Published in Biochemistry on September 23, 1986
The second extracellular loop of the dopamine D2 receptor lines the binding-site crevice. Proc Natl Acad Sci U S A (2004) 2.10
Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus. Proc Natl Acad Sci U S A (2001) 2.03
State-dependent accessibility and electrostatic potential in the channel of the acetylcholine receptor. Inferences from rates of reaction of thiosulfonates with substituted cysteines in the M2 segment of the alpha subunit. J Gen Physiol (1998) 1.99
Identification of channel-lining residues in the M2 membrane-spanning segment of the GABA(A) receptor alpha1 subunit. J Gen Physiol (1996) 1.85
Crystal structure of a theta-class glutathione transferase. EMBO J (1995) 1.75
Acetylcholine receptor channel structure in the resting, open, and desensitized states probed with the substituted-cysteine-accessibility method. Proc Natl Acad Sci U S A (2001) 1.73
The intrinsic electrostatic potential and the intermediate ring of charge in the acetylcholine receptor channel. J Gen Physiol (2000) 1.56
Identification of cystic fibrosis transmembrane conductance regulator channel-lining residues in and flanking the M6 membrane-spanning segment. Biophys J (1996) 1.54
Locating the anion-selectivity filter of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel. J Gen Physiol (1997) 1.49
A conformation change in the extracellular domain that accompanies desensitization of acid-sensing ion channel (ASIC) 3. J Gen Physiol (2007) 1.38
Lateral gene transfer and parallel evolution in the history of glutathione biosynthesis genes. Genome Biol (2002) 1.35
Structure of the M2 transmembrane segment of GLIC, a prokaryotic Cys loop receptor homologue from Gloeobacter violaceus, probed by substituted cysteine accessibility. J Biol Chem (2011) 1.33
Gamma-aminobutyric acid increases the water accessibility of M3 membrane-spanning segment residues in gamma-aminobutyric acid type A receptors. Biophys J (1999) 1.32
Cocaine alters the accessibility of endogenous cysteines in putative extracellular and intracellular loops of the human dopamine transporter. Proc Natl Acad Sci U S A (1998) 1.28
Agonist-, antagonist-, and benzodiazepine-induced structural changes in the alpha1 Met113-Leu132 region of the GABAA receptor. Mol Pharmacol (2006) 1.24
Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking. Proc Natl Acad Sci U S A (2002) 1.22
Structural interactions between transmembrane helices 4 and 5 of subunit a and the subunit c ring of Escherichia coli ATP synthase. J Biol Chem (2008) 1.21
Subunit a facilitates aqueous access to a membrane-embedded region of subunit c in Escherichia coli F1F0 ATP synthase. J Biol Chem (2008) 1.14
Intermolecular thiol cross-linking via loops in the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A (2003) 1.11
CFTR: a cysteine at position 338 in TM6 senses a positive electrostatic potential in the pore. Biophys J (2004) 1.08
Interaction of recombinant human cystatin C with the cysteine proteinases papain and actinidin. Biochem J (1992) 1.06
Interaction of chicken cystatin with inactivated papains. Biochem J (1989) 1.01
The cytoplasmic loops of subunit a of Escherichia coli ATP synthase may participate in the proton translocating mechanism. J Biol Chem (2008) 1.01
Methylsulfonyl benzothiazole (MSBT): a selective protein thiol blocking reagent. Org Lett (2012) 1.00
A potent, versatile disulfide-reducing agent from aspartic acid. J Am Chem Soc (2012) 1.00
Reactions of cysteines substituted in the amphipathic N-terminal tail of a bacterial potassium channel with hydrophilic and hydrophobic maleimides. Proc Natl Acad Sci U S A (2002) 0.99
Residues accessible in the binding-site crevice of transmembrane helix 6 of the CB2 cannabinoid receptor. Biochemistry (2008) 0.97
Purification and characterization of [acyl-carrier-protein] acetyltransferase from Escherichia coli. Biochem J (1988) 0.96
Characterization by rapid-kinetic and equilibrium methods of the interaction between N-terminally truncated forms of chicken cystatin and the cysteine proteinases papain and actinidin. Biochem J (1992) 0.95
Chemical reactivities of cysteine substitutions in subunit a of ATP synthase define residues gating H+ transport from each side of the membrane. J Biol Chem (2010) 0.94
