Published in Nature on April 03, 2005
RNA-guided human genome engineering via Cas9. Science (2013) 48.29
A TALE nuclease architecture for efficient genome editing. Nat Biotechnol (2010) 26.47
Genome editing with engineered zinc finger nucleases. Nat Rev Genet (2010) 18.88
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases. Nat Biotechnol (2008) 14.75
Targeting DNA double-strand breaks with TAL effector nucleases. Genetics (2010) 14.49
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat Biotechnol (2008) 14.20
Development and applications of CRISPR-Cas9 for genome engineering. Cell (2014) 13.50
Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol (2009) 12.40
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol (2013) 11.58
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell (2008) 11.24
Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol (2008) 10.29
Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells. Cell Stem Cell (2009) 8.97
Distinct factors control histone variant H3.3 localization at specific genomic regions. Cell (2010) 8.79
Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly. Genome Res (2009) 8.69
A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity. Nucleic Acids Res (2011) 7.93
Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos. Cell Res (2013) 7.41
TALENs: a widely applicable technology for targeted genome editing. Nat Rev Mol Cell Biol (2012) 7.01
Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection. Nat Methods (2011) 6.90
Cas9 as a versatile tool for engineering biology. Nat Methods (2013) 6.87
A transcription activator-like effector toolbox for genome engineering. Nat Protoc (2012) 6.81
Rapid mutation of endogenous zebrafish genes using zinc finger nucleases made by Oligomerized Pool ENgineering (OPEN). PLoS One (2009) 6.67
Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases. Proc Natl Acad Sci U S A (2008) 6.59
Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations. Cell (2011) 6.56
Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo. Nat Biotechnol (2010) 6.55
An unbiased genome-wide analysis of zinc-finger nuclease specificity. Nat Biotechnol (2011) 6.44
Targeted chromosomal deletions in human cells using zinc finger nucleases. Genome Res (2009) 6.24
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases. Genome Res (2013) 6.19
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures. Nat Methods (2010) 6.00
Induction and repair of zinc-finger nuclease-targeted double-strand breaks in Caenorhabditis elegans somatic cells. Proc Natl Acad Sci U S A (2006) 6.00
Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases. Proc Natl Acad Sci U S A (2007) 5.93
High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases. Nat Methods (2011) 5.68
Targeted integration in rat and mouse embryos with zinc-finger nucleases. Nat Biotechnol (2010) 5.60
Dynamics of DNA damage response proteins at DNA breaks: a focus on protein modifications. Genes Dev (2011) 5.60
Efficient TALEN-mediated gene knockout in livestock. Proc Natl Acad Sci U S A (2012) 4.99
Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells. Nature (2011) 4.92
A common BIM deletion polymorphism mediates intrinsic resistance and inferior responses to tyrosine kinase inhibitors in cancer. Nat Med (2012) 4.90
In vivo genome editing restores haemostasis in a mouse model of haemophilia. Nature (2011) 4.81
High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases. Proc Natl Acad Sci U S A (2010) 4.78
Precise genome modification in the crop species Zea mays using zinc-finger nucleases. Nature (2009) 4.71
Genome engineering with zinc-finger nucleases. Genetics (2011) 4.66
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods (2013) 4.61
Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A (2013) 4.57
Efficient gene targeting in Drosophila with zinc-finger nucleases. Genetics (2006) 4.47
Computational redesign of endonuclease DNA binding and cleavage specificity. Nature (2006) 4.06
Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome. Genome Res (2010) 3.94
Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases. Proc Natl Acad Sci U S A (2010) 3.90
Genome editing with modularly assembled zinc-finger nucleases. Nat Methods (2010) 3.79
Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. Proc Natl Acad Sci U S A (2008) 3.70
ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool. Nucleic Acids Res (2010) 3.59
Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool. Nucleic Acids Res (2007) 3.47
Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells. Nucleic Acids Res (2005) 3.47
Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs. Nucleic Acids Res (2012) 3.41
Surrogate reporters for enrichment of cells with nuclease-induced mutations. Nat Methods (2011) 3.22
