Published in Biochemistry on March 19, 2013
Structural basis of lipid binding for the membrane-embedded tetraacyldisaccharide-1-phosphate 4'-kinase LpxK. J Biol Chem (2014) 0.75
Structure, inhibition, and regulation of essential lipid A enzymes. Biochim Biophys Acta (2016) 0.75
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr D Biol Crystallogr (2010) 108.52
Features and development of Coot. Acta Crystallogr D Biol Crystallogr (2010) 89.46
MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr D Biol Crystallogr (2009) 53.36
Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J (1982) 38.14
The Pfam protein families database. Nucleic Acids Res (2011) 33.46
Lipopolysaccharide endotoxins. Annu Rev Biochem (2001) 18.88
Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J Mol Biol (1996) 13.17
High efficiency transformation of Escherichia coli with plasmids. Gene (1990) 10.65
The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex. Nature (2009) 8.99
Lipid A modification systems in gram-negative bacteria. Annu Rev Biochem (2007) 8.60
Kinetic and catalytic mechanisms of protein kinases. Chem Rev (2001) 3.80
Catalytic triads and their relatives. Trends Biochem Sci (1998) 3.06
Evolution and classification of P-loop kinases and related proteins. J Mol Biol (2003) 2.52
Lipid signaling enzymes and surface dilution kinetics. J Biol Chem (1995) 2.41
Structure and function of sulfotransferases. Arch Biochem Biophys (2001) 1.85
Sequence and structure classification of kinases. J Mol Biol (2002) 1.84
Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli. J Biol Chem (1992) 1.59
The intrinsic pKa-values of functional groups in enzymes: improper deductions from the pH-dependence of steady-state parameters. CRC Crit Rev Biochem (1976) 1.48
Identification of the gene encoding the Escherichia coli lipid A 4'-kinase. Facile phosphorylation of endotoxin analogs with recombinant LpxK. J Biol Chem (1997) 1.40
Escherichia coli diacylglycerol kinase is an evolutionarily optimized membrane enzyme and catalyzes direct phosphoryl transfer. J Biol Chem (1997) 1.26
Kinetic analysis of the zinc-dependent deacetylase in the lipid A biosynthetic pathway. Biochemistry (2005) 1.21
Steady-state kinetics and mechanism of LpxD, the N-acyltransferase of lipid A biosynthesis. Biochemistry (2008) 1.17
Purification and mutagenesis of LpxL, the lauroyltransferase of Escherichia coli lipid A biosynthesis. Biochemistry (2008) 1.16
The biosynthesis of gram-negative endotoxin. A novel kinase in Escherichia coli membranes that incorporates the 4'-phosphate of lipid A. J Biol Chem (1987) 1.13
Characterization of a novel lipid A containing D-galacturonic acid that replaces phosphate residues. The structure of the lipid a of the lipopolysaccharide from the hyperthermophilic bacterium Aquifex pyrophilus. J Biol Chem (2000) 1.06
Accumulation of a lipid A precursor lacking the 4'-phosphate following inactivation of the Escherichia coli lpxK gene. J Biol Chem (1998) 1.03
Biochemical and X-ray crystallographic studies on shikimate kinase: the important structural role of the P-loop lysine. Protein Sci (2001) 1.02
Evidence supporting catalytic roles for aspartate residues in phosphoribulokinase. Biochemistry (1994) 0.92
WaaA of the hyperthermophilic bacterium Aquifex aeolicus is a monofunctional 3-deoxy-D-manno-oct-2-ulosonic acid transferase involved in lipopolysaccharide biosynthesis. J Biol Chem (2009) 0.90
Phosphoryl transfer by a concerted reaction mechanism in UMP/CMP-kinase. Protein Sci (2000) 0.84
Crystal structure of LpxK, the 4'-kinase of lipid A biosynthesis and atypical P-loop kinase functioning at the membrane interface. Proc Natl Acad Sci U S A (2012) 0.83
The active site histidines of creatine kinase. A critical role of His 61 situated on a flexible loop. Protein Sci (1997) 0.80
Methyl 2-acetamido-2-deoxy-β-D-glucopyranoside dihydrate and methyl 2-formamido-2-deoxy-β-D-glucopyranoside. Acta Crystallogr C (2011) 0.78
