Published in J Am Chem Soc on August 27, 2013
Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool. Biochim Biophys Acta (2014) 1.13
Optical Control of CRISPR/Cas9 Gene Editing. J Am Chem Soc (2015) 0.98
Genetically encoded optochemical probes for simultaneous fluorescence reporting and light activation of protein function with two-photon excitation. J Am Chem Soc (2014) 0.91
A Small-Molecule Photoactivatable Optical Sensor of Transmembrane Potential. J Am Chem Soc (2015) 0.86
Control of protein function through optochemical translocation. ACS Synth Biol (2014) 0.80
Genetically encoded optical activation of DNA recombination in human cells. Chem Commun (Camb) (2016) 0.79
Site-specific promoter caging enables optochemical gene activation in cells and animals. J Am Chem Soc (2014) 0.78
Digital and analog gene circuits for biotechnology. Biotechnol J (2014) 0.78
A Panel of Protease-Responsive RNA Polymerases Respond to Biochemical Signals by Production of Defined RNA Outputs in Live Cells. J Am Chem Soc (2015) 0.76
Genetic code expansion as a tool to study regulatory processes of transcription. Front Chem (2014) 0.75
Small-molecule control of protein function through Staudinger reduction. Nat Chem (2016) 0.75
A Light-Controlled TLR4 Agonist and Selectable Activation of Cell Subpopulations. Chembiochem (2015) 0.75
Optical Control of DNA Helicase Function through Genetic Code Expansion. Chembiochem (2017) 0.75
Optogenetic Modulation of Intracellular Signalling and Transcription: Focus on Neuronal Plasticity. J Exp Neurosci (2017) 0.75
Shining Light on the Sprout of Life: Optogenetics Applications in Stem Cell Research and Therapy. J Membr Biol (2016) 0.75
A system for stable expression of short interfering RNAs in mammalian cells. Science (2002) 35.21
Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev (2002) 10.71
A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci U S A (2002) 8.57
RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc Natl Acad Sci U S A (2002) 6.84
Identification of essential genes in cultured mammalian cells using small interfering RNAs. J Cell Sci (2001) 6.66
Adding new chemistries to the genetic code. Annu Rev Biochem (2010) 6.32
Optical control of mammalian endogenous transcription and epigenetic states. Nature (2013) 6.09
Stable suppression of gene expression by RNAi in mammalian cells. Proc Natl Acad Sci U S A (2002) 5.05
Molecular biology. RNA interference. Science (2000) 4.87
Rapid blue-light-mediated induction of protein interactions in living cells. Nat Methods (2010) 4.70
Biologically active molecules with a "light switch". Angew Chem Int Ed Engl (2006) 3.92
Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase. J Mol Biol (1984) 3.79
Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing. Antisense Nucleic Acid Drug Dev (2003) 3.46
Expanding the genetic code. Annu Rev Biophys Biomol Struct (2006) 3.36
The structural mechanism of translocation and helicase activity in T7 RNA polymerase. Cell (2004) 3.33
Induction of protein-protein interactions in live cells using light. Nat Biotechnol (2009) 3.16
Functional analysis of human microtubule-based motor proteins, the kinesins and dyneins, in mitosis/cytokinesis using RNA interference. Mol Biol Cell (2005) 3.04
Illuminating the chemistry of life: design, synthesis, and applications of "caged" and related photoresponsive compounds. ACS Chem Biol (2009) 2.67
Light-activated kinases enable temporal dissection of signaling networks in living cells. J Am Chem Soc (2011) 2.22
Control of protein phosphorylation with a genetically encoded photocaged amino acid. Nat Chem Biol (2007) 1.99