Thiol-blocking electrophiles interfere with labeling and detection of protein sulfenic acids. FEBS J (2013) 0.94
Mass spectral characterization of organophosphate-labeled, tyrosine-containing peptides: characteristic mass fragments and a new binding motif for organophosphates. J Chromatogr B Analyt Technol Biomed Life Sci (2009) 0.92
Papain labelled with fluorescent thiol-specific reagents as a probe for characterization of interactions between cysteine proteinases and their protein inhibitors by competitive titrations. Biochem J (1991) 0.91
Scam feels the pinch. J Gen Physiol (2001) 0.90
Transmembrane segment 11 appears to line the purine permeation pathway of the Plasmodium falciparum equilibrative nucleoside transporter 1 (PfENT1). J Biol Chem (2010) 0.89
Evidence by chemical modification that tryptophan-104 of the cysteine-proteinase inhibitor chicken cystatin is located in or near the proteinase-binding site. Biochem J (1990) 0.88
Tethered agonist analogs as site-specific probes for domains of the human α7 nicotinic acetylcholine receptor that differentially regulate activation and desensitization. Mol Pharmacol (2010) 0.87
Probing protein packing surrounding the residues in and flanking the nicotinic acetylcholine receptor M2M3 loop. J Neurosci (2009) 0.86
Conformational changes in the lower palm domain of ASIC1a contribute to desensitization and RFamide modulation. PLoS One (2013) 0.86
CHARMM-GUI PDB manipulator for advanced modeling and simulations of proteins containing nonstandard residues. Adv Protein Chem Struct Biol (2014) 0.86
Synthesis of GABAA receptor agonists and evaluation of their alpha-subunit selectivity and orientation in the GABA binding site. J Med Chem (2008) 0.85
Identification of channel-lining amino acid residues in the hydrophobic segment of colicin Ia. J Gen Physiol (2008) 0.85
Differential roles of cysteine residues in the cellular trafficking, dimerization, and function of the high-density lipoprotein receptor, SR-BI. Biochemistry (2011) 0.83
Activation of methyltetrahydrofolate by cobalamin-independent methionine synthase. Biochemistry (2006) 0.83
Probing the structural and functional domains of the CFTR chloride channel. J Bioenerg Biomembr (1997) 0.82
Delineating the extracellular water-accessible surface of the proton-coupled folate transporter. PLoS One (2013) 0.82
The seventh transmembrane domains of the delta and kappa opioid receptors have different accessibility patterns and interhelical interactions. Biochemistry (2005) 0.81
Analysis of transmembrane domains 1 and 4 of the human angiotensin II AT1 receptor by cysteine-scanning mutagenesis. J Biol Chem (2009) 0.81
A theoretical study of the active sites of papain and S195C rat trypsin: implications for the low reactivity of mutant serine proteinases. Protein Sci (1996) 0.80
Structural studies of the actions of anesthetic drugs on the γ-aminobutyric acid type A receptor. Anesthesiology (2011) 0.80
Voltage-dependent conformational changes of KVAP S4 segment in bacterial membrane environment. Channels (Austin) (2009) 0.79
High-affinity binding of two molecules of cysteine proteinases to low-molecular-weight kininogen. Protein Sci (1995) 0.77
Mapping peptide thiol accessibility in membranes using a quaternary ammonium isotope-coded mass tag (ICMT). Bioconjug Chem (2013) 0.76
Modulation of the reactivity of the essential cysteine residue of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa. Biochem J (2002) 0.76
Direct evidence that scorpion α-toxins (site-3) modulate sodium channel inactivation by hindrance of voltage-sensor movements. PLoS One (2013) 0.75
Caution is required in interpretation of mutations in the voltage sensing domain of voltage gated channels as evidence for gating mechanisms. Int J Mol Sci (2015) 0.75
Mapping the membrane topography of the TH6-TH7 segment of the diphtheria toxin T-domain channel. J Gen Physiol (2015) 0.75
Glutathione transferase mimics: micellar catalysis of an enzymic reaction. Biochem J (1997) 0.75
Photoactivated gamma-secretase inhibitors directed to the active site covalently label presenilin 1. Nature (2000) 4.12