A comprehensive classification system for lipids. J Lipid Res (2005) 6.52
LMSD: LIPID MAPS structure database. Nucleic Acids Res (2006) 6.21
Lipidomics reveals a remarkable diversity of lipids in human plasma. J Lipid Res (2010) 4.14
Update of the LIPID MAPS comprehensive classification system for lipids. J Lipid Res (2008) 4.01
Drosophila PIWI associates with chromatin and interacts directly with HP1a. Genes Dev (2007) 3.46
Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates. Science (2007) 2.58
SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder. Cell (2010) 2.49
A conserved family of enzymes that phosphorylate inositol hexakisphosphate. Science (2007) 2.43
Solution structure and dynamics of the outer membrane enzyme PagP by NMR. Proc Natl Acad Sci U S A (2002) 2.39
Regulated accumulation of desmosterol integrates macrophage lipid metabolism and inflammatory responses. Cell (2012) 2.38
MsbA-dependent translocation of lipids across the inner membrane of Escherichia coli. J Biol Chem (2004) 2.17
A mouse macrophage lipidome. J Biol Chem (2010) 2.14
LPS remodeling is an evolved survival strategy for bacteria. Proc Natl Acad Sci U S A (2012) 2.05
Modulation of human nuclear receptor LRH-1 activity by phospholipids and SHP. Nat Struct Mol Biol (2005) 2.03
A novel class of potent influenza virus inhibitors: polysubstituted acylthiourea and its fused heterocycle derivatives. Bioorg Med Chem Lett (2005) 2.01
Mitochondrial phosphatase PTPMT1 is essential for cardiolipin biosynthesis. Cell Metab (2011) 1.97
Structure of the ubiquitin-binding zinc finger domain of human DNA Y-polymerase eta. EMBO Rep (2007) 1.91
A slow, tight-binding inhibitor of the zinc-dependent deacetylase LpxC of lipid A biosynthesis with antibiotic activity comparable to ciprofloxacin. Biochemistry (2005) 1.86
Inositol diphosphate signaling regulates telomere length. J Biol Chem (2004) 1.85
ATPase activity of the MsbA lipid flippase of Escherichia coli. J Biol Chem (2002) 1.83
Functional anatomy of phospholipid binding and regulation of phosphoinositide homeostasis by proteins of the sec14 superfamily. Mol Cell (2008) 1.82
MsbA transporter-dependent lipid A 1-dephosphorylation on the periplasmic surface of the inner membrane: topography of francisella novicida LpxE expressed in Escherichia coli. J Biol Chem (2004) 1.73
Loss of outer membrane proteins without inhibition of lipid export in an Escherichia coli YaeT mutant. J Biol Chem (2005) 1.73
Generation of phytate-free seeds in Arabidopsis through disruption of inositol polyphosphate kinases. Proc Natl Acad Sci U S A (2005) 1.68
Expression cloning and periplasmic orientation of the Francisella novicida lipid A 4'-phosphatase LpxF. J Biol Chem (2006) 1.67
Characteristics of the interaction of a synthetic human tristetraprolin tandem zinc finger peptide with AU-rich element-containing RNA substrates. J Biol Chem (2003) 1.67
A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function oF UDP-4-deoxy-4-formamido-L-arabinose. J Biol Chem (2005) 1.66
Mechanism and inhibition of LpxC: an essential zinc-dependent deacetylase of bacterial lipid A synthesis. Curr Pharm Biotechnol (2008) 1.66
Attenuated virulence of a Francisella mutant lacking the lipid A 4'-phosphatase. Proc Natl Acad Sci U S A (2007) 1.64
A triple mutant of Escherichia coli lacking secondary acyl chains on lipid A. J Biol Chem (2002) 1.60
A small-molecule inhibitor of isoprenylcysteine carboxyl methyltransferase with antitumor activity in cancer cells. Proc Natl Acad Sci U S A (2005) 1.59
Oxidative decarboxylation of UDP-glucuronic acid in extracts of polymyxin-resistant Escherichia coli. Origin of lipid a species modified with 4-amino-4-deoxy-L-arabinose. J Biol Chem (2001) 1.59
Structure of the LpxC deacetylase with a bound substrate-analog inhibitor. Nat Struct Biol (2003) 1.57
Polar Fourier transforms of radially sampled NMR data. J Magn Reson (2006) 1.56
