Published in Biotechnol Adv on October 08, 2011
Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines. Proc Natl Acad Sci U S A (2013) 1.17
In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation. Mol Syst Biol (2013) 1.05
In vitro evolution of α-hemolysin using a liposome display. Proc Natl Acad Sci U S A (2013) 1.00
Multiplexed in vivo His-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis. ACS Synth Biol (2012) 1.00
High-throughput cloning and expression library creation for functional proteomics. Proteomics (2013) 0.96
Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation. ACS Synth Biol (2014) 0.96
High-throughput identification of proteins with AMPylation using self-assembled human protein (NAPPA) microarrays. Nat Protoc (2015) 0.95
Cell-free metabolic engineering: biomanufacturing beyond the cell. Biotechnol J (2014) 0.94
CANDO and the infinite drug discovery frontier. Drug Discov Today (2014) 0.94
Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry. Curr Opin Biotechnol (2012) 0.93
Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis. Front Chem (2014) 0.92
IRES-mediated translation of membrane proteins and glycoproteins in eukaryotic cell-free systems. PLoS One (2013) 0.90
Synthetic in vitro circuits. Curr Opin Chem Biol (2012) 0.89
Bridging the gap: a roadmap to breaking the biological design barrier. Front Bioeng Biotechnol (2015) 0.89
Modulation of Intrinsically Disordered Protein Function by Post-translational Modifications. J Biol Chem (2016) 0.89
Engine out of the chassis: cell-free protein synthesis and its uses. FEBS Lett (2013) 0.88
Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1. Chembiochem (2015) 0.86
High-throughput preparation methods of crude extract for robust cell-free protein synthesis. Sci Rep (2015) 0.85
Robust production of recombinant phosphoproteins using cell-free protein synthesis. Nat Commun (2015) 0.84
Cell-free expression of functional receptor tyrosine kinases. Sci Rep (2015) 0.84
Development of a full-length human protein production pipeline. Protein Sci (2014) 0.84
Emerging technology of in situ cell free expression protein microarrays. Protein Cell (2012) 0.84
Engineering bacterial transcription regulation to create a synthetic in vitro two-hybrid system for protein interaction assays. J Am Chem Soc (2014) 0.83
Engineering artificial cells by combining HeLa-based cell-free expression and ultrathin double emulsion template. Methods Cell Biol (2015) 0.83
Host-pathogen interaction profiling using self-assembling human protein arrays. J Proteome Res (2015) 0.82
The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction. Nucleic Acids Res (2014) 0.82
Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems. Chembiochem (2015) 0.81
Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system. BMC Biotechnol (2014) 0.80
Repurposing the translation apparatus for synthetic biology. Curr Opin Chem Biol (2015) 0.80
Engineering genetic circuit interactions within and between synthetic minimal cells. Nat Chem (2016) 0.80
Wheat germ-based protein libraries for the functional characterisation of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase enzymes. BMC Plant Biol (2015) 0.79
A convenient and general expression platform for the production of secreted proteins from human cells. J Vis Exp (2012) 0.79
Cell-free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments. Eng Life Sci (2014) 0.79
DNA compaction induced by a cationic polymer or surfactant impact gene expression and DNA degradation. PLoS One (2014) 0.78
Induction of Gsk3β-β-TrCP interaction is required for late phase stabilization of β-catenin in canonical Wnt signaling. J Biol Chem (2014) 0.78
Cell-free translation and purification of Arabidopsis thaliana regulator of G signaling 1 protein. Protein Expr Purif (2016) 0.78
Protein aggregation with poly(vinyl) alcohol surfactant reduces double emulsion-encapsulated mammalian cell-free expression. PLoS One (2017) 0.77
Biochemical Preparation of Cell Extract for Cell-Free Protein Synthesis without Physical Disruption. PLoS One (2016) 0.77
Thrombin-mediated transcriptional regulation using DNA aptamers in DNA-based cell-free protein synthesis. ACS Synth Biol (2013) 0.77
Overview of cell-free protein synthesis: historic landmarks, commercial systems, and expanding applications. Curr Protoc Mol Biol (2014) 0.77
In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis. ACS Synth Biol (2016) 0.76
Energizing eukaryotic cell-free protein synthesis with glucose metabolism. FEBS Lett (2015) 0.76