Light-controlled tools. Angew Chem Int Ed Engl (2012) 1.96
Genetically encoded photocontrol of protein localization in mammalian cells. J Am Chem Soc (2010) 1.94
The promise of optogenetics in cell biology: interrogating molecular circuits in space and time. Nat Methods (2010) 1.87
Expanding the genetic code for biological studies. Chem Biol (2009) 1.85
Rapid mutagenesis and purification of phage RNA polymerases. Protein Expr Purif (1997) 1.84
Designer proteins: applications of genetic code expansion in cell biology. Nat Rev Mol Cell Biol (2012) 1.72
Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors. J Am Chem Soc (2012) 1.69
A genetically encoded photocaged amino acid. J Am Chem Soc (2004) 1.65
Photochemical control of biological processes. Org Biomol Chem (2006) 1.64
Approaches for chemically synthesized siRNA and vector-mediated RNAi. FEBS Lett (2005) 1.58
Mitotic functions of kinesin-5. Semin Cell Dev Biol (2010) 1.56
A genetically encoded photocaged tyrosine. Angew Chem Int Ed Engl (2006) 1.56
Beyond the canonical 20 amino acids: expanding the genetic lexicon. J Biol Chem (2010) 1.53
Visualizing polynucleotide polymerase machines at work. EMBO J (2006) 1.52
Optogenetic control of cells and circuits. Annu Rev Cell Dev Biol (2011) 1.40
An expanding genetic code. Methods (2005) 1.35
Principles and applications of the photochemical control of cellular processes. Chembiochem (2010) 1.33
Light activation as a method of regulating and studying gene expression. Curr Opin Chem Biol (2009) 1.25
Light-activated Cre recombinase as a tool for the spatial and temporal control of gene function in mammalian cells. ACS Chem Biol (2009) 1.23
Endocytosis provides a major alternative pathway for lysosomal biogenesis in kidney proximal tubular cells. Proc Natl Acad Sci U S A (2007) 1.21
A genetically encoded photocaged Nepsilon-methyl-L-lysine. Mol Biosyst (2010) 1.17
Asp537, Asp812 are essential and Lys631, His811 are catalytically significant in bacteriophage T7 RNA polymerase activity. J Mol Biol (1992) 1.16
Developmental gene regulatory networks in the zebrafish embryo. Biochim Biophys Acta (2008) 1.12
A genetically encoded epsilon-N-methyl lysine in mammalian cells. Chembiochem (2010) 1.12
The evolution of flavin-binding photoreceptors: an ancient chromophore serving trendy blue-light sensors. Annu Rev Plant Biol (2011) 1.11
Poleward transport of TPX2 in the mammalian mitotic spindle requires dynein, Eg5, and microtubule flux. Mol Biol Cell (2010) 1.08
Short hairpin RNA-mediated gene silencing. Methods Mol Biol (2013) 1.06
Photocontrol of tyrosine phosphorylation in mammalian cells via genetic encoding of photocaged tyrosine. J Am Chem Soc (2012) 1.02
Building developmental gene regulatory networks. Birth Defects Res C Embryo Today (2009) 1.02
Recent advances in genetic code engineering in Escherichia coli. Curr Opin Biotechnol (2012) 1.01
Common structural features of nucleic acid polymerases. Bioessays (2002) 1.00
Light-activated proteins. Curr Opin Chem Biol (1999) 0.98
Rewiring translation - Genetic code expansion and its applications. FEBS Lett (2012) 0.96
Photocaged t7 RNA polymerase for the light activation of transcription and gene function in pro- and eukaryotic cells. Chembiochem (2010) 0.94
Light-controlled synthetic gene circuits. Curr Opin Chem Biol (2012) 0.94
Reprogramming the genetic code: from triplet to quadruplet codes. Angew Chem Int Ed Engl (2012) 0.93
Lys631 residue in the active site of the bacteriophage T7 RNA polymerase. Affinity labeling and site-directed mutagenesis. Eur J Biochem (1991) 0.86
