In vivo formation of hybrid toxins comprising Shiga toxin and the Shiga-like toxins and role of the B subunit in localization and cytotoxic activity.

PubWeight™: 1.69‹?› | Rank: Top 3%

🔗 View Article (PMC 259899)

Published in Infect Immun on December 01, 1989

Authors

D L Weinstein1, M P Jackson, L P Perera, R K Holmes, A D O'Brien

Author Affiliations

1: Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.

Articles citing this

Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin Microbiol Rev (1998) 8.74

Comparison of the relative toxicities of Shiga-like toxins type I and type II for mice. Infect Immun (1993) 3.36

Acute renal tubular necrosis and death of mice orally infected with Escherichia coli strains that produce Shiga-like toxin type II. Infect Immun (1990) 3.02

Comparison of the glycolipid receptor specificities of Shiga-like toxin type II and Shiga-like toxin type II variants. Infect Immun (1990) 2.32

Analysis of Escherichia coli O157:H7 survival in ovine or bovine manure and manure slurry. Appl Environ Microbiol (1998) 2.13

Escherichia coli O157:H7 in microbial flora of sheep. J Clin Microbiol (1996) 1.83

Characterization of Escherichia coli O157:H7 and other Shiga toxin-producing E. coli serotypes isolated from sheep. J Clin Microbiol (1997) 1.77

Transcription of the Shiga-like toxin type II and Shiga-like toxin type II variant operons of Escherichia coli. J Bacteriol (1990) 1.39

Evaluation of a technique for identification of Shiga-like toxin-producing Escherichia coli by using polymerase chain reaction and digoxigenin-labeled probes. J Clin Microbiol (1993) 1.30

Functional analysis of the Shiga toxin and Shiga-like toxin type II variant binding subunits by using site-directed mutagenesis. J Bacteriol (1990) 1.24

The CI repressors of Shiga toxin-converting prophages are involved in coinfection of Escherichia coli strains, which causes a down regulation in the production of Shiga toxin 2. J Bacteriol (2008) 1.13

Identification of three amino acid residues in the B subunit of Shiga toxin and Shiga-like toxin type II that are essential for holotoxin activity. J Bacteriol (1991) 1.07

Mutational analysis of the Shiga toxin and Shiga-like toxin II enzymatic subunits. J Bacteriol (1990) 1.06

Shiga toxin 2 is specifically released from bacterial cells by two different mechanisms. Infect Immun (2009) 1.01

Shiga toxin binding to glycolipids and glycans. PLoS One (2012) 0.98

Comparison of binding platforms yields insights into receptor binding differences between shiga toxins 1 and 2. Biochemistry (2010) 0.95

Syntheses and immunologic properties of Escherichia coli O157 O-specific polysaccharide and Shiga toxin 1 B subunit conjugates in mice. Infect Immun (1999) 0.95

Molecular basis of differential B-pentamer stability of Shiga toxins 1 and 2. PLoS One (2010) 0.95

A plant-based oral vaccine to protect against systemic intoxication by Shiga toxin type 2. Proc Natl Acad Sci U S A (2006) 0.93

New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo. PLoS One (2014) 0.91

Minimum domain of the Shiga toxin A subunit required for enzymatic activity. J Bacteriol (1993) 0.91

Analysis of Shiga toxin subunit association by using hybrid A polypeptides and site-specific mutagenesis. J Bacteriol (1995) 0.91

Do the A subunits contribute to the differences in the toxicity of Shiga toxin 1 and Shiga toxin 2? Toxins (Basel) (2015) 0.86

Glycolipid binding preferences of Shiga toxin variants. PLoS One (2014) 0.85

Mapping the minimal contiguous gene segment that encodes functionally active Shiga-like toxin II. Infect Immun (1991) 0.82

The A1 Subunit of Shiga Toxin 2 Has Higher Affinity for Ribosomes and Higher Catalytic Activity than the A1 Subunit of Shiga Toxin 1. Infect Immun (2015) 0.81

Binding of Pk-trisaccharide analogs of globotriaosylceramide to Shiga toxin variants. Infect Immun (2013) 0.81

Comparisons of native Shiga toxins (Stxs) type 1 and 2 with chimeric toxins indicate that the source of the binding subunit dictates degree of toxicity. PLoS One (2014) 0.81

