A view to a kill: ligands for Bcl-2 family proteins.

The BH3 mimetic ABT-737 targets selective Bcl-2 proteins and efficiently induces apoptosis via Bak/Bax if Mcl-1 is neutralized. Cancer Cell (2006) 10.19

Bim is a suppressor of Myc-induced mouse B cell leukemia. Proc Natl Acad Sci U S A (2004) 5.55

Enhanced metabolic stability and protein-binding properties of artificial alpha helices derived from a hydrogen-bond surrogate: application to Bcl-xL. Angew Chem Int Ed Engl (2005) 1.59

BH3 profiling--measuring integrated function of the mitochondrial apoptotic pathway to predict cell fate decisions. Cancer Lett (2012) 1.27

Endogenous Noxa Determines the Strong Proapoptotic Synergism of the BH3-Mimetic ABT-737 with Chemotherapeutic Agents in Human Melanoma Cells. Transl Oncol (2009) 1.09

To use MIBI or not to use MIBI? That is the question when assessing tumour cells. Eur J Nucl Med Mol Imaging (2005) 1.06

Molecular recognition of protein surfaces: high affinity ligands for the CBP KIX domain. J Am Chem Soc (2003) 1.01

NMR structure of a complex between MDM2 and a small molecule inhibitor. J Biomol NMR (2004) 0.91

Spirotetronate polyketides as leads in drug discovery. J Nat Prod (2014) 0.85

Inhibition of early 99mTc-MIBI uptake by Bcl-2 anti-apoptotic protein overexpression in untreated breast carcinoma. Eur J Nucl Med Mol Imaging (2003) 0.79

Miniature protein ligands for EVH1 domains: interplay between affinity, specificity, and cell motility. Biochemistry (2007) 0.78

Bcl-2 overexpression prevents 99mTc-MIBI uptake in breast cancer cell lines. Eur J Nucl Med Mol Imaging (2003) 0.78

Helix stability of oligoglycine, oligoalanine, and oligo-β-alanine dodecamers reflected by hydrogen-bond persistence. Proteins (2014) 0.76

Targeting ERK1/2-bim signaling cascades by BH3-mimetic ABT-737 as an alternative therapeutic strategy for oral cancer. Oncotarget (2015) 0.75

Effect of trifluoperazine on Tc-99m sestamibi uptake in patients with advanced nonsmall cell lung cancer. Indian J Nucl Med (2016) 0.75

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome. Nature (2010) 3.69

Genetically encoding N(epsilon)-acetyllysine in recombinant proteins. Nat Chem Biol (2008) 3.68

A method for genetically installing site-specific acetylation in recombinant histones defines the effects of H3 K56 acetylation. Mol Cell (2009) 3.11

Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion. Nat Biotechnol (2007) 2.51

Light-activated kinases enable temporal dissection of signaling networks in living cells. J Am Chem Soc (2011) 2.22

A network of orthogonal ribosome x mRNA pairs. Nat Chem Biol (2005) 2.20

Substrate recognition by the AAA+ chaperone ClpB. Nat Struct Mol Biol (2004) 2.19

Helical beta-peptide inhibitors of the p53-hDM2 interaction. J Am Chem Soc (2004) 2.15

Expanding the genetic code of an animal. J Am Chem Soc (2011) 2.09

Engineered diubiquitin synthesis reveals Lys29-isopeptide specificity of an OTU deubiquitinase. Nat Chem Biol (2010) 2.09

Genetic encoding and labeling of aliphatic azides and alkynes in recombinant proteins via a pyrrolysyl-tRNA Synthetase/tRNA(CUA) pair and click chemistry. J Am Chem Soc (2009) 2.06

Genetic Encoding of bicyclononynes and trans-cyclooctenes for site-specific protein labeling in vitro and in live mammalian cells via rapid fluorogenic Diels-Alder reactions. J Am Chem Soc (2012) 2.03

Genetically encoded photocontrol of protein localization in mammalian cells. J Am Chem Soc (2010) 1.94

Adding amino acids with novel reactivity to the genetic code of Saccharomyces cerevisiae. J Am Chem Soc (2003) 1.93

Cellular incorporation of unnatural amino acids and bioorthogonal labeling of proteins. Chem Rev (2014) 1.91

Surveying polypeptide and protein domain conformation and association with FlAsH and ReAsH. Nat Chem Biol (2007) 1.80

High-resolution structure of a beta-peptide bundle. J Am Chem Soc (2007) 1.74

Designer proteins: applications of genetic code expansion in cell biology. Nat Rev Mol Cell Biol (2012) 1.72

A genetically encoded fluorescent amino acid. Proc Natl Acad Sci U S A (2006) 1.66

DAXX envelops a histone H3.3-H4 dimer for H3.3-specific recognition. Nature (2012) 1.65

CPSF6 defines a conserved capsid interface that modulates HIV-1 replication. PLoS Pathog (2012) 1.64

Genetically encoding N(epsilon)-methyl-L-lysine in recombinant histones. J Am Chem Soc (2009) 1.60

