Published in Chem Rev on March 21, 2014
Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET. Nat Chem (2014) 1.82
Site-specific antibody-drug conjugates: the nexus of bioorthogonal chemistry, protein engineering, and drug development. Bioconjug Chem (2015) 1.58
Genetic code expansion in stable cell lines enables encoded chromatin modification. Nat Methods (2016) 1.50
A chemical biology route to site-specific authentic protein modifications. Science (2016) 1.49
Click chemistry in complex mixtures: bioorthogonal bioconjugation. Chem Biol (2014) 1.40
Selective, rapid and optically switchable regulation of protein function in live mammalian cells. Nat Chem (2015) 1.08
π-Clamp-mediated cysteine conjugation. Nat Chem (2015) 1.06
Efficient multisite unnatural amino acid incorporation in mammalian cells via optimized pyrrolysyl tRNA synthetase/tRNA expression and engineered eRF1. J Am Chem Soc (2014) 1.05
Histones: at the crossroads of peptide and protein chemistry. Chem Rev (2014) 1.01
CalFluors: A Universal Motif for Fluorogenic Azide Probes across the Visible Spectrum. J Am Chem Soc (2015) 0.98
Concerted, rapid, quantitative, and site-specific dual labeling of proteins. J Am Chem Soc (2014) 0.97
Site-selective protein-modification chemistry for basic biology and drug development. Nat Chem (2015) 0.96
Genetic code expansion enables live-cell and super-resolution imaging of site-specifically labeled cellular proteins. J Am Chem Soc (2015) 0.96
Design strategies for bioorthogonal smart probes. Org Biomol Chem (2014) 0.95
Diazo groups endure metabolism and enable chemoselectivity in cellulo. J Am Chem Soc (2015) 0.88
Directed evolution of the tryptophan synthase β-subunit for stand-alone function recapitulates allosteric activation. Proc Natl Acad Sci U S A (2015) 0.88
Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion. J Biol Chem (2015) 0.87
In vivo covalent cross-linking of photon-converted rare-earth nanostructures for tumour localization and theranostics. Nat Commun (2016) 0.87
New organocatalyst scaffolds with high activity in promoting hydrazone and oxime formation at neutral pH. Org Lett (2014) 0.83
How to Measure Molecular Forces in Cells: A Guide to Evaluating Genetically-Encoded FRET-Based Tension Sensors. Cell Mol Bioeng (2014) 0.82
A noncanonical function of sortase enables site-specific conjugation of small molecules to lysine residues in proteins. Angew Chem Int Ed Engl (2014) 0.81
Systematic Evaluation of Bioorthogonal Reactions in Live Cells with Clickable HaloTag Ligands: Implications for Intracellular Imaging. J Am Chem Soc (2015) 0.81
Gleaning unexpected fruits from hard-won synthetases: probing principles of permissivity in non-canonical amino acid-tRNA synthetases. Chembiochem (2014) 0.81
Genetic code expansion in the mouse brain. Nat Chem Biol (2016) 0.80
Specific and quantitative labeling of biomolecules using click chemistry. Front Physiol (2014) 0.80
Site-Specific Bioorthogonal Labeling for Fluorescence Imaging of Intracellular Proteins in Living Cells. J Am Chem Soc (2016) 0.80
Monitoring Astrocytic Proteome Dynamics by Cell Type-Specific Protein Labeling. PLoS One (2015) 0.80
Site-Specifically Labeled Immunoconjugates for Molecular Imaging-Part 2: Peptide Tags and Unnatural Amino Acids. Mol Imaging Biol (2016) 0.79
Rapid Bioorthogonal Chemistry Turn-on through Enzymatic or Long Wavelength Photocatalytic Activation of Tetrazine Ligation. J Am Chem Soc (2016) 0.79
Site-Specific Glycoconjugation of Protein via Bioorthogonal Tetrazine Cycloaddition with a Genetically Encoded trans-Cyclooctene or Bicyclononyne. Bioconjug Chem (2015) 0.79
Using non-enzymatic chemistry to influence microbial metabolism. Curr Opin Chem Biol (2015) 0.79
Progress and prospects for small-molecule probes of bacterial imaging. Nat Chem Biol (2016) 0.79
Fluorogenic Strain-Promoted Alkyne-Diazo Cycloadditions. Chemistry (2015) 0.78
Micelle-Enhanced Bioorthogonal Labeling of Genetically Encoded Azido Groups on the Lipid-Embedded Surface of a GPCR. Chembiochem (2015) 0.78
Click Chemistry and Radiochemistry: The First Ten Years. Bioconjug Chem (2016) 0.78
Multiply labeling proteins for studies of folding and stability. Curr Opin Chem Biol (2015) 0.77
Tagging and Enriching Proteins Enables Cell-Specific Proteomics. Cell Chem Biol (2016) 0.77
Differentially Isotope-Labeled Nucleosomes To Study Asymmetric Histone Modification Crosstalk by Time-Resolved NMR Spectroscopy. Angew Chem Int Ed Engl (2016) 0.76