Origin of lipid A species modified with 4-amino-4-deoxy-L-arabinose in polymyxin-resistant mutants of Escherichia coli. An aminotransferase (ArnB) that generates UDP-4-deoxyl-L-arabinose. J Biol Chem (2003) 1.55
A new link between the c-Abl tyrosine kinase and phosphoinositide signalling through PLC-gamma1. Nat Cell Biol (2003) 1.53
The T4 phage SF1B helicase Dda is structurally optimized to perform DNA strand separation. Structure (2012) 1.52
Periplasmic cleavage and modification of the 1-phosphate group of Helicobacter pylori lipid A. J Biol Chem (2004) 1.52
High resolution 4-D spectroscopy with sparse concentric shell sampling and FFT-CLEAN. J Biomol NMR (2008) 1.49
Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1. Proc Natl Acad Sci U S A (2005) 1.49
An essential role for an inositol polyphosphate multikinase, Ipk2, in mouse embryogenesis and second messenger production. Proc Natl Acad Sci U S A (2005) 1.48
cis-Proline-mediated Ser(P)5 dephosphorylation by the RNA polymerase II C-terminal domain phosphatase Ssu72. J Biol Chem (2010) 1.47
Structure and biosynthesis of free lipid A molecules that replace lipopolysaccharide in Francisella tularensis subsp. novicida. Biochemistry (2006) 1.47
Subcellular organelle lipidomics in TLR-4-activated macrophages. J Lipid Res (2010) 1.46
A methylated phosphate group and four amide-linked acyl chains in leptospira interrogans lipid A. The membrane anchor of an unusual lipopolysaccharide that activates TLR2. J Biol Chem (2004) 1.44
Structural analysis and detection of biological inositol pyrophosphates reveal that the family of VIP/diphosphoinositol pentakisphosphate kinases are 1/3-kinases. J Biol Chem (2008) 1.44
Role of Mg2+ and pH in the modification of Salmonella lipid A after endocytosis by macrophage tumour cells. Mol Microbiol (2005) 1.41
Refined solution structure of the LpxC-TU-514 complex and pKa analysis of an active site histidine: insights into the mechanism and inhibitor design. Biochemistry (2005) 1.40
An outer membrane enzyme encoded by Salmonella typhimurium lpxR that removes the 3'-acyloxyacyl moiety of lipid A. J Biol Chem (2006) 1.39
AglJ adds the first sugar of the N-linked pentasaccharide decorating the Haloferax volcanii S-layer glycoprotein. J Bacteriol (2010) 1.38
PACES: Protein sequential assignment by computer-assisted exhaustive search. J Biomol NMR (2003) 1.37
Resistance to the antimicrobial peptide polymyxin requires myristoylation of Escherichia coli and Salmonella typhimurium lipid A. J Biol Chem (2005) 1.35
Structure and function of both domains of ArnA, a dual function decarboxylase and a formyltransferase, involved in 4-amino-4-deoxy-L-arabinose biosynthesis. J Biol Chem (2005) 1.34
Differential TLR recognition of leptospiral lipid A and lipopolysaccharide in murine and human cells. J Immunol (2005) 1.33
Inositol phosphate synthesis and the nuclear processes they affect. Curr Opin Cell Biol (2010) 1.33
Cloning and characterization of two human VIP1-like inositol hexakisphosphate and diphosphoinositol pentakisphosphate kinases. J Biol Chem (2007) 1.32
Molecular and biochemical characterization of two plant inositol polyphosphate 6-/3-/5-kinases. J Biol Chem (2002) 1.30
Effects of plant species coexistence on soil enzyme activities and soil microbial community structure under Cd and Pb combined pollution. J Environ Sci (China) (2010) 1.30
Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates. Proc Natl Acad Sci U S A (2012) 1.29
Crystal structure and acyl chain selectivity of Escherichia coli LpxD, the N-acyltransferase of lipid A biosynthesis. Biochemistry (2009) 1.28
Inhibition of lipid A biosynthesis as the primary mechanism of CHIR-090 antibiotic activity in Escherichia coli. Biochemistry (2007) 1.28
Structural basis for the acyl chain selectivity and mechanism of UDP-N-acetylglucosamine acyltransferase. Proc Natl Acad Sci U S A (2007) 1.26
Molecular validation of LpxC as an antibacterial drug target in Pseudomonas aeruginosa. Antimicrob Agents Chemother (2006) 1.25