Functional epitopes at the ribosome subunit interface. Nat Chem Biol (2006) 1.59

Inhibiting HIV fusion with a beta-peptide foldamer. J Am Chem Soc (2005) 1.55

An ankyrin-repeat ubiquitin-binding domain determines TRABID's specificity for atypical ubiquitin chains. Nat Struct Mol Biol (2011) 1.55

Bioorthogonal reactions for labeling proteins. ACS Chem Biol (2014) 1.54

Expanding the genetic code of yeast for incorporation of diverse unnatural amino acids via a pyrrolysyl-tRNA synthetase/tRNA pair. J Am Chem Soc (2010) 1.53

Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains. Proc Natl Acad Sci U S A (2011) 1.52

Photo-cross-linking interacting proteins with a genetically encoded benzophenone. Nat Methods (2005) 1.49

Synthesis of orthogonal transcription-translation networks. Proc Natl Acad Sci U S A (2009) 1.46

Selective recognition of protein tetraserine motifs with a cell-permeable, pro-fluorescent bis-boronic acid. J Am Chem Soc (2009) 1.44

Genetically encoded 1,2-aminothiols facilitate rapid and site-specific protein labeling via a bio-orthogonal cyanobenzothiazole condensation. J Am Chem Soc (2011) 1.42

Expanding the genetic code of Drosophila melanogaster. Nat Chem Biol (2012) 1.41

Toward beta-amino acid proteins: a cooperatively folded beta-peptide quaternary structure. J Am Chem Soc (2006) 1.36

Relationship between side chain structure and 14-helix stability of beta3-peptides in water. J Am Chem Soc (2005) 1.36

Traceless and site-specific ubiquitination of recombinant proteins. J Am Chem Soc (2011) 1.29

De novo generation of mutually orthogonal aminoacyl-tRNA synthetase/tRNA pairs. J Am Chem Soc (2010) 1.28

Intrinsically cell-permeable miniature proteins based on a minimal cationic PPII motif. J Am Chem Soc (2007) 1.27

Helix macrodipole control of beta 3 peptide 14-helix stability in water. J Am Chem Soc (2003) 1.27

A rapid library screen for tailoring beta-peptide structure and function. J Am Chem Soc (2005) 1.24

beta-Peptides as inhibitors of protein-protein interactions. Bioorg Med Chem (2005) 1.24

Minimally cationic cell-permeable miniature proteins via alpha-helical arginine display. J Am Chem Soc (2008) 1.23

Arginine topology controls escape of minimally cationic proteins from early endosomes to the cytoplasm. Chem Biol (2012) 1.18

Solution structure of a beta-peptide ligand for hDM2. J Am Chem Soc (2005) 1.18

Bipartite tetracysteine display reveals allosteric control of ligand-specific EGFR activation. ACS Chem Biol (2012) 1.18

Acetylation of lysine 120 of p53 endows DNA-binding specificity at effective physiological salt concentration. Proc Natl Acad Sci U S A (2011) 1.16

Bridged beta(3)-peptide inhibitors of p53-hDM2 complexation: correlation between affinity and cell permeability. J Am Chem Soc (2010) 1.14

Toward beta-amino acid proteins: design, synthesis, and characterization of a fifteen kilodalton beta-peptide tetramer. J Am Chem Soc (2008) 1.13

Cellular logic with orthogonal ribosomes. J Am Chem Soc (2005) 1.12

Sophistication of foldamer form and function in vitro and in vivo. Curr Opin Chem Biol (2007) 1.11

Biophysical and structural characterization of a robust octameric beta-peptide bundle. J Am Chem Soc (2007) 1.11

Miniature protein inhibitors of the p53-hDM2 interaction. Chembiochem (2006) 1.11

Design and Evolution of a Miniature Bcl-2 Binding Protein We thank the HHMI Biopolymer/Keck Foundation Biotechnology Resource Laboratory (Yale University School of Medicine, New Haven, CT) for oligonucleotide and peptide synthesis and amino acid analysis and Professor Jennifer Doudna (Yale University) for use of a Perseptive Voyager-DE (MALDI-TOF) mass spectrometer. We are grateful also to Dr. Junying Yuan and Dr. Alexi Degterev (Harvard Medical School) for a generous gift of Bcl-X(L)-His(6) and Stacey E. Rutledge for helpful comments. This work was supported by the National Institutes of Health. Angew Chem Int Ed Engl (2001) 1.11

Paralog-selective ligands for bcl-2 proteins. J Am Chem Soc (2005) 1.10

Biophysical characterization of a beta-peptide bundle: comparison to natural proteins. J Am Chem Soc (2007) 1.10

Genetically encoding protein oxidative damage. J Am Chem Soc (2008) 1.08

Beta-peptides with improved affinity for hDM2 and hDMX. Bioorg Med Chem (2009) 1.06

Bipartite tetracysteine display requires site flexibility for ReAsH coordination. Chembiochem (2009) 1.05

