Intramolecular transposition by a synthetic IS50 (Tn5) derivative.

PubWeight™: 1.16‹?› | Rank: Top 10%

🔗 View Article (PMC 526819)

Published in J Bacteriol on November 01, 1990

Authors

T Tomcsanyi1, C M Berg, S H Phadnis, D E Berg

Author Affiliations

1: Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110-1093.

Articles citing this

Tn5/IS50 target recognition. Proc Natl Acad Sci U S A (1998) 2.47

Chromosome transfer in Rhodobacter sphaeroides: Hfr formation and genetic evidence for two unique circular chromosomes. J Bacteriol (1992) 1.60

Insertion of mini-IS605 and deletion of adjacent sequences in the nitroreductase (rdxA) gene cause metronidazole resistance in Helicobacter pylori NCTC11637. Antimicrob Agents Chemother (1999) 1.48

IS10/Tn10 transposition efficiently accommodates diverse transposon end configurations. EMBO J (1996) 1.29

Cluster of type IV secretion genes in Helicobacter pylori's plasticity zone. J Bacteriol (2003) 1.26

Implications of Tn5-associated adjacent deletions. J Bacteriol (1993) 1.08

MudSacI, a transposon with strong selectable and counterselectable markers: use for rapid mapping of chromosomal mutations in Salmonella typhimurium. J Bacteriol (1995) 1.06

Inversions and deletions generated by a mini-gamma delta (Tn1000) transposon. J Bacteriol (1994) 0.99

pDUAL: a transposon-based cosmid cloning vector for generating nested deletions and DNA sequencing templates in vivo. Proc Natl Acad Sci U S A (1993) 0.98

Simple and efficient generation in vitro of nested deletions and inversions: Tn5 intramolecular transposition. Nucleic Acids Res (1998) 0.93

A gene truncation strategy generating N- and C-terminal deletion variants of proteins for functional studies: mapping of the Sec1p binding domain in yeast Mso1p by a Mu in vitro transposition-based approach. Nucleic Acids Res (2005) 0.90

Suicidal autointegration of sleeping beauty and piggyBac transposons in eukaryotic cells. PLoS Genet (2014) 0.87

New nucleotide sequence data on the EMBL File Server. Nucleic Acids Res (1991) 0.75

MuA-mediated in vitro cloning of circular DNA: transpositional autointegration and the effect of MuB. Mol Genet Genomics (2016) 0.75

Articles cited by this

DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A (1977) 790.54

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene (1982) 102.30

Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol (1980) 41.18

Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proc Natl Acad Sci U S A (1979) 7.80

Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. J Bacteriol (1985) 6.72

An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in gram-negative bacteria by marker exchange-eviction mutagenesis. Gene (1987) 5.25

The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites. Mol Gen Genet (1985) 3.80

A correction in the nucleotide sequence of the Tn903 kanamycin resistance determinant in pUC4K. Nucleic Acids Res (1988) 3.47

Specificity of transposon Tn5 insertion. Genetics (1983) 3.04

Excision and insertion of the conjugative transposon Tn916 involves a novel recombination mechanism. Cell (1989) 2.91

Transcriptional and translational initiation sites of IS50. Control of transposase and inhibitor expression. J Mol Biol (1986) 2.64

DNA sequences at the ends of transposon Tn5 required for transposition. Nature (1983) 2.32

Effect of dam methylation on Tn5 transposition. J Mol Biol (1988) 2.02

Sequences essential for transposition at the termini of IS50. Proc Natl Acad Sci U S A (1983) 1.96

Structural requirement for IS50-mediated gene transposition. Proc Natl Acad Sci U S A (1983) 1.96

Identification of base pairs in the outside end of insertion sequence IS50 that are needed for IS50 and Tn5 transposition. Proc Natl Acad Sci U S A (1987) 1.70

Factors affecting transposition activity of IS50 and Tn5 ends. Gene (1989) 1.67

Oligonucleotide-directed double-strand break repair in plasmids of Escherichia coli: a method for site-specific mutagenesis. Proc Natl Acad Sci U S A (1986) 1.61

