Apo-intermediate in the transport cycle of lactose permease (LacY).

Crystal structure of a eukaryotic phosphate transporter. Nature (2013) 1.60

Observing a lipid-dependent alteration in single lactose permeases. Structure (2015) 1.48

Structure-based mechanism for Na(+)/melibiose symport by MelB. Nat Commun (2014) 1.25

Structural insights into substrate recognition in proton-dependent oligopeptide transporters. EMBO Rep (2013) 1.08

Evolutionary mix-and-match with MFS transporters II. Proc Natl Acad Sci U S A (2013) 1.05

Functional architecture of MFS D-glucose transporters. Proc Natl Acad Sci U S A (2014) 1.04

A chemiosmotic mechanism of symport. Proc Natl Acad Sci U S A (2015) 0.97

Protonation drives the conformational switch in the multidrug transporter LmrP. Nat Chem Biol (2013) 0.97

Gating topology of the proton-coupled oligopeptide symporters. Structure (2015) 0.93

Flexible gates generate occluded intermediates in the transport cycle of LacY. J Mol Biol (2014) 0.92

Identification of molecular hinge points mediating alternating access in the vesicular monoamine transporter VMAT2. Proc Natl Acad Sci U S A (2013) 0.91

Understanding transporter specificity and the discrete appearance of channel-like gating domains in transporters. Front Pharmacol (2014) 0.89

Trp replacements for tightly interacting Gly-Gly pairs in LacY stabilize an outward-facing conformation. Proc Natl Acad Sci U S A (2013) 0.86

Substrate-induced changes in the structural properties of LacY. Proc Natl Acad Sci U S A (2014) 0.86

Real-time conformational changes in LacY. Proc Natl Acad Sci U S A (2014) 0.85

Outward-facing conformers of LacY stabilized by nanobodies. Proc Natl Acad Sci U S A (2014) 0.81

Transient conformers of LacY are trapped by nanobodies. Proc Natl Acad Sci U S A (2015) 0.77

Mechanism of Substrate Translocation in an Alternating Access Transporter. Cell (2017) 0.77

UCSF Chimera--a visualization system for exploratory research and analysis. J Comput Chem (2004) 112.47

Comparative protein modelling by satisfaction of spatial restraints. J Mol Biol (1993) 64.61

Structure and mechanism of the lactose permease of Escherichia coli. Science (2003) 10.57

Statistical potential for assessment and prediction of protein structures. Protein Sci (2006) 8.98

Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli. Science (2003) 7.96

High-resolution distance mapping in rhodopsin reveals the pattern of helix movement due to activation. Proc Natl Acad Sci U S A (2008) 5.06

Translocations through natural membranes. Adv Enzymol Relat Areas Mol Biol (1967) 3.77

Lessons from lactose permease. Annu Rev Biophys Biomol Struct (2006) 3.69

Cys-scanning mutagenesis: a novel approach to structure function relationships in polytopic membrane proteins. FASEB J (1998) 3.41

Lactose transport coupled to proton movements in Escherichia coli. Biochem Biophys Res Commun (1970) 3.32

The major facilitator superfamily. J Mol Microbiol Biotechnol (1999) 3.17

Ins and outs of major facilitator superfamily antiporters. Annu Rev Microbiol (2008) 3.11

The kamikaze approach to membrane transport. Nat Rev Mol Cell Biol (2001) 3.09

Structure of the multidrug transporter EmrD from Escherichia coli. Science (2006) 2.93

Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY. EMBO J (2006) 2.92

Structural determination of wild-type lactose permease. Proc Natl Acad Sci U S A (2007) 2.83

Sugar binding induces an outward facing conformation of LacY. Proc Natl Acad Sci U S A (2007) 2.66

Stoicheiometry of lactose-H+ symport across the plasma membrane of Escherichia coli. Biochem J (1973) 2.57

Quantitative analysis of cryo-EM density map segmentation by watershed and scale-space filtering, and fitting of structures by alignment to regions. J Struct Biol (2010) 2.48

Structure of a fucose transporter in an outward-open conformation. Nature (2010) 2.28

Single-molecule FRET reveals sugar-induced conformational dynamics in LacY. Proc Natl Acad Sci U S A (2007) 2.24

