trends Biotechnol (2003) 1.45
Screening for soluble expression of recombinant proteins in a 96-well format. Anal Biochem (2001) 1.36
Structural genomics: from genes to structures with valuable materials and many questions in between. Nat Methods (2008) 1.33
Automated expression and solubility screening of His-tagged proteins in 96-well format. Anal Biochem (2005) 1.32
Purification of recombinant proteins with metal chelate adsorbent. Genet Eng (N Y) (1990) 1.19
Generating fusions to glutathione S-transferase for protein studies. Methods Enzymol (2000) 1.16
Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones. Microb Cell Fact (2005) 1.15
Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis. BMC Biotechnol (2008) 1.12
Comparison of small- and large-scale expression of selected Pyrococcus furiosus genes as an aid to high-throughput protein production. J Struct Funct Genomics (2005) 1.10
Analysis of the first genome of a hyperthermophilic marine virus-like particle, PAV1, isolated from Pyrococcus abyssi. J Bacteriol (2007) 0.97
An automated small-scale protein expression and purification screening provides beneficial information for protein production. J Struct Funct Genomics (2004) 0.94
A preliminary solubility screen used to improve crystallization trials: crystallization and preliminary X-ray structure determination of Aeropyrum pernix flap endonuclease-1. Acta Crystallogr D Biol Crystallogr (2004) 0.93
Structure and function of a novel endonuclease acting on branched DNA substrates. EMBO J (2009) 0.89
Expression of soluble recombinant proteins in a cell-free system using a 96-well format. J Biochem Biophys Methods (2003) 0.86
Structural genomics of eukaryotic targets at a laboratory scale. J Struct Funct Genomics (2005) 0.81
Protein production and crystallization at SECSG -- an overview. J Struct Funct Genomics (2005) 0.80
Pilot studies on the parallel production of soluble mouse proteins in a bacterial expression system. J Struct Funct Genomics (2005) 0.77
The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins. Science (2002) 11.11
The genome of the sea urchin Strongylocentrotus purpuratus. Science (2006) 6.41
Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota. Nature (2010) 5.08
Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features. Proc Natl Acad Sci U S A (2006) 4.52
The Ectocarpus genome and the independent evolution of multicellularity in brown algae. Nature (2010) 3.60
The tiny eukaryote Ostreococcus provides genomic insights into the paradox of plankton speciation. Proc Natl Acad Sci U S A (2007) 3.58
An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi. Mol Microbiol (2003) 2.75
Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls. BMC Plant Biol (2008) 2.35
Degradation of cellulose substrates by cellulosome chimeras. Substrate targeting versus proximity of enzyme components. J Biol Chem (2002) 2.27
Evolution and diversity of plant cell walls: from algae to flowering plants. Annu Rev Plant Biol (2011) 2.06
Environmental and gut bacteroidetes: the food connection. Front Microbiol (2011) 2.01
The diversity of small eukaryotic phytoplankton (< or =3 microm) in marine ecosystems. FEMS Microbiol Rev (2008) 1.83
Ecotype diversity in the marine picoeukaryote Ostreococcus (Chlorophyta, Prasinophyceae). Environ Microbiol (2005) 1.70
The three-dimensional structure of invertase (beta-fructosidase) from Thermotoga maritima reveals a bimodular arrangement and an evolutionary relationship between retaining and inverting glycosidases. J Biol Chem (2004) 1.64
Development and physiology of the brown alga Ectocarpus siliculosus: two centuries of research. New Phytol (2008) 1.63
Bioconversion of red seaweed galactans: a focus on bacterial agarases and carrageenases. Appl Microbiol Biotechnol (2006) 1.59
Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: biological implications for cell wall metabolism. Plant Cell (2007) 1.52
Cryptic sex in the smallest eukaryotic marine green alga. Mol Biol Evol (2010) 1.47
The conformation and function of a multimodular glycogen-degrading pneumococcal virulence factor. Structure (2011) 1.45