Human T regulatory cell therapy: take a billion or so and call me in the morning. Immunity (2009) 3.21
Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases. PLoS One (2010) 3.09
Engineering lymphocyte subsets: tools, trials and tribulations. Nat Rev Immunol (2009) 3.06
Precision genome engineering with programmable DNA-nicking enzymes. Genome Res (2012) 3.05
Expanding or restricting the target site repertoire of zinc-finger nucleases: the inter-domain linker as a major determinant of target site selectivity. Mol Ther (2008) 3.02
Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Nat Protoc (2009) 3.01
Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA. Science (2014) 2.93
Rapid and efficient clathrin-mediated endocytosis revealed in genome-edited mammalian cells. Nat Cell Biol (2011) 2.79
Optimization of scarless human stem cell genome editing. Nucleic Acids Res (2013) 2.78
Chromosomal translocations induced at specified loci in human stem cells. Proc Natl Acad Sci U S A (2009) 2.73
Concerning RNA-guided gene drives for the alteration of wild populations. Elife (2014) 2.69
Targeted mutagenesis in zebrafish using customized zinc-finger nucleases. Nat Protoc (2009) 2.67
Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases. J Mol Biol (2010) 2.65
TALEN-mediated precise genome modification by homologous recombination in zebrafish. Nat Methods (2013) 2.64
Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases. Proc Natl Acad Sci U S A (2011) 2.61
Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology. Nucleic Acids Res (2010) 2.61
Targeted genome editing in human repopulating haematopoietic stem cells. Nature (2014) 2.49
Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus. Sci Rep (2013) 2.48
Therapeutic genome editing: prospects and challenges. Nat Med (2015) 2.42
A combinatorial approach to create artificial homing endonucleases cleaving chosen sequences. Nucleic Acids Res (2006) 2.38
The molecular profile of microglia under the influence of glioma. Neuro Oncol (2012) 2.38
DNA ligase III promotes alternative nonhomologous end-joining during chromosomal translocation formation. PLoS Genet (2011) 2.22
Attenuation of zinc finger nuclease toxicity by small-molecule regulation of protein levels. PLoS Genet (2009) 2.18
Binding of two zinc finger nuclease monomers to two specific sites is required for effective double-strand DNA cleavage. Biochem Biophys Res Commun (2005) 2.15
Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease. Nucleic Acids Res (2009) 2.14
Ex vivo gene transfer and correction for cell-based therapies. Nat Rev Genet (2011) 2.13
Correction of the sickle cell disease mutation in human hematopoietic stem/progenitor cells. Blood (2015) 2.00
Advances in targeted genome editing. Curr Opin Chem Biol (2012) 1.97
Repair of strand breaks by homologous recombination. Cold Spring Harb Perspect Biol (2013) 1.96
Synthesis of programmable integrases. Proc Natl Acad Sci U S A (2009) 1.94
Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion. Nucleic Acids Res (2010) 1.92
Efficient targeted gene disruption in the soma and germ line of the frog Xenopus tropicalis using engineered zinc-finger nucleases. Proc Natl Acad Sci U S A (2011) 1.90
Modular system for the construction of zinc-finger libraries and proteins. Nat Protoc (2010) 1.87
Editing T cell specificity towards leukemia by zinc finger nucleases and lentiviral gene transfer. Nat Med (2012) 1.87
TALEN-based gene correction for epidermolysis bullosa. Mol Ther (2013) 1.84
Targeting the absence: homozygous DNA deletions as immutable signposts for cancer therapy. Proc Natl Acad Sci U S A (2007) 1.84
Genome editing of human embryonic stem cells and induced pluripotent stem cells with zinc finger nucleases for cellular imaging. Circ Res (2012) 1.84
Zinc finger protein-dependent and -independent contributions to the in vivo off-target activity of zinc finger nucleases. Nucleic Acids Res (2010) 1.81
The p400 ATPase regulates nucleosome stability and chromatin ubiquitination during DNA repair. J Cell Biol (2010) 1.80
Site-specific genome editing in Plasmodium falciparum using engineered zinc-finger nucleases. Nat Methods (2012) 1.79
Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy. Curr Gene Ther (2011) 1.76
Translating dosage compensation to trisomy 21. Nature (2013) 1.75
Site-directed transposon integration in human cells. Nucleic Acids Res (2007) 1.74
Computer design of obligate heterodimer meganucleases allows efficient cutting of custom DNA sequences. Nucleic Acids Res (2008) 1.74
The triple helix: 50 years later, the outcome. Nucleic Acids Res (2008) 1.73
Efficient immunoglobulin gene disruption and targeted replacement in rabbit using zinc finger nucleases. PLoS One (2011) 1.69
