Published in Proc Natl Acad Sci U S A on January 01, 1990
Effect of crown ethers on structure, stability, activity, and enantioselectivity of subtilisin Carlsberg in organic solvents. Biotechnol Bioeng (2001) 0.81
Interfacial activation-based molecular bioimprinting of lipolytic enzymes. Proc Natl Acad Sci U S A (1995) 0.77
Dual bioimprinting of Thermomyces lanuginosus lipase for synthesis of biodiesel. Biotechnol Rep (Amst) (2016) 0.75
Bioimprinting as a tool for the detection of aflatoxin B1 using a capacitive biosensor. Biotechnol Rep (Amst) (2016) 0.75
Hydration of proteins and polypeptides. Adv Protein Chem (1974) 6.25
Dynamics of proteins: elements and function. Annu Rev Biochem (1983) 4.56
Enzymatic catalysis in nonaqueous solvents. J Biol Chem (1988) 2.23
Enzymatic catalysis in organic media at 100 degrees C. Science (1984) 2.00
Enzymatic catalysis in anhydrous organic solvents. Trends Biochem Sci (1989) 1.71
The design of molecular hosts, guests, and their complexes. Science (1988) 1.36
A stereospecific cyclization catalyzed by an antibody. Science (1987) 1.31
Covalent coupling methods for inorganic support materials. Methods Enzymol (1976) 1.28
Model studies in molecular recognition. Science (1987) 1.10
Artificial enzymes. Science (1982) 1.08
Inhibitor-induced enzyme activation in organic solvents. J Biol Chem (1988) 1.01
The interplay between chemistry and biology in the design of enzymatic catalysts. Science (1988) 0.89
Designing surfaces that kill bacteria on contact. Proc Natl Acad Sci U S A (2001) 2.93
Visual evidence of acidic environment within degrading poly(lactic-co-glycolic acid) (PLGA) microspheres. Pharm Res (2000) 2.64
The mechanisms of irreversible enzyme inactivation at 100C. Science (1985) 2.28
Enzymatic catalysis in nonaqueous solvents. J Biol Chem (1988) 2.23
Kinetics of insulin aggregation in aqueous solutions upon agitation in the presence of hydrophobic surfaces. Proc Natl Acad Sci U S A (1991) 2.19
Enzyme-catalyzed processes in organic solvents. Proc Natl Acad Sci U S A (1985) 2.10
Enzymatic catalysis in organic media at 100 degrees C. Science (1984) 2.00
Non-viral gene therapy: polycation-mediated DNA delivery. Appl Microbiol Biotechnol (2003) 1.77
Enzymatic catalysis in anhydrous organic solvents. Trends Biochem Sci (1989) 1.71
Stabilization of enzymes against thermal inactivation. Adv Appl Microbiol (1983) 1.70
Why does ribonuclease irreversibly inactivate at high temperatures? Biochemistry (1986) 1.65
The effect of water on enzyme action in organic media. J Biol Chem (1988) 1.62
Cloning of an organic solvent-resistance gene in Escherichia coli: the unexpected role of alkylhydroperoxide reductase. Proc Natl Acad Sci U S A (1995) 1.57
Quantitative comparison of polyethylenimine formulations and adenoviral vectors in terms of intracellular gene delivery processes. Gene Ther (2005) 1.56
Enzyme crystal structure in a neat organic solvent. Proc Natl Acad Sci U S A (1993) 1.54
Lyophilization-induced reversible changes in the secondary structure of proteins. Proc Natl Acad Sci U S A (1995) 1.43
Protein stability in controlled-release systems. Nat Biotechnol (2000) 1.41
Peroxidase-catalyzed removal of phenols from coal-conversion waste waters. Science (1983) 1.40
Why is one Bacillus alpha-amylase more resistant against irreversible thermoinactivation than another? J Biol Chem (1988) 1.33
Immobilized enzymes and cells as practical catalysts. Science (1983) 1.27
Peroxidases depolymerize lignin in organic media but not in water. Proc Natl Acad Sci U S A (1986) 1.27
Enzyme stabilization by immobilization. Anal Biochem (1979) 1.26
Mechanisms of irreversible thermal inactivation of Bacillus alpha-amylases. J Biol Chem (1988) 1.21
The mechanochemistry of immobilized enzymes. How to steer a chemical process at the molecular level by a mechanical device. Biochim Biophys Acta (1974) 1.19
X-ray crystal structure of cross-linked subtilisin Carlsberg in water vs. acetonitrile. Biochem Biophys Res Commun (1994) 1.16
Dephenolization of industrial wastewaters catalyzed by polyphenol oxidase. Biotechnol Bioeng (1984) 1.13
Correct protein folding in glycerol. Proc Natl Acad Sci U S A (1997) 1.11
Solid-phase aggregation of proteins under pharmaceutically relevant conditions. J Pharm Sci (1994) 1.09