Site-specific labeling of proteins for electron microscopy. J Struct Biol (2015) 0.76
Aromatic thiol-mediated cleavage of N-O bonds enables chemical ubiquitylation of folded proteins. Nat Commun (2016) 0.75
Spatial and Temporal Resolution of Global Protein Synthesis during HSV Infection Using Bioorthogonal Precursors and Click Chemistry. PLoS Pathog (2016) 0.75
Strain-promoted reaction of 1,2,4-triazines with bicyclononynes. Chemistry (2015) 0.75
Side-chain-to-tail cyclization of ribosomally derived peptides promoted by aryl and alkyl amino-functionalized unnatural amino acids. Org Biomol Chem (2016) 0.75
Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins. Biotechnol Bioeng (2016) 0.75
Broad substrate tolerance of tubulin tyrosine ligase enables one-step site-specific enzymatic protein labeling. Chem Sci (2017) 0.75
Recent advances in covalent, site-specific protein immobilization. F1000Res (2016) 0.75
A Minimal, Unstrained S-Allyl Handle for Pre-Targeting Diels-Alder Bioorthogonal Labeling in Live Cells. Angew Chem Int Ed Engl (2016) 0.75
Tetrazine-Containing Amino Acid for Peptide Modification and Live Cell Labeling. PLoS One (2015) 0.75
Recent advances in synthetic biosafety. F1000Res (2016) 0.75
A little less conjugation, a little more accuracy. Nat Chem (2016) 0.75
Virus outbreaks in chemical and biological sensors. Sensors (Basel) (2014) 0.75
Conformational transition of FGFR kinase activation revealed by site-specific unnatural amino acid reporter and single molecule FRET. Sci Rep (2017) 0.75
Genetically Directed Production of Recombinant, Isosteric and Nonhydrolysable Ubiquitin Conjugates. Chembiochem (2016) 0.75
Spatiotemporal imaging of small GTPases activity in live cells. Proc Natl Acad Sci U S A (2016) 0.75
Modification of orthogonal tRNAs: unexpected consequences for sense codon reassignment. Nucleic Acids Res (2016) 0.75
Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle. Chem Sci (2016) 0.75
Probing the active site tryptophan of Staphylococcus aureus thioredoxin with an analog. Nucleic Acids Res (2015) 0.75
All about that Amide Bond: The Sixth Chemical Protein Synthesis (CPS) Meeting. Chembiochem (2015) 0.75
Chemical Methods for Encoding and Decoding of Posttranslational Modifications. Cell Chem Biol (2016) 0.75
Bioorthogonal Fluorescent Labelling of Biopolymers via Inverse Electron Demand Diels-Alder Reactions. Chembiochem (2017) 0.75
Copper-Catalyzed Click Reaction on/in Live Cells. Chem Sci (2016) 0.75
In situ click chemistry generation of cyclooxygenase-2 inhibitors. Nat Commun (2017) 0.75
In Vivo Evaluation of Site-Specifically PEGylated Chemically Self-Assembled Protein Nanostructures. Mol Pharm (2016) 0.75
Protein modification via alkyne hydrosilylation using a substoichiometric amount of ruthenium(ii) catalyst. Chem Sci (2017) 0.75
Click and Fluoresce: A Bioorthogonally Activated Smart Probe for Wash-free Fluorescent Labeling of Biomolecules. J Org Chem (2017) 0.75
Functional modulation and directed assembly of an enzyme through designed non-natural post-translation modification. Chem Sci (2015) 0.75
Direct fluorescent-dye labeling of α-tubulin in mammalian cells for live cell and super resolution imaging. Mol Biol Cell (2017) 0.75
Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome. Nature (2010) 3.69
Genetically encoding N(epsilon)-acetyllysine in recombinant proteins. Nat Chem Biol (2008) 3.68
A method for genetically installing site-specific acetylation in recombinant histones defines the effects of H3 K56 acetylation. Mol Cell (2009) 3.11
Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion. Nat Biotechnol (2007) 2.51
Light-activated kinases enable temporal dissection of signaling networks in living cells. J Am Chem Soc (2011) 2.22
A network of orthogonal ribosome x mRNA pairs. Nat Chem Biol (2005) 2.20
Substrate recognition by the AAA+ chaperone ClpB. Nat Struct Mol Biol (2004) 2.19
Expanding the genetic code of an animal. J Am Chem Soc (2011) 2.09
Engineered diubiquitin synthesis reveals Lys29-isopeptide specificity of an OTU deubiquitinase. Nat Chem Biol (2010) 2.09
Genetic encoding and labeling of aliphatic azides and alkynes in recombinant proteins via a pyrrolysyl-tRNA Synthetase/tRNA(CUA) pair and click chemistry. J Am Chem Soc (2009) 2.06
Genetic Encoding of bicyclononynes and trans-cyclooctenes for site-specific protein labeling in vitro and in live mammalian cells via rapid fluorogenic Diels-Alder reactions. J Am Chem Soc (2012) 2.03