Transposon Tn5 target specificity: preference for insertion at G/C pairs. Genetics (1988) 1.41

recA-independent recombination between repeated IS50 elements is not caused by an IS50-encoded function. J Bacteriol (1985) 1.10

Use of transposon-promoted deletions in DNA sequence analysis. Methods Enzymol (1987) 1.04

Specificity of deletion events in pBR322. Plasmid (1989) 1.01

Transposition effect of adenine (Dam) methylation on activity of O end mutants of IS50. J Mol Biol (1989) 0.87

Tn5 transposes independently of cointegrate resolution. Evidence for an alternative model for transposition. J Mol Biol (1985) 0.86

Articles by these authors

The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature (1997) 34.00

Transposition of R factor genes to bacteriophage lambda. Proc Natl Acad Sci U S A (1975) 9.28

DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res (1992) 8.86

Polarity of Tn5 insertion mutations in Escherichia coli. J Bacteriol (1980) 7.55

Helicobacter pylori adhesin binding fucosylated histo-blood group antigens revealed by retagging. Science (1998) 6.99

Kinetic and genetic analyses of D-cycloserine inhibition and resistance in Escherichia coli. J Bacteriol (1965) 5.88

Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome. J Mol Biol (1971) 5.50

Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5. Genetics (1979) 5.49

Analyses of the cag pathogenicity island of Helicobacter pylori. Mol Microbiol (1998) 5.07

Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. The Tuberculosis Working Group in Peru. J Clin Microbiol (2000) 5.01

Recombination and clonal groupings within Helicobacter pylori from different geographical regions. Mol Microbiol (1999) 4.72

Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics (1967) 4.39

Nomenclature of transposable elements in prokaryotes. Gene (1979) 4.13

Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing. Infect Immun (1996) 3.89

Metronidazole resistance in Helicobacter pylori is due to null mutations in a gene (rdxA) that encodes an oxygen-insensitive NADPH nitroreductase. Mol Microbiol (1998) 3.44

Differences in genotypes of Helicobacter pylori from different human populations. J Bacteriol (2000) 3.39

Emergence of recombinant strains of Helicobacter pylori during human infection. Mol Microbiol (1999) 3.25

RAPD (arbitrary primer) PCR is more sensitive than multilocus enzyme electrophoresis for distinguishing related bacterial strains. Nucleic Acids Res (1993) 3.10

Specificity of transposon Tn5 insertion. Genetics (1983) 3.04

Epithelial attachment alters the outcome of Helicobacter pylori infection. Proc Natl Acad Sci U S A (1998) 2.96

PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori. Proc Natl Acad Sci U S A (1998) 2.93

Ordered cosmid library and high-resolution physical-genetic map of Helicobacter pylori strain NCTC11638. Mol Microbiol (1994) 2.93

Long-term colonization with single and multiple strains of Helicobacter pylori assessed by DNA fingerprinting. J Clin Microbiol (1995) 2.88

Distinctiveness of genotypes of Helicobacter pylori in Calcutta, India. J Bacteriol (2000) 2.81

PCR-based RFLP analysis of DNA sequence diversity in the gastric pathogen Helicobacter pylori. Nucleic Acids Res (1992) 2.62

Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5. Proc Natl Acad Sci U S A (1981) 2.59

Helicobacter pylori genetic diversity and risk of human disease. J Clin Invest (2001) 2.58

Diversity among clinical isolates of Histoplasma capsulatum detected by polymerase chain reaction with arbitrary primers. J Bacteriol (1992) 2.54

Restriction enzyme cleavage map of Tn10, a transposon which encodes tetracycline resistance. J Bacteriol (1979) 2.51

Insertion, excision, and inversion of Tn5. Cold Spring Harb Symp Quant Biol (1981) 2.37

Tn5tac1, a derivative of transposon Tn5 that generates conditional mutations. Proc Natl Acad Sci U S A (1988) 2.34

Did the inheritance of a pathogenicity island modify the virulence of Helicobacter pylori? Trends Microbiol (1997) 2.33

IS50-mediated inverse transposition. Discrimination between the two ends of an IS element. J Mol Biol (1982) 2.26