The serine 31 residue of the B subunit of Shiga toxin 2 is essential for secretion in enterohemorrhagic Escherichia coli. Infect Immun (2007) 0.81

Structural basis of subtilase cytotoxin SubAB assembly. J Biol Chem (2013) 0.79

Roles of a ribosome-binding site and mRNA secondary structure in differential expression of Shiga toxin genes. J Bacteriol (1993) 0.78

Reduced Toxicity of Shiga Toxin (Stx) Type 2c in Mice Compared to Stx2d Is Associated with Instability of Stx2c Holotoxin. Toxins (Basel) (2015) 0.76

Articles cited by this

Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (1970) 1528.65

DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A (1977) 790.54

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol (1978) 75.38

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol (1980) 61.16

Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A (1983) 54.64

Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template. DNA (1984) 14.55

Two toxin-converting phages from Escherichia coli O157:H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infect Immun (1986) 8.09

Production of Shigella dysenteriae type 1-like cytotoxin by Escherichia coli. J Infect Dis (1982) 8.05

Quantitative microtiter cytotoxicity assay for Shigella toxin. J Clin Microbiol (1980) 7.68

Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine. J Bacteriol (1988) 5.43

Site of action of a Vero toxin (VT2) from Escherichia coli O157:H7 and of Shiga toxin on eukaryotic ribosomes. RNA N-glycosidase activity of the toxins. Eur J Biochem (1988) 5.22

Characterization of monoclonal antibodies against Shiga-like toxin from Escherichia coli. Infect Immun (1985) 5.04

RNA N-glycosidase activity of ricin A-chain. Mechanism of action of the toxic lectin ricin on eukaryotic ribosomes. J Biol Chem (1987) 4.72

Construction and characterization of new cloning vehicles. VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication. Gene (1982) 4.36

Cloning and sequencing of the genes for Shiga toxin from Shigella dysenteriae type 1. J Bacteriol (1988) 3.98

Purification and characterization of a Shigella dysenteriae 1-like toxin produced by Escherichia coli. Infect Immun (1983) 3.85

Glycolipid binding of purified and recombinant Escherichia coli produced verotoxin in vitro. J Biol Chem (1987) 3.71

Cloning of Shiga-like toxin structural genes from a toxin converting phage of Escherichia coli. Science (1985) 3.66

Pathogenesis of shigella diarrhea. XI. Isolation of a shigella toxin-binding glycolipid from rabbit jejunum and HeLa cells and its identification as globotriaosylceramide. J Exp Med (1986) 3.61

Cloning of genes determining the production of vero cytotoxin by Escherichia coli. J Gen Microbiol (1985) 3.60

Production of Shiga-like toxin by Escherichia coli. J Infect Dis (1986) 3.53

Pathogenesis of Shigella diarrhea. IX. Simplified high yield purification of Shigella toxin and characterization of subunit composition and function by the use of subunit-specific monoclonal and polyclonal antibodies. J Exp Med (1984) 3.23

The cytotoxic activity of Shigella toxin. Evidence for catalytic inactivation of the 60 S ribosomal subunit. J Biol Chem (1981) 3.13

Identification of the carbohydrate receptor for Shiga toxin produced by Shigella dysenteriae type 1. J Biol Chem (1987) 3.00

Affinity purification and characterization of Shiga-like toxin II and production of toxin-specific monoclonal antibodies. Infect Immun (1988) 2.99

Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J Clin Microbiol (1988) 2.76

Globotriosyl ceramide is specifically recognized by the Escherichia coli verocytotoxin 2. Biochem Biophys Res Commun (1988) 2.65

Subunit structure of Shigella cytotoxin. J Biol Chem (1981) 2.59

Shiga toxin, Shiga-like toxin II variant, and ricin are all single-site RNA N-glycosidases of 28 S RNA when microinjected into Xenopus oocytes. J Biol Chem (1989) 2.12

Cloning of genes for production of Escherichia coli Shiga-like toxin type II. Infect Immun (1987) 2.04

Nucleotide sequence of cloned cDNA coding for preproricin. Eur J Biochem (1985) 1.82

