Published in Appl Microbiol Biotechnol on June 23, 2011
The metabolic potential of Escherichia coli BL21 in defined and rich medium. Microb Cell Fact (2014) 0.97
Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli. Microb Cell Fact (2015) 0.95
Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors. J Biol Eng (2015) 0.84
A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity. Microb Cell Fact (2015) 0.84
Mechanisms of allosteric activation and inhibition of the deoxyribonucleoside triphosphate triphosphohydrolase from Enterococcus faecalis. J Biol Chem (2013) 0.83
Soluble expression, purification, and characterization of active recombinant human tissue plasminogen activator by auto-induction in E. coli. BMC Biotechnol (2015) 0.79
Smart sustainable bottle (SSB) system for E. coli based recombinant protein production. Microb Cell Fact (2014) 0.77
Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome. Microb Cell Fact (2017) 0.75
High-throughput strategies for penicillin G acylase production in rE. coli fed-batch cultivations. BMC Biotechnol (2014) 0.75
Screening proteins for NMR suitability. Methods Mol Biol (2014) 0.75
Gamma-Glutamylpolyamine Synthetase GlnA3 Is Involved in the First Step of Polyamine Degradation Pathway in Streptomyces coelicolor M145. Front Microbiol (2017) 0.75
Auto-inducing media for uniform isotope labeling of proteins with (15)N, (13)C and (2)H. J Biomol NMR (2015) 0.75
Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. Nat Biotechnol (2007) 6.21
Structure of the human receptor tyrosine kinase met in complex with the Listeria invasion protein InlB. Cell (2007) 2.02
Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. Microb Cell Fact (2008) 1.89
VEGFR-1-selective VEGF homologue PlGF is arteriogenic: evidence for a monocyte-mediated mechanism. Circ Res (2003) 1.82
Extending the host range of Listeria monocytogenes by rational protein design. Cell (2007) 1.76
Crystal structure of 5-aminolevulinate synthase, the first enzyme of heme biosynthesis, and its link to XLSA in humans. EMBO J (2005) 1.74
Stress induced by recombinant protein production in Escherichia coli. Adv Biochem Eng Biotechnol (2004) 1.69
Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins. Microb Cell Fact (2004) 1.64
Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. I. Growth-rate-dependent metabolic efficiency at steady state. Microbiology (2005) 1.42
Preparation of bioactive soluble human leukemia inhibitory factor from recombinant Escherichia coli using thioredoxin as fusion partner. Protein Expr Purif (2010) 1.22
Treatment of a case of primary retroperitoneal mucinous cystadenocarcinoma: is adjuvant hysterectomy and bilateral salpingo-oophorectomy justified? Am J Obstet Gynecol (2002) 1.14
The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate. Microb Cell Fact (2010) 1.10
Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. II. Dynamic response to famine and feast, activation of the methylglyoxal pathway and oscillatory behaviour. Microbiology (2005) 1.08
Comparative genomic analysis of dha regulon and related genes for anaerobic glycerol metabolism in bacteria. Biotechnol Prog (2003) 1.03
Renaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli. J Biotechnol (2002) 1.02
Structure of the type III secretion recognition protein YscU from Yersinia enterocolitica. J Mol Biol (2008) 1.01
Side effects of chaperone gene co-expression in recombinant protein production. Microb Cell Fact (2010) 1.01
Metabolic peculiarities of Aspergillus niger disclosed by comparative metabolic genomics. Genome Biol (2007) 0.99
Metabolic adaptation of Escherichia coli during temperature-induced recombinant protein production: 1. Readjustment of metabolic enzyme synthesis. Biotechnol Bioeng (2002) 0.99
The effect of an rhBMP-2 absorbable collagen sponge-targeted system on bone formation in vivo. Biomaterials (2009) 0.98
Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin. Microb Cell Fact (2010) 0.97
Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen. Microb Cell Fact (2011) 0.96
Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate. Proc Natl Acad Sci U S A (2014) 0.96
Opposing roles of Akt and STAT3 in the protection of the maternal heart from peripartum stress. Cardiovasc Res (2014) 0.95
Response of fluxome and metabolome to temperature-induced recombinant protein synthesis in Escherichia coli. J Biotechnol (2007) 0.95
Metabolic adaptation of Escherichia coli during temperature-induced recombinant protein production: 2. Redirection of metabolic fluxes. Biotechnol Bioeng (2002) 0.92
In-depth analysis of the Aspergillus niger glucoamylase (glaA) promoter performance using high-throughput screening and controlled bioreactor cultivation techniques. J Biotechnol (2008) 0.91
Metabolic costs of amino acid and protein production in Escherichia coli. Biotechnol J (2013) 0.91
Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes. Microb Cell Fact (2012) 0.91
Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen. Microb Cell Fact (2009) 0.91
Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes. Chembiochem (2012) 0.91
Patients' perception of preoperative information by interactive computer program-exemplified by cholecystectomy. Patient Educ Couns (2005) 0.90
Functional inclusion bodies produced in bacteria as naturally occurring nanopills for advanced cell therapies. Adv Mater (2012) 0.89
More than just a metabolic regulator--elucidation and validation of new targets of PdhR in Escherichia coli. BMC Syst Biol (2011) 0.88
Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development. PLoS One (2011) 0.88