Novel fatty acid elongases and their use for the reconstitution of docosahexaenoic acid biosynthesis. J Lipid Res (2004) 1.39
The three-dimensional structures of two beta-agarases. J Biol Chem (2003) 1.38
Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress. Genome Biol (2009) 1.36
Genome structure and metabolic features in the red seaweed Chondrus crispus shed light on evolution of the Archaeplastida. Proc Natl Acad Sci U S A (2013) 1.36
Family 6 carbohydrate binding modules in beta-agarases display exquisite selectivity for the non-reducing termini of agarose chains. J Biol Chem (2006) 1.35
An original adaptation of photosynthesis in the marine green alga Ostreococcus. Proc Natl Acad Sci U S A (2008) 1.35
The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours. Biochem J (2005) 1.34
Structural basis of cellulosome efficiency explored by small angle X-ray scattering. J Biol Chem (2005) 1.32
Characterization of the first alginolytic operons in a marine bacterium: from their emergence in marine Flavobacteriia to their independent transfers to marine Proteobacteria and human gut Bacteroides. Environ Microbiol (2012) 1.30
The hyperthermophilic euryarchaeota Pyrococcus abyssi likely requires the two DNA polymerases D and B for DNA replication. J Mol Biol (2005) 1.29
Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas. Gene (2003) 1.27
Exploring nucleo-cytoplasmic large DNA viruses in Tara Oceans microbial metagenomes. ISME J (2013) 1.26
Life-cycle and genome of OtV5, a large DNA virus of the pelagic marine unicellular green alga Ostreococcus tauri. PLoS One (2008) 1.25
The family 6 carbohydrate binding module CmCBM6-2 contains two ligand-binding sites with distinct specificities. J Biol Chem (2004) 1.23
Protein disorder: conformational distribution of the flexible linker in a chimeric double cellulase. Biophys J (2005) 1.22
Parallel substrate binding sites in a beta-agarase suggest a novel mode of action on double-helical agarose. Structure (2004) 1.19
Genome-wide analysis of core cell cycle genes in the unicellular green alga Ostreococcus tauri. Mol Biol Evol (2004) 1.18
Starch division and partitioning. A mechanism for granule propagation and maintenance in the picophytoplanktonic green alga Ostreococcus tauri. Plant Physiol (2004) 1.17
Dimension, shape, and conformational flexibility of a two domain fungal cellulase in solution probed by small angle X-ray scattering. J Biol Chem (2002) 1.17
Structure of a full length psychrophilic cellulase from Pseudoalteromonas haloplanktis revealed by X-ray diffraction and small angle X-ray scattering. J Mol Biol (2005) 1.17
In vivo characterization of the first acyl-CoA Delta6-desaturase from a member of the plant kingdom, the microalga Ostreococcus tauri. Biochem J (2005) 1.15
New insights into the nature and phylogeny of prasinophyte antenna proteins: Ostreococcus tauri, a case study. Mol Biol Evol (2005) 1.15
The complete chloroplast and mitochondrial DNA sequence of Ostreococcus tauri: organelle genomes of the smallest eukaryote are examples of compaction. Mol Biol Evol (2007) 1.14
Comparative characterization of two marine alginate lyases from Zobellia galactanivorans reveals distinct modes of action and exquisite adaptation to their natural substrate. J Biol Chem (2013) 1.14
A genomewide survey of developmentally relevant genes in Ciona intestinalis. II. Genes for homeobox transcription factors. Dev Genes Evol (2003) 1.11
Gene functionalities and genome structure in Bathycoccus prasinos reflect cellular specializations at the base of the green lineage. Genome Biol (2012) 1.10
Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis. BMC Evol Biol (2008) 1.09
The crystal structure of the family 6 carbohydrate binding module from Cellvibrio mixtus endoglucanase 5a in complex with oligosaccharides reveals two distinct binding sites with different ligand specificities. J Biol Chem (2004) 1.08
A scissor blade-like closing mechanism implicated in transmembrane signaling in a Bacteroides hybrid two-component system. Proc Natl Acad Sci U S A (2012) 1.07
