Published in Mar Drugs on March 21, 2016
Environmental genome shotgun sequencing of the Sargasso Sea. Science (2004) 45.23
Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod (2012) 13.27
Metagenomics: application of genomics to uncultured microorganisms. Microbiol Mol Biol Rev (2004) 10.67
Rapid evolution of a protein in vitro by DNA shuffling. Nature (1994) 9.67
Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol (1998) 9.46
Bioactive microbial metabolites. J Antibiot (Tokyo) (2005) 8.75
Microbial sciences: the superficial life of microbes. Nature (2006) 3.45
The metagenomics of soil. Nat Rev Microbiol (2005) 3.24
Ocean plankton. Structure and function of the global ocean microbiome. Science (2015) 3.08
Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125. Genome Res (2005) 2.89
Microbial drug discovery: 80 years of progress. J Antibiot (Tokyo) (2009) 2.71
Marine natural products. Nat Prod Rep (2011) 2.69
Metagenomics: advances in ecology and biotechnology. FEMS Microbiol Lett (2005) 2.45
Metagenomics and industrial applications. Nat Rev Microbiol (2005) 2.42
Directed evolution drives the next generation of biocatalysts. Nat Chem Biol (2009) 2.41
Biotechnological prospects from metagenomics. Curr Opin Biotechnol (2003) 2.32
Metagenomic analyses: past and future trends. Appl Environ Microbiol (2010) 2.21
DNA shuffling method for generating highly recombined genes and evolved enzymes. Nat Biotechnol (2001) 2.21
The re-emergence of natural products for drug discovery in the genomics era. Nat Rev Drug Discov (2015) 2.15
Biodiversity as a source of innovation in biotechnology. Annu Rev Microbiol (1992) 2.11
Ocean plankton. Patterns and ecological drivers of ocean viral communities. Science (2015) 2.00
Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes. Nat Protoc (2007) 1.97
Synthesis of methyl halides from biomass using engineered microbes. J Am Chem Soc (2009) 1.90
Microbial enzymes mined from the Urania deep-sea hypersaline anoxic basin. Chem Biol (2005) 1.82
Phylogenetic diversity and metabolic potential revealed in a glacier ice metagenome. Appl Environ Microbiol (2009) 1.76
Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently. Chem Soc Rev (2015) 1.74
Microbial oceanography: paradigms, processes and promise. Nat Rev Microbiol (2007) 1.72
Chitinases from uncultured marine microorganisms. Appl Environ Microbiol (1999) 1.67
Functional metagenomics for enzyme discovery: challenges to efficient screening. Curr Opin Biotechnol (2009) 1.64
Recombinant approaches for accessing biodiversity. Nat Biotechnol (1997) 1.58
Marine natural products: a new wave of drugs? Future Med Chem (2011) 1.57
Metagenomics--the key to the uncultured microbes. Curr Opin Microbiol (2004) 1.56
Expanding the range of substrate acceptance of enzymes: combinatorial active-site saturation test. Angew Chem Int Ed Engl (2005) 1.54
Directed evolution of an aspartate aminotransferase with new substrate specificities. Proc Natl Acad Sci U S A (1998) 1.54
Expression patterns reveal niche diversification in a marine microbial assemblage. ISME J (2012) 1.53
Industrial biotechnology-a chance at redemption. Nat Biotechnol (2004) 1.47
Chitinase gene sequences retrieved from diverse aquatic habitats reveal environment-specific distributions. Appl Environ Microbiol (2004) 1.47
Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases. Appl Environ Microbiol (2006) 1.46
Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome. Appl Microbiol Biotechnol (2008) 1.41
Expanding small-molecule functional metagenomics through parallel screening of broad-host-range cosmid environmental DNA libraries in diverse proteobacteria. Appl Environ Microbiol (2010) 1.41
Microbial genome mining for accelerated natural products discovery: is a renaissance in the making? J Ind Microbiol Biotechnol (2013) 1.40
Methods for the directed evolution of proteins. Nat Rev Genet (2015) 1.38
Bio-mining the microbial treasures of the ocean: new natural products. Biotechnol Adv (2011) 1.37
Role and significance of beta-glucosidases in the hydrolysis of cellulose for bioethanol production. Bioresour Technol (2012) 1.36
Metagenomic era for biocatalyst identification. Curr Opin Biotechnol (2010) 1.34
Metagenomic approach for the isolation of a novel low-temperature-active lipase from uncultured bacteria of marine sediment. FEMS Microbiol Ecol (2007) 1.34
Marine metagenomics: strategies for the discovery of novel enzymes with biotechnological applications from marine environments. Microb Cell Fact (2008) 1.32
Chiral drugs: an overview. Int J Biomed Sci (2006) 1.31
Flavin-dependent halogenases involved in secondary metabolism in bacteria. Appl Microbiol Biotechnol (2006) 1.29
Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM). J Am Chem Soc (2003) 1.27
Insights from 20 years of bacterial genome sequencing. Funct Integr Genomics (2015) 1.27
Advances in generating functional diversity for directed protein evolution. Curr Opin Chem Biol (2009) 1.23
The search for the ideal biocatalyst. Nat Biotechnol (2002) 1.23
Employing modular polyketide synthase ketoreductases as biocatalysts in the preparative chemoenzymatic syntheses of diketide chiral building blocks. Chem Biol (2011) 1.23
Identification of two novel esterases from a marine metagenomic library derived from South China Sea. Appl Microbiol Biotechnol (2008) 1.21
Marine sponge derived natural products between 2001 and 2010: trends and opportunities for discovery of bioactives. Mar Drugs (2014) 1.19