Genetically encoded photocontrol of protein localization in mammalian cells. J Am Chem Soc (2010) 1.94
Adding amino acids with novel reactivity to the genetic code of Saccharomyces cerevisiae. J Am Chem Soc (2003) 1.93
Designer proteins: applications of genetic code expansion in cell biology. Nat Rev Mol Cell Biol (2012) 1.72
A genetically encoded fluorescent amino acid. Proc Natl Acad Sci U S A (2006) 1.66
DAXX envelops a histone H3.3-H4 dimer for H3.3-specific recognition. Nature (2012) 1.65
CPSF6 defines a conserved capsid interface that modulates HIV-1 replication. PLoS Pathog (2012) 1.64
Genetically encoding N(epsilon)-methyl-L-lysine in recombinant histones. J Am Chem Soc (2009) 1.60
Functional epitopes at the ribosome subunit interface. Nat Chem Biol (2006) 1.59
An ankyrin-repeat ubiquitin-binding domain determines TRABID's specificity for atypical ubiquitin chains. Nat Struct Mol Biol (2011) 1.55
Bioorthogonal reactions for labeling proteins. ACS Chem Biol (2014) 1.54
Expanding the genetic code of yeast for incorporation of diverse unnatural amino acids via a pyrrolysyl-tRNA synthetase/tRNA pair. J Am Chem Soc (2010) 1.53
Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains. Proc Natl Acad Sci U S A (2011) 1.52
Photo-cross-linking interacting proteins with a genetically encoded benzophenone. Nat Methods (2005) 1.49
Synthesis of orthogonal transcription-translation networks. Proc Natl Acad Sci U S A (2009) 1.46
Genetically encoded 1,2-aminothiols facilitate rapid and site-specific protein labeling via a bio-orthogonal cyanobenzothiazole condensation. J Am Chem Soc (2011) 1.42
Expanding the genetic code of Drosophila melanogaster. Nat Chem Biol (2012) 1.41
Traceless and site-specific ubiquitination of recombinant proteins. J Am Chem Soc (2011) 1.29
De novo generation of mutually orthogonal aminoacyl-tRNA synthetase/tRNA pairs. J Am Chem Soc (2010) 1.28
A view to a kill: ligands for Bcl-2 family proteins. Curr Opin Chem Biol (2002) 1.18
Acetylation of lysine 120 of p53 endows DNA-binding specificity at effective physiological salt concentration. Proc Natl Acad Sci U S A (2011) 1.16
Cellular logic with orthogonal ribosomes. J Am Chem Soc (2005) 1.12
Genetically encoding protein oxidative damage. J Am Chem Soc (2008) 1.08
Efficient multisite unnatural amino acid incorporation in mammalian cells via optimized pyrrolysyl tRNA synthetase/tRNA expression and engineered eRF1. J Am Chem Soc (2014) 1.05
Genetically directing ɛ-N, N-dimethyl-L-lysine in recombinant histones. Chem Biol (2010) 1.05
Photocontrol of tyrosine phosphorylation in mammalian cells via genetic encoding of photocaged tyrosine. J Am Chem Soc (2012) 1.02
Acetylation regulates cyclophilin A catalysis, immunosuppression and HIV isomerization. Nat Chem Biol (2010) 1.02
A dual role of H4K16 acetylation in the establishment of yeast silent chromatin. EMBO J (2011) 1.00
Genetically encoded light-activated transcription for spatiotemporal control of gene expression and gene silencing in mammalian cells. J Am Chem Soc (2013) 0.98
Concerted, rapid, quantitative, and site-specific dual labeling of proteins. J Am Chem Soc (2014) 0.97
Genetic encoding of photocaged cysteine allows photoactivation of TEV protease in live mammalian cells. J Am Chem Soc (2014) 0.96
Reprogramming the genetic code: from triplet to quadruplet codes. Angew Chem Int Ed Engl (2012) 0.93
Engineering Escherichia coli heat-resistance by synthetic gene amplification. Protein Eng Des Sel (2008) 0.92
Efficient and rapid C. elegans transgenesis by bombardment and hygromycin B selection. PLoS One (2013) 0.91
Genetically encoded optochemical probes for simultaneous fluorescence reporting and light activation of protein function with two-photon excitation. J Am Chem Soc (2014) 0.91
Evolved orthogonal ribosome purification for in vitro characterization. Nucleic Acids Res (2010) 0.84
Expanding nucleic acid function in vitro and in vivo. Curr Opin Chem Biol (2006) 0.82
Reprogramming the amino-acid substrate specificity of orthogonal aminoacyl-tRNA synthetases to expand the genetic code of eukaryotic cells. Nat Protoc (2007) 0.79
Orthogonal gene expression in Escherichia coli. Methods Enzymol (2011) 0.77
Genetic encoding of unnatural amino acids for labeling proteins. Methods Mol Biol (2015) 0.77
Fluorescent imaging: Shining a light into live cells. Nat Chem (2013) 0.75
A different life? Curr Opin Chem Biol (2012) 0.75