Light-activated gene editing with a photocaged zinc-finger nuclease. Angew Chem Int Ed Engl (2011) 0.83
Temporal and spatial control of gene expression in early embryos of farm animals. Reprod Fertil Dev (2007) 0.82
Pre-steady-state and steady-state kinetic studies on transcription initiation catalyzed by T7 RNA polymerase and its active-site mutants K631R and Y639F. Biochemistry (1998) 0.82
Knock down of gfp and no tail expression in zebrafish embryo by in vivo-transcribed short hairpin RNA with T7 plasmid system. J Biomed Sci (2007) 0.81
Chemical mutagenesis: selective post-expression interconversion of protein amino acid residues. Curr Opin Chem Biol (2010) 0.78
shRNA driven by Pol II/T7 dual-promoter system effectively induce cell-specific RNA interference in mammalian cells. Biochem Biophys Res Commun (2007) 0.76
Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome. Nature (2010) 3.69
Genetically encoding N(epsilon)-acetyllysine in recombinant proteins. Nat Chem Biol (2008) 3.68
A method for genetically installing site-specific acetylation in recombinant histones defines the effects of H3 K56 acetylation. Mol Cell (2009) 3.11
Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion. Nat Biotechnol (2007) 2.51
Light-activated kinases enable temporal dissection of signaling networks in living cells. J Am Chem Soc (2011) 2.22
A network of orthogonal ribosome x mRNA pairs. Nat Chem Biol (2005) 2.20
Substrate recognition by the AAA+ chaperone ClpB. Nat Struct Mol Biol (2004) 2.19
Expanding the genetic code of an animal. J Am Chem Soc (2011) 2.09
Engineered diubiquitin synthesis reveals Lys29-isopeptide specificity of an OTU deubiquitinase. Nat Chem Biol (2010) 2.09
Genetic encoding and labeling of aliphatic azides and alkynes in recombinant proteins via a pyrrolysyl-tRNA Synthetase/tRNA(CUA) pair and click chemistry. J Am Chem Soc (2009) 2.06
Genetic Encoding of bicyclononynes and trans-cyclooctenes for site-specific protein labeling in vitro and in live mammalian cells via rapid fluorogenic Diels-Alder reactions. J Am Chem Soc (2012) 2.03
Small-molecule inhibitors of microrna miR-21 function. Angew Chem Int Ed Engl (2008) 2.00
Genetically encoded photocontrol of protein localization in mammalian cells. J Am Chem Soc (2010) 1.94
Adding amino acids with novel reactivity to the genetic code of Saccharomyces cerevisiae. J Am Chem Soc (2003) 1.93
Cellular incorporation of unnatural amino acids and bioorthogonal labeling of proteins. Chem Rev (2014) 1.91
Designer proteins: applications of genetic code expansion in cell biology. Nat Rev Mol Cell Biol (2012) 1.72
A genetically encoded fluorescent amino acid. Proc Natl Acad Sci U S A (2006) 1.66
DAXX envelops a histone H3.3-H4 dimer for H3.3-specific recognition. Nature (2012) 1.65
A genetically encoded photocaged amino acid. J Am Chem Soc (2004) 1.65
CPSF6 defines a conserved capsid interface that modulates HIV-1 replication. PLoS Pathog (2012) 1.64
Photochemical control of biological processes. Org Biomol Chem (2006) 1.64
Genetically encoding N(epsilon)-methyl-L-lysine in recombinant histones. J Am Chem Soc (2009) 1.60
Functional epitopes at the ribosome subunit interface. Nat Chem Biol (2006) 1.59
An ankyrin-repeat ubiquitin-binding domain determines TRABID's specificity for atypical ubiquitin chains. Nat Struct Mol Biol (2011) 1.55
Bioorthogonal reactions for labeling proteins. ACS Chem Biol (2014) 1.54
Expanding the genetic code of yeast for incorporation of diverse unnatural amino acids via a pyrrolysyl-tRNA synthetase/tRNA pair. J Am Chem Soc (2010) 1.53
