Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury.

PubWeight™: 2.65‹?› | Rank: Top 1%

🔗 View Article (PMID 22198277)

Published in Nat Med on December 25, 2011

Authors

Ali Ertürk1, Christoph P Mauch, Farida Hellal, Friedrich Förstner, Tara Keck, Klaus Becker, Nina Jährling, Heinz Steffens, Melanie Richter, Mark Hübener, Edgar Kramer, Frank Kirchhoff, Hans Ulrich Dodt, Frank Bradke

Author Affiliations

1: Max Planck Institute of Neurobiology, Axonal Growth and Regeneration, Martinsried, Germany.

Articles citing this

Structural and molecular interrogation of intact biological systems. Nature (2013) 8.95

SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction. Nat Neurosci (2013) 3.69

CLARITY for mapping the nervous system. Nat Methods (2013) 3.20

Three-dimensional imaging of solvent-cleared organs using 3DISCO. Nat Protoc (2012) 3.19

Nerve regeneration restores supraspinal control of bladder function after complete spinal cord injury. J Neurosci (2013) 2.43

ClearT: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue. Development (2013) 2.33

Chemical clearing and dehydration of GFP expressing mouse brains. PLoS One (2012) 2.19

Clarifying Tissue Clearing. Cell (2015) 2.05

Functional regeneration beyond the glial scar. Exp Neurol (2014) 1.79

Concepts and methods for the study of axonal regeneration in the CNS. Neuron (2012) 1.74

Live imaging of astrocyte responses to acute injury reveals selective juxtavascular proliferation. Nat Neurosci (2013) 1.62

Recent progress in tissue optical clearing. Laser Photon Rev (2013) 1.46

Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping. Nat Protoc (2015) 1.25

Misguidance and modulation of axonal regeneration by Stat3 and Rho/ROCK signaling in the transparent optic nerve. Cell Death Dis (2013) 1.23

Three-dimensional evaluation of retinal ganglion cell axon regeneration and pathfinding in whole mouse tissue after injury. Exp Neurol (2013) 1.23

Fluorescent-protein stabilization and high-resolution imaging of cleared, intact mouse brains. PLoS One (2015) 1.05

Multispectral fluorescence ultramicroscopy: three-dimensional visualization and automatic quantification of tumor morphology, drug penetration, and antiangiogenic treatment response. Neoplasia (2014) 1.03

Shrinkage-mediated imaging of entire organs and organisms using uDISCO. Nat Methods (2016) 0.98

Optical histology: a method to visualize microvasculature in thick tissue sections of mouse brain. PLoS One (2013) 0.98

Quantitative neuroanatomy of all Purkinje cells with light sheet microscopy and high-throughput image analysis. Front Neuroanat (2015) 0.96

Scalable and DiI-compatible optical clearance of the mammalian brain. Front Neuroanat (2015) 0.96

Comprehensive evaluation of peripheral nerve regeneration in the acute healing phase using tissue clearing and optical microscopy in a rodent model. PLoS One (2014) 0.95

A rapid optical clearing protocol using 2,2'-thiodiethanol for microscopic observation of fixed mouse brain. PLoS One (2015) 0.94

Ex vivo fluorescence molecular tomography of the spine. Int J Biomed Imaging (2012) 0.92

A method for 3D immunostaining and optical imaging of the mouse brain demonstrated in neural progenitor cells. PLoS One (2013) 0.90

Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging. Physiol Genomics (2012) 0.90

Molecular psychiatry of zebrafish. Mol Psychiatry (2014) 0.89

Application and assessment of optical clearing methods for imaging of tissue-engineered neural stem cell spheres. Tissue Eng Part C Methods (2014) 0.88

A method for the three-dimensional reconstruction of Neurobiotin™-filled neurons and the location of their synaptic inputs. Front Neural Circuits (2013) 0.87

Imaging cleared intact biological systems at a cellular level by 3DISCO. J Vis Exp (2014) 0.87

In-vivo optical tomography of small scattering specimens: time-lapse 3D imaging of the head eversion process in Drosophila melanogaster. Sci Rep (2014) 0.86

Viral vector-based improvement of optic nerve regeneration: characterization of individual axons' growth patterns and synaptogenesis in a visual target. Gene Ther (2015) 0.86

Extracting structural and functional features of widely distributed biological circuits with single cell resolution via tissue clearing and delivery vectors. Curr Opin Biotechnol (2016) 0.86

Abrogation of β-catenin signaling in oligodendrocyte precursor cells reduces glial scarring and promotes axon regeneration after CNS injury. J Neurosci (2014) 0.85