Structure of the deacetylase LpxC bound to the antibiotic CHIR-090: Time-dependent inhibition and specificity in ligand binding. Proc Natl Acad Sci U S A (2007) 1.24
Potent, novel in vitro inhibitors of the Pseudomonas aeruginosa deacetylase LpxC. J Med Chem (2002) 1.24
(4,2)D Projection--reconstruction experiments for protein backbone assignment: application to human carbonic anhydrase II and calbindin D(28K). J Am Chem Soc (2005) 1.23
The ADAM10 prodomain is a specific inhibitor of ADAM10 proteolytic activity and inhibits cellular shedding events. J Biol Chem (2007) 1.22
Kinetic analysis of the zinc-dependent deacetylase in the lipid A biosynthetic pathway. Biochemistry (2005) 1.21
Radial sampling for fast NMR: Concepts and practices over three decades. Prog Nucl Magn Reson Spectrosc (2010) 1.21
The low affinity IgE receptor (CD23) is cleaved by the metalloproteinase ADAM10. J Biol Chem (2007) 1.21
Cross-linking electrospun type II collagen tissue engineering scaffolds with carbodiimide in ethanol. Tissue Eng (2007) 1.20
Sampling of the NMR time domain along concentric rings. J Magn Reson (2006) 1.20
Expression cloning and biochemical characterization of a Rhizobium leguminosarum lipid A 1-phosphatase. J Biol Chem (2003) 1.19
An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli. J Biol Chem (2007) 1.18
Fatty acid synthase inhibitors: new directions for oncology. Expert Opin Investig Drugs (2007) 1.18
Characterization of a selective inhibitor of inositol hexakisphosphate kinases: use in defining biological roles and metabolic relationships of inositol pyrophosphates. J Biol Chem (2009) 1.17
Characterization of hepatic tumors: value of contrast-enhanced coded phase-inversion harmonic angio. AJR Am J Roentgenol (2004) 1.17
An Escherichia coli mutant lacking the cold shock-induced palmitoleoyltransferase of lipid A biosynthesis: absence of unsaturated acyl chains and antibiotic hypersensitivity at 12 degrees C. J Biol Chem (2002) 1.17
Identification of N-acylphosphatidylserine molecules in eukaryotic cells. Biochemistry (2007) 1.17
Steady-state kinetics and mechanism of LpxD, the N-acyltransferase of lipid A biosynthesis. Biochemistry (2008) 1.17
The lipid lysyl-phosphatidylglycerol is present in membranes of Rhizobium tropici CIAT899 and confers increased resistance to polymyxin B under acidic growth conditions. Mol Plant Microbe Interact (2007) 1.16
Purification and mutagenesis of LpxL, the lauroyltransferase of Escherichia coli lipid A biosynthesis. Biochemistry (2008) 1.16
Groundwater nitrogen pollution and assessment of its health risks: a case study of a typical village in rural-urban continuum, China. PLoS One (2012) 1.15
Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli. Anal Biochem (2005) 1.15
Salmonella synthesizing 1-dephosphorylated [corrected] lipopolysaccharide exhibits low endotoxic activity while retaining its immunogenicity. J Immunol (2011) 1.15
Structure of UDP-N-acetylglucosamine acyltransferase with a bound antibacterial pentadecapeptide. Proc Natl Acad Sci U S A (2006) 1.15
Identification of self-lipids presented by CD1c and CD1d proteins. J Biol Chem (2011) 1.14
Species-specific and inhibitor-dependent conformations of LpxC: implications for antibiotic design. Chem Biol (2010) 1.14
Roles of inositol phosphates and inositol pyrophosphates in development, cell signaling and nuclear processes. Adv Enzyme Regul (2009) 1.13
Filtered backprojection for the reconstruction of a high-resolution (4,2)D CH3-NH NOESY spectrum on a 29 kDa protein. J Am Chem Soc (2005) 1.13
Sinorhizobium meliloti phospholipase C required for lipid remodeling during phosphorus limitation. Proc Natl Acad Sci U S A (2009) 1.11
Rapid protein global fold determination using ultrasparse sampling, high-dynamic range artifact suppression, and time-shared NOESY. J Am Chem Soc (2012) 1.11
SnapShot: Inositol phosphates. Cell (2010) 1.10