Isolation and some properties of A and B subunits of Vero toxin 2 and in vitro formation of hybrid toxins between subunits of Vero toxin 1 and Vero toxin 2 from Escherichia coli O157:H7. Microb Pathog (1988) 0.98

Articles by these authors

The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesiveness factor. J Infect Dis (1985) 9.18

Shiga and Shiga-like toxins. Microbiol Rev (1987) 8.58

Two toxin-converting phages from Escherichia coli O157:H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infect Immun (1986) 8.09

Production of Shigella dysenteriae type 1-like cytotoxin by Escherichia coli. J Infect Dis (1982) 8.05

Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea. Science (1984) 7.94

Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine. J Bacteriol (1988) 5.43

Genetic control of the innate resistance of mice to Salmonella typhimurium: expression of the Ity gene in peritoneal and splenic macrophages isolated in vitro. J Immunol (1983) 5.22

Characterization of monoclonal antibodies against Shiga-like toxin from Escherichia coli. Infect Immun (1985) 5.04

Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157:H- strain E32511. Infect Immun (1991) 4.63

The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model. J Clin Invest (1993) 4.11

Studies on toxinogenesis in Vibrio cholerae. I. Isolation of mutants with altered toxinogenicity. J Infect Dis (1974) 4.02

Cloning and sequencing of the genes for Shiga toxin from Shigella dysenteriae type 1. J Bacteriol (1988) 3.98

Role of a 60-megadalton plasmid and Shiga-like toxins in the pathogenesis of infection caused by enterohemorrhagic Escherichia coli O157:H7 in gnotobiotic piglets. Infect Immun (1987) 3.96

Purification and characterization of a Shigella dysenteriae 1-like toxin produced by Escherichia coli. Infect Immun (1983) 3.85

A plasmid of enterohemorrhagic Escherichia coli O157:H7 is required for expression of a new fimbrial antigen and for adhesion to epithelial cells. Infect Immun (1987) 3.76

Adherence of bacteria to heart valves in vitro. J Clin Invest (1975) 3.74

Cloning of Shiga-like toxin structural genes from a toxin converting phage of Escherichia coli. Science (1985) 3.66

Production of Shiga-like toxin by Escherichia coli. J Infect Dis (1986) 3.53

Comparison of the relative toxicities of Shiga-like toxins type I and type II for mice. Infect Immun (1993) 3.36

Genetic control of susceptibility to Salmonella typhimurium in mice: role of the LPS gene. J Immunol (1980) 3.31

Nucleotide sequence analysis of the structural genes for Shiga-like toxin I encoded by bacteriophage 933J from Escherichia coli. Microb Pathog (1987) 3.25

Studies on toxinogenesis in Vibrio cholerae. III. Characterization of nontoxinogenic mutants in vitro and in experimental animals. J Clin Invest (1975) 3.12

Characterization of hapR, a positive regulator of the Vibrio cholerae HA/protease gene hap, and its identification as a functional homologue of the Vibrio harveyi luxR gene. Mol Microbiol (1997) 3.06

Environmental and human isolates of Vibrio cholerae and Vibrio parahaemolyticus produce a Shigella dysenteriae 1 (Shiga)-like cytotoxin. Lancet (1984) 3.05

Shiga toxin: biochemistry, genetics, mode of action, and role in pathogenesis. Curr Top Microbiol Immunol (1992) 3.03

Acute renal tubular necrosis and death of mice orally infected with Escherichia coli strains that produce Shiga-like toxin type II. Infect Immun (1990) 3.02

Mouse model for colonization and disease caused by enterohemorrhagic Escherichia coli O157:H7. Infect Immun (1990) 3.00

Biological properties of Shigella flexneri 2A toxin and its serological relationship to Shigella dysenteriae 1 toxin. Infect Immun (1977) 2.94

Iron-dependent regulation of diphtheria toxin and siderophore expression by the cloned Corynebacterium diphtheriae repressor gene dtxR in C. diphtheriae C7 strains. Infect Immun (1991) 2.93

Characterization of Shigella dysenteriae 1 (Shiga) toxin purified by anti-Shiga toxin affinity chromatography. Infect Immun (1980) 2.82

Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZ alpha and pUC18 or pUC19 multiple cloning sites. Nucleic Acids Res (1990) 2.77

Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J Clin Microbiol (1988) 2.76

Genetic analysis of tox+ and tox- bacteriophages of Corynebacterium diphtheriae. J Virol (1969) 2.71

Enterohemorrhagic Escherichia coli O157:H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells. Infect Immun (1995) 2.69

Salmonella typhimurium translocates flagellin across intestinal epithelia, inducing a proinflammatory response. J Clin Invest (2001) 2.67

Quantitative measurements of cholera enterotoxin in cultures of toxinogenic wild-type and nontoxinogenic mutant strains of Vibrio cholerae by using a sensitive and specific reversed passive hemagglutination assay for cholera enerotoxin. Infect Immun (1978) 2.55

The pathogenic mechanisms of Shiga toxin and the Shiga-like toxins. Mol Microbiol (1991) 2.54

Characterization of monoclonal antibodies that react with unique and cross-reacting determinants of cholera enterotoxin and its subunits. Infect Immun (1983) 2.52

Immunochemical and cytotoxic activities of Shigella dysenteriae 1 (shiga) and shiga-like toxins. Infect Immun (1982) 2.50

Conjugal transfer of a chromosomal gene determining production of enterotoxin in vibrio cholerae. Science (1975) 2.47

Virulence of enterohemorrhagic Escherichia coli O91:H21 clinical isolates in an orally infected mouse model. Infect Immun (1993) 2.38

Absence of all components of the flagellar export and synthesis machinery differentially alters virulence of Salmonella enterica serovar Typhimurium in models of typhoid fever, survival in macrophages, tissue culture invasiveness, and calf enterocolitis. Infect Immun (2001) 2.38

Escherichia coli O157:H7 requires intimin for enteropathogenicity in calves. Infect Immun (1998) 2.37

Characterization and genetic mapping of nontoxinogenic (tox) mutants of corynebacteriophage beta. J Virol (1976) 2.33

Comparison of the glycolipid receptor specificities of Shiga-like toxin type II and Shiga-like toxin type II variants. Infect Immun (1990) 2.32

Comparison of the carbohydrate-binding specificities of cholera toxin and Escherichia coli heat-labile enterotoxins LTh-I, LT-IIa, and LT-IIb. Infect Immun (1988) 2.32

Are the Lsh and Ity disease resistance genes at one locus on mouse chromosome 1? Nature (1982) 2.29

Membrane traffic and the cellular uptake of cholera toxin. Biochim Biophys Acta (1999) 2.27

Cloning, sequence, and footprint analysis of two promoter/operators from Corynebacterium diphtheriae that are regulated by the diphtheria toxin repressor (DtxR) and iron. J Bacteriol (1994) 2.19

Flagella of Salmonella typhimurium are a virulence factor in infected C57BL/6J mice. Infect Immun (1984) 2.15

Shiga toxin, Shiga-like toxin II variant, and ricin are all single-site RNA N-glycosidases of 28 S RNA when microinjected into Xenopus oocytes. J Biol Chem (1989) 2.12

Cytotoxic necrotizing factor type 2 produced by virulent Escherichia coli modifies the small GTP-binding proteins Rho involved in assembly of actin stress fibers. Proc Natl Acad Sci U S A (1994) 2.11

Activation of Shiga-like toxins by mouse and human intestinal mucus correlates with virulence of enterohemorrhagic Escherichia coli O91:H21 isolates in orally infected, streptomycin-treated mice. Infect Immun (1996) 2.04

Genetic mapping of mutations in independently isolated nontoxinogenic mutants of Vibrio cholerae. Infect Immun (1978) 1.98

Flagella help Salmonella typhimurium survive within murine macrophages. Infect Immun (1984) 1.95

Genetic control of resistance to infection in mice. Crit Rev Immunol (1982) 1.93

Characterization of an iron-dependent regulatory protein (IdeR) of Mycobacterium tuberculosis as a functional homolog of the diphtheria toxin repressor (DtxR) from Corynebacterium diphtheriae. Infect Immun (1995) 1.92

Shiga-like toxin production from Escherichia coli associated with calf diarrhoea. Vet Rec (1985) 1.91