3D Imaging of Axons in Transparent Spinal Cords from Rodents and Nonhuman Primates(,.) eNeuro (2015) 0.84

Deep tissue imaging: a review from a preclinical cancer research perspective. Histochem Cell Biol (2016) 0.84

Trans-nodal migration of resident dendritic cells into medullary interfollicular regions initiates immunity to influenza vaccine. J Exp Med (2014) 0.83

Bringing CLARITY to gray matter atrophy. Neuroimage (2014) 0.82

Metallothionein-I/II Promotes Axonal Regeneration in the Central Nervous System. J Biol Chem (2015) 0.82

Mapping immune processes in intact tissues at cellular resolution. J Clin Invest (2012) 0.80

Optical clearing in dense connective tissues to visualize cellular connectivity in situ. PLoS One (2015) 0.80

Correlated magnetic resonance imaging and ultramicroscopy (MR-UM) is a tool kit to assess the dynamics of glioma angiogenesis. Elife (2016) 0.79

High-performance multiplexed fluorescence in situ hybridization in culture and tissue with matrix imprinting and clearing. Proc Natl Acad Sci U S A (2016) 0.79

High-resolution live imaging reveals axon-glia interactions during peripheral nerve injury and repair in zebrafish. Dis Model Mech (2015) 0.79

Optical properties of mouse brain tissue after optical clearing with FocusClear™. J Biomed Opt (2015) 0.79

Optical Coherence Tomography for Brain Imaging and Developmental Biology. IEEE J Sel Top Quantum Electron (2015) 0.78

Cerebral β-Amyloidosis in Mice Investigated by Ultramicroscopy. PLoS One (2015) 0.78

Optical reconstruction of murine colorectal mucosa at cellular resolution. Am J Physiol Gastrointest Liver Physiol (2015) 0.77

Ultramicroscopy: development and outlook. Neurophotonics (2015) 0.77

Sleep as a biological problem: an overview of frontiers in sleep research. J Physiol Sci (2015) 0.77

Nonspecific labeling limits the utility of Cre-Lox bred CST-YFP mice for studies of corticospinal tract regeneration. J Comp Neurol (2015) 0.77

Visible rodent brain-wide networks at single-neuron resolution. Front Neuroanat (2015) 0.77

Clearing and Labeling Techniques for Large-Scale Biological Tissues. Mol Cells (2016) 0.76

Dual-slit confocal light sheet microscopy for in vivo whole-brain imaging of zebrafish. Biomed Opt Express (2015) 0.76

Inhibition of Poly-ADP-Ribosylation Fails to Increase Axonal Regeneration or Improve Functional Recovery after Adult Mammalian CNS Injury. eNeuro (2016) 0.76

Whole-mount imaging of the mouse hindlimb vasculature using the lipophilic carbocyanine dye DiI. Biotechniques (2012) 0.76

High-resolution 3D imaging of whole organ after clearing: taking a new look at the zebrafish testis. Sci Rep (2017) 0.75

Whole-brain 3D mapping of human neural transplant innervation. Nat Commun (2017) 0.75

Elevated-temperature-induced acceleration of PACT clearing process of mouse brain tissue. Sci Rep (2017) 0.75

Adult stem cell lineage tracing and deep tissue imaging. BMB Rep (2015) 0.75

Between new genetic discoveries and large randomized trials--neurological research in the era of systems medicine. EMBO Rep (2013) 0.75

Three-dimensional histology: new visual approaches to morphological changes during neural regeneration. Neural Regen Res (2017) 0.75

A combined method for correlative 3D imaging of biological samples from macro to nano scale. Sci Rep (2016) 0.75

CLARITY-compatible lipophilic dyes for electrode marking and neuronal tracing. Sci Rep (2016) 0.75

Quantitative 3D investigation of Neuronal network in mouse spinal cord model. Sci Rep (2017) 0.75

Intravital imaging of axonal interactions with microglia and macrophages in a mouse dorsal column crush injury. J Vis Exp (2014) 0.75

A Combination of Ex vivo Diffusion MRI and Multiphoton to Study Microglia/Monocytes Alterations after Spinal Cord Injury. Front Aging Neurosci (2017) 0.75

Optic nerve regeneration in mammals: Regenerated or spared axons? Exp Neurol (2017) 0.75

3D visualization and quantification of microvessels in the whole ischemic mouse brain using solvent-based clearing and light sheet microscopy. J Cereb Blood Flow Metab (2017) 0.75

Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology? BMC Biol (2017) 0.75

Articles cited by this

Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron (2000) 22.02

Analysis of fractalkine receptor CX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertion. Mol Cell Biol (2000) 14.74

Regeneration beyond the glial scar. Nat Rev Neurosci (2004) 11.00

Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain. Nat Methods (2007) 8.32

Chondroitinase ABC promotes functional recovery after spinal cord injury. Nature (2002) 8.22

Reactive astrocytes protect tissue and preserve function after spinal cord injury. J Neurosci (2004) 5.70

Mosaic analysis with double markers in mice. Cell (2005) 5.32

Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature (2000) 5.22

Regeneration of dorsal column fibers into and beyond the lesion site following adult spinal cord injury. Neuron (1999) 4.13

In vivo imaging of axonal degeneration and regeneration in the injured spinal cord. Nat Med (2005) 3.88

Nogo-66 receptor antagonist peptide promotes axonal regeneration. Nature (2002) 3.33

Nogo-A-specific antibody treatment enhances sprouting and functional recovery after cervical lesion in adult primates. Nat Med (2006) 3.23

GFAP promoter-controlled EGFP-expressing transgenic mice: a tool to visualize astrocytes and astrogliosis in living brain tissue. Glia (2001) 3.20

Functional regeneration of sensory axons into the adult spinal cord. Nature (2000) 2.70

Disorganized microtubules underlie the formation of retraction bulbs and the failure of axonal regeneration. J Neurosci (2007) 2.51

False resurrections: distinguishing regenerated from spared axons in the injured central nervous system. J Comp Neurol (2003) 2.34

Ome sweet ome: what can the genome tell us about the connectome? Curr Opin Neurobiol (2008) 2.33

Transneuronal circuit tracing with neurotropic viruses. Curr Opin Neurobiol (2009) 2.26

One rule to grow them all: a general theory of neuronal branching and its practical application. PLoS Comput Biol (2010) 1.98

Chronically CNS-injured adult sensory neurons gain regenerative competence upon a lesion of their peripheral axon. Curr Biol (2009) 1.79

Axonal growth and guidance defects in Frizzled3 knock-out mice: a comparison of diffusion tensor magnetic resonance imaging, neurofilament staining, and genetically directed cell labeling. J Neurosci (2006) 1.64

Optimizing imaging parameters for the separation of multiple labels in a fluorescence image. J Microsc (2004) 1.62

Regenerating corticospinal fibers in the Marmoset (Callitrix jacchus) after spinal cord lesion and treatment with the anti-Nogo-A antibody IN-1. Eur J Neurosci (2004) 1.19

Electrical activity suppresses axon growth through Ca(v)1.2 channels in adult primary sensory neurons. Curr Biol (2010) 1.17

Glial and axonal responses in areas of Wallerian degeneration of the corticospinal and dorsal ascending tracts after spinal cord dorsal funiculotomy. Neuropathology (2008) 0.97

Preparation of a protein-free total brain white matter lipid fraction: characterization of liposomes. J Neurosci Res (1992) 0.85

Embedding, sectioning, immunocytochemical and stereological methods that optimise research on the lesioned adult rat spinal cord. J Neurosci Methods (1996) 0.85

Modulation of NADPH-diaphorase and glial fibrillary acidic protein by progesterone in astrocytes from normal and injured rat spinal cord. J Steroid Biochem Mol Biol (2000) 0.83

Articles by these authors

Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. Science (2005) 14.43

Lifeact: a versatile marker to visualize F-actin. Nat Methods (2008) 12.23

Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain. Nat Methods (2007) 8.32

Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods (2004) 6.59

Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Nat Protoc (2009) 5.68

Semen-derived amyloid fibrils drastically enhance HIV infection. Cell (2007) 5.23

A genetically encoded calcium indicator for chronic in vivo two-photon imaging. Nat Methods (2008) 4.13

Nef-mediated suppression of T cell activation was lost in a lentiviral lineage that gave rise to HIV-1. Cell (2006) 4.13

Nef proteins from simian immunodeficiency viruses are tetherin antagonists. Cell Host Microbe (2009) 4.11

Neuronal or glial progeny: regional differences in radial glia fate. Neuron (2003) 3.68

Experience leaves a lasting structural trace in cortical circuits. Nature (2008) 3.66

Tetherin-driven adaptation of Vpu and Nef function and the evolution of pandemic and nonpandemic HIV-1 strains. Cell Host Microbe (2009) 3.58

Microtubule stabilization reduces scarring and causes axon regeneration after spinal cord injury. Science (2011) 3.45

Homeostatic regulation of eye-specific responses in visual cortex during ocular dominance plasticity. Neuron (2007) 3.41