Many pulmonary pathogenic bacteria bind specifically to the carbohydrate sequence GalNAc beta 1-4Gal found in some glycolipids. Proc Natl Acad Sci U S A (1988) 3.83
Thrombospondin binds falciparum malaria parasitized erythrocytes and may mediate cytoadherence. Nature (1985) 3.53
Presenilin 1 is linked with gamma-secretase activity in the detergent solubilized state. Proc Natl Acad Sci U S A (2000) 3.13
Oxidative protein folding is driven by the electron transport system. Cell (1999) 3.09
Improved DNA: liposome complexes for increased systemic delivery and gene expression. Nat Biotechnol (1997) 2.52
Pseudomonas aeruginosa and Pseudomonas cepacia isolated from cystic fibrosis patients bind specifically to gangliotetraosylceramide (asialo GM1) and gangliotriaosylceramide (asialo GM2). Arch Biochem Biophys (1988) 2.49
Regulation of transforming growth factor-beta activation by discrete sequences of thrombospondin 1. J Biol Chem (1995) 2.28
Transfection of thrombospondin 1 complementary DNA into a human breast carcinoma cell line reduces primary tumor growth, metastatic potential, and angiogenesis. Cancer Res (1994) 2.20
Phthalate dioxygenase reductase: a modular structure for electron transfer from pyridine nucleotides to [2Fe-2S]. Science (1992) 2.12
Inhibition of angiogenesis by thrombospondin-1 is mediated by 2 independent regions within the type 1 repeats. Circulation (1999) 2.11
The mechanism of action of xanthine oxidase. J Biol Chem (1974) 2.07
Cloning, expression, and regulation of the Pseudomonas cepacia protocatechuate 3,4-dioxygenase genes. J Bacteriol (1989) 2.04
The platelet glycoprotein thrombospondin binds specifically to sulfated glycolipids. J Biol Chem (1985) 1.90
A molecular basis for nitric oxide sensing by soluble guanylate cyclase. Proc Natl Acad Sci U S A (1999) 1.86
Thrombospondin 1 and type I repeat peptides of thrombospondin 1 specifically induce apoptosis of endothelial cells. Cancer Res (1997) 1.81
Platelet thrombospondin mediates attachment and spreading of human melanoma cells. J Cell Biol (1987) 1.81
Purification and characterization of phthalate oxygenase and phthalate oxygenase reductase from Pseudomonas cepacia. J Biol Chem (1987) 1.79
Adhesion of Mycoplasma pneumoniae to sulfated glycolipids and inhibition by dextran sulfate. J Biol Chem (1989) 1.76
Mechanistic studies of cyclohexanone monooxygenase: chemical properties of intermediates involved in catalysis. Biochemistry (2001) 1.67
Effect of cysteine-25 on the ionization of histidine-159 in papain as determined by proton nuclear magnetic resonance spectroscopy. Evidence for a his-159--Cys-25 ion pair and its possible role in catalysis. Biochemistry (1981) 1.64
The use of protocatechuate dioxygenase for maintaining anaerobic conditions in biochemical experiments. Anal Biochem (2000) 1.61
Flavin-oxygen derivatives involved in hydroxylation by p-hydroxybenzoate hydroxylase. J Biol Chem (1976) 1.61
Imidazole-catalyzed displacement of an amine from an amide by a neighboring hydroxyl group. A model for the acylation of chymotrypsin. J Am Chem Soc (1971) 1.59
Expression of the extracellular matrix molecule thrombospondin inversely correlates with malignant progression in melanoma, lung and breast carcinoma cell lines. Int J Cancer (1994) 1.57
Thrombospondin-induced tumor cell migration: haptotaxis and chemotaxis are mediated by different molecular domains. J Cell Biol (1987) 1.56
Electron-nuclear double resonance spectroscopy of 15N-enriched phthalate dioxygenase from Pseudomonas cepacia proves that two histidines are coordinated to the [2Fe-2S] Rieske-type clusters. Biochemistry (1989) 1.56
Separate roles for FMN and FAD in catalysis by liver microsomal NADPH-cytochrome P-450 reductase. J Biol Chem (1981) 1.56
The mobile flavin of 4-OH benzoate hydroxylase. Science (1994) 1.55
Mechanistic studies on cyclohexanone oxygenase. Biochemistry (1982) 1.54
Crystal structure at 1.9-A resolution of human immunodeficiency virus (HIV) II protease complexed with L-735,524, an orally bioavailable inhibitor of the HIV proteases. J Biol Chem (1994) 1.51
Purification of the catalytically active phosphorylated form of insulin receptor kinase by affinity chromatography with O-phosphotyrosyl-binding antibodies. Arch Biochem Biophys (1985) 1.48