Families of transmembrane sugar transport proteins. Mol Microbiol (2000) 2.23

Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 1. Effect of pH on efflux, exchange, and counterflow. Biochemistry (1979) 2.07

Crystal structure of a prokaryotic homologue of the mammalian oligopeptide-proton symporters, PepT1 and PepT2. EMBO J (2010) 2.05

Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 2. Effect of imposed delata psi, delta pH, and Delta mu H+. Biochemistry (1979) 1.85

The alternating-access mechanism of MFS transporters arises from inverted-topology repeats. J Mol Biol (2011) 1.83

A mutation in the lactose permease of Escherichia coli that decreases conformational flexibility and increases protein stability. Biochemistry (2003) 1.62

Lactose permease and the alternating access mechanism. Biochemistry (2011) 1.62

Fourier transform infrared spectroscopy reveals a rigid alpha-helical assembly for the tetrameric Streptomyces lividans K+ channel. Proc Natl Acad Sci U S A (1998) 1.62

cys154 Is important for lac permease activity in Escherichia coli. Biochem Biophys Res Commun (1985) 1.48

Interhelical packing modulates conformational flexibility in the lactose permease of Escherichia coli. Biochemistry (2005) 1.47

Energetics of ligand-induced conformational flexibility in the lactose permease of Escherichia coli. J Biol Chem (2006) 1.46

Purified reconstituted lac carrier protein from Escherichia coli is fully functional. Proc Natl Acad Sci U S A (1984) 1.40

Structural model for 12-helix transporters belonging to the major facilitator superfamily. J Bacteriol (2003) 1.38

The Cys154-->Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway. J Mol Biol (2008) 1.33

Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition. Proc Natl Acad Sci U S A (2011) 1.33

Exchange, efflux, and substrate binding by cysteine mutants of the lactose permease of Escherichia coli. Biochemistry (1993) 1.30

Arg-302 facilitates deprotonation of Glu-325 in the transport mechanism of the lactose permease from Escherichiacoli. Proc Natl Acad Sci U S A (2001) 1.28

Electrophysiological characterization of LacY. Proc Natl Acad Sci U S A (2009) 1.26

Fourier transform infrared analysis of purified lactose permease: a monodisperse lactose permease preparation is stably folded, alpha-helical, and highly accessible to deuterium exchange. Biochemistry (1998) 1.25

Residues in the H+ translocation site define the pKa for sugar binding to LacY. Biochemistry (2009) 1.17

Direct measurement of lactose/proton symport in Escherichia coli membrane vesicles: further evidence for the involvement of histidine residue(s). Biochemistry (1982) 1.15

Improved helix and kink characterization in membrane proteins allows evaluation of kink sequence predictors. J Chem Inf Model (2010) 1.08

Conformational analysis of a nitroxide side chain in an α-helix with density functional theory. J Phys Chem B (2010) 1.08

Helix packing in the lactose permease of Escherichia coli: localization of helix VI. J Mol Biol (2001) 1.01

Helix dynamics in LacY: helices II and IV. J Mol Biol (2010) 1.00

Opening the periplasmic cavity in lactose permease is the limiting step for sugar binding. Proc Natl Acad Sci U S A (2011) 1.00

Structural characterization of the osmosensor ProP. Biochim Biophys Acta (2009) 1.00

Evidence for an intermediate conformational state of LacY. Proc Natl Acad Sci U S A (2012) 0.97

An early event in the transport mechanism of LacY protein: interaction between helices V and I. J Biol Chem (2011) 0.89

Structure and mechanism of the lactose permease of Escherichia coli. Science (2003) 10.57

Lessons from lactose permease. Annu Rev Biophys Biomol Struct (2006) 3.69

Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY. EMBO J (2006) 2.92

Structural determination of wild-type lactose permease. Proc Natl Acad Sci U S A (2007) 2.83

Sugar binding induces an outward facing conformation of LacY. Proc Natl Acad Sci U S A (2007) 2.66

Single-molecule FRET reveals sugar-induced conformational dynamics in LacY. Proc Natl Acad Sci U S A (2007) 2.24

Manipulating phospholipids for crystallization of a membrane transport protein. Proc Natl Acad Sci U S A (2006) 1.71