Small molecule modifiers of microRNA miR-122 function for the treatment of hepatitis C virus infection and hepatocellular carcinoma. J Am Chem Soc (2010) 1.53
Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains. Proc Natl Acad Sci U S A (2011) 1.52
Photo-cross-linking interacting proteins with a genetically encoded benzophenone. Nat Methods (2005) 1.49
Synthesis of orthogonal transcription-translation networks. Proc Natl Acad Sci U S A (2009) 1.46
Genetically encoded 1,2-aminothiols facilitate rapid and site-specific protein labeling via a bio-orthogonal cyanobenzothiazole condensation. J Am Chem Soc (2011) 1.42
Expanding the genetic code of Drosophila melanogaster. Nat Chem Biol (2012) 1.41
Gene silencing in mammalian cells with light-activated antisense agents. Chembiochem (2008) 1.33
Recent advances in the photochemical control of protein function. Trends Biotechnol (2010) 1.32
Traceless and site-specific ubiquitination of recombinant proteins. J Am Chem Soc (2011) 1.29
De novo generation of mutually orthogonal aminoacyl-tRNA synthetase/tRNA pairs. J Am Chem Soc (2010) 1.28
Photochemical DNA activation. Org Lett (2007) 1.25
Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP. J Am Chem Soc (2010) 1.23
Light-activated Cre recombinase as a tool for the spatial and temporal control of gene function in mammalian cells. ACS Chem Biol (2009) 1.23
Development of a robust and high throughput method for profiling N-linked glycans derived from plasma glycoproteins by NanoLC-FTICR mass spectrometry. J Proteome Res (2009) 1.19
A view to a kill: ligands for Bcl-2 family proteins. Curr Opin Chem Biol (2002) 1.18
Acetylation of lysine 120 of p53 endows DNA-binding specificity at effective physiological salt concentration. Proc Natl Acad Sci U S A (2011) 1.16
Cellular logic with orthogonal ribosomes. J Am Chem Soc (2005) 1.12
Generating permissive site-specific unnatural aminoacyl-tRNA synthetases. Biochemistry (2010) 1.10
Activation and deactivation of DNAzyme and antisense function with light for the photochemical regulation of gene expression in mammalian cells. J Am Chem Soc (2010) 1.09
Genetically encoding protein oxidative damage. J Am Chem Soc (2008) 1.08
Efficient multisite unnatural amino acid incorporation in mammalian cells via optimized pyrrolysyl tRNA synthetase/tRNA expression and engineered eRF1. J Am Chem Soc (2014) 1.05
Genetically directing ɛ-N, N-dimethyl-L-lysine in recombinant histones. Chem Biol (2010) 1.05
Photochemical activation of protein expression in bacterial cells. Angew Chem Int Ed Engl (2007) 1.04
Light-triggered polymerase chain reaction. Chem Commun (Camb) (2007) 1.04
The human mitochondrial tRNAMet: structure/function relationship of a unique modification in the decoding of unconventional codons. J Mol Biol (2010) 1.03
Tricyclic alkaloid core structures assembled by a cyclotrimerization-coupled intramolecular nucleophilic substitution reaction. Org Lett (2010) 1.03
Photocontrol of tyrosine phosphorylation in mammalian cells via genetic encoding of photocaged tyrosine. J Am Chem Soc (2012) 1.02
Acetylation regulates cyclophilin A catalysis, immunosuppression and HIV isomerization. Nat Chem Biol (2010) 1.02
Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet. Nucleic Acids Res (2008) 1.02
A dual role of H4K16 acetylation in the establishment of yeast silent chromatin. EMBO J (2011) 1.00
High-throughput luciferase reporter assay for small-molecule inhibitors of microRNA function. J Biomol Screen (2012) 0.98
Light-activated deoxyguanosine: photochemical regulation of peroxidase activity. Mol Biosyst (2008) 0.98