Nomenclature of transposable elements in prokaryotes. Plasmid (1979) 2.17

Rapid sequencing of cloned DNA using a transposon for bidirectional priming: sequence of the Escherichia coli K-12 avtA gene. Nucleic Acids Res (1987) 2.17

Prevalence of vacuolating cytotoxin production and distribution of distinct vacA alleles in Helicobacter pylori from China. J Infect Dis (1998) 2.09

Physiological characterization of polar Tn5-induced isoleucine-valine auxotrophs in Escherichia coli K.12: evidence for an internal promoter in the ilvOGEDA operon. Genetics (1979) 2.07

Host specificity of Helicobacter pylori strains and host responses in experimentally challenged nonhuman primates. Gastroenterology (1999) 1.96

Sequences essential for transposition at the termini of IS50. Proc Natl Acad Sci U S A (1983) 1.96

Structural requirement for IS50-mediated gene transposition. Proc Natl Acad Sci U S A (1983) 1.96

Bidirectional chain-termination nucleotide sequencing: transposon Tn5seq1 as a mobile source of primer sites. Gene (1988) 1.95

Salmonella typhimurium mutants defective in acetohydroxy acid synthases I and II. J Bacteriol (1980) 1.87

cysQ, a gene needed for cysteine synthesis in Escherichia coli K-12 only during aerobic growth. J Bacteriol (1992) 1.86

Metronidazole activation is mutagenic and causes DNA fragmentation in Helicobacter pylori and in Escherichia coli containing a cloned H. pylori RdxA(+) (Nitroreductase) gene. J Bacteriol (2000) 1.83

Transient and persistent experimental infection of nonhuman primates with Helicobacter pylori: implications for human disease. Infect Immun (1996) 1.81

Comparison of arbitrarily primed PCR and macrorestriction (pulsed-field gel electrophoresis) typing of Pseudomonas aeruginosa strains from cystic fibrosis patients. J Clin Microbiol (1995) 1.79

Sequential inactivation of rdxA (HP0954) and frxA (HP0642) nitroreductase genes causes moderate and high-level metronidazole resistance in Helicobacter pylori. J Bacteriol (2000) 1.77

Genetic evidence for two types of gene arrangements in new lambdadv plasmid mutants. J Mol Biol (1974) 1.76

Characterization of VPI pathogenicity island and CTXphi prophage in environmental strains of Vibrio cholerae. J Bacteriol (2001) 1.76

Analysis of an avtA::Mu d1(Ap lac) mutant: metabolic role of transaminase C. J Bacteriol (1982) 1.71

Novel sequence organization and insertion specificity of IS605 and IS606: chimaeric transposable elements of Helicobacter pylori. Gene (1998) 1.71

Identification of base pairs in the outside end of insertion sequence IS50 that are needed for IS50 and Tn5 transposition. Proc Natl Acad Sci U S A (1987) 1.70

Direct and crossover PCR amplification to facilitate Tn5supF-based sequencing of lambda phage clones. Nucleic Acids Res (1991) 1.68

Local DNA sequence control of deletion formation in Escherichia coli plasmid pBR322. Genetics (1987) 1.67

Factors affecting transposition activity of IS50 and Tn5 ends. Gene (1989) 1.67

Inverted repeats of Tn5 are transposable elements. Proc Natl Acad Sci U S A (1982) 1.64

Chromosome replication in some strains of Escherichia coli K12. Cold Spring Harb Symp Quant Biol (1968) 1.64

Use of arbitrarily primed polymerase chain reaction analysis to type disease and carrier strains of Neisseria meningitidis isolated during a university outbreak. J Infect Dis (1994) 1.63

Formation of supercoiling domains in plasmid pBR322. J Bacteriol (1989) 1.62

The gene for a tRNA modifying enzyme, m5U54-methyltransferase, is essential for viability in Escherichia coli. Proc Natl Acad Sci U S A (1992) 1.61

Tests of reciprocality in crossingover in partially diploid F strains of Escherichia coli. Genetics (1971) 1.60

Efficient introduction of cloned mutant alleles into the Escherichia coli chromosome. J Bacteriol (1991) 1.58

Absence of DNA sequences homologous to transposable element Tn5 (Kan) in the chromosome of Escherichia coli K-12. J Bacteriol (1978) 1.49