Opening and closing of the periplasmic gate in lactose permease. Proc Natl Acad Sci U S A (2008) 1.68

A mutation in the lactose permease of Escherichia coli that decreases conformational flexibility and increases protein stability. Biochemistry (2003) 1.62

Lactose permease and the alternating access mechanism. Biochemistry (2011) 1.62

Binding affinity of lactose permease is not altered by the H+ electrochemical gradient. Proc Natl Acad Sci U S A (2004) 1.58

Aromatic stacking in the sugar binding site of the lactose permease. Biochemistry (2003) 1.49

Interhelical packing modulates conformational flexibility in the lactose permease of Escherichia coli. Biochemistry (2005) 1.47

Energetics of ligand-induced conformational flexibility in the lactose permease of Escherichia coli. J Biol Chem (2006) 1.46

Role of YidC in folding of polytopic membrane proteins. J Cell Biol (2004) 1.43

Lactose permease as a paradigm for membrane transport proteins (Review). Mol Membr Biol (2004) 1.43

The lactose permease of Escherichia coli: overall structure, the sugar-binding site and the alternating access model for transport. FEBS Lett (2003) 1.36

Exploiting luminescence spectroscopy to elucidate the interaction between sugar and a tryptophan residue in the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A (2003) 1.36

Structural conservation in the major facilitator superfamily as revealed by comparative modeling. Protein Sci (2004) 1.36

Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition. Proc Natl Acad Sci U S A (2011) 1.33

The Cys154-->Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway. J Mol Biol (2008) 1.33

Direct sugar binding to LacY measured by resonance energy transfer. Biochemistry (2006) 1.32

Probing of the rates of alternating access in LacY with Trp fluorescence. Proc Natl Acad Sci U S A (2009) 1.31

Binding of hydrophobic D-galactopyranosides to the lactose permease of Escherichia coli. Biochemistry (2002) 1.28

An approach to membrane protein structure without crystals. Proc Natl Acad Sci U S A (2002) 1.28

Site-directed alkylation of LacY: effect of the proton electrochemical gradient. J Mol Biol (2007) 1.28

Electrophysiological characterization of LacY. Proc Natl Acad Sci U S A (2009) 1.26

Evolutionary mix-and-match with MFS transporters. Proc Natl Acad Sci U S A (2013) 1.26

Protonation and sugar binding to LacY. Proc Natl Acad Sci U S A (2008) 1.25

Structure of sugar-bound LacY. Proc Natl Acad Sci U S A (2014) 1.23

Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking. Proc Natl Acad Sci U S A (2002) 1.22

Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily. Curr Opin Struct Biol (2004) 1.22

Residues in the H+ translocation site define the pKa for sugar binding to LacY. Biochemistry (2009) 1.17

Elucidation of substrate binding interactions in a membrane transport protein by mass spectrometry. EMBO J (2003) 1.16

Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins. Nat Protoc (2007) 1.15

Sugar recognition by the lactose permease of Escherichia coli. J Biol Chem (2004) 1.12

Intermolecular thiol cross-linking via loops in the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A (2003) 1.11

Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane. Proc Natl Acad Sci U S A (2010) 1.11

Sequence alignment and homology threading reveals prokaryotic and eukaryotic proteins similar to lactose permease. J Mol Biol (2006) 1.09

Delineating electrogenic reactions during lactose/H+ symport. Biochemistry (2010) 1.08

Residues gating the periplasmic pathway of LacY. J Mol Biol (2009) 1.05

In vitro synthesis of lactose permease to probe the mechanism of membrane insertion and folding. J Biol Chem (2003) 1.05

Evolutionary mix-and-match with MFS transporters II. Proc Natl Acad Sci U S A (2013) 1.05

Functional architecture of MFS D-glucose transporters. Proc Natl Acad Sci U S A (2014) 1.04

Conservation of residues involved in sugar/H(+) symport by the sucrose permease of Escherichia coli relative to lactose permease. J Mol Biol (2006) 1.04

Changing the lactose permease of Escherichia coli into a galactose-specific symporter. Proc Natl Acad Sci U S A (2002) 1.04

