CRISPR-Mediated VHL Knockout Generates an Improved Model for Metastatic Renal Cell Carcinoma.

PubWeight™: 0.75‹?›

🔗 View Article (PMID 27358011)

Published in Sci Rep on June 30, 2016

Authors

Shiruyeh Schokrpur1, Junhui Hu1,2,3, Diana L Moughon1, Peijun Liu2, Lucia C Lin1, Kip Hermann1, Serghei Mangul4, Wei Guan1,2, Matteo Pellegrini4,5, Hua Xu2, Lily Wu1,6

Author Affiliations

1: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles CA 90095, USA.
2: Department of Urology and Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
3: Department of Paediatric Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
4: Department of Computer Science and Human Genetics, University of California at Los Angeles CA 90095, USA.
5: Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles CA 90095, USA.
6: Department of Urology, David Geffen School of Medicine, University of California at Los Angeles CA 90095, USA.

Articles cited by this

Multiplex genome engineering using CRISPR/Cas systems. Science (2013) 55.53

Safety, activity, and immune correlates of anti-PD-1 antibody in cancer. N Engl J Med (2012) 52.99

A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17

The basics of epithelial-mesenchymal transition. J Clin Invest (2009) 32.82

The cBio cancer genomics portal: an open platform for exploring multidimensional cancer genomics data. Cancer Discov (2012) 26.98

One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell (2013) 24.00

Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation. Science (2001) 23.83

HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing. Science (2001) 22.06

DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol (2013) 19.99

C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation. Cell (2001) 19.75

Integrative analysis of complex cancer genomics and clinical profiles using the cBioPortal. Sci Signal (2013) 19.54

Development and applications of CRISPR-Cas9 for genome engineering. Cell (2014) 13.50

Epithelial-mesenchymal transition and its implications for fibrosis. J Clin Invest (2003) 12.56

Genome-scale CRISPR-Cas9 knockout screening in human cells. Science (2013) 12.36

Identification of the von Hippel-Lindau disease tumor suppressor gene. Science (1993) 11.95

A conserved family of prolyl-4-hydroxylases that modify HIF. Science (2001) 11.50

Differential roles of hypoxia-inducible factor 1alpha (HIF-1alpha) and HIF-2alpha in hypoxic gene regulation. Mol Cell Biol (2003) 8.12

One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell (2013) 7.99

Direct regulation of TWIST by HIF-1alpha promotes metastasis. Nat Cell Biol (2008) 7.37

Contrasting properties of hypoxia-inducible factor 1 (HIF-1) and HIF-2 in von Hippel-Lindau-associated renal cell carcinoma. Mol Cell Biol (2005) 6.44

HIF prolyl-hydroxylase 2 is the key oxygen sensor setting low steady-state levels of HIF-1alpha in normoxia. EMBO J (2003) 6.44

Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods (2014) 5.76

Genomic architecture and evolution of clear cell renal cell carcinomas defined by multiregion sequencing. Nat Genet (2014) 5.74

In vivo genome editing using Staphylococcus aureus Cas9. Nature (2015) 5.26

Sunitinib mediates reversal of myeloid-derived suppressor cell accumulation in renal cell carcinoma patients. Clin Cancer Res (2009) 5.10

HIF-1alpha binding to VHL is regulated by stimulus-sensitive proline hydroxylation. Proc Natl Acad Sci U S A (2001) 5.05

A CXCL1 paracrine network links cancer chemoresistance and metastasis. Cell (2012) 4.89

Dimerization, DNA binding, and transactivation properties of hypoxia-inducible factor 1. J Biol Chem (1996) 4.72

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library. Nat Biotechnol (2013) 4.72

Hypoxia-inducible factor determines sensitivity to inhibitors of mTOR in kidney cancer. Nat Med (2005) 4.44

Epithelial-to-mesenchymal transition is not required for lung metastasis but contributes to chemoresistance. Nature (2015) 4.18

Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer. Nature (2015) 3.77

Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis. Cell (2015) 3.58

Genetic and functional studies implicate HIF1α as a 14q kidney cancer suppressor gene. Cancer Discov (2011) 3.45

TScratch: a novel and simple software tool for automated analysis of monolayer wound healing assays. Biotechniques (2009) 3.35

CRISPR-mediated direct mutation of cancer genes in the mouse liver. Nature (2014) 3.28

Defective placental vasculogenesis causes embryonic lethality in VHL-deficient mice. Proc Natl Acad Sci U S A (1997) 3.27

Sunitinib inhibition of Stat3 induces renal cell carcinoma tumor cell apoptosis and reduces immunosuppressive cells. Cancer Res (2009) 3.07