Moisture-induced aggregation of lyophilized proteins in the solid state. Biotechnol Bioeng (1991) 1.05
Thermal destruction processes in proteins involving cystine residues. J Biol Chem (1987) 1.05
On the pH memory of lyophilized compounds containing protein functional groups. Biotechnol Bioeng (1997) 1.04
Aggregation of a lyophilized pharmaceutical protein, recombinant human albumin: effect of moisture and stabilization by excipients. Biotechnology (N Y) (1995) 1.03
The principles of enzyme stabilization. I. Increase in thermostability of enzymes covalently bound to a complementary surface of a polymer support in a multipoint fashion. Biochim Biophys Acta (1977) 1.02
Enzyme thermoinactivation in anhydrous organic solvents. Biotechnol Bioeng (1991) 1.01
Inhibitor-induced enzyme activation in organic solvents. J Biol Chem (1988) 1.01
Can conformational changes be responsible for solvent and excipient effects on the catalytic behavior of subtilisin Carlsberg in organic solvents? Biotechnol Bioeng (1997) 1.00
Mechanism of insulin aggregation and stabilization in agitated aqueous solutions. Biotechnol Bioeng (1992) 1.00
The solvent dependence of enzyme specificity. Biochim Biophys Acta (1994) 0.99
Structure, thermostability, and conformational flexibility of hen egg-white lysozyme dissolved in glycerol. Proc Natl Acad Sci U S A (1999) 0.98
FTIR characterization of the secondary structure of proteins encapsulated within PLGA microspheres. J Control Release (1999) 0.98
On protein solubility in organic solvent. Biotechnol Bioeng (1994) 0.96
Enzyme memory. What is remembered and why? Nature (1995) 0.96
Correlation between catalytic activity and secondary structure of subtilisin dissolved in organic solvents. Biotechnol Bioeng (1997) 0.95
Solid-state NMR assessment of enzyme active center structure under nonaqueous conditions. J Biol Chem (1992) 0.95
Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water. Proc Natl Acad Sci U S A (1998) 0.95
Detergent-enabled transport of proteins and nucleic acids through hydrophobic solvents. Proc Natl Acad Sci U S A (1994) 0.94
Control of oligomeric enzyme thermostability by protein engineering. Proc Natl Acad Sci U S A (1987) 0.94
Stabilization of tetanus and diphtheria toxoids against moisture-induced aggregation. Proc Natl Acad Sci U S A (1995) 0.94
Molecular-modeling calculations of enzymatic enantioselectivity taking hydration into account. Biotechnol Bioeng (1998) 0.92
Calorimetric evidence for a native-like conformation of hen egg-white lysozyme dissolved in glycerol. Biochim Biophys Acta (2000) 0.92
Moisture-induced aggregation of lyophilized insulin. Pharm Res (1994) 0.92
Structure of lysozyme dissolved in neat organic solvents as assessed by NMR and CD spectroscopies. Biotechnol Bioeng (1999) 0.92
Fourier-transform infrared spectroscopic investigation of protein stability in the lyophilized form. Biochim Biophys Acta (1995) 0.91
Solid-state nuclear magnetic resonance investigation of solvent dependence of tyrosyl ring motion in an enzyme. Biotechnol Bioeng (1993) 0.91
Polymerization of phenols catalyzed by peroxidase in nonaqueous media. Biotechnol Bioeng (1987) 0.91
Structural stability of DNA in nonaqueous solvents. Biotechnol Bioeng (2000) 0.90
Transport of proteins dissolved in organic solvents across biomimetic membranes. Proc Natl Acad Sci U S A (1995) 0.89
Antibody-antigen binding in organic solvents. Biochem Biophys Res Commun (1989) 0.89
Protein separation and purification in neat dimethyl sulfoxide. Biochem Biophys Res Commun (1991) 0.88
Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectants. Biotechnol Bioeng (1993) 0.88
Stabilization of poliovirus against heat inactivation. Biochem Biophys Res Commun (1989) 0.88
The crystal structure of subtilisin Carlsberg in anhydrous dioxane and its comparison with those in water and acetonitrile. Proc Natl Acad Sci U S A (1997) 0.87
On enzymatic activity in organic solvents as a function of enzyme history. Biotechnol Bioeng (1998) 0.87
Improving biomaterial properties of collagen films by chemical modification. Biotechnol Bioeng (2001) 0.86
Subunit interface of triosephosphate isomerase: site-directed mutagenesis and characterization of the altered enzyme. Biochemistry (1987) 0.86