Effect of silica on the innate resistance of inbred mice to Salmonella typhimurium infection. Infect Immun (1979) 1.91

Ganglioside structure dictates signal transduction by cholera toxin and association with caveolae-like membrane domains in polarized epithelia. J Cell Biol (1998) 1.90

Control of early Salmonella typhimurium growth in innately Salmonella-resistant mice does not require functional T lymphocytes. J Immunol (1982) 1.90

Susceptibility of CBA/N mice to infection with Salmonella typhimurium: influence of the X-linked gene controlling B lymphocyte function. J Immunol (1979) 1.90

Purification of Shigella dysenteriae 1 (Shiga)-like toxin from Escherichia coli O157:H7 strain associated with haemorrhagic colitis. Lancet (1983) 1.87

Targeting of cholera toxin and Escherichia coli heat labile toxin in polarized epithelia: role of COOH-terminal KDEL. J Cell Biol (1995) 1.86

A BALB/c congenic strain of mice that carries a genetic locus (Ityr) controlling resistance to intracellular parasites. Infect Immun (1983) 1.86

Cellular release of heat-labile enterotoxin of Escherichia coli by bacteriophage induction. Infect Immun (1978) 1.85

Enzymatic assay for gentamicin and related aminoglycoside antibiotics. J Infect Dis (1974) 1.83

Coordinate regulation of siderophore and diphtheria toxin production by iron in Corynebacterium diphtheriae. Microb Pathog (1990) 1.83

Control of natural resistance to Salmonella typhimurium and Leishmania donovani in mice by closely linked but distinct genetic loci. Nature (1980) 1.83

Characterization of a defective diphtheria toxin repressor (dtxR) allele and analysis of dtxR transcription in wild-type and mutant strains of Corynebacterium diphtheriae. Infect Immun (1991) 1.79

Purification and some properties of a Vero toxin from a human strain of Escherichia coli that is immunologically related to Shiga-like toxin II (VT2). Microb Pathog (1989) 1.78

Cloning of genes that encode a new heat-labile enterotoxin of Escherichia coli. J Bacteriol (1986) 1.77

Resistance of Pseudomonas aeruginosa to gentamicin and related aminoglycoside antibiotics. Antimicrob Agents Chemother (1974) 1.77

Shiga-like toxin production and attaching effacing activity of Escherichia coli associated with calf diarrhea. Am J Vet Res (1987) 1.75

Genetically conferred defect in anti-Salmonella antibody formation renders CBA/N mice innately susceptible to Salmonella typhimurium infection. J Immunol (1981) 1.75

Evaluation of the role of Shiga and Shiga-like toxins in mediating direct damage to human vascular endothelial cells. J Infect Dis (1991) 1.73

Morphologic evaluation of the effects of Shiga toxin and E coli Shiga-like toxin on the rabbit intestine. Am J Pathol (1986) 1.72

Studies on toxinogenesis in Vibrio cholerae. II. An vitro test for enterotoxin production. Infect Immun (1974) 1.72

Synthesis and secretion of the plasmid-coded heat-labile enterotoxin of Escherichia coli in Vibrio cholerae. Science (1983) 1.70

The specific activities of Shiga-like toxin type II (SLT-II) and SLT-II-related toxins of enterohemorrhagic Escherichia coli differ when measured by Vero cell cytotoxicity but not by mouse lethality. Infect Immun (1994) 1.70

Purification and characterization of the diphtheria toxin repressor. Proc Natl Acad Sci U S A (1992) 1.66

Regulation of toxinogenesis in Corynebacterium diphtheriae. I. Mutations in bacteriophage beta that alter the effects of iron on toxin production. J Virol (1981) 1.65

Transcriptional control of the iron-responsive fxbA gene by the mycobacterial regulator IdeR. J Bacteriol (1999) 1.65

Mutation of the gene encoding cytotoxic necrotizing factor type 1 (cnf(1)) attenuates the virulence of uropathogenic Escherichia coli. Infect Immun (2001) 1.64

Evidence that a new enterotoxin of Escherichia coli which activates adenylate cyclase in eucaryotic target cells is not plasmid mediated. Infect Immun (1983) 1.64

A Salmonella typhimurium virulence gene linked to flg. Infect Immun (1989) 1.64