Hypoxia-inducible factor-1-dependent repression of E-cadherin in von Hippel-Lindau tumor suppressor-null renal cell carcinoma mediated by TCF3, ZFHX1A, and ZFHX1B. Cancer Res (2006) 3.05

Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor. Cancer Res (2006) 3.04

von Hippel-Lindau disease: a clinical and scientific review. Eur J Hum Genet (2011) 2.45

Regulation of E-cadherin expression by VHL and hypoxia-inducible factor. Cancer Res (2006) 2.24

CSF1R signaling blockade stanches tumor-infiltrating myeloid cells and improves the efficacy of radiotherapy in prostate cancer. Cancer Res (2013) 2.19

Direct and differential suppression of myeloid-derived suppressor cell subsets by sunitinib is compartmentally constrained. Cancer Res (2010) 1.92

Generation of a mouse model of Von Hippel-Lindau kidney disease leading to renal cancers by expression of a constitutively active mutant of HIF1α. Cancer Res (2011) 1.75

Clinical effect and future considerations for molecularly-targeted therapy in renal cell carcinoma. Urol Oncol (2008) 1.63

Tumor biology and prognostic factors in renal cell carcinoma. Oncologist (2011) 1.62

Hypoxia-inducible factor 1 alpha in clear cell renal cell carcinoma. Clin Cancer Res (2007) 1.61

Inhibition of CSF-1 receptor improves the antitumor efficacy of adoptive cell transfer immunotherapy. Cancer Res (2013) 1.57

EMT: a new vision of hypoxia promoting cancer progression. Cancer Biol Ther (2011) 1.54

Periostin expression and epithelial-mesenchymal transition in cancer: a review and an update. Virchows Arch (2011) 1.49

Hypoxia-dependent modification of collagen networks promotes sarcoma metastasis. Cancer Discov (2013) 1.44

The role of CXCR2/CXCR2 ligand biological axis in renal cell carcinoma. J Immunol (2005) 1.44

Von Hippel-Lindau (VHL) inactivation in sporadic clear cell renal cancer: associations with germline VHL polymorphisms and etiologic risk factors. PLoS Genet (2011) 1.43

Suppression of prostate cancer nodal and systemic metastasis by blockade of the lymphangiogenic axis. Cancer Res (2008) 1.38

Molecular aspects of renal cell carcinoma: a review. Am J Cancer Res (2010) 1.25

Targeting molecular and cellular inhibitory mechanisms for improvement of antitumor memory responses reactivated by tumor cell vaccine. J Immunol (2007) 1.14

A murine renal cell carcinoma. J Natl Cancer Inst (1973) 1.09

To "grow" or "go": TMEM16A expression as a switch between tumor growth and metastasis in SCCHN. Clin Cancer Res (2014) 1.05

Expression of MUC1 (EMA) and E-cadherin in renal cell carcinoma: a systematic immunohistochemical analysis of 188 cases. Mod Pathol (2004) 1.05

NF-kappaB-dependent plasticity of the epithelial to mesenchymal transition induced by Von Hippel-Lindau inactivation in renal cell carcinomas. Cancer Res (2010) 1.03

The 20th anniversary of interleukin-2 therapy: bimodal role explaining longstanding random induction of complete clinical responses. Cancer Manag Res (2012) 1.02

Bap1 is essential for kidney function and cooperates with Vhl in renal tumorigenesis. Proc Natl Acad Sci U S A (2014) 0.97

Combined mutation of Vhl and Trp53 causes renal cysts and tumours in mice. EMBO Mol Med (2013) 0.97

BootstRatio: A web-based statistical analysis of fold-change in qPCR and RT-qPCR data using resampling methods. Comput Biol Med (2012) 0.91

Deletions of chromosomes 3p and 14q molecularly subclassify clear cell renal cell carcinoma. Cancer (2013) 0.88

Conditional inactivation of the mouse von Hippel-Lindau tumor suppressor gene results in wide-spread hyperplastic, inflammatory and fibrotic lesions in the kidney. Oncogene (2014) 0.87

Detailed genetic mapping of the von Hippel-Lindau disease tumour suppressor gene. J Med Genet (1993) 0.87

Periostin Is a Key Niche Component for Wound Metastasis of Melanoma. PLoS One (2015) 0.85

Periostin is a novel therapeutic target that predicts and regulates glioma malignancy. Neuro Oncol (2014) 0.83

SAMSN1 is highly expressed and associated with a poor survival in glioblastoma multiforme. PLoS One (2013) 0.80

Modeling spontaneous metastatic renal cell carcinoma (mRCC) in mice following nephrectomy. J Vis Exp (2014) 0.78

Increased B cell-activating factor promotes tumor invasion and metastasis in human pancreatic cancer. PLoS One (2013) 0.78

TGFβ3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis. Sci Rep (2016) 0.77