Predominance of tyrosine phosphorylation of insulin receptors during the initial response of intact cells to insulin. J Biol Chem (1985) 1.45
Fluoride elimination from substrates in hydroxylation reactions catalyzed by p-hydroxybenzoate hydroxylase. J Biol Chem (1980) 1.41
Cell contact-dependent activation of alpha3beta1 integrin modulates endothelial cell responses to thrombospondin-1. Mol Biol Cell (2000) 1.41
Coupling of cobalt-carbon bond homolysis and hydrogen atom abstraction in adenosylcobalamin-dependent glutamate mutase. Biochemistry (1998) 1.40
Modulation of endothelial cell proliferation, adhesion, and motility by recombinant heparin-binding domain and synthetic peptides from the type I repeats of thrombospondin. J Cell Biochem (1993) 1.37
Identification of the omega-hydroxylase of Pseudomonas oleovorans as a nonheme iron protein requiring phospholipid for catalytic activity. Biochem Biophys Res Commun (1974) 1.35
Mechanism of heparin activation of antithrombin. Evidence for reactive center loop preinsertion with expulsion upon heparin binding. Biochemistry (1996) 1.35
Mechanism of heparin activation of antithrombin. Role of individual residues of the pentasaccharide activating sequence in the recognition of native and activated states of antithrombin. J Biol Chem (1998) 1.34
Purification and properties of protocatechuate 3,4-dioxygenase from Pseudomonas putida. A new iron to subunit stoichiometry. J Biol Chem (1981) 1.34
Binding of high affinity heparin to antithrombin III. Stopped flow kinetic studies of the binding interaction. J Biol Chem (1981) 1.33
Pro-adhesive and chemotactic activities of thrombospondin-1 for breast carcinoma cells are mediated by alpha3beta1 integrin and regulated by insulin-like growth factor-1 and CD98. J Biol Chem (1999) 1.29
Heparin-binding peptides from the type I repeats of thrombospondin. Structural requirements for heparin binding and promotion of melanoma cell adhesion and chemotaxis. J Biol Chem (1992) 1.27
Laminin binds specifically to sulfated glycolipids. Proc Natl Acad Sci U S A (1985) 1.25
Thrombospondin-1: a physiological regulator of nitric oxide signaling. Cell Mol Life Sci (2008) 1.23
Characterization of the kinetic pathway for liberation of fibrinopeptides during assembly of fibrin. J Biol Chem (1985) 1.23
Genetic organization and sequence of the Pseudomonas cepacia genes for the alpha and beta subunits of protocatechuate 3,4-dioxygenase. J Bacteriol (1989) 1.22
Binding of elastin to Staphylococcus aureus. J Biol Chem (1991) 1.21
Halogenated protocatechuates as substrates for protocatechuate dioxygenase from Pseudomonas cepacia. J Biol Chem (1983) 1.21
Binding of high affinity heparin to antithrombin III. Characterization of the protein fluorescence enhancement. J Biol Chem (1981) 1.21
Heparin- and sulfatide-binding peptides from the type I repeats of human thrombospondin promote melanoma cell adhesion. Proc Natl Acad Sci U S A (1992) 1.20
Steady state kinetic parameters for the thrombin-catalyzed conversion of human fibrinogen to fibrin. J Biol Chem (1983) 1.20
Inactivation of thrombin by antithrombin is accompanied by inactivation of regulatory exosite I. J Biol Chem (1997) 1.20
The oxidative half-reaction of liver microsomal FAD-containing monooxygenase. J Biol Chem (1981) 1.17
The COOH-terminal domain of hirudin. An exosite-directed competitive inhibitor of the action of alpha-thrombin on fibrinogen. J Biol Chem (1990) 1.15
Demonstration of a two-step reaction mechanism for inhibition of alpha-thrombin by antithrombin III and identification of the step affected by heparin. J Biol Chem (1982) 1.15
Engineering plasminogen activator inhibitor 1 mutants with increased functional stability. Biochemistry (1994) 1.15
Identification of an alpha(3)beta(1) integrin recognition sequence in thrombospondin-1. J Biol Chem (1999) 1.13
Serpin reactive center loop mobility is required for inhibitor function but not for enzyme recognition. J Biol Chem (1994) 1.13
Evidence for the participation of Cys558 and Cys559 at the active site of mercuric reductase. Biochemistry (1989) 1.12
Structure and mechanism of the iron-sulfur flavoprotein phthalate dioxygenase reductase. FASEB J (1995) 1.12
