Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs.

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Published in Sci Rep on August 21, 2015

Authors

Xianlong Wang1, Jinwei Zhou2, Chunwei Cao1, Jiaojiao Huang1,3, Tang Hai1, Yanfang Wang4, Qiantao Zheng1,3, Hongyong Zhang1,3, Guosong Qin1, Xiangnan Miao1, Hongmei Wang1,3, Suizhong Cao2, Qi Zhou1,3, Jianguo Zhao1,3

Author Affiliations

1: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
2: College of Veterinary Medicine, Sichuan Agriculture University, Ya'an, Sichuan 625014, China.
3: University of Chinese Academy of Sciences, Beijing 100049, China.
4: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

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