Three-dimensional imaging of solvent-cleared organs using 3DISCO. Nat Protoc (2012) 3.19

A role for glia in the progression of Rett's syndrome. Nature (2011) 3.18

Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J Agric Food Chem (2003) 3.12

The biological action of saponins in animal systems: a review. Br J Nutr (2002) 2.97

Highly ordered arrangement of single neurons in orientation pinwheels. Nature (2006) 2.91

Regeneration of sensory axons within the injured spinal cord induced by intraganglionic cAMP elevation. Neuron (2002) 2.84

Evolutionary and functional analyses of the interaction between the myeloid restriction factor SAMHD1 and the lentiviral Vpx protein. Cell Host Microbe (2012) 2.66

Viscoelastic properties of individual glial cells and neurons in the CNS. Proc Natl Acad Sci U S A (2006) 2.64

Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication. PLoS Biol (2013) 2.61

Microtubule stabilization specifies initial neuronal polarization. J Cell Biol (2008) 2.60

Disorganized microtubules underlie the formation of retraction bulbs and the failure of axonal regeneration. J Neurosci (2007) 2.51

Progeny of Olig2-expressing progenitors in the gray and white matter of the adult mouse cerebral cortex. J Neurosci (2008) 2.49

Microglia: new roles for the synaptic stripper. Neuron (2013) 2.44

Toward an AIDS vaccine: lessons from natural simian immunodeficiency virus infections of African nonhuman primate hosts. Nat Med (2009) 2.41

The cationic properties of SEVI underlie its ability to enhance human immunodeficiency virus infection. J Virol (2008) 2.38

Segregated expression of AMPA-type glutamate receptors and glutamate transporters defines distinct astrocyte populations in the mouse hippocampus. J Neurosci (2003) 2.29

Discovery and optimization of a natural HIV-1 entry inhibitor targeting the gp41 fusion peptide. Cell (2007) 2.22

Chemical clearing and dehydration of GFP expressing mouse brains. PLoS One (2012) 2.19

Massive restructuring of neuronal circuits during functional reorganization of adult visual cortex. Nat Neurosci (2008) 2.18

Locus ceruleus controls Alzheimer's disease pathology by modulating microglial functions through norepinephrine. Proc Natl Acad Sci U S A (2010) 2.15

Low levels of SIV infection in sooty mangabey central memory CD⁴⁺ T cells are associated with limited CCR5 expression. Nat Med (2011) 2.08

Down-modulation of mature major histocompatibility complex class II and up-regulation of invariant chain cell surface expression are well-conserved functions of human and simian immunodeficiency virus nef alleles. J Virol (2003) 2.02

Sensorimotor mismatch signals in primary visual cortex of the behaving mouse. Neuron (2012) 2.01

Fibrils of prostatic acid phosphatase fragments boost infections with XMRV (xenotropic murine leukemia virus-related virus), a human retrovirus associated with prostate cancer. J Virol (2009) 2.00

Lifeact mice for studying F-actin dynamics. Nat Methods (2010) 2.00

In vivo fate mapping and expression analysis reveals molecular hallmarks of prospectively isolated adult neural stem cells. Cell Stem Cell (2010) 1.95

Plasticity of polarization: changing dendrites into axons in neurons integrated in neuronal circuits. Curr Biol (2008) 1.95

Critical-period plasticity in the visual cortex. Annu Rev Neurosci (2012) 1.85

Nanoscopy in a living mouse brain. Science (2012) 1.80

Chronically CNS-injured adult sensory neurons gain regenerative competence upon a lesion of their peripheral axon. Curr Biol (2009) 1.79

Contribution of Vpu, Env, and Nef to CD4 down-modulation and resistance of human immunodeficiency virus type 1-infected T cells to superinfection. J Virol (2006) 1.77

Prior experience enhances plasticity in adult visual cortex. Nat Neurosci (2005) 1.76

Cdc42 regulates cofilin during the establishment of neuronal polarity. J Neurosci (2007) 1.73

In vivo imaging of intersynaptic vesicle exchange using VGLUT1 Venus knock-in mice. J Neurosci (2011) 1.73

Synaptic scaling and homeostatic plasticity in the mouse visual cortex in vivo. Neuron (2013) 1.73

Mapping retinotopic structure in mouse visual cortex with optical imaging. J Neurosci (2002) 1.72

Peptides released by physiological cleavage of semen coagulum proteins form amyloids that enhance HIV infection. Cell Host Microbe (2011) 1.72

Semen-mediated enhancement of HIV infection is donor-dependent and correlates with the levels of SEVI. Retrovirology (2010) 1.70