Genomics of an extreme psychrophile, Psychromonas ingrahamii. BMC Genomics (2008) 1.19
Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods. J Am Chem Soc (2010) 1.18
Chiral toxicology: it's the same thing...only different. Toxicol Sci (2009) 1.17
Cloning and characterization of a beta-glucosidase from marine microbial metagenome with excellent glucose tolerance. J Microbiol Biotechnol (2010) 1.15
What lies underneath: conserving the oceans' genetic resources. Proc Natl Acad Sci U S A (2010) 1.14
Marine biocatalysts: enzymatic features and applications. Mar Drugs (2011) 1.13
A novel family 8 xylanase, functional and physicochemical characterization. J Biol Chem (2002) 1.11
Interrelationships between colonies, biofilms, and planktonic cells of Pseudomonas aeruginosa. J Bacteriol (2007) 1.11
Prospecting for novel lipase genes using PCR. Microbiology (2002) 1.09
Molecular characterization of TEM-type beta-lactamases identified in cold-seep sediments of Edison Seamount (south of Lihir Island, Papua New Guinea). J Microbiol (2005) 1.09
Cultivating the uncultivated: a community genomics perspective. Trends Microbiol (2005) 1.08
Kinetic and structural optimization to catalysis at low temperatures in a psychrophilic cellulase from the Antarctic bacterium Pseudoalteromonas haloplanktis. Biochem J (2004) 1.08
Approaches to capturing and designing biologically active small molecules produced by uncultured microbes. Annu Rev Microbiol (2011) 1.07
Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms. Appl Environ Microbiol (2008) 1.05
Neptuniibacter caesariensis gen. nov., sp. nov., a novel marine genome-sequenced gammaproteobacterium. Int J Syst Evol Microbiol (2007) 1.03
Metagenomic gene discovery: how far have we moved into novel sequence space? Biotechnol J (2009) 1.03
Metagenomic approaches for exploiting uncultivated bacteria as a resource for novel biosynthetic enzymology. Chem Biol (2013) 1.03
Identification of a new subfamily of salt-tolerant esterases from a metagenomic library of tidal flat sediment. Appl Microbiol Biotechnol (2011) 1.02
Characterization and its potential application of two esterases derived from the arctic sediment metagenome. Mar Biotechnol (NY) (2008) 1.02
Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment. FEMS Microbiol Ecol (2010) 1.01
A broader view: microbial enzymes and their relevance in industries, medicine, and beyond. Biomed Res Int (2013) 1.01
Biogeography of the ubiquitous marine bacterium Alteromonas macleodii determined by multilocus sequence analysis. Mol Ecol (2008) 1.01
Cloning and characterization of three epoxide hydrolases from a marine bacterium, Erythrobacter litoralis HTCC2594. Appl Microbiol Biotechnol (2007) 1.01
Functional metagenomic strategies for the discovery of novel enzymes and biosurfactants with biotechnological applications from marine ecosystems. J Appl Microbiol (2011) 1.00
Screening and characterization of a novel fibrinolytic metalloprotease from a metagenomic library. Biotechnol Lett (2007) 0.99
Bioactive peptides and depsipeptides with anticancer potential: sources from marine animals. Mar Drugs (2012) 0.99
Biocatalytic synthesis of chiral alcohols and amino acids for development of pharmaceuticals. Biomolecules (2013) 0.99
Armodafinil and modafinil have substantially different pharmacokinetic profiles despite having the same terminal half-lives: analysis of data from three randomized, single-dose, pharmacokinetic studies. Clin Drug Investig (2009) 0.98
A bacterial laccase from marine microbial metagenome exhibiting chloride tolerance and dye decolorization ability. Appl Microbiol Biotechnol (2010) 0.97
Metagenomic approaches to exploit the biotechnological potential of the microbial consortia of marine sponges. Appl Microbiol Biotechnol (2007) 0.97
A wide host-range metagenomic library from a waste water treatment plant yields a novel alcohol/aldehyde dehydrogenase. Environ Microbiol (2005) 0.97
Rapid evolution of novel traits in microorganisms. Appl Environ Microbiol (2001) 0.97
Purification and characterization of a cold-adapted alpha-amylase produced by Nocardiopsis sp. 7326 isolated from Prydz Bay, Antarctic. Mar Biotechnol (NY) (2007) 0.96
Metagenomic insights into anaerobic metabolism along an Arctic peat soil profile. PLoS One (2013) 0.96
A novel family VIII carboxylesterase derived from a leachate metagenome library exhibits promiscuous beta-lactamase activity on nitrocefin. Appl Microbiol Biotechnol (2009) 0.95
Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library. Proc Natl Acad Sci U S A (2011) 0.95
Cloning of two pectate lyase genes from the marine Antarctic bacterium Pseudoalteromonas haloplanktis strain ANT/505 and characterization of the enzymes. Extremophiles (2001) 0.94
Autotrophic microbe metagenomes and metabolic pathways differentiate adjacent Red Sea brine pools. Sci Rep (2013) 0.94
Isolation identification and biochemical characterization of a novel halo-tolerant lipase from the metagenome of the marine sponge Haliclona simulans. Microb Cell Fact (2012) 0.93
Microbial carbohydrate esterases in cold adapted environments. Gene (2007) 0.93
Biological activities and potential health benefits of bioactive peptides derived from marine organisms. Int J Biol Macromol (2012) 0.91
Recent advances in marine drug research. Biotechnol Adv (2013) 0.91
Biocatalyst development by directed evolution. Bioresour Technol (2012) 0.91
Functional and structural studies of a novel cold-adapted esterase from an Arctic intertidal metagenomic library. Appl Microbiol Biotechnol (2012) 0.90