Kinetic isotope effects as probes of the mechanism of galactose oxidase. Biochemistry (1998) 1.11
Sensitivity of HIV-1 reverse transcriptase and its mutants to inhibition by azidothymidine triphosphate. Biochemistry (1994) 1.11
Antithrombin. A bloody important serpin. Adv Exp Med Biol (1997) 1.10
p-Aminobenzamidine as a fluorescent probe for the active site of serine proteases. J Biol Chem (1982) 1.10
Design and synthesis of a series of potent and orally bioavailable noncovalent thrombin inhibitors that utilize nonbasic groups in the P1 position. J Med Chem (1998) 1.08
Isoprenoid diphosphate utilization by recombinant human farnesyl:protein transferase: interactive binding between substrates and a preferred kinetic pathway. Biochemistry (1993) 1.08
Fibrinogen Petoskey, a dysfibrinogenemia characterized by replacement of Arg-A alpha 16 by a histidyl residue. Evidence for thrombin-catalyzed hydrolysis at a histidyl residue. J Biol Chem (1981) 1.08
Magnetic circular dichroism studies on the mononuclear ferrous active site of phthalate dioxygenase from Pseudomonas cepacia show a change of ligation state on substrate binding. Biochemistry (1993) 1.08
Potentiometric determination of ionizations at the active site of papain. Biochemistry (1976) 1.07
Antiproliferative and antitumor activities of D-reverse peptides derived from the second type-1 repeat of thrombospondin-1. J Pept Res (1997) 1.07
Dissociation and association of the HIV-1 protease dimer subunits: equilibria and rates. Biochemistry (1994) 1.07
pH-dependent structural changes in the active site of p-hydroxybenzoate hydroxylase point to the importance of proton and water movements during catalysis. Biochemistry (1996) 1.07
Reactions of the 4a-hydroperoxide of liver microsomal flavin-containing monooxygenase with nucleophilic and electrophilic substrates. J Biol Chem (1986) 1.06
Solution oligomerization of the rev protein of HIV-1: implications for function. Biochemistry (1993) 1.06
Deconvolution of the fluorescence emission spectrum of human antithrombin and identification of the tryptophan residues that are responsive to heparin binding. J Biol Chem (1998) 1.06
Kinetic pathway for the slow to fast transition of thrombin. Evidence of linked ligand binding at structurally distinct domains. J Biol Chem (1997) 1.06
Saturation mutagenesis of the plasminogen activator inhibitor-1 reactive center. J Biol Chem (1992) 1.05
The collagen binding domain of fibronectin contains a high affinity binding site for Candida albicans. J Biol Chem (1994) 1.05
Examination of the role of methylenetetrahydrofolate reductase in incorporation of methyltetrahydrofolate into cellular metabolism. FASEB J (1988) 1.04
Substrate recognition by "password" in p-hydroxybenzoate hydroxylase. Biochemistry (1999) 1.04
Evidence for N coordination to Fe in the [2Fe-2S] clusters of Thermus Rieske protein and phthalate dioxygenase from Pseudomonas. J Biol Chem (1985) 1.04
Purification and properties of the flavoenzyme D-lactate dehydrogenase from Megasphaera elsdenii. Biochemistry (1979) 1.04
Cloning and expression of the catA and catBC gene clusters from Pseudomonas aeruginosa PAO. J Bacteriol (1988) 1.04
An antibody against the exosite of the cloned thrombin receptor inhibits experimental arterial thrombosis in the African green monkey. Circulation (1995) 1.03
Resonance Raman studies of Rieske-type proteins. Biochim Biophys Acta (1992) 1.03
Modulation of angiogenesis by dithiolethione-modified NSAIDs and valproic acid. Br J Pharmacol (2007) 1.03
The oligosaccharide side chain on Asn-135 of alpha-antithrombin, absent in beta-antithrombin, decreases the heparin affinity of the inhibitor by affecting the heparin-induced conformational change. Biochemistry (1997) 1.03
Isolation of human blood coagulation alpha-factor Xa by soybean trypsin inhibitor-sepharose chromatography and its active-site titration with fluorescein mono-p-guanidinobenzoate. Arch Biochem Biophys (1989) 1.02
Kinetic parameters for the binding of p-nitrophenyl alpha-d-mannopyranoside to concanavalin A. Arch Biochem Biophys (1976) 1.02
Mechanism of acceleration of antithrombin-proteinase reactions by low affinity heparin. Role of the antithrombin binding pentasaccharide in heparin rate enhancement. J Biol Chem (1995) 1.02