Crystal structure of inactivated Thermotoga maritima invertase in complex with the trisaccharide substrate raffinose. Biochem J (2006) 1.06
Novel insights into evolution of protistan polyketide synthases through phylogenomic analysis. Protist (2007) 1.06
Early gene duplication within chloroplastida and its correspondence with relocation of starch metabolism to chloroplasts. Genetics (2008) 1.06
Structural insights into the mechanism of formation of cellulosomes probed by small angle X-ray scattering. J Biol Chem (2004) 1.05
A novel proteomic approach identifies new interaction partners for proliferating cell nuclear antigen. J Mol Biol (2007) 1.04
A novel unsaturated β-glucuronyl hydrolase involved in ulvan degradation unveils the versatility of stereochemistry requirements in family GH105. J Biol Chem (2014) 1.03
TPV1, the first virus isolated from the hyperthermophilic genus Thermococcus. Environ Microbiol (2011) 1.02
Clues about the genetic basis of adaptation emerge from comparing the proteomes of two Ostreococcus ecotypes (Chlorophyta, Prasinophyceae). Mol Biol Evol (2008) 1.01
DNA polymerase switching on homotrimeric PCNA at the replication fork of the euryarchaea Pyrococcus abyssi. J Mol Biol (2007) 1.01
Carrageenan-induced innate immune response is modified by enzymes that hydrolyze distinct galactosidic bonds. J Nutr Biochem (2009) 1.01
Microwave assisted "click" chemistry for the synthesis of multiple labeled-carbohydrate oligonucleotides on solid support. J Org Chem (2006) 1.00
NADPH oxidases in Eukaryotes: red algae provide new hints! Curr Genet (2005) 1.00
Characterization of mannuronan C-5-epimerase genes from the brown alga Laminaria digitata. Plant Physiol (2003) 0.99
The translational repressor 4E-BP called to order by eIF4E: new structural insights by SAXS. Nucleic Acids Res (2010) 0.98
Discovery and structural characterization of a novel glycosidase family of marine origin. Environ Microbiol (2011) 0.98
Metagenomes of the picoalga Bathycoccus from the Chile coastal upwelling. PLoS One (2012) 0.98
Structure/function analysis of a type iii polyketide synthase in the brown alga Ectocarpus siliculosus reveals a biochemical pathway in phlorotannin monomer biosynthesis. Plant Cell (2013) 0.98
The heterodimeric primase from the euryarchaeon Pyrococcus abyssi: a multifunctional enzyme for initiation and repair? J Mol Biol (2007) 0.97
Biochemical and structural characterization of the complex agarolytic enzyme system from the marine bacterium Zobellia galactanivorans. J Biol Chem (2012) 0.97
Molecular diversity of new Thermococcales isolates from a single area of hydrothermal deep-sea vents as revealed by randomly amplified polymorphic DNA fingerprinting and 16S rRNA gene sequence analysis. Appl Environ Microbiol (2004) 0.97
Cloning and biochemical characterization of the fucanase FcnA: definition of a novel glycoside hydrolase family specific for sulfated fucans. Glycobiology (2006) 0.97
Structural insights into a new homodimeric self-activated GTPase family. EMBO Rep (2007) 0.97
Portrait of an enzyme, a complete structural analysis of a multimodular {beta}-N-acetylglucosaminidase from Clostridium perfringens. J Biol Chem (2009) 0.96
Response of the transcriptome of the intertidal red seaweed Chondrus crispus to controlled and natural stresses. New Phytol (2007) 0.96
Unusual number and genomic organization of Hox genes in the tunicate Ciona intestinalis. Gene (2003) 0.96
Mutational and structural analysis of aglycone specificity in maize and sorghum beta-glucosidases. J Biol Chem (2003) 0.95
D-aspartic acid in the nervous system of Aplysia limacina: possible role in neurotransmission. J Cell Physiol (2006) 0.95
Acquisition and maintenance of resistance to viruses in eukaryotic phytoplankton populations. Environ Microbiol (2011) 0.95
Diurnal oscillations of metabolite abundances and gene analysis provide new insights into central metabolic processes of the brown alga Ectocarpus siliculosus. New Phytol (2010) 0.95
Integrative analysis of metabolite and transcript abundance during the short-term response to saline and oxidative stress in the brown alga Ectocarpus siliculosus. Plant Cell Environ (2011) 0.94