Published in J Biol Chem on March 05, 1987
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Visual evidence of acidic environment within degrading poly(lactic-co-glycolic acid) (PLGA) microspheres. Pharm Res (2000) 2.64
The mechanisms of irreversible enzyme inactivation at 100C. Science (1985) 2.28
Enzymatic catalysis in nonaqueous solvents. J Biol Chem (1988) 2.23
Kinetics of insulin aggregation in aqueous solutions upon agitation in the presence of hydrophobic surfaces. Proc Natl Acad Sci U S A (1991) 2.19
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The effect of water on enzyme action in organic media. J Biol Chem (1988) 1.62
Cloning of an organic solvent-resistance gene in Escherichia coli: the unexpected role of alkylhydroperoxide reductase. Proc Natl Acad Sci U S A (1995) 1.57
Quantitative comparison of polyethylenimine formulations and adenoviral vectors in terms of intracellular gene delivery processes. Gene Ther (2005) 1.56
Enzyme crystal structure in a neat organic solvent. Proc Natl Acad Sci U S A (1993) 1.54
Lyophilization-induced reversible changes in the secondary structure of proteins. Proc Natl Acad Sci U S A (1995) 1.43
Protein stability in controlled-release systems. Nat Biotechnol (2000) 1.41
Peroxidase-catalyzed removal of phenols from coal-conversion waste waters. Science (1983) 1.40
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Immobilized enzymes and cells as practical catalysts. Science (1983) 1.27
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Enzyme stabilization by immobilization. Anal Biochem (1979) 1.26
Nature of the interaction of heparin with acidic fibroblast growth factor. Biochemistry (1993) 1.25
Mechanisms of irreversible thermal inactivation of Bacillus alpha-amylases. J Biol Chem (1988) 1.21
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X-ray crystal structure of cross-linked subtilisin Carlsberg in water vs. acetonitrile. Biochem Biophys Res Commun (1994) 1.16
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Dephenolization of industrial wastewaters catalyzed by polyphenol oxidase. Biotechnol Bioeng (1984) 1.13
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Formulation design of acidic fibroblast growth factor. Pharm Res (1993) 1.07
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Moisture-induced aggregation of lyophilized proteins in the solid state. Biotechnol Bioeng (1991) 1.05
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Aggregation of a lyophilized pharmaceutical protein, recombinant human albumin: effect of moisture and stabilization by excipients. Biotechnology (N Y) (1995) 1.03
Complement subcomponent C1q secreted by cultured human monocytes has subunit structure identical with that of serum C1q. Biochem J (1986) 1.03
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Inhibitor-induced enzyme activation in organic solvents. J Biol Chem (1988) 1.01
Enzyme thermoinactivation in anhydrous organic solvents. Biotechnol Bioeng (1991) 1.01
Can conformational changes be responsible for solvent and excipient effects on the catalytic behavior of subtilisin Carlsberg in organic solvents? Biotechnol Bioeng (1997) 1.00
Mechanism of insulin aggregation and stabilization in agitated aqueous solutions. Biotechnol Bioeng (1992) 1.00
The solvent dependence of enzyme specificity. Biochim Biophys Acta (1994) 0.99
Enhancement of DNA vaccine potency using conventional aluminum adjuvants. Vaccine (1999) 0.98
Structure, thermostability, and conformational flexibility of hen egg-white lysozyme dissolved in glycerol. Proc Natl Acad Sci U S A (1999) 0.98
FTIR characterization of the secondary structure of proteins encapsulated within PLGA microspheres. J Control Release (1999) 0.98
On protein solubility in organic solvent. Biotechnol Bioeng (1994) 0.96
Enzyme memory. What is remembered and why? Nature (1995) 0.96
Correlation between catalytic activity and secondary structure of subtilisin dissolved in organic solvents. Biotechnol Bioeng (1997) 0.95
Solid-state NMR assessment of enzyme active center structure under nonaqueous conditions. J Biol Chem (1992) 0.95
Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water. Proc Natl Acad Sci U S A (1998) 0.95
Control of oligomeric enzyme thermostability by protein engineering. Proc Natl Acad Sci U S A (1987) 0.94
Detergent-enabled transport of proteins and nucleic acids through hydrophobic solvents. Proc Natl Acad Sci U S A (1994) 0.94
Stabilization of tetanus and diphtheria toxoids against moisture-induced aggregation. Proc Natl Acad Sci U S A (1995) 0.94
Bacteriophage T7 RNA polymerase and its active-site mutants. Kinetic, spectroscopic and calorimetric characterization. J Mol Biol (1994) 0.93
Molecular-modeling calculations of enzymatic enantioselectivity taking hydration into account. Biotechnol Bioeng (1998) 0.92
Moisture-induced aggregation of lyophilized insulin. Pharm Res (1994) 0.92