Efficient multisite unnatural amino acid incorporation in mammalian cells via optimized pyrrolysyl tRNA synthetase/tRNA expression and engineered eRF1. J Am Chem Soc (2014) 1.05

Genetically directing ɛ-N, N-dimethyl-L-lysine in recombinant histones. Chem Biol (2010) 1.05

Surveying protein structure and function using bis-arsenical small molecules. Acc Chem Res (2011) 1.04

Photocontrol of tyrosine phosphorylation in mammalian cells via genetic encoding of photocaged tyrosine. J Am Chem Soc (2012) 1.02

Acetylation regulates cyclophilin A catalysis, immunosuppression and HIV isomerization. Nat Chem Biol (2010) 1.02

Molecular recognition of protein surfaces: high affinity ligands for the CBP KIX domain. J Am Chem Soc (2003) 1.01

A dual role of H4K16 acetylation in the establishment of yeast silent chromatin. EMBO J (2011) 1.00

Super-resolution imaging of the Golgi in live cells with a bioorthogonal ceramide probe. Angew Chem Int Ed Engl (2014) 0.98

Genetically encoded light-activated transcription for spatiotemporal control of gene expression and gene silencing in mammalian cells. J Am Chem Soc (2013) 0.98

Miniature homeodomains: high specificity without an N-terminal arm. J Am Chem Soc (2003) 0.97

Concerted, rapid, quantitative, and site-specific dual labeling of proteins. J Am Chem Soc (2014) 0.97

Genetic encoding of photocaged cysteine allows photoactivation of TEV protease in live mammalian cells. J Am Chem Soc (2014) 0.96

Increasing the kinase specificity of k252a by protein surface recognition. Org Lett (2005) 0.96

In Silico Improvement of beta3-peptide inhibitors of p53 x hDM2 and p53 x hDMX. J Am Chem Soc (2009) 0.96

Visualizing protein partnerships in living cells and organisms. Curr Opin Chem Biol (2011) 0.95

Cell-permeable beta-peptide inhibitors of p53/hDM2 complexation. Chembiochem (2009) 0.95

Reprogramming the genetic code: from triplet to quadruplet codes. Angew Chem Int Ed Engl (2012) 0.93

A β-peptide agonist of the GLP-1 receptor, a class B GPCR. Org Lett (2013) 0.93

Engineering Escherichia coli heat-resistance by synthetic gene amplification. Protein Eng Des Sel (2008) 0.92

Relationship between salt-bridge identity and 14-helix stability of beta3-peptides in aqueous buffer. Org Lett (2006) 0.92

Tetrameric beta(3)-peptide bundles. Chembiochem (2008) 0.92

Efficient and rapid C. elegans transgenesis by bombardment and hygromycin B selection. PLoS One (2013) 0.91

Binding mode and transcriptional activation potential of high affinity ligands for the CBP KIX domain. J Am Chem Soc (2005) 0.91

Genetically encoded optochemical probes for simultaneous fluorescence reporting and light activation of protein function with two-photon excitation. J Am Chem Soc (2014) 0.91

Improved assays for determining the cytosolic access of peptides, proteins, and their mimetics. Biochemistry (2013) 0.90

Design and high-resolution structure of a β³-peptide bundle catalyst. J Am Chem Soc (2014) 0.90

Beta-peptide bundles with fluorous cores. J Am Chem Soc (2010) 0.89

High affinity, paralog-specific recognition of the Mena EVH1 domain by a miniature protein. J Am Chem Soc (2004) 0.89

Identification of a beta3-peptide HIV fusion inhibitor with improved potency in live cells. Bioorg Med Chem Lett (2009) 0.87

Inhibiting epidermal growth factor receptor at a distance. J Am Chem Soc (2014) 0.84

Lanthanide-mediated phosphoester hydrolysis and phosphate elimination from phosphopeptides. Chem Commun (Camb) (2005) 0.84

Evolved orthogonal ribosome purification for in vitro characterization. Nucleic Acids Res (2010) 0.84

Encodable activators of SRC family kinases. J Am Chem Soc (2006) 0.83

Enhancing β3 -peptide bundle stability by design. Chembiochem (2011) 0.82

Expanding nucleic acid function in vitro and in vivo. Curr Opin Chem Biol (2006) 0.82

Engineering a monomeric miniature protein. J Am Chem Soc (2007) 0.81

Visualizing tyrosine kinase activity with bipartite tetracysteine display. Chembiochem (2010) 0.80

Reprogramming the amino-acid substrate specificity of orthogonal aminoacyl-tRNA synthetases to expand the genetic code of eukaryotic cells. Nat Protoc (2007) 0.79

Direct visualization of protein association in living cells with complex-edited electron microscopy. Angew Chem Int Ed Engl (2010) 0.79

Interactions of AsCy3 with cysteine-rich peptides. Org Lett (2014) 0.79

Positive allostery in metal ion binding by a cooperatively folded β-peptide bundle. J Am Chem Soc (2014) 0.78

Miniature protein ligands for EVH1 domains: interplay between affinity, specificity, and cell motility. Biochemistry (2007) 0.78