Genome-scale engineering for systems and synthetic biology. Mol Syst Biol (2013) 1.68
A TALE nuclease architecture for efficient genome editing. Nat Biotechnol (2010) 26.47
Genome editing with engineered zinc finger nucleases. Nat Rev Genet (2010) 18.88
An improved zinc-finger nuclease architecture for highly specific genome editing. Nat Biotechnol (2007) 17.80
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases. Nat Biotechnol (2008) 14.75
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat Biotechnol (2008) 14.20
Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol (2009) 12.40
Knockout rats via embryo microinjection of zinc-finger nucleases. Science (2009) 12.33
Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol (2011) 11.76
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell (2008) 11.24
Targeted genome editing across species using ZFNs and TALENs. Science (2011) 9.84
Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery. Nat Biotechnol (2007) 9.23
Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells. Cell Stem Cell (2009) 8.97
Distinct factors control histone variant H3.3 localization at specific genomic regions. Cell (2010) 8.79
Knockout rats generated by embryo microinjection of TALENs. Nat Biotechnol (2011) 8.45
Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV. N Engl J Med (2014) 7.64
A rapid and general assay for monitoring endogenous gene modification. Methods Mol Biol (2010) 6.77
Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases. Proc Natl Acad Sci U S A (2008) 6.59
Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations. Cell (2011) 6.56
Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo. Nat Biotechnol (2010) 6.55
An unbiased genome-wide analysis of zinc-finger nuclease specificity. Nat Biotechnol (2011) 6.44
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures. Nat Methods (2010) 6.00
Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases. Proc Natl Acad Sci U S A (2007) 5.93
Histone deimination antagonizes arginine methylation. Cell (2004) 5.19
Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells. Nature (2011) 4.92
In vivo genome editing restores haemostasis in a mouse model of haemophilia. Nature (2011) 4.81
Precise genome modification in the crop species Zea mays using zinc-finger nucleases. Nature (2009) 4.71
Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Nat Protoc (2006) 4.16
Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome. Genome Res (2010) 3.94
Transient cold shock enhances zinc-finger nuclease-mediated gene disruption. Nat Methods (2010) 3.59
Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells. Nucleic Acids Res (2005) 3.47
Telomeric DNA damage is irreparable and causes persistent DNA-damage-response activation. Nat Cell Biol (2012) 3.03
Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor. Cell (2012) 2.87
Comparison of zinc finger nucleases for use in gene targeting in mammalian cells. Mol Ther (2008) 2.87
Induction of angiogenesis in a mouse model using engineered transcription factors. Nat Med (2002) 2.81
Rapid and efficient clathrin-mediated endocytosis revealed in genome-edited mammalian cells. Nat Cell Biol (2011) 2.79
An erythroid enhancer of BCL11A subject to genetic variation determines fetal hemoglobin level. Science (2013) 2.71
Drug discovery with engineered zinc-finger proteins. Nat Rev Drug Discov (2003) 2.63
Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases. Proc Natl Acad Sci U S A (2011) 2.61
Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology. Nucleic Acids Res (2010) 2.61
TRF2 associates with DREF and directs promoter-selective gene expression in Drosophila. Nature (2002) 2.48
Targeted transgene integration in plant cells using designed zinc finger nucleases. Plant Mol Biol (2008) 2.40
Site-specific integration and tailoring of cassette design for sustainable gene transfer. Nat Methods (2011) 2.30
Zinc-finger protein-targeted gene regulation: genomewide single-gene specificity. Proc Natl Acad Sci U S A (2003) 2.24
Generation of a triple-gene knockout mammalian cell line using engineered zinc-finger nucleases. Biotechnol Bioeng (2010) 2.23
DNA ligase III promotes alternative nonhomologous end-joining during chromosomal translocation formation. PLoS Genet (2011) 2.22
Attenuation of zinc finger nuclease toxicity by small-molecule regulation of protein levels. PLoS Genet (2009) 2.18
Human SMC5/6 complex promotes sister chromatid homologous recombination by recruiting the SMC1/3 cohesin complex to double-strand breaks. EMBO J (2006) 2.13
MOF and histone H4 acetylation at lysine 16 are critical for DNA damage response and double-strand break repair. Mol Cell Biol (2010) 2.03
Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme. Genome Res (2012) 2.02