A simplified and robust protocol for immunoglobulin expression in Escherichia coli cell-free protein synthesis systems. Biotechnol Prog (2015) 0.76
Cell-free synthesis of isotopically labelled peptide ligands for the functional characterization of G protein-coupled receptors. FEBS Open Bio (2015) 0.75
pH responsive granulocyte colony-stimulating factor variants with implications for treating Alzheimer's disease and other central nervous system disorders. Protein Eng Des Sel (2015) 0.75
Artificial Cells: Synthetic Compartments with Life-like Functionality and Adaptivity. Acc Chem Res (2017) 0.75
Personalised proteome analysis by means of protein microarrays made from individual patient samples. Sci Rep (2017) 0.75
A Simple and Rapid Method for Preparing a Cell-Free Bacterial Lysate for Protein Synthesis. PLoS One (2016) 0.75
A Novel Method to Evaluate Ribosomal Performance in Cell-Free Protein Synthesis Systems. Sci Rep (2017) 0.75
Cell-Free Systems Based on CHO Cell Lysates: Optimization Strategies, Synthesis of "Difficult-to-Express" Proteins and Future Perspectives. PLoS One (2016) 0.75
Protein synthesis using a reconstituted cell-free system. Curr Protoc Mol Biol (2014) 0.75
Cell-Free Expression and In Situ Immobilization of Parasite Proteins from Clonorchis sinensis for Rapid Identification of Antigenic Candidates. PLoS One (2015) 0.75
Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System. Biomed Res Int (2016) 0.75
Reaction dynamics analysis of a reconstituted Escherichia coli protein translation system by computational modeling. Proc Natl Acad Sci U S A (2017) 0.75
Overview of the purification of recombinant proteins. Curr Protoc Protein Sci (2015) 0.75
Synthetic biology approaches to biological containment: pre-emptively tackling potential risks. Essays Biochem (2016) 0.75
In vitro membrane protein synthesis inside Sec translocon-reconstituted cell-sized liposomes. Sci Rep (2016) 0.75
Biomolecular engineering for nanobio/bionanotechnology. Nano Converg (2017) 0.75
Cell-sized mechanosensitive and biosensing compartment programmed with DNA. Chem Commun (Camb) (2017) 0.75
Dissecting limiting factors of the Protein synthesis Using Recombinant Elements (PURE) system. Translation (Austin) (2017) 0.75
High-yield production of "difficult-to-express" proteins in a continuous exchange cell-free system based on CHO cell lysates. Sci Rep (2017) 0.75
Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates. Sci Rep (2017) 0.75
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Human protein factory for converting the transcriptome into an in vitro-expressed proteome,. Nat Methods (2008) 1.88
Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. Infect Immun (2008) 1.87
The coming of age of virus-like particle vaccines. Biol Chem (2008) 1.85
An integrated cell-free metabolic platform for protein production and synthetic biology. Mol Syst Biol (2008) 1.72
Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target. Nat Methods (2007) 1.68
Bridging IRES elements in mRNAs to the eukaryotic translation apparatus. Biochim Biophys Acta (2009) 1.62
A cell-free protein-producing gel. Nat Mater (2009) 1.59
Cell-free expression systems for eukaryotic protein production. Curr Opin Biotechnol (2006) 1.58
Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis. Biotechnol Bioeng (2004) 1.56
The past, present and future of cell-free protein synthesis. Trends Biotechnol (2005) 1.51
Practical cell-free protein synthesis system using purified wheat embryos. Nat Protoc (2010) 1.47
The 5'-leader of tobacco mosaic virus promotes translation through enhanced recruitment of eIF4F. Nucleic Acids Res (2002) 1.40
Microscale to manufacturing scale-up of cell-free cytokine production--a new approach for shortening protein production development timelines. Biotechnol Bioeng (2011) 1.36
Regeneration of adenosine triphosphate from glycolytic intermediates for cell-free protein synthesis. Biotechnol Bioeng (2001) 1.29
A cell free system from HeLa cells active in initiation of protein synthesis. Biochemistry (1975) 1.28
Species-independent translational leaders facilitate cell-free expression. Nat Biotechnol (2009) 1.22
Developing cell-free biology for industrial applications. J Ind Microbiol Biotechnol (2006) 1.17
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De novo folding of GFP fusion proteins: high efficiency in eukaryotes but not in bacteria. J Mol Biol (2005) 1.15
In vitro evolution of single-chain antibodies using mRNA display. Nucleic Acids Res (2006) 1.11