Calorimetric evidence for a native-like conformation of hen egg-white lysozyme dissolved in glycerol. Biochim Biophys Acta (2000) 0.92
Structure of lysozyme dissolved in neat organic solvents as assessed by NMR and CD spectroscopies. Biotechnol Bioeng (1999) 0.92
Fourier-transform infrared spectroscopic investigation of protein stability in the lyophilized form. Biochim Biophys Acta (1995) 0.91
Polymerization of phenols catalyzed by peroxidase in nonaqueous media. Biotechnol Bioeng (1987) 0.91
Solid-state nuclear magnetic resonance investigation of solvent dependence of tyrosyl ring motion in an enzyme. Biotechnol Bioeng (1993) 0.91
Structural stability of DNA in nonaqueous solvents. Biotechnol Bioeng (2000) 0.90
Transport of proteins dissolved in organic solvents across biomimetic membranes. Proc Natl Acad Sci U S A (1995) 0.89
Antibody-antigen binding in organic solvents. Biochem Biophys Res Commun (1989) 0.89
Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectants. Biotechnol Bioeng (1993) 0.88
Protein separation and purification in neat dimethyl sulfoxide. Biochem Biophys Res Commun (1991) 0.88
Stabilization of poliovirus against heat inactivation. Biochem Biophys Res Commun (1989) 0.88
Production of abiotic receptors by molecular imprinting of proteins. Proc Natl Acad Sci U S A (1990) 0.87
Effect of polyanions on the unfolding of acidic fibroblast growth factor. Biochemistry (1993) 0.87
The crystal structure of subtilisin Carlsberg in anhydrous dioxane and its comparison with those in water and acetonitrile. Proc Natl Acad Sci U S A (1997) 0.87
On enzymatic activity in organic solvents as a function of enzyme history. Biotechnol Bioeng (1998) 0.87
Improving biomaterial properties of collagen films by chemical modification. Biotechnol Bioeng (2001) 0.86
Subunit interface of triosephosphate isomerase: site-directed mutagenesis and characterization of the altered enzyme. Biochemistry (1987) 0.86
Rapid detection of E. coli immobilized in gel microdroplets. Ann N Y Acad Sci (1987) 0.85
Striking activation of oxidative enzymes suspended in nonaqueous media. Proc Natl Acad Sci U S A (1999) 0.85
Origin of the isoelectric heterogeneity of monoclonal immunoglobulin h1B4. Pharm Res (1993) 0.84
Production of biosurfactants from sugar alcohols and vegetable oils catalyzed by lipases in a nonaqueous medium. Biotechnol Bioeng (1988) 0.84
The principles of enzyme stabilization. IV. Modification of 'key' functional groups in the tertiary structure of proteins. Biochim Biophys Acta (1979) 0.84
The effect of electron carriers and other ligands on oxygen stability of clostridial hydrogenase. Biochim Biophys Acta (1981) 0.84
Formulation, stability, and delivery of live attenuated vaccines for human use. Crit Rev Ther Drug Carrier Syst (1999) 0.84
Deterioration of lyophilized pharmaceutical proteins. Biochemistry (Mosc) (1998) 0.84
The principles of enzyme stabilization. II. Increase in the thermostability of enzymes as a result of multipoint noncovalent interaction with a polymeric support. Biochim Biophys Acta (1977) 0.84
Partially structured self-associating states of acidic fibroblast growth factor. Biochemistry (1993) 0.83
Inactivation and stabilization of stabilisins in neat organic solvents. Biotechnol Bioeng (1991) 0.83
Remarkable activation of enzymes in nonaqueous media by denaturing organic cosolvents. Biotechnol Bioeng (1996) 0.83
Enzymatic oxidation of ethanol in the gaseous phase. Biotechnol Bioeng (1989) 0.83
Enzymatic analyses in organic solvents. Biotechnol Bioeng (1986) 0.82
Stabilization of the FK506 binding protein by ligand binding. Biochem Biophys Res Commun (1991) 0.82
Chelating agents protect hydrogenase against oxygen inactivation. Biochim Biophys Acta (1979) 0.82
Ultraviolet absorption spectroscopy. Methods Mol Biol (1995) 0.82
Comparison of different strategies for the lipase-catalyzed preparative resolution of racemic acids and alcohols: Asymmetric hydrolysis, esterification, and transesterification. Biotechnol Bioeng (1984) 0.81
A rationale for stabilization of oxygen-labile enzymes: application to a clostridial hydrogenase. Proc Natl Acad Sci U S A (1978) 0.81
Enzymatic mechanochemistry: a new approach to studying the mechanism of enzyme action. Biochim Biophys Acta (1976) 0.81
Are water-immiscibility and apolarity of the solvent relevant to enzyme efficiency? Biotechnol Bioeng (1993) 0.81
Stereospecific oxidation of aliphatic alcohols catalyzed by galactose oxidase. Biochem Biophys Res Commun (1982) 0.81
Enzymes in organic solvents. Biochem Soc Trans (1989) 0.81
Analysis of processes causing thermal inactivation of enzymes. Methods Biochem Anal (1988) 0.81
Organic solvent binding to crystalline subtilisin1 in mostly aqueous media and in the neat solvents. Biochem Biophys Res Commun (1998) 0.81
Enzymatic removal of bilirubin from blood: a potential treatment for neonatal jaundice. Science (1985) 0.81
Enzyme-catalyzed, gas-phase reactions. Appl Biochem Biotechnol (1987) 0.80