2007 annual progress report synopsis of the Center for Structures of Membrane Proteins. J Struct Funct Genomics (2009) 1.03

Site-directed alkylation of cysteine replacements in the lactose permease of Escherichia coli: helices I, III, VI, and XI. Biochemistry (2006) 1.02

The Sec-independent function of Escherichia coli YidC is evolutionary-conserved and essential. J Biol Chem (2005) 1.02

Clogging the periplasmic pathway in LacY. Biochemistry (2009) 1.01

Helix dynamics in LacY: helices II and IV. J Mol Biol (2010) 1.00

Opening the periplasmic cavity in lactose permease is the limiting step for sugar binding. Proc Natl Acad Sci U S A (2011) 1.00

Helices VII and X in the lactose permease of Escherichia coli: proximity and ligand-induced distance changes. J Mol Biol (2002) 0.98

Binding of enzyme IIAGlc, a component of the phosphoenolpyruvate:sugar phosphotransferase system, to the Escherichia coli lactose permease. Biochemistry (2002) 0.98

Monitoring conformational rearrangements in the substrate-binding site of a membrane transport protein by mass spectrometry. J Biol Chem (2004) 0.98

Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport. Biochemistry (2011) 0.98

SecY alterations that impair membrane protein folding and generate a membrane stress. J Cell Biol (2007) 0.97

Evidence for an intermediate conformational state of LacY. Proc Natl Acad Sci U S A (2012) 0.97

Probing the mechanism of a membrane transport protein with affinity inactivators. J Biol Chem (2002) 0.96

Functional overlap but lack of complete cross-complementation of Streptococcus mutans and Escherichia coli YidC orthologs. J Bacteriol (2008) 0.95

Role of protons in sugar binding to LacY. Proc Natl Acad Sci U S A (2012) 0.91

Dynamics of the L-fucose/H+ symporter revealed by fluorescence spectroscopy. Proc Natl Acad Sci U S A (2012) 0.91

Exploring the role of a unique carboxyl residue in EmrE by mass spectrometry. J Biol Chem (2004) 0.89

Proton-coupled dynamics in lactose permease. Structure (2012) 0.89

Site-directed sulfhydryl labeling of helix IX in the lactose permease of Escherichia coli. Biochemistry (2003) 0.89

Asymmetric binding of membrane proteins to GroEL. Arch Biochem Biophys (2005) 0.89

An early event in the transport mechanism of LacY protein: interaction between helices V and I. J Biol Chem (2011) 0.89

Manipulating conformational equilibria in the lactose permease of Escherichia coli. J Mol Biol (2002) 0.88

Trp replacements for tightly interacting Gly-Gly pairs in LacY stabilize an outward-facing conformation. Proc Natl Acad Sci U S A (2013) 0.86

Substrate-induced changes in the structural properties of LacY. Proc Natl Acad Sci U S A (2014) 0.86

Engineering a terbium-binding site into an integral membrane protein for luminescence energy transfer. Proc Natl Acad Sci U S A (2002) 0.85

Integrating mass spectrometry into membrane protein drug discovery. Curr Opin Drug Discov Devel (2004) 0.84

Properties of a LacY efflux mutant. Biochemistry (2009) 0.83

Sugar recognition by CscB and LacY. Biochemistry (2011) 0.82

Electrophysiological characterization of uncoupled mutants of LacY. Biochemistry (2013) 0.82

YidC protein, a molecular chaperone for LacY protein folding via the SecYEG protein machinery. J Biol Chem (2013) 0.81

Role of the irreplaceable residues in the LacY alternating access mechanism. Proc Natl Acad Sci U S A (2012) 0.81

Ligand-induced differences in secondary structure of the Vibrio parahaemolyticus Na+/galactose cotransporter. Biochemistry (2002) 0.80

Characterization of a lactose permease mutant that binds IIAGlc in the absence of ligand. Biochemistry (2003) 0.78

The periplasmic cavity of LacY mutant Cys154→Gly: how open is open? Biochemistry (2013) 0.78

Galactoside-binding site in LacY. Biochemistry (2014) 0.77

Role of Conserved Gly-Gly Pairs on the Periplasmic Side of LacY. Biochemistry (2016) 0.75