Towards the preparative and large-scale precision manufacture of virus-like particles. Trends Biotechnol (2005) 1.11
Cell-free synthesis and maturation of [FeFe] hydrogenases. Biotechnol Bioeng (2008) 1.11
Cell-free production of scFv fusion proteins: an efficient approach for personalized lymphoma vaccines. Blood (2006) 1.11
Development of cell-free protein synthesis platforms for disulfide bonded proteins. Biotechnol Bioeng (2008) 1.10
Cell-free translation of integral membrane proteins into unilamelar liposomes. Methods Enzymol (2009) 1.10
The wheat germ cell-free protein synthesis system: a key tool for novel malaria vaccine candidate discovery. Acta Trop (2009) 1.09
Efficient production of a bioactive, multiple disulfide-bonded protein using modified extracts of Escherichia coli. Biotechnol Bioeng (2004) 1.06
Total amino acid stabilization during cell-free protein synthesis reactions. J Biotechnol (2006) 1.06
Streamlining Escherichia coli S30 extract preparation for economical cell-free protein synthesis. Biotechnol Prog (2005) 1.05
High yield cell-free production of integral membrane proteins without refolding or detergents. Biochim Biophys Acta (2008) 1.04
Prolonging cell-free protein synthesis with a novel ATP regeneration system. Biotechnol Bioeng (1999) 1.04
An economical method for cell-free protein synthesis using glucose and nucleoside monophosphates. Biotechnol Prog (2005) 1.04
Substrate replenishment extends protein synthesis with an in vitro translation system designed to mimic the cytoplasm. Biotechnol Bioeng (2004) 1.04
Energizing cell-free protein synthesis with glucose metabolism. Biotechnol Bioeng (2005) 0.99
Rapid expression of vaccine proteins for B-cell lymphoma in a cell-free system. Biotechnol Bioeng (2005) 0.99
Escherichia coli-based cell-free synthesis of virus-like particles. Biotechnol Bioeng (2008) 0.98
Enhancing multiple disulfide bonded protein folding in a cell-free system. Biotechnol Bioeng (2004) 0.98
Efficacy of a potential trivalent vaccine based on Hc fragments of botulinum toxins A, B, and E produced in a cell-free expression system. Clin Vaccine Immunol (2010) 0.97
In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme. Nucleic Acids Res (2010) 0.97
Amino acid stabilization for cell-free protein synthesis by modification of the Escherichia coli genome. Metab Eng (2004) 0.97
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A hybridoma-based in vitro translation system that efficiently synthesizes glycoproteins. J Biotechnol (2006) 0.96
Maintaining rapid growth in moderate-density Escherichia coli fermentations. Biotechnol Bioeng (2005) 0.96
Development of an in vitro compartmentalization screen for high-throughput directed evolution of [FeFe] hydrogenases. PLoS One (2010) 0.95
Surface functionalization of virus-like particles by direct conjugation using azide-alkyne click chemistry. Bioconjug Chem (2011) 0.95
In situ synthesis of protein arrays. Curr Opin Biotechnol (2008) 0.95
Performance of expression vector, pTD1, in insect cell-free translation system. J Biosci Bioeng (2006) 0.95
Cell-free expression for nanolipoprotein particles: building a high-throughput membrane protein solubility platform. Methods Mol Biol (2009) 0.93
High-throughput, genome-scale protein production method based on the wheat germ cell-free expression system. Biotechnol Adv (2003) 0.92
Prolonging cell-free protein synthesis by selective reagent additions. Biotechnol Prog (2000) 0.92
A wheat germ cell-free system is a novel way to screen protein folding and function. Protein Sci (2003) 0.91
A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based. BMC Biotechnol (2008) 0.89
A highly controllable reconstituted cell-free system--a breakthrough in protein synthesis research. Curr Pharm Biotechnol (2010) 0.89
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Cell-free protein synthesis energized by slowly-metabolized maltodextrin. BMC Biotechnol (2009) 0.88
Label-free, single protein detection on a near-infrared fluorescent single-walled carbon nanotube/protein microarray fabricated by cell-free synthesis. Nano Lett (2011) 0.87
A sequential expression system for high-throughput functional genomic analysis. Proteomics (2007) 0.86
Efficient disulfide bond formation in virus-like particles. J Biotechnol (2011) 0.85
In vitro selection of zinc finger DNA-binding proteins through ribosome display. Biochem Biophys Res Commun (2006) 0.85
Cell-free expression of soluble and membrane proteins in an array device for drug screening. Anal Chem (2010) 0.84