Localization of Helicobacter pylori urease and heat shock protein in human gastric biopsies. Infect Immun (1997) 1.49

Partial purification and some properties of delta1-pyrroline-5-carboxylate reductase from Escherichia coli. J Bacteriol (1977) 1.49

Insertion of mini-IS605 and deletion of adjacent sequences in the nitroreductase (rdxA) gene cause metronidazole resistance in Helicobacter pylori NCTC11637. Antimicrob Agents Chemother (1999) 1.48

Linear amplification DNA sequencing directly from single phage plaques and bacterial colonies. Nucleic Acids Res (1991) 1.44

Adaptive mutation and cocolonization during Helicobacter pylori infection of gnotobiotic piglets. Infect Immun (1995) 1.43

Proline excretion and indirect suppression in Escherichia coli and Salmonella typhimurium. J Bacteriol (1974) 1.41

Transposon Tn5 target specificity: preference for insertion at G/C pairs. Genetics (1988) 1.41

Cell vacuolation, a manifestation of the El tor hemolysin of Vibrio cholerae. Infect Immun (2000) 1.41

Cell vacuolation caused by Vibrio cholerae hemolysin. Infect Immun (2001) 1.40

The ilvG gene is expressed in Escherichia coli K-12. Gene (1980) 1.40

Roles of FrxA and RdxA nitroreductases of Helicobacter pylori in susceptibility and resistance to metronidazole. J Bacteriol (2001) 1.39

Differential recovery of auxotrophs after penicillin enrichment in Escherichia coli. J Bacteriol (1971) 1.39

Fast DNA isolation from Histoplasma capsulatum: methodology for arbitrary primer polymerase chain reaction-based epidemiological and clinical studies. J Clin Microbiol (1993) 1.38

Role of Helicobacter pylori cag region genes in colonization and gastritis in two animal models. Infect Immun (2001) 1.33

Chromosome replication in Escherichia coli. II. Origin of replication in F- and F+ strains. J Mol Biol (1969) 1.32

Functional organization and insertion specificity of IS607, a chimeric element of Helicobacter pylori. J Bacteriol (2000) 1.31

Arbitrarily primed PCR DNA fingerprinting of Escherichia coli O157:H7 strains by using templates from boiled cultures. J Clin Microbiol (1995) 1.31

Probe mapping to facilitate transposon-based DNA sequencing. Proc Natl Acad Sci U S A (1990) 1.29

Auxotroph accumulation in deoxyribonucleic acid polymeraseless strains of Escherichia coli K-12. J Bacteriol (1971) 1.29

Functional analysis of the ffh-trmD region of the Escherichia coli chromosome by using reverse genetics. J Bacteriol (1995) 1.28

Cloning and characterization of the Escherichia coli K-12 alanine-valine transaminase (avtA) gene. J Bacteriol (1987) 1.28

Genetic diversity of Helicobacter pylori. Lancet (1996) 1.26

Palindromy and the location of deletion endpoints in Escherichia coli. Genetics (1989) 1.26

Histidine-based zinc-binding sequences and the antimicrobial activity of calprotectin. J Infect Dis (1998) 1.26

DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting. J Bacteriol (1994) 1.24

Transfer of the lambda dv plasmid to new bacterial hosts. Virology (1974) 1.23

Identification of virulence genes of Helicobacter pylori by random insertion mutagenesis. Infect Immun (1999) 1.23

Genes of phage lambda essential for lambda dv plasmids. Virology (1974) 1.23

Organization and regulation of the ilvGEDA operon in Salmonella typhimurium LT2. J Bacteriol (1981) 1.22

Tn5supF, a 264-base-pair transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda. Proc Natl Acad Sci U S A (1989) 1.22

A derivative of Tn5 with direct terminal repeats can transpose. J Mol Biol (1982) 1.21

Role of alanine-valine transaminase in Salmonella typhimurium and analysis of an avtA::Tn5 mutant. J Bacteriol (1983) 1.20

Distribution of distinct vacA, cagA and iceA alleles in Helicobacter pylori in Hong Kong. Helicobacter (2001) 1.20