Roles of heat-shock chaperones in the production of recombinant proteins in Escherichia coli. Adv Biochem Eng Biotechnol (2004) 0.87
Roimatacene: an antibiotic against Gram-negative bacteria isolated from Cystobacter ferrugineus Cb G35 (Myxobacteria). Chemistry (2011) 0.87
Packaging protein drugs as bacterial inclusion bodies for therapeutic applications. Microb Cell Fact (2012) 0.86
Raver1 is an integral component of muscle contractile elements. Cell Tissue Res (2006) 0.86
Arranged sevenfold: structural insights into the C-terminal oligomerization domain of human C4b-binding protein. J Mol Biol (2012) 0.85
Production of a polyester degrading extracellular hydrolase from Thermomonospora fusca. Biotechnol Prog (2002) 0.85
Purification of hepatitis B surface antigen virus-like particles from recombinant Pichia pastoris and in vivo analysis of their immunogenic properties. J Chromatogr B Analyt Technol Biomed Life Sci (2013) 0.85
Elansolid A3, a unique p-quinone methide antibiotic from Chitinophaga sancti. Chemistry (2011) 0.84
Glycoprotein production for structure analysis with stable, glycosylation mutant CHO cell lines established by fluorescence-activated cell sorting. Protein Sci (2010) 0.84
In situ multi-wavelength fluorescence spectroscopy as effective tool to simultaneously monitor spore germination, metabolic activity and quantitative protein production in recombinant Aspergillus niger fed-batch cultures. J Biotechnol (2007) 0.84
Optimized procedure to generate heavy isotope and selenomethionine-labeled proteins for structure determination using Escherichia coli-based expression systems. Appl Microbiol Biotechnol (2011) 0.83
Optimized procedure for renaturation of recombinant human bone morphogenetic protein-2 at high protein concentration. Biotechnol Bioeng (2004) 0.82
A novel functional assay for fungal histidine kinases group III reveals the role of HAMP domains for fungicide sensitivity. J Biotechnol (2011) 0.81
Improved production of medium-chain-length polyhydroxyalkanoates in glucose-based fed-batch cultivations of metabolically engineered Pseudomonas putida strains. J Microbiol Biotechnol (2014) 0.81
Supramolecular organization of protein-releasing functional amyloids solved in bacterial inclusion bodies. Acta Biomater (2012) 0.80
Application of an SPR-based receptor assay for the determination of biologically active recombinant bone morphogenetic protein-2. Anal Bioanal Chem (2005) 0.80
Folding and dimerization kinetics of bone morphogenetic protein-2, a member of the transforming growth factor-β family. FEBS J (2012) 0.80
Discovery and investigation of a new, second triose phosphate isomerase in Klebsiella pneumoniae. J Biotechnol (2006) 0.80
Multi-host expression system for recombinant production of challenging proteins. PLoS One (2013) 0.80
A nanostructured bacterial bioscaffold for the sustained bottom-up delivery of protein drugs. Nanomedicine (Lond) (2013) 0.79
Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca in Escherichia coli. Bioprocess Biosyst Eng (2006) 0.79
An engineered heparin-binding form of VEGF-E (hbVEGF-E). Biological effects in vitro and mobilizatiion of precursor cells. Angiogenesis (2003) 0.79
Comparison of a production process in a membrane-aerated stirred tank and up to 1000-L airlift bioreactors using BHK-21 cells and chemically defined protein-free medium. Biotechnol Prog (2003) 0.78
Structural analysis of human soluble adenylyl cyclase and crystal structures of its nucleotide complexes-implications for cyclase catalysis and evolution. FEBS J (2014) 0.78
Functional knock down of VCAM1 in mice mediated by endoplasmatic reticulum retained intrabodies. MAbs (2014) 0.77
Inclusion bodies of fuculose-1-phosphate aldolase as stable and reusable biocatalysts. Biotechnol Prog (2012) 0.76
Conformational and functional variants of CD44-targeted protein nanoparticles bio-produced in bacteria. Biofabrication (2016) 0.76
Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part I: selection of chromatographic system and estimation of adsorption isotherms. J Chromatogr A (2011) 0.76
Expression and purification of bioactive soluble murine stem cell factor from recombinant Escherichia coli using thioredoxin as fusion partner. J Biotechnol (2011) 0.76
High affinity peptide inhibitors of the hepatitis C virus NS3-4A protease refractory to common resistant mutants. J Biol Chem (2012) 0.75
Recombinant protein purification using gradient assisted simulated moving bed hydrophobic interaction chromatography. Part II: process design and experimental validation. J Chromatogr A (2011) 0.75
Biochemical characterization of molybdenum cofactor-free nitrate reductase from Neurospora crassa. J Biol Chem (2013) 0.75
Dengue-specific subviral nanoparticles: design, creation and characterization. J Nanobiotechnology (2013) 0.75
Kinetics control preferential heterodimer formation of platelet-derived growth factor from unfolded A- and B-chains. J Biol Chem (2003) 0.75
Hyafurones, hyapyrrolines, and hyapyrones: polyketides from Hyalangium minutum. J Nat Prod (2014) 0.75
Discontinuous and continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 from renaturation batches. J Chromatogr A (2006) 0.75
Sulfangolids, macrolide sulfate esters from Sorangium cellulosum. Chemistry (2012) 0.75
Change of extracellular cAMP concentration is a sensitive reporter for bacterial fitness in high-cell-density cultures of Escherichia coli. Biotechnol Bioeng (2004) 0.75
Spontaneous intraperitoneal hemorrhage. Surgery (2002) 0.75
Step gradients in 3-zone simulated moving bed chromatography. Application to the purification of antibodies and bone morphogenetic protein-2. J Chromatogr A (2007) 0.75