NMDA receptors mediate neuron-to-glia signaling in mouse cortical astrocytes. J Neurosci (2006) 1.69

Epilepsy in small-world networks. J Neurosci (2004) 1.69

Axon extension occurs independently of centrosomal microtubule nucleation. Science (2010) 1.63

Nef-mediated enhancement of virion infectivity and stimulation of viral replication are fundamental properties of primate lentiviruses. J Virol (2007) 1.58

3D-reconstruction of blood vessels by ultramicroscopy. Organogenesis (2009) 1.58

Lack of the Kir4.1 channel subunit abolishes K+ buffering properties of astrocytes in the ventral respiratory group: impact on extracellular K+ regulation. J Neurophysiol (2005) 1.57

Phorbol esters: structure, biological activity, and toxicity in animals. Int J Toxicol (2007) 1.56

A flow cytometry-based FRET assay to identify and analyse protein-protein interactions in living cells. PLoS One (2010) 1.54

Ancient origin of a deletion in human BST2/Tetherin that confers protection against viral zoonoses. Hum Mutat (2011) 1.54

Nef enhances human immunodeficiency virus type 1 infectivity and replication independently of viral coreceptor tropism. J Virol (2002) 1.53

Loss of sensory input causes rapid structural changes of inhibitory neurons in adult mouse visual cortex. Neuron (2011) 1.49

Astroglial processes show spontaneous motility at active synaptic terminals in situ. Eur J Neurosci (2004) 1.49

Rac1 regulates neuronal polarization through the WAVE complex. J Neurosci (2010) 1.49

The role of the cytoskeleton during neuronal polarization. Curr Opin Neurobiol (2008) 1.49

Neuronal polarity. Cold Spring Harb Perspect Biol (2009) 1.42

Seizures preferentially stimulate proliferation of radial glia-like astrocytes in the adult dentate gyrus: functional and immunocytochemical analysis. Eur J Neurosci (2003) 1.42

Expression of reef coral fluorescent proteins in the central nervous system of transgenic mice. Mol Cell Neurosci (2005) 1.42

Temporal control of gene recombination in astrocytes by transgenic expression of the tamoxifen-inducible DNA recombinase variant CreERT2. Glia (2006) 1.42

Naturally occurring fragments from two distinct regions of the prostatic acid phosphatase form amyloidogenic enhancers of HIV infection. J Virol (2011) 1.42

Control of neuronal polarity and plasticity--a renaissance for microtubules? Trends Cell Biol (2009) 1.40

Aminoquinoline surfen inhibits the action of SEVI (semen-derived enhancer of viral infection). J Biol Chem (2009) 1.39

Altered map of visual space in the superior colliculus of mice lacking early retinal waves. J Neurosci (2005) 1.38

LSECtin interacts with filovirus glycoproteins and the spike protein of SARS coronavirus. Virology (2005) 1.37

Netrin-1 is a novel myelin-associated inhibitor to axon growth. J Neurosci (2008) 1.36

NO mediates microglial response to acute spinal cord injury under ATP control in vivo. Glia (2010) 1.36

Reduced spine density in specific regions of CA1 pyramidal neurons in two transgenic mouse models of Alzheimer's disease. J Neurosci (2011) 1.36

Conserved residue Lys574 in the cavity of HIV-1 Gp41 coiled-coil domain is critical for six-helix bundle stability and virus entry. J Biol Chem (2007) 1.34

Circulating Nef induces dyslipidemia in simian immunodeficiency virus-infected macaques by suppressing cholesterol efflux. J Infect Dis (2010) 1.34

NMDA Receptors in glia. Neuroscientist (2007) 1.33

The DLK signalling pathway--a double-edged sword in neural development and regeneration. EMBO Rep (2013) 1.32

The EphA4 receptor regulates dendritic spine remodeling by affecting beta1-integrin signaling pathways. J Cell Biol (2007) 1.32

Nef induces multiple genes involved in cholesterol synthesis and uptake in human immunodeficiency virus type 1-infected T cells. J Virol (2005) 1.31

Human immunodeficiency virus type 1 inhibits DNA damage-triggered apoptosis by a Nef-independent mechanism. J Virol (2005) 1.29

Tetherin: holding on and letting go. Cell (2010) 1.29

Growth cone-like waves transport actin and promote axonogenesis and neurite branching. Dev Neurobiol (2009) 1.28

Reducing HDAC6 ameliorates cognitive deficits in a mouse model for Alzheimer's disease. EMBO Mol Med (2012) 1.28