A new design of a lentiviral shRNA vector with inducible co-expression of ARGONAUTE 2 for enhancing gene silencing efficiency.

PubWeight™: 0.75‹?›

🔗 View Article (PMID 26649169)

Published in Cell Biosci on December 08, 2015

Authors

Jiening He1, Lian Huang1, Huiling Qiu1, Jiexuan Li1, Lan Luo1, Yanjiao Li1, Shengli Tian1, Kang Kang2, Jun Luo3, Lin Liu4, Deming Gou1

Author Affiliations

1: Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences, Shenzhen University, Nanhai Ave 3688, Shenzhen, 518060 Guangdong China.
2: Department of Physiology, Shenzhen University Health Science Center, Shenzhen University, Shenzhen, 518060 Guangdong China.
3: College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi China.
4: Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 74078 USA.

Articles cited by this

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature (1998) 72.56

A system for stable expression of short interfering RNAs in mammalian cells. Science (2002) 35.21

Argonaute2 is the catalytic engine of mammalian RNAi. Science (2004) 22.86

A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature (2007) 18.38

Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. Genes Dev (2003) 17.64

Argonaute proteins: key players in RNA silencing. Nat Rev Mol Cell Biol (2008) 8.96

A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature (2004) 8.67

A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci U S A (2002) 8.57

Second-generation shRNA libraries covering the mouse and human genomes. Nat Genet (2005) 8.23

Cloning of short hairpin RNAs for gene knockdown in mammalian cells. Nat Methods (2004) 2.62

Locked nucleic acid (LNA) mediated improvements in siRNA stability and functionality. Nucleic Acids Res (2005) 2.20

Argonaute proteins are key determinants of RNAi efficacy, toxicity, and persistence in the adult mouse liver. J Clin Invest (2010) 1.91

A small molecule enhances RNA interference and promotes microRNA processing. Nat Biotechnol (2008) 1.80

Pol II-expressed shRNA knocks down Sod2 gene expression and causes phenotypes of the gene knockout in mice. PLoS Genet (2006) 1.49

Coexpression of Argonaute-2 enhances RNA interference toward perfect match binding sites. Proc Natl Acad Sci U S A (2008) 1.49

Defining the optimal parameters for hairpin-based knockdown constructs. RNA (2007) 1.48

dsRNA binding properties of RDE-4 and TRBP reflect their distinct roles in RNAi. J Mol Biol (2008) 1.39

Gene silencing in alveolar type II cells using cell-specific promoter in vitro and in vivo. Nucleic Acids Res (2004) 1.33

Limiting Ago protein restricts RNAi and microRNA biogenesis during early development in Xenopus laevis. Genes Dev (2011) 1.30

A novel approach for the construction of multiple shRNA expression vectors. J Gene Med (2007) 1.21

Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell lines. Nucleic Acids Res (2013) 1.20

Minimal-length short hairpin RNAs: the relationship of structure and RNAi activity. RNA (2009) 1.16

Right- and left-loop short shRNAs have distinct and unusual mechanisms of gene silencing. Nucleic Acids Res (2012) 1.07

Co-expression of Argonaute2 Enhances Short Hairpin RNA-induced RNA Interference in Xenopus CNS Neurons In Vivo. Front Neurosci (2009) 0.86

Embedding siRNA sequences targeting apolipoprotein B100 in shRNA and miRNA scaffolds results in differential processing and in vivo efficacy. Mol Ther (2012) 0.86

Lentivirus-AIMP2-DX2 shRNA suppresses cell proliferation by regulating Akt1 signaling pathway in the lungs of AIMP2⁺/⁻ mice. J Aerosol Med Pulm Drug Deliv (2013) 0.81

Design and chemical modification of synthetic short shRNAs as potent RNAi triggers. Methods Mol Biol (2013) 0.78

RNAi therapeutics: the teenage years. Biotechniques (2012) 0.78