Rapid discovery of protein interactions by cell-free protein technologies. Biochem Soc Trans (2007) 0.84
Cell-free synthesis of 15N-labeled proteins for NMR studies. IUBMB Life (2005) 0.84
Universal cell-free protein synthesis. Nat Biotechnol (2009) 0.84
Protein prenylation in an insect cell-free protein synthesis system and identification of products by mass spectrometry. Proteomics (2007) 0.84
A cell-free protein synthesis system from insect cells. Methods Mol Biol (2010) 0.84
Expression-independent consumption of substrates in cell-free expression system from Escherichia coli. J Biotechnol (2001) 0.83
Differential post-translational modification of human type I keratins synthesized in a rabbit reticulocyte cell-free system. Biochim Biophys Acta (1985) 0.82
Establishment and characterization of cell-free translation/glycosylation in insect cell (Spodoptera frugiperda 21) extract prepared with high pressure treatment. Appl Microbiol Biotechnol (2001) 0.82
Amphiphilic polysaccharide nanogels as artificial chaperones in cell-free protein synthesis. Macromol Biosci (2011) 0.82
Localization of BiP to translating ribosomes increases soluble accumulation of secreted eukaryotic proteins in an Escherichia coli cell-free system. Biotechnol Bioeng (2011) 0.82
Chapter 2. Development of key technologies for high-throughput cell-free protein production with the extract from wheat embryos. Adv Protein Chem Struct Biol (2009) 0.81
Cell-free protein synthesis using multiply-primed rolling circle amplification products. Biotechniques (2009) 0.81
Rapid expression of functional genomic libraries. J Proteome Res (2006) 0.80
Ribosome-display technology: applications for directed evolution of functional proteins. Drug Discov Today (2006) 0.79
N-Terminal protein modifications in an insect cell-free protein synthesis system and their identification by mass spectrometry. Proteomics (2006) 0.78
Preparation of ubiquitin-conjugated proteins using an insect cell-free protein synthesis system. J Biotechnol (2010) 0.77
Advocating for the quadravalent HPV vaccination, Gardasil, by Merck. Int J Pediatr Otorhinolaryngol (2007) 0.77
Precise manipulation of chromosomes in vivo enables genome-wide codon replacement. Science (2011) 3.49
Linking high-resolution metabolic flux phenotypes and transcriptional regulation in yeast modulated by the global regulator Gcn4p. Proc Natl Acad Sci U S A (2009) 1.79
The genome-scale metabolic model iIN800 of Saccharomyces cerevisiae and its validation: a scaffold to query lipid metabolism. BMC Syst Biol (2008) 1.73
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A combined cell-free transcription-translation system from Saccharomyces cerevisiae for rapid and robust protein synthe. Biotechnol J (2014) 1.41
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Growth temperature exerts differential physiological and transcriptional responses in laboratory and wine strains of Saccharomyces cerevisiae. Appl Environ Microbiol (2008) 1.06
Multiplexed in vivo His-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis. ACS Synth Biol (2012) 1.00
Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation. ACS Synth Biol (2014) 0.96
Cell-free metabolic engineering: biomanufacturing beyond the cell. Biotechnol J (2014) 0.94
Identification and functional characterization of a leucine-rich repeat receptor-like kinase gene that is involved in regulation of soybean leaf senescence. Plant Mol Biol (2006) 0.94
Cell-free protein synthesis with prokaryotic combined transcription-translation. Methods Mol Biol (2004) 0.93
Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry. Curr Opin Biotechnol (2012) 0.93
Synthetic in vitro circuits. Curr Opin Chem Biol (2012) 0.89
Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1. Chembiochem (2015) 0.86
Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis. Biotechnol Bioeng (2013) 0.81
Biology by design: from top to bottom and back. J Biomed Biotechnol (2010) 0.78
Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis. Biotechnol J (2014) 0.77
Enabling a next generation of synthetic biology community organization and leadership. ACS Synth Biol (2014) 0.75
Nanobiotechnology: synthetic biology meets materials science. Curr Opin Biotechnol (2013) 0.75
[Biological functions of leucine-rich repeat class of receptor-like protein kinases in plants]. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao (2005) 0.75
Cloning and characterization of a novel soybean receptor-like protein kinase gene GmRLCK. Fen Zi Xi Bao Sheng Wu Xue Bao (2006) 0.75
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