Photochemical hammerhead ribozyme activation. Bioorg Med Chem Lett (2006) 0.97
Concerted, rapid, quantitative, and site-specific dual labeling of proteins. J Am Chem Soc (2014) 0.97
Genetic encoding of photocaged cysteine allows photoactivation of TEV protease in live mammalian cells. J Am Chem Soc (2014) 0.96
Microwave activation of enzymatic catalysis. J Am Chem Soc (2008) 0.96
Optical control of protein function through unnatural amino acid mutagenesis and other optogenetic approaches. ACS Chem Biol (2014) 0.95
DNA computation in mammalian cells: microRNA logic operations. J Am Chem Soc (2013) 0.95
A light-activated DNA polymerase. Angew Chem Int Ed Engl (2009) 0.95
Photocaged t7 RNA polymerase for the light activation of transcription and gene function in pro- and eukaryotic cells. Chembiochem (2010) 0.94
Light-controlled synthetic gene circuits. Curr Opin Chem Biol (2012) 0.94
DNA computation: a photochemically controlled AND gate. J Am Chem Soc (2012) 0.93
Reprogramming the genetic code: from triplet to quadruplet codes. Angew Chem Int Ed Engl (2012) 0.93
Photochemical control of DNA decoy function enables precise regulation of nuclear factor κB activity. J Am Chem Soc (2011) 0.93
Engineering Escherichia coli heat-resistance by synthetic gene amplification. Protein Eng Des Sel (2008) 0.92
Light-activation of gene function in mammalian cells via ribozymes. Chem Commun (Camb) (2008) 0.92
Light-regulated RNA-small molecule interactions. Chembiochem (2008) 0.92
Efficient and rapid C. elegans transgenesis by bombardment and hygromycin B selection. PLoS One (2013) 0.91
Genetically encoded optochemical probes for simultaneous fluorescence reporting and light activation of protein function with two-photon excitation. J Am Chem Soc (2014) 0.91
A general approach to chemo- and regioselective cyclotrimerization reactions. Angew Chem Int Ed Engl (2007) 0.89
Site-specific incorporation of fluorotyrosines into proteins in Escherichia coli by photochemical disguise. Biochemistry (2010) 0.89
Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes. Chem Commun (Camb) (2014) 0.88
Sequential gene silencing using wavelength-selective caged morpholino oligonucleotides. Angew Chem Int Ed Engl (2014) 0.88
Regulation of transcription through light-activation and light-deactivation of triplex-forming oligonucleotides in mammalian cells. ACS Chem Biol (2012) 0.88
Optochemical control of deoxyoligonucleotide function via a nucleobase-caging approach. Acc Chem Res (2013) 0.87
Two rapid catalyst-free click reactions for in vivo protein labeling of genetically encoded strained alkene/alkyne functionalities. Bioconjug Chem (2014) 0.86
Spatiotemporal control of microRNA function using light-activated antagomirs. Mol Biosyst (2012) 0.85
A photoactivatable small-molecule inhibitor for light-controlled spatiotemporal regulation of Rho kinase in live embryos. Development (2012) 0.85
Evolved orthogonal ribosome purification for in vitro characterization. Nucleic Acids Res (2010) 0.84
A cyclotrimerization route to cannabinoids. Org Lett (2008) 0.84
Light-activated gene editing with a photocaged zinc-finger nuclease. Angew Chem Int Ed Engl (2011) 0.83
Genetic encoding of caged cysteine and caged homocysteine in bacterial and mammalian cells. Chembiochem (2014) 0.83
Photochemical regulation of restriction endonuclease activity. Chembiochem (2009) 0.82
Expanding nucleic acid function in vitro and in vivo. Curr Opin Chem Biol (2006) 0.82
The effect of microwave irradiation on DNA hybridization. Org Biomol Chem (2009) 0.82
Optochemical control of RNA interference in mammalian cells. Nucleic Acids Res (2013) 0.82