Rapid detection of E. coli immobilized in gel microdroplets. Ann N Y Acad Sci (1987) 0.85
Striking activation of oxidative enzymes suspended in nonaqueous media. Proc Natl Acad Sci U S A (1999) 0.85
Production of biosurfactants from sugar alcohols and vegetable oils catalyzed by lipases in a nonaqueous medium. Biotechnol Bioeng (1988) 0.84
The principles of enzyme stabilization. IV. Modification of 'key' functional groups in the tertiary structure of proteins. Biochim Biophys Acta (1979) 0.84
The effect of electron carriers and other ligands on oxygen stability of clostridial hydrogenase. Biochim Biophys Acta (1981) 0.84
Deterioration of lyophilized pharmaceutical proteins. Biochemistry (Mosc) (1998) 0.84
The principles of enzyme stabilization. II. Increase in the thermostability of enzymes as a result of multipoint noncovalent interaction with a polymeric support. Biochim Biophys Acta (1977) 0.84
Enzymatic oxidation of ethanol in the gaseous phase. Biotechnol Bioeng (1989) 0.83
Remarkable activation of enzymes in nonaqueous media by denaturing organic cosolvents. Biotechnol Bioeng (1996) 0.83
Inactivation and stabilization of stabilisins in neat organic solvents. Biotechnol Bioeng (1991) 0.83
Enzymatic analyses in organic solvents. Biotechnol Bioeng (1986) 0.82
Chelating agents protect hydrogenase against oxygen inactivation. Biochim Biophys Acta (1979) 0.82
Comparison of different strategies for the lipase-catalyzed preparative resolution of racemic acids and alcohols: Asymmetric hydrolysis, esterification, and transesterification. Biotechnol Bioeng (1984) 0.81
Enzymatic removal of bilirubin from blood: a potential treatment for neonatal jaundice. Science (1985) 0.81
Enzymes in organic solvents. Biochem Soc Trans (1989) 0.81
Analysis of processes causing thermal inactivation of enzymes. Methods Biochem Anal (1988) 0.81
Are water-immiscibility and apolarity of the solvent relevant to enzyme efficiency? Biotechnol Bioeng (1993) 0.81
A rationale for stabilization of oxygen-labile enzymes: application to a clostridial hydrogenase. Proc Natl Acad Sci U S A (1978) 0.81
Stereospecific oxidation of aliphatic alcohols catalyzed by galactose oxidase. Biochem Biophys Res Commun (1982) 0.81
Organic solvent binding to crystalline subtilisin1 in mostly aqueous media and in the neat solvents. Biochem Biophys Res Commun (1998) 0.81
Enzymatic mechanochemistry: a new approach to studying the mechanism of enzyme action. Biochim Biophys Acta (1976) 0.81
Enzyme-catalyzed, gas-phase reactions. Appl Biochem Biotechnol (1987) 0.80
On the role of reversible denaturation (unfolding) in the irreversible thermal inactivation of enzymes. Biotechnol Bioeng (1983) 0.79
On the mechanism of irreversible thermoinactivation of enzymes and possibilities for reactivation of "irreversibly" inactivated enzymes. Biochem Biophys Res Commun (1978) 0.79
Inhalation delivery of proteins from ethanol suspensions. Proc Natl Acad Sci U S A (2001) 0.79
Structural basis for the molecular memory of imprinted proteins in anhydrous media. Biotechnol Bioeng (1996) 0.78
Reusable evanescent wave DNA biosensor for rapid, highly sensitive, and selective detection of mercury ions. Biosens Bioelectron (2011) 0.78
Computer-assisted modeling of subtilisin enantioselectivity in organic solvents. Biotechnol Bioeng (1992) 0.78
The secondary structure and aggregation of lyophilized tetanus toxoid. J Pharm Sci (1996) 0.78
Thermodynamic analysis of solvent effect on substrate specificity of lyophilized enzymes suspended in organic media. Biotechnol Bioeng (1997) 0.78
Protein refolding in predominantly organic media markedly enhanced by common salts. Biotechnol Bioeng (1999) 0.78
Peroxidase-catalyzed asymmetric sulfoxidation in organic solvents versus in water. Biotechnol Bioeng (2000) 0.78
Effect of support material and enzyme pretreatment on enantioselectivity of immobilized subtilisin in organic solvents. Biotechnol Bioeng (1994) 0.78
Improving lipase enantioselectivity in organic solvents by forming substrate salts with chiral agents. Biotechnol Bioeng (2000) 0.78
On the issue of interfacial activation of lipase in nonaqueous media. Biotechnol Bioeng (1996) 0.78