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Top papers

Rank Title Journal Year PubWeight™‹?›
1 Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome. Proc Natl Acad Sci U S A 1978 8.36
2 Lethal disruption of the yeast actin gene by integrative DNA transformation. Science 1982 6.88
3 Directed mutagenesis. Annu Rev Genet 1981 5.27
4 Denatured states of proteins. Annu Rev Biochem 1991 4.86
5 Regulatory mutants of simian virus 40: constructed mutants with base substitutions at the origin of DNA replication. J Mol Biol 1979 3.75
6 Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene. Proc Natl Acad Sci U S A 1984 3.47
7 A genetic system for analysis of staphylococcal nuclease. Gene 1983 3.41
8 Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro. Proc Natl Acad Sci U S A 1982 2.78
9 Characterization of long-range structure in the denatured state of staphylococcal nuclease. II. Distance restraints from paramagnetic relaxation and calculation of an ensemble of structures. J Mol Biol 1997 2.38
10 Characterization of long-range structure in the denatured state of staphylococcal nuclease. I. Paramagnetic relaxation enhancement by nitroxide spin labels. J Mol Biol 1997 2.24
11 Segment-directed mutagenesis: construction in vitro of point mutations limited to a small predetermined region of a circular DNA molecule. Proc Natl Acad Sci U S A 1980 2.10
12 Genetic analysis of staphylococcal nuclease: identification of three intragenic "global" suppressors of nuclease-minus mutations. Genetics 1985 2.08
13 Directed mutagenesis with sodium bisulfite. Methods Enzymol 1983 2.07
14 Strategies and applications of in vitro mutagenesis. Science 1985 2.07
15 Kinetic and ultraviolet spectroscopic studies of active-site mutants of delta 5-3-ketosteroid isomerase. Biochemistry 1989 1.85
16 Contributions of the polar, uncharged amino acids to the stability of staphylococcal nuclease: evidence for mutational effects on the free energy of the denatured state. Biochemistry 1992 1.75
17 Truncated staphylococcal nuclease is compact but disordered. Proc Natl Acad Sci U S A 1992 1.72
18 In a staphylococcal nuclease mutant the side-chain of a lysine replacing valine 66 is fully buried in the hydrophobic core. J Mol Biol 1991 1.64
19 Mutants of simian virus 40 with base substitutions at the origin of DNA replication. Cold Spring Harb Symp Quant Biol 1979 1.58
20 Structure and dynamics of a denatured 131-residue fragment of staphylococcal nuclease: a heteronuclear NMR study. Biochemistry 1994 1.55
21 Null alleles of SAC7 suppress temperature-sensitive actin mutations in Saccharomyces cerevisiae. Mol Cell Biol 1990 1.47
22 Kinetic and magnetic resonance studies of active-site mutants of staphylococcal nuclease: factors contributing to catalysis. Biochemistry 1987 1.46
23 The equilibrium folding pathway of staphylococcal nuclease: identification of the most stable chain-chain interactions by NMR and CD spectroscopy. Biochemistry 1995 1.45
24 Accommodation of single amino acid insertions by the native state of staphylococcal nuclease. Proteins 1990 1.42
25 A general strategy for random insertion and substitution mutagenesis: substoichiometric coupling of trinucleotide phosphoramidites. Proc Natl Acad Sci U S A 1992 1.40
26 Contributions of the ionizable amino acids to the stability of staphylococcal nuclease. Biochemistry 1996 1.38
27 Patterns of nonadditivity between pairs of stability mutations in staphylococcal nuclease. Biochemistry 1993 1.33
28 Backbone dynamics of a highly disordered 131 residue fragment of staphylococcal nuclease. J Mol Biol 1994 1.30
29 Kinetic and magnetic resonance studies of effects of genetic substitution of a Ca2+-liganding amino acid in staphylococcal nuclease. Biochemistry 1986 1.24
30 Structural and energetic differences between insertions and substitutions in staphylococcal nuclease. Proteins 1992 1.12
31 Analysis of long-range interactions in a model denatured state of staphylococcal nuclease based on correlated changes in backbone dynamics. Protein Sci 1999 1.08
32 A model of the changes in denatured state structure underlying m value effects in staphylococcal nuclease. Nat Struct Biol 1999 1.07
33 NMR structure of a stable "OB-fold" sub-domain isolated from staphylococcal nuclease. J Mol Biol 1995 1.04
34 The alpha aneurism: a structural motif revealed in an insertion mutant of staphylococcal nuclease. Proc Natl Acad Sci U S A 1993 1.01
35 Initial studies of the equilibrium folding pathway of staphylococcal nuclease. Philos Trans R Soc Lond B Biol Sci 1995 1.00
36 Isolation and sequencing of the gene encoding delta 5-3-ketosteroid isomerase of Pseudomonas testosteroni: overexpression of the protein. Proc Natl Acad Sci U S A 1987 0.99
37 A dynamic bundle of four adjacent hydrophobic segments in the denatured state of staphylococcal nuclease. Protein Sci 1996 0.96
38 Guanidine hydrochloride denaturation studies of mutant forms of staphylococcal nuclease. J Cell Biochem 1986 0.95
39 Residual helical and turn structure in the denatured state of staphylococcal nuclease: analysis of peptide fragments. Fold Des 1997 0.95
40 Evidence for strained interactions between side-chains and the polypeptide backbone. J Mol Biol 1994 0.94
41 Trapping of a novel coenzyme A containing intermediate of 3-hydroxy-3-methylglutaryl-CoA synthase. Biochem Biophys Res Commun 1976 0.92
42 Triple-resonance NOESY-based experiments with improved spectral resolution: applications to structural characterization of unfolded, partially folded and folded proteins. J Biomol NMR 1997 0.91
43 Measurement of water-amide proton exchange rates in the denatured state of staphylococcal nuclease by a magnetization transfer technique. Proteins 1997 0.91
44 Comprehensive NOE characterization of a partially folded large fragment of staphylococcal nuclease Delta131Delta, using NMR methods with improved resolution. J Mol Biol 1997 0.87
45 Correlation between changes in nuclear magnetic resonance order parameters and conformational entropy: molecular dynamics simulations of native and denatured staphylococcal nuclease. Proteins 2000 0.86
46 Electrophoretic characterization of the denatured states of staphylococcal nuclease. J Mol Biol 1994 0.85
47 Diverse interactions between the individual mutations in a double mutant at the active site of staphylococcal nuclease. Biochemistry 1990 0.82
48 Perturbations of the denatured state ensemble: modeling their effects on protein stability and folding kinetics. Protein Sci 1996 0.80
49 Protein folding as seen from water's perspective. Nat Struct Biol 1999 0.79
50 A simplified protocol for isolation and characterization of ssM13 DNA templates for use in dideoxy sequencing. Biotechniques 1993 0.78
51 Protein fold recognition. Nat Struct Biol 1995 0.77
52 Alpha helix propensity of amino acids. Science 1993 0.77
53 Crystallization and preliminary X-ray analysis of a quadruple mutant of staphylococcal nuclease. J Biol Chem 1988 0.75
54 Single-stranded gaps as localized targets for in vitro mutagenesis